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- ItemSomente MetadadadosAnálise Da Expressão Da Proteína Limd2 Em Amostras De Carcinoma Papilífero Da Tiroide Metastático E Nãometastático: Correlação Com O Perfil Da Mutação V600E No Gene Braf(Universidade Federal de São Paulo (UNIFESP), 2017-01-31) Santos, Maria Jose Carregosa Pinheiro Dos [UNIFESP]; Cerutti, Janete Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Thyroid neoplasms are the most frequent neoplasms of endocrine system, being papillary thyroid carcinoma (PTC) are the most common, accounting for about 80% of all cases. Although it usually has an excellent prognosis, 30-65% of PTC cases already show metastasis at diagnosis. Alterations in genes encoding proteins from MAPK pathway, such as mutation in the BRAF gene or RET/PTC fusions, occur in about 75% of the cases of PTC. The presence of the BRAF V600E mutation is the most prevalent genetic event and has been associated with more aggressive clinical features such as the presence of metastatic lymph nodes and distant metastasis, as well as an increased risk of tumor recurrence. In an attempt to better understand the metastatic process of PTC, our group, using the methodology of SAGE (Serial Analysis of Gene Expression), has identified LIMD2 as a gene associated with the metastatic process. LIMD2 encodes a protein containing a LIM domain, and is part of a group of proteins known to be associated with important biological processes associated with metastasis, such as cytoskeletal organization, cell adhesion and motility, besides the gene transcription regulation. We investigated the expression of LIMD2 protein in a series of PTCs and lymph node metastases, and correlated with prognostic variables associated with aggressiveness and high risk of recurrence. The expression of LIMD2 was investigated in 81 cases of PTC (40 metastatic and 41 non-metastatic) and 28 paired metastases by immunohistochemistry. LIMD2 was expressed in 78% of PTC and 93% of metastases. Interestingly, most paired metastases showed a higher LIMD2 expression than primary tumors. Since the metastatic process is strongly associated with the BRAF V600E mutation in the PTC, we investigated the presence of 17 mutations in exon 15 of the BRAF gene in these samples. LIMD2 was strongly associated with the BRAF V600E mutation. However, we did not observe any association with clinico-pathological characteristics. We also analyzed the expression of LIMD2 in two PTC-derived cell lines (BCPAP and TPC-1), two follicular carcinoma-derived cell lines (FTC-133 and FTC- 238), and a normal thyroid follicular cell line (Nthy-ori 3-1). Interestingly, LIMD2 was highly expressed in BCPAP and poorly expressed in TPC-1, and was not expressed in either follicular carcinoma or normal lines. This result supports the association of LIMD2 and BRAF, since only the BCPAP line harbors the BRAF V600E mutation. In addition, our data suggest that the MAPK pathway can activate LIMD2, since TPC-1 harbors the RET/PTC1 fusion. With the recent publication of the analysis of nearly 500 PTC samples by the Cancer Genome Atlas Research Network (TCGA), a "gene signature" was associated with PTCs with BRAF V600E mutation and RET/PTC fusion, with a preferential activation of MAPK pathway, and also a signature associated with PTCs with mutations in the RAS family genes and PAX8-PPARG rearrangements, with a preferential activation of PI3K/AKT pathway. These two signatures were named BRAF-like and RAS-like, respectively. By analyzing the available data from the TCGA, we found that the expression of LIMD2 is greater in BRAF-like PTCs when compared to RAS-like PTCs. Although our data, together with the analysis of the TCGA data, suggest that the abnormal activation of the MAPK pathway increases the expression of LIMD2, further analysis needs to be done to clarify this issue. However, our data suggest that LIMD2 plays an important role in the metastatic process of PTC, especially in BRAFV600E-positive tumors.
- ItemAcesso aberto (Open Access)Avaliação da expressão imuno-histoquímica do fator de crescimento endotelial vascular (VEGF) e da podoplanina em neoplasias intraepiteliais escamosas cervicais de graus 1, 2 e 3(Universidade Federal de São Paulo (UNIFESP), 2016-07-31) Mattos, Patricia Napoli Belfort [UNIFESP]; Speck, Neila Maria de Góis [UNIFESP]; http://lattes.cnpq.br/8169544398769371; http://lattes.cnpq.br/1721457952387087; Universidade Federal de São Paulo (UNIFESP)Background: VEGF and podoplanin in invasive lesions of the cervix might beessential to restrict tumor metastatic spread. The research of these markers by immunohistochemistry can assess the immunedetection profile for diagnostic. The aim of the study was to evaluate and correlate the immunoexpression of VEGF and podoplanin in diferents degrees of cervical intraepithelial neoplasia 1, 2 and 3. Patients and Methods: two hundred thirty-four patients with cervical intraepithelial neoplasia 1, 2, 3 and controls were selected and divided em four groups. Clinical data was collected from the patients? charts and the samples from paraffin-embedded to conventional histopathologic method, construction of tissue microarray and immunohistochemical analysis. Results: We evaluated 43 controls samples, 53 samples from CIN 1 group, 60 from CIN 2 and 78 from CIN3, which the ages between 17-62 years old. Comparisons between groups to VEGF immunoexpression we observed difference statistically significant. The occurency of intense VEGF was statistically greater in CIN 2 and CIN 3 groups when compared to CIN 1 and control groups (p<0,001). The intensety of podoplanin immunoexpression between groups showed statistical significance, the negative and focal expression was more present in CIN 3 when compared to CIN 1, CIN 2 and control (p=0.016). In the immunoexpression correlation of the two factors, the podoplanin was equally frequent in different types of VEGF in control, CIN 1 and CIN 2 groups. Patients with CIN 3 presented greater frequency of VEGF moderate to intense among the patients with negative and focal podoplanin expression when compared to diffuse expression. Conclusions: The groups CIN 2 and CIN 3 appears not to present similarity in the occurrence of VEGF intense when compared to CIN 1 and control. Negative and focal podoplanin expression seems to be more present in CIN 3 when compared to other groups. Patients with CIN 3 appears to showed great tendency to moderate and intense VEGF among the patients with negative and focal podoplanin expression when compared to diffuse. .
- ItemSomente MetadadadosErythrocyte Protoporphyrin Fluorescence as a Biomarker for Monitoring Antiangiogenic Cancer Therapy(Springer, 2010-11-01) Goes Rocha, Flavia Gomes de [UNIFESP]; Barbosa Chaves, Karen Cristina [UNIFESP]; Gomes, Cinthia Zanini; Campanharo, Camila Barricheli [UNIFESP]; Courrol, Lilia Coronato [UNIFESP]; Schor, Nestor [UNIFESP]; Bellini, Maria Helena [UNIFESP]; IPEN CNEN SP; Universidade Federal de São Paulo (UNIFESP)Renal cell carcinoma (RCC) remains one of the greatest challenges of urological oncology and is the third leading cause of death in genitourinary cancers. RCCs are highly vascularized and are amenable to antiangiogenic therapy. Endostatin (ES) is a fragment of collagen XVIII that possesses antiangiogenic activity. in this study, we examined the potential of erythrocyte PpIX fluorescence spectroscopy for monitoring the efficacy of antiangiogenic therapy in metastatic renal cell carcinoma (mRCC), using an orthotopic metastatic mouse model. Balb/C-bearing Renca cells were treated with NIH/3T3-LendSN cells. Lung weight, nodule area, microvascular area (MVA), and erythrocyte PpIX fluorescence were evaluated. Emission spectra were obtained by exciting the samples at 405 nm. There was a significant decrease in lung wet weight, lung nodule area and MVA in the treated group compared to the control group (P < 0.001). Significant differences in autofluorescence shape were observed in the 620-650 nm spectral region. the most intense fluorescence peak was observed at similar to 632 nm. the autofluorescence of the control samples was about 53% higher than that of normal blood (P < 0.05). in the group treated with ES, the autofluorescence was about 54% lower than in the control group (P < 0.05). Fluorescence intensity was positively correlated with the nodule area (R (2) = 0.8859; P < 0.001) and MVA (R (2) = 0.9431; P < 0.001) in the ES-treated group. These results demonstrate that the spectroscopic analysis method allows a selective detection of tumor masses. This preliminary study suggests that PpIX fluorescence may be useful as a biomarker for antiangiogenic cancer therapy.
- ItemAcesso aberto (Open Access)Fibroistiocitoma angiomatóide com metástase ao diagnóstico: relato de caso e revisão da literatura(Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia, 2004-08-01) Heinke, Thais [UNIFESP]; Franco, Marcello Fabiano de [UNIFESP]; Alves, Maria Teresa de Seixas [UNIFESP]; Pellacani, Lucila Böhme [UNIFESP]; Gordan, Ângela Navarro [UNIFESP]; Petrilli, Antonio Sergio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Angiomatoid malignant fibrous histiocytoma is a rare and controversial entity, particularly as to its histogenesis. The tumor affects mostly the pediatric group, with an indolent clinical course and low rate of metastatization. In this report, the patient presented a lesion in the right foot and metastasis in an inguinal lymph node.
- ItemAcesso aberto (Open Access)FTY720 induces apoptosis in B16F10-NEX2 murine melanoma cells, limits metastatic development in vivo, and modulates the immune system(Faculdade de Medicina / USP, 2013-07-01) Pereira, Felipe Valença [UNIFESP]; Arruda, Denise Costa [UNIFESP]; Figueiredo, Carlos Rogerio [UNIFESP]; Massaoka, Mariana Hiromi [UNIFESP]; Matsuo, Alisson Leonardo [UNIFESP]; Bueno, Valquiria [UNIFESP]; Rodrigues, Elaine Guadelupe [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)OBJECTIVE: Available chemotherapy presents poor control over the development of metastatic melanoma. FTY720 is a compound already approved by the Food and Drug Administration for the treatment of patients with multiple sclerosis. It has also been observed that FTY720 inhibits tumor growth in vivo (experimental models) and in vitro (animal and human tumor cells). The aim of this study was to evaluate the effects of FTY720 on a metastatic melanoma model and in tumor cell lines. METHODS: We analyzed FTY720 efficacy in vivo in a syngeneic murine metastatic melanoma model, in which we injected tumor cells intravenously into C57BL/6 mice and then treated the mice orally with the compound for 7 days. We also treated mice and human tumor cell lines with FTY720 in vitro, and cell viability and death pathways were analyzed. RESULTS: FTY720 treatment limited metastatic melanoma growth in vivo and promoted a dose-dependent decrease in the viability of murine and human tumor cells in vitro. Melanoma cells treated with FTY720 exhibited characteristics of programmed cell death, reactive oxygen species generation, and increased β-catenin expression. In addition, FTY720 treatment resulted in an immunomodulatory effect in vivo by decreasing the percentage of Foxp3+ cells, without interfering with CD8+ T cells or lymphocyte-producing interferon-gamma. CONCLUSION: Further studies are needed using FTY720 as a monotherapy or in combined therapy, as different types of cancer cells would require a variety of signaling pathways to be extinguished.
- ItemAcesso aberto (Open Access)HNF4A expression as a potential diagnostic tool to discriminate primary gastric cancer from breast cancer metastasis in a Brazilian cohort(Biomed Central Ltd, 2017) de Freitas Campos Juca, Patricia Chaves; Correa, Stephany; Vignal, Giselle Maria; de Souza Accioly, Maria Theresa; Silva Lustosa, Suzana Angelica [UNIFESP]; Abdelhay, Eliana; Matos, Delcio [UNIFESP]Background: Among the many challenges in cancer diagnosis is the early distinction between metastatic cancer and a secondary tumor. This difficulty stems from the lack of markers that offer high sensitivity and specificity and can be easily applied in routine laboratory work. An example of this challenge is distinguishing gastric metastases originating from breast cancer from a gastric primary tumor. Hepatocyte nuclear factor 4 alpha (HNF4A) has been suggested as a potential marker in these cases. The aim of this study was to analyze the expression of HNF4A, estrogen receptor (ER), progesterone receptor (PR) and gross cystic disease fluid protein 15 (GCDFP-15) in a Brazilian cohort. Methods: We performed immunohistochemistry analysis of HNF4A, ER, PR and GCDFP-15 in 126 patients divided into three cohorts: primary breast cancer, primary gastric cancer and both types of tumors. Results: Our data confirmed the sensitivity and specificity of the HNF4A marker compared to other currently used clinical markers. Conclusion: HNF4A alone could be a gold standard marker for distinguishing primary gastric cancer from breast metastasis, thus validating its potential clinical use, especially in populations with high genetic diversity.
- ItemAcesso aberto (Open Access)Identificação e caracterização de marcadores relacionados à agressividade do melanoma(Universidade Federal de São Paulo (UNIFESP), 2019-09-26) Monteiro, Ana Carolina [UNIFESP]; Jasiulionis, Miriam Galvonas [UNIFESP]; http://lattes.cnpq.br/3057188718614807; http://lattes.cnpq.br/2336916559694569; Universidade Federal de São Paulo (UNIFESP)Melanoma is the most aggressive, treatment-resistant and metastatic skin cancer. It accounts for 80% of all deaths associated with these malignancies. Importantly, tumor vascularization is a critical step of the metastatic process, which is the main cause of melanoma death. Although tumor metastasis has been intensively studied in the last years, the molecular mechanisms related to it still need to be further elucidated. The murine malignant transformation model established in our laboratory was shown to be a valuable tool to study this lethal disease. Therefore, the aim of this study was to identify and define microRNAs (miRNAs) and genes related to melanoma aggressiveness in this model and to translate it to an clinical application to human patients. Initially, we further characterized the non-metastatic 4C11- cells and the metastatic 4C11+ cells, and certified that 4C11+ cells are highly aggressive in vitro and in vivo. The miRNA profile of 4C11- to 4C11+ cells identified the miR-298 as downregulated in the metastatic 4C11+ cells. In vitro assays with 4C11+ cells overexpressing this miRNA indicate its involvement in the aggressive phenotype. Moreover, this miRNA seems to regulate Angiopoietin 2 (ANGPT2), an important angiogenesis regulator. Expression analysis of cancer-related genes revealed the aggressiveness of 4C11+ cells is strongly associated with the high expression of angiogenic factors, as ANGPT2, Vascular endothelial growth factor C (VEGFC), VEGF receptor-3 (VEGFR-3) and homeobox 1 (SIX1). 5-Aza-CdR-treatment enlightened that the abnormal expression of these genes are epigenetically regulated in the analyzed murine cells. Furthermore, the inhibition of the VEGFC pathway abrogated 4C11+ cells tumorigenic potential in vitro and in vivo, suggesting this pathway has an important tumorigenic activity in these melanoma cells. Finally, TCGA data analysis revealed that ANGPT2 and VEGFR-3 expression, as well as the promotor methylation status of VEGFC, ANGPT2 and SIX1 are independent prognostic factors of overall survival in melanoma patients. The translation of the dysregulated gene expression and methylation status identified in the murine cells to a possible clinical application strongly support the applicability of our melanoma progression model to unravel new biomarkers for this aggressive human disease.
- ItemSomente MetadadadosThe Ig V-H complementarity-determining region 3-containing Rb9 peptide, inhibits melanoma cells migration and invasion by interactions with Hsp90 and an adhesion G-protein coupled receptor(Elsevier Science Inc, 2016) Girola, Natalia [UNIFESP]; Matsuo, Alisson Leonardo [UNIFESP]; Figueiredo, Carlos Rogerio [UNIFESP]; Massaoka, Mariana Hiromi [UNIFESP]; Farias, Camyla Fernandez de [UNIFESP]; Arruda, Denise C.; Azevedo, Ricardo A.; Monteiro, Hugo Pequeno [UNIFESP]; Resende-Lara, Pedro T.; Cunha, Rodrigo L. O. R.; Polonelli, Luciano; Travassos, Luiz Rodolpho [UNIFESP]The present work aims at investigating the mechanism of action of the Rb9 peptide, which contains the VHCDR 3 sequence of anti-sodium-dependent phosphate transport protein 2B (NaPi2B) monoclonal antibody RebMab200 and displayed antitumor properties. Short peptides corresponding to the hyper variable complementarity-determining regions (CDRs) of immunoglobulins have been associated with antimicrobial, antiviral, immunomodulatory and antitumor activities regardless of the specificity of the antibody. We have shown that the CDR derived peptide Rb9 induced substrate hyperadherence, inhibition of cell migration and matrix invasion in melanoma and other tumor cell lines. Rb9 also inhibited metastasis of murine melanoma in a syngeneic mouse model. We found that Rb9 binds to and interferes with Hsp90 chaperone activity causing attenuation of FAK-Src signaling and downregulation of active Rac1 in B16F10-Next melanoma cells. The peptide also bound to an adhesion G-protein coupled receptor, triggering a concentration-dependent synthesis of cAMP and activation of PKA and VASP signaling as well as IP-3 dependent Ca2+ release. Hsp90 is highly expressed on the cell surface of melanoma cells, and synthetic agents that target Hsp90 are promising cancer therapeutic drugs. Based on their remarkable antitumor effects, the CDR-H3-derived peptides from RebMab200, and particularly the highly soluble and stable Rb9, are novel candidates to be further studied as potential antitumor drugs, selectively acting on cancer cell motility and invasion. (C) 2016 Elsevier Inc. All rights reserved.
- ItemSomente MetadadadosInterplay between apoptosis and autophagy, a challenging puzzle: New perspectives on antitumor chemotherapies(Elsevier B.V., 2013-11-25) Bincoletto, C. [UNIFESP]; Bechara, A. [UNIFESP]; Pereira, G. J. S. [UNIFESP]; Santos, C. P. [UNIFESP]; Antunes, F. [UNIFESP]; da-Silva, J. Peixoto [UNIFESP]; Muler, M. [UNIFESP]; Gigli, R. D. [UNIFESP]; Monteforte, P. T. [UNIFESP]; Hirata, H. [UNIFESP]; Jurkiewicz, A. [UNIFESP]; Smaili, Soraya Soubhi [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Autophagy is a mechanism of protection against various forms of human diseases, such as cancer, in which autophagy seems to have an extremely complex role. in cancer, there is evidence that autophagy may be oncogenic in some contexts, whereas in others it clearly contributes to tumor suppression. in addition, studies have demonstrated the existence of a complex relationship between autophagy and cell death, determining whether a cell will live or die in response to anticancer therapies. Nevertheless, we still need to complete the autophagy-apoptosis puzzle in the tumor context to better address appropriate chemotherapy protocols with autophagy modulators. Generally, tumor cell resistance to anticancer induced-apoptosis can be overcome by autophagy inhibition. However, when an extensive autophagic stimulus is activated, autophagic cell death is observed. in this review, we discuss some details of autophagy and its relationship with tumor progression or suppression, as well as role of autophagy-apoptosis in cancer treatments. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
- ItemSomente MetadadadosMetastasis from Oral Cancer: An Overview(Int Inst Anticancer Research, 2012-09-01) Noguti, Juliana [UNIFESP]; Moura, Carolina Foot Gomes de [UNIFESP]; Jesus, Gustavo Protasio Pacheco de [UNIFESP]; Silva, Victor Hugo Pereira da [UNIFESP]; Hossaka, Thais Ayako [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Oral cancer is a common neoplasm worldwide. Its incidence and mortality have also increased over the past decades. It is characterized by poor prognosis and a low survival rate despite sophisticated surgical and radiotherapeutic modalities. Metastasis of oral cancer is a complex process involving detachment of cells from tumor tissue, regulation of cell motility and invasion, proliferation and evasion through the lymphatic system or blood vessels. In this review, we will focus on the current knowledge in metastasis from oral cancer regarding facts, such as incidence; stage, histopathology and grade of primary tumor; clinical manifestations; diagnosis; and treatment. Certainly, such information will contribute to the understanding of oral cancer pathogenesis.
- ItemSomente MetadadadosNeoplasia Neuroendócrina Hepática Primária E Secundária: Padrões De Imagens(Universidade Federal de São Paulo (UNIFESP), 2018-07-26) Houat, Abdallah De Paula [UNIFESP]; Atzingen, Augusto Castelli Von [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Introduction: Neuroendocrine Neoplasia (Nne) Is A Heterogeneous Group Of Tumors With Distinct Morphological And Biological Manifestations. The Liver Is The Main An Organ Affected By Its Metastases. On The Other Hand, Primary Hepatic Disease Is Extremely Rare, With Few Cases Reported In The Literature. Goal: Illustrate The Forms Of Common And Uncommon Presentations Of Primary Hepatic Nne Epidemiological Data, Clinical Data, Classification Pathology And Diagnosis Of Nne. Results: Four Cases Of Nne Were Selected And 15 Cases Of Secondary Hepatic Nne. The Main Way Of Primary Presentation Was Of Heterogeneous Mass, Predominantly With Hypervascular, With Necrotic Areas. In Cases Of Secondary Nne, The Main Pattern Of Imaging Was Composed Of Multiple Nodules With Hypervascular Enhancement Sparse By Both Lobes. However, Other Can Also Be Seen In Hepatic Nne, Such As With A Cystic Component, Or With Calcifications Of Permeation. Discussion: The Diagnosis Of Liver Involvement By This Disease Is A Challenge For The Radio
- ItemSomente MetadadadosPresent and Future of the Radio-Molecules used in Detection of Sentinel Lymphatic Nodes(Colegio Farmaceuticos Provincia De Buenos Aires, 2009-03-01) Fernandez Nunez, Eutimio Gustavo; Faintuch, Bluma Linkowski; Oliveira, Relma Tavares de; Wiecek, Danielle Pereira; Teodoro, Rodrigo; Oliveira Filho, Renato Santos de [UNIFESP]; Inst Energet & Nucl Res; Salvador Allende Hosp; Universidade Federal de São Paulo (UNIFESP)Detection of the sentinel lymph node has become a mainstay of certain surgical interventions for cancer. In this sense, physiological and biochemical properties of the molecules employed for early detection of such metastasis have become relevant in many specialties. Traditionally the main characteristics considered for such radiopharmaceuticals are particle size and surface features. In addition, design of radiolabeled molecules has to take in account anatomy of the vascular lymphatic epithelium and its interaction with such agents. The aim is to create more specific and effective drugs, thus precisely shaping and eventually remodelling the surgical strategy for a given patient. Advances in diagnostic imaging open perspectives for additional categories of agents, endowed with different physico-chemical features as required by positron-emission tomography and other sophisticated procedures. This review covers the molecules used in sentinel node finding as well as some related topics, which can help to understand their action mechanism and failures.
- ItemSomente MetadadadosPrognostic factors and outcomes for osteosarcoma: An international collaboration(Elsevier B.V., 2009-09-01) Pakos, Emilios E.; Nearchou, Andreas D.; Grimer, Robert J.; Koumoullis, Haris D.; Abudu, Adesegun; Bramer, Jos A. M.; Jeys, Lee M.; Franchi, Alessandro; Scoccianti, Guido; Campanacci, Domenico; Capanna, Rodolfio; Aparicio, Jorge; Tabone, Marie-Dominique; Holzer, Gerold; Abdolvahab, Fashid; Funovics, Philipp; Dominkus, Martin; Ilhan, Inci; Berrak, Su G.; Patino-Garcia, Ana; Sierrasesumaga, Luis; San-Julian, Mikel; Garraus, Moira; Petrili, Antonio Sergio [UNIFESP]; Garcia Filho, Reynaldo Jesus [UNIFESP]; Pacheco Donato Macedo, Carla Renata [UNIFESP]; Seixas Alves, Maria Teresa de [UNIFESP]; Seiwerth, Sven; Nagarajan, Rajaram; Cripe, Timothy P.; Ioannidis, John P. A.; Univ Ioannina; Royal Orthopaed Hosp; Univ Florence; Hosp Univ La Fe; Hop Enfants Armand Trousseau; Med Univ Vienna; Ankara Oncol Hosp; Marmara Univ; Univ Navarra; Univ Navarra Clin; Universidade Federal de São Paulo (UNIFESP); Med Fac Zagreb; Cincinnati Childrens Hosp; Tufts UnivWe aimed to evaluate the prognostic significance of traditional clinical predictors in osteosarcoma through an international collaboration of 10 teams of investigators (2680 patients) who participated. in multivariate models the mortality risk increased with older age, presence of metastatic disease at diagnosis, development of local recurrence when the patient was first seen, use of amputation instead of limb salvage/wide resection, employment of unusual treatments, use of chemotherapeutic regimens other than anthracycline and platinum and use of methotrexate. it was also influenced by the site of the tumour. the risk of metastasis increased when metastatic disease was present at the time the patient was first seen and also increased with use of amputation or unusual treatment combinations or chemotherapy regimens not including anthracycline and platinum. Local recurrence risk was higher in older patients, in those who had local recurrence when first seen and when no anthracycline and platinum were used in chemotherapy. Results were similar when limited to patients seen after 1990 and treated with surgery plus combination chemotherapy. This large-scale international collaboration identifies strong predictors of major clinical outcomes in osteosarcoma. (C) 2009 Published by Elsevier B.V.
- ItemSomente MetadadadosUpdate on Thin Melanoma: Outcome of an International Workshop(Lippincott Williams & Wilkins, 2016) Mihic-Probst, Daniela; Shea, Chris; Duncan, Lyn; de la Fouchardiere, Arnaud; Landman, Gilles [UNIFESP]; Landsberg, Jennifer; ven den Oord, Joost; Lowe, Lori; Cook, Martin G.; Yun, Sook Jung; Clarke, Loren; Messina, Jane; Elder, David E.; Barnhill, Raymond L.The following communication summarizes the proceedings of a 1-day Workshop of the International Melanoma Pathology Study Group, which was devoted to thin melanoma. The definitions and histologic criteria for thin melanoma were reviewed. The principal differential diagnostic problems mentioned included the distinction of thin melanoma from nevi, especially from nevi of special site, irritated nevi, inflamed and regressing nevi, and dysplastic nevi. Histologic criteria for this analysis were discussed and the importance of clinico-pathologic correlation, especially in acral sites, was emphasized. Criteria for the minimal definition of invasion were also discussed. In addition, a new technique of m-RNA expression profiling with 14 genes was presented and facilitated the distinction of thin melanomas from nevus in histologically obvious cases. However, for particular nevi, it was not obvious why the results indicated a malignant lesion. Despite many molecular and other ancillary investigations, Breslow thickness remains the most important prognostic factor in thin melanoma. The prognostic significance of radial (horizontal) and vertical growth phases, Clark level, regression, and mitotic rate were also discussed. Because of the increasing frequency of thin melanomas, there is a great need to develop more refined predictors of thin melanomas with worse clinical outcome.
- ItemSomente MetadadadosUpdate on Thin Melanoma: Outcome of an International Workshop(Lippincott Williams & Wilkins, 2016) Mihic-Probst, Daniela; Shea, Chris; Duncan, Lyn; de la Fouchardiere, Arnaud; Landman, Gilles [UNIFESP]; Landsberg, Jennifer; ven den Oord, Joost; Lowe, Lori; Cook, Martin G.; Yun, Sook Jung; Clarke, Loren; Messina, Jane; Elder, David E.; Barnhill, Raymond L.; Universidade Federal de São Paulo (UNIFESP)The following communication summarizes the proceedings of a 1-day Workshop of the International Melanoma Pathology Study Group, which was devoted to thin melanoma. The definitions and histologic criteria for thin melanoma were reviewed. The principal differential diagnostic problems mentioned included the distinction of thin melanoma from nevi, especially from nevi of special site, irritated nevi, inflamed and regressing nevi, and dysplastic nevi. Histologic criteria for this analysis were discussed and the importance of clinico-pathologic correlation, especially in acral sites, was emphasized. Criteria for the minimal definition of invasion were also discussed. In addition, a new technique of m-RNA expression profiling with 14 genes was presented and facilitated the distinction of thin melanomas from nevus in histologically obvious cases. However, for particular nevi, it was not obvious why the results indicated a malignant lesion. Despite many molecular and other ancillary investigations, Breslow thickness remains the most important prognostic factor in thin melanoma. The prognostic significance of radial (horizontal) and vertical growth phases, Clark level, regression, and mitotic rate were also discussed. Because of the increasing frequency of thin melanomas, there is a great need to develop more refined predictors of thin melanomas with worse clinical outcome.