Navegando por Palavras-chave "Metallo-beta-lactamase"
Agora exibindo 1 - 6 de 6
Resultados por página
Opções de Ordenação
- ItemSomente MetadadadosComparison of phenotypic tests for the detection of metallo-beta-lactamases in clinical isolates of Pseudomonas aeruginosa(Ediciones Doyma S A, 2014-12-01) Lucena, Andrea; Dalla Costa, Libera M.; Nogueira, Keite da Silva; Matos, Adriana P. [UNIFESP]; Gales, Ana C. [UNIFESP]; Raboni, Sonia M.; Univ Fed Parana; Fac & Inst Pesquisa Pele Pequeno Principe; Universidade Federal de São Paulo (UNIFESP)Metallo-beta-lactamase (MBL)-producing gram-negative bacteria are an increasing public health concern worldwide. Screening tests for the rapid and specific identification of these pathogens are essential, and should be included among routine diagnostics in laboratories. This study aimed to determine the MBL frequency among carbapenem-resistant Pseudomonas aeruginosa isolates, and to evaluate the accuracy of different tests in screening for MBL production. From January 2001 to December 2008, a total of 142 imipenem-non-susceptible P. aeruginosa strains were isolated from distinct clinical samples from hospitalized patients. These isolates were examined by PCR, MBL E-test, double-disk synergy test (DDST), and combined disk (CD) test. the minimal inhibitory concentration (MIC; mu g/mL) was determined by agar dilution, and pulsed field gel electrophoresis (PFGE) was performed on all samples. Sequencing was performed to confirm and define the MBL variant and subtype. Using PCR and DNA sequence analysis, 93 strains were confirmed positive for MBLs, 91 strains for the blaSPM-1 gene, 1 strain for the blaIMP-1 gene, and 1 strain for the blaIMP-16 gene. PFGE displayed a clonal pattern. the sensitivities, specificities, positive and negative predictive values were evaluated for all tests. the DDST assay (CAZ-MPA) was the optimal method for screening MBL production in P. aeruginosa strains. However, the results of the CD assay (IMP/EDTA) showed close agreement with those of the DDST. in addition, the CD assay allowed a more objective interpretation and did not require the use of a toxic substance. (C) 2013 Elsevier Espana, S.L.U. and Sociedad Espanola de Enfermedades Infecciosas y Microbiologia Clinica. All rights reserved.
- ItemSomente MetadadadosDetection of bla(VIM-7) in an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST1284 in Brazil(Elsevier Science Inc, 2017) de Paula, Suelen Balero; Cayo, Rodrigo [UNIFESP]; Streling, Ana Paula [UNIFESP]; Nodari, Carolina Silva [UNIFESP]; Matos, Adriana Pereira [UNIFESP]; Eches Perugini, Marcia Regina; Gales, Ana Cristina [UNIFESP]; Carrara-Marroni, Floristher Elaine; Yamada-Ogatta, Sueli F.We described for the first time an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST1284 carrying a plasmid-mediated bla(VIM-7) in Brazil. The bla(VIM-7) was harbored by an integron that also carried aacA4 and bla(OXA-46). Multiple virulence factors were also detected. (C) 2017 Elsevier Inc. All rights reserved.
- ItemAcesso aberto (Open Access)Identification of Sao Paulo metallo-beta-lactamase-1-producing Pseudomonas aeruginosa in the Central-West region of Brazil: a case study(Soc Brasileira Medicina Tropical, 2017) Maciel, Wirlaine Glauce; da Silva, Kesia Esther; Bortolotto Bampi, Jose Victor; dos Santos Bet, Graciela Mendonca; Ramos, Ana Carolina [UNIFESP]; Gales, Ana Cristina [UNIFESP]; Simionatto, SimoneMetallo-beta-lactamase production is an important mechanism for carbapenem resistance of Pseudomonas aeruginosa, which represents an emerging public health challenge. We report the case of a patient admitted to an intensive care unit, with sepsis caused by multidrug-resistant Sao Paulo Metallo-beta-lactamase-1-producing P. aeruginosa. This is the first case of infection by this pathogenic strain in the State of Mato Grosso do Sul, Brazil. Thus, infection control measures are required for preventing future spread and outbreaks.
- ItemAcesso aberto (Open Access)Metalo-beta-lactamases(Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia, 2006-04-01) Mendes, Rodrigo Elisandro [UNIFESP]; Castanheira, Mariana [UNIFESP]; Pignatari, Antonio Carlos Campos [UNIFESP]; Gales, Ana Cristina [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Laboratório ALERTA Laboratório Especial de Microbiologia ClínicaIncrease isolation of Gram-negative bacilli resistant to broad-spectrum cephalosporin has been observed during the last few years, thus determining the use of more potent beta-lactams, such as carbapenems. The use of these antimicrobial agents may lead to the emergence of carbapenem resistant Gram-negative bacilli in the nosocomial environment. Carbapenem resistance may be due to the production of Ambler class D beta-lactamase or Ambler class B beta-lactamase, also called metallo-beta-lactamase (MbetaL). Apart from the monobactam aztreonam, this class of enzyme virtually hydrolyze all the commercially available beta-lactams. Since 90s, several clinical important nosocomial microorganisms, including members of Enterobacteriaceae family, Pseudomonas spp. and Acinetobacter spp., have been found to produce MbetaLs enzymes. When these carbapenem non-susceptible strains are found, they may be submitted to phenotypic MbetaL detection test in the microbiology laboratory in order to help infection control practioners and prevent the MbetaL gene dissemination, once these genes are embedded in mobile genetic elements, which can spread rapidly to other Gram-negative species.
- ItemSomente MetadadadosMicrobiological and epidemiological characterization of imipenem-resistant Pseudomonas aeruginosa strains from a Brazilian tertiary hospital: Report from the SENTRY Antimicrobial Surveillance Program(Esift Srl, 2006-10-01) Ribeiro, Julival; Mendes, Rodrigo Elisandro [UNIFESP]; Domingos, R.; Franca, E.; Silbert, Suzane [UNIFESP]; Jones, Ronald N.; Sader, Helio Silva [UNIFESP]; Hosp Base Distrito Fed; Universidade Federal de São Paulo (UNIFESP); JMI LabsOBJECTIVES: To evaluate the antimicrobial susceptibility profile, the genetic similarity, and the mechanisms of carbapenem resistance among imipenem-resistant Pseudomonas aeruginosa isolates collected from a Brazilian tertiary teaching hospital.METHODS: Seventy-eight consecutive samples of P. aeruginosa were evaluated during 2000 and 2001. The antimicrobial susceptibility was evaluated by reference broth microdilution methods and the imipenem-resistant isolates were screened for metallo-p-lactamase (MPL) production throughout disc approximation test and MOL Etest (R) strips and isolates with positive screen test result were submitted to PCR assays using primers bla(IMP-1), bla(VIM-1), bla(VIM-2) e bla(SPM-1). The genetic similarity of MOL-producing strains was evaluated by automated ribotyping for epidemiological typing purpose.RESULTS: Resistance rates were high to the majority of antimicrobial agents tested except polymyxin B, which inhibited all samples at the Clinical and Laboratory Standards Institute breakpoint (<= 2 mu g/ml). Twenty-nine (37.2%) isolates were resistant to imipenem and these isolates showed great genomic variability. MPL production was demonstrated in two imipenem-resistant isolates, which were detected using bla(SPM-1) and bla(IMP-2)-specific primers. Sequence analysis revealed the presence of bla(SPM-1) and a novel biamp-type gene, bla(IMP)-typeCONCLUSION: The results of this study showed high resistance rates to the majority of antimicrobial agents among P. aeruginosa samples. High imipenem resistance rates were probably due to continuous selection of resistant mutants. The production of MOL did not represent a frequent mechanism of carbapenem resistance in this medical center; but a novel MPL was identified. Continued antimicrobial surveillance and infection control measures should be emphasized to minimize the emergence and dissemination of antimicrobial resistance.
- ItemSomente MetadadadosNosocomial infections with metallo-beta-lactamase-producing Pseudomonas aeruginosa: molecular epidemiology, risk factors, clinical features and outcomes(W B Saunders Co Ltd, 2014-08-01) Lucena, A.; Dalla Costa, L. M.; Nogueira, K. S.; Matos, A. P. [UNIFESP]; Gales, A. C. [UNIFESP]; Paganini, M. C.; Castro, M. E. S.; Raboni, S. M.; Univ Fed Parana; Fac & Inst Pesquisa Pele Pequeno Principe; Universidade Federal de São Paulo (UNIFESP)Background: Metallo-beta-lactamases (MBLs) have emerged as one of the most important bacterial resistance mechanisms because of their ability to hydrolyse virtually all beta-lactam agents. MBL-producing Pseudomonas aeruginosa (MBL-PA) are an important cause of nosoconnial infections, particularly in intensive care units (ICUs), where they are associated with serious infections and present a significant clinical risk.Aim: To assess the molecular epidemiology, risk factors and outcomes of nosocomial infections caused by MBL-PA in a teaching hospital in Southern Brazil.Methods: From January 2001 to December 2008, 142 carbapenem-resistant P. aeruginosa strains were isolated from distinct clinical samples from hospitalized patients. These isolates were screened for MBLs, and underwent polymerase chain reaction, sequencing and pulsed-field gel electrophoresis (PFGE). Patients infected with carbapenem-resistant MBL-PA were considered as cases, and patients infected with non-MBL-PA were considered as controls.Findings: Eighty-four of 142 patients with positive carbapenem-resistant P. aeruginosa cultures met the criteria of the Centers for Disease Control and Prevention for infection. Fifty-eight patients were infected with MBL-PA (69%) and 26 patients were infected with non-MBL-PA (31%). Multi-variate analysis revealed that ICU stay [P = 0.003, odds ratio (OR) 4.01, 95% confidence interval (CI) 1.15-14.01] and urinary tract infection (P = 0.001, OR 9.67, 95% CI 1.72-54.48) were important risk factors for MBL-PA infection. Patients infected with MBL-PA showed faster onset of infection (P = 0.002) and faster progression to death (P = 0.04).Conclusions: These results showed the severity of MBL-PA infections, and demonstrated the urgent need for strategies to improve infection control measures to prevent an increase in these nosocomial infections. (C) 2014 the Healthcare Infection Society. Published by Elsevier B.V. All rights reserved.