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- ItemSomente MetadadadosComparação biomecânica das técnicas de sutura vertical e cruzada, em configurações de fileira única e dupla, para o tratamento da lesão meniscal medial em alça de balde(Universidade Federal de São Paulo (UNIFESP), 2020-07-01) Nakama, Gilberto Yoshinobu [UNIFESP]; Cohen, Moises [UNIFESP]; Universidade Federal de São PauloBackground: Due to a variety of suturing techniques for bucket-handle meniscal repair, it is important to assess which suturing technique best restores native biomechanics. Purpose: To biomechanically compare vertical mattress and cross-stitch suture techniques, in single- and doublerow configurations, in their ability to restore native knee kinematics in a bucket-handle medial meniscus tear model. Methods: Ten matched pairs of human cadaver knees were randomly assigned to vertical mattress (n = 10) or cross-stitch (n = 10) repair groups. Each knee underwent four consecutive testing conditions: (1) intact, (2) displaced bucket-handle tear, (3) single-row suture configuration on the femoral meniscus surface, and (4) double-row suture configuration (repair of both femoral and tibial meniscus surfaces). Knees were loaded with a 1000 N axial compressive force at 0°, 30°, 60°, 90° and 120° of flexion for each condition. Resultant medial compartment contact area, average contact pressure, and peak contact pressure data were recorded. Results: Intact state contact area was not restored at 0° (P = 0.027) for the vertical double-row configuration and at 0° (P = 0.032), 60° (P < 0.001) and 90° (P = 0.007) of flexion for the cross-stitch double-row configuration. No significant differences were found in the average contact pressure and peak contact pressure between the intact state and the vertical mattress and cross-stitch repairs with either single- and double-row configurations at any flexion angles. When comparing the vertical and crossstich repairs across all flexion angles, no significant differences were observed in single-row configurations, but in double-row configurations, cross-stitch repair resulted in a significantly decreased contact area, average contact pressure and peak contact pressure (all P < 0.001). Conclusion: XVII Single- and double-row configurations of both vertical mattress and crossstitch inside-out meniscal repair techniques restored native tibiofemoral pressure after a medial meniscus bucket-handle tear at all assessed knee flexion angles. Despite decreased contact area using a double-row configuration, mainly related to the cross-stitch repair, in comparison to the intact state, the cross-stitch double-row repair led to decreased pressure in comparison to the vertical double-row repair. These findings are only applicable at the time of the surgery.
- ItemAcesso aberto (Open Access)A Correlação entre diferentes temperaturas e períodos de preservação sobre as fibras colágenas de meniscos de coelhos(Universidade Federal de São Paulo (UNIFESP), 2006) Paiva, Vanessa Carla [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objetivo: Quantificar as fibras colágenas de meniscos de coelhos submetidos à temperaturas e períodos de preservação variáveis. Métodos: Retirou-se assepticamente 120 meniscos (mediais e laterais) de 30 coelhos (Nova Zelândia), machos, idade entre 6/8 meses, peso médio de 3250g. Os meniscos foram congelados, de dois até trinta dias, a 7,2°Celsius negativos (n=60) e 73°Celsius negativos (n=60). A cada dois dias, de cada temperatura, foram descongelados quatro meniscos que foram encaminhados para o estudo histológico. A descrição morfológica foi feita com os cortes corados por HE, e para o estudo morfométrico os cortes foram corados pelo método de Picrosirius e posteriormente submetidos à polarização. A quntificação de área ocupada pelas fibras colágenas em cada lâmina foi calculada por subtração de imagens à partir do programa de software Image Pro Plus. Resultados: Na análise descritiva das médias percentuais de colágeno foram observadas diferenças maiores e estatisticamente significantes (p<0,001) à temperatura de 73ºCelsius negativos. A análise comparativa das médias percentuais de fibras colágenas ao longo dos períodos de preservação, embora com diferença de valores, apresentaram uma curva simétrica de variação. Também obervou-se que à partir do 14ºdia, em ambas temperaturas, a variação na porcentagem de fibras colágenas em relação aos demais períodos, não apresenta significância estatística, contudo a temperatura de 73ºCelsius negativos mostrou-se superior à 7,2ºCelsius negativos na preservação de fibras colágenas. A análise de regressão polinomial mostrou que a variação das médias percentuais de fibras colágenas é um polinômio de terceiro grau, sugerindo outras variáveis implicadas na preservação do colágeno. Conclusão: A temperatura de 73ºCelsius negativos mostrou médias percentuais de colágeno superiores em todos os períodos observados, porém à partir do 14º até o 30º dia de congelamento, a quantidade de fibras colágenas permanece estável em ambas temperaturas.
- ItemAcesso aberto (Open Access)O papel do adesivo de fibrina e da sutura na fixação do transplante de menisco preservado por ultracongelamento em coelhos(Sociedade Brasileira de Ortopedia e Traumatologia, 2009-10-01) Reckers, Leandro José [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Raymundo, José Luiz Pozo; Granata Júnior, Geraldo Sérgio De Mello [UNIFESP]; Moreira, Márcia Bento [UNIFESP]; Paiva, Vanessa Carla [UNIFESP]; Fagundes, Anna Luiza Negrini; Cohen, Moises [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal de Pelotas Departamento de Ortopedia; Universidade de Santo AmaroOBJECTIVE: To evaluate the ability of fibrin adhesive in promoting the meniscus fixation within two, four and eight weeks compared to the conventional soft-tissue suture technique. MATERIALS AND METHODS: 36 right medial menisci of rabbits preserved at negative 73° Celsius for 30 days were transplanted to animals of the same sample and fixed with soft-tissue suture or fibrin glue. After 2, 4 or 8 weeks, the appearance of the menisci and the quality of fixation were macroscopically checked and evaluated by a scoring system. The findings were subjected to the statistical study of variance analysis (p < 0.05%). RESULTS: The deep-frozen meniscus preservation maintained the integrity of the meniscus transplant, and, macroscopically, there was no significant reduction of the length of the meniscus in all post-transplant periods (p = 0.015). The menisci fixed with fibrin showed slight changes in color and surface roughness. There were no signs of rejection or infection in both groups. Suture fixation scoring was superior (p = 0.015) in all periods (80% of total fixation) as compared to the setting promoted by fibrin (20% of total fixation). CONCLUSION: The homologous transplantation of the meniscus of rabbits experienced various degrees of integration to the knee according to the fixation method; the surgical soft tissues suturing technique was shown to be superior in the evaluation of scores compared to the fixation with fibrin adhesive.
- ItemSomente MetadadadosRelação entre o risco rotura do ligmento cruzado anterior e inclinação tibial posterior em atletas profissionais de futebol(Universidade Federal de São Paulo (UNIFESP), 2019-11-01) Ikawa, Marcos Hiroyuki [UNIFESP]; Arliani, Gustavo Goncalves [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Introduction: Several anatomical parameters and external factors have been related to increased risk of anterior cruciate ligament injury. However, published studies have conflicting results. Objectives: The aim of this study was to evaluate if the is a relationship between the risk of anterior cruciate ligament rupture and the posterior tibial slope. The secundary objective was to evaluate if the is a relationship between posterior meniscal slope, delta-posterior tibial slope, delta-posterior mesnical slope, intercondylar notch width and intercondylar notch width index. Method: A retrospective case-control study was conducted on samples of professional male soccer players, using knee magnetic resonance imaging, divided into case groups, those with clinical or radiological diagnosis of anterior cruciate ligament rupture; and control group, those without signs of ligament rupture. Orthopedic measurements were performed based on previus published. Results: Lateral and medial posterior tibal slopes, lateral and medial posterior meniscal slopes, delta-posterior tibial and meniscal slopes, intercondylar notch width and intercondylar notch width index presented statistically significant difference between the groups (p < 0,05). Through multivariate logistic regression analysis, lateral and medial posterior meniscal slope were predictor for increased risk of anterior cruciate ligament rupture. Conclusion: Lateral and medial posterior tibal slope, lateral and medial posterior meniscal slope, delta-posterior tibial and meniscal slope, intercondylar notch width and intercondylar notch width index have statistically significant relationship with higher risk of anterior cruciate ligament rupture in professional male soccer players. Lateral and medial posterior meniscal slopes are predictors of ligament injury.