Navegando por Palavras-chave "Male reproductive tract"
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- ItemSomente MetadadadosCloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract(Oxford Univ Press, 2008-02-01) Patrão, Marilia Tavares Coutinho da Costa [UNIFESP]; Queiroz, Daniel Barboza Cava [UNIFESP]; Grossman, Gail; Petrusz, Peter; Lazari, Maria de Fatima Magalhaes [UNIFESP]; Avellar, Maria Christina Werneck [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ N CarolinaThis study reports the genomic organization of the rhesus alpha(1A)-adrenoceptor gene (ADRA1A). Full-length cloning of rhesus ADRA1A splice variants was achieved by combining PCR screening of a seminal vesicle cDNA library and 5'-RACE assays with total RNA from seminal vesicle. the classical ADRA1A mRNA (ADRA1A_nu 1) and six full-length ADRA1A splice variants were identified representing transcripts that code for functional (ADRA1A_nu 1, ADRA1A_nu 2a, ADRA1A_nu 3a, ADRA1A_nu 3d, ADRA1A_nu 3e) and truncated (ADRA1A_nu 2c and ADRA1A_nu 3c) receptor isoforms. Comparative analysis of the deduced amino acid sequence indicated that rhesus ADRA1A_i1 isoform (corresponding to the ADRA1A_nu 1 transcript) shares high identity to the amino acid sequence present in the classical alpha(1A)-adrenoceptor from human and other mammalian species. Partial nucleotide sequences for rhesus alpha(1B)-(ADRA1B) and alpha(1D)-adrenoceptor (ADRA1D) transcripts were also characterized. RT-PCR studies indicated differential distribution of all ADRA1A-related splice variants as well as ADRA1B and ADRA1D mRNAs, in tissues from rhesus and human male reproductive tract. Immunohistochemistry revealed alpha(1A)-adrenoceptor (ADRA1A_ i1) immunostaining in smooth muscle cells and epithelial cells of rhesus efferent ductules, epididymis and seminal vesicle. Taken together the present results demonstrate that the complexity of the splicing mechanisms involved in the regulation of the ADRA1A gene is not restricted to human and is a common characteristic among Old World monkeys.
- ItemSomente MetadadadosEffects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductules(Soc Study Reproduction, 2008-09-01) Yasuhara, Fabiana [UNIFESP]; Gomes, Gisele Renata de Oliveira [UNIFESP]; Siu, Erica Rosanna [UNIFESP]; Suenaga, Claudia Igushi [UNIFESP]; Marostica, Elisabeth [UNIFESP]; Porto, Catarina Segreti [UNIFESP]; Lazari, Maria de Fatima Magalhaes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The efferent ductules express the highest amount of estrogen receptors ESR1 (ERalpha) and ESR2 (ERbeta) within the male reproductive tract. Treatment of rats with the antiestrogen fulvestrant (ICI 182,780) causes inhibition of fluid reabsorption in the efferent ductules, leading to seminiferous tubule atrophy and infertility. To provide a more comprehensive knowledge about the molecular targets for estrogen in the rat efferent ductules, we investigated the effects of ICI 182,780 treatment on gene expression using a microarray approach. Treatment with ICI 182,780 increased or reduced at least 2-fold the expression of 263 and 98 genes, respectively. Not surprisingly, several genes that encode ion channels and macromolecule transporters were affected. Interestingly, treatment with ICI 182,780 markedly altered the expression of genes related to extracellular matrix organization. Matrix metalloproteinase 7 (Mmp7), osteopontin (Spp1), and neuronal pentraxin 1 (Nptx1) were among the most altered genes in this category. Upregulation of Mmp7 and Spp1 and downregulation of Nptx1 were validated by Northern blot. Increase in Mmp7 expression was further confirmed by immunohistochemistry and probably accounted for the decrease in collagen content observed in the efferent ductules of ICI 182,780-treated animals. Downregulation of Nptx1 probably contributed to the extracellular matrix changes and decreased amyloid deposition in the efferent ductules of ICI 182,780-treated animals. Identification of new molecular targets for estrogen action may help elucidate the regulatory role of this hormone in the male reproductive tract.
- ItemSomente MetadadadosIn Vivo Treatments with Fulvestrant and Anastrozole Differentially Affect Gene Expression in the Rat Efferent Ductules(Soc Study Reproduction, 2011-01-01) Gomes, Gisele Renata de Oliveira [UNIFESP]; Yasuhara, Fabiana [UNIFESP]; Siu, Erica Rosanna [UNIFESP]; Fernandes, Sheilla Alessandra Ferreira [UNIFESP]; Avellar, Maria Christina Werneck [UNIFESP]; Lazari, Maria de Fatima Magalhaes [UNIFESP]; Porto, Catarina Segreti [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Inst Nacl Farmacol & Biol MolEstrogen plays a key role in maintaining the morphology and function of the efferent ductules We previously demonstrated that the antiestrogen fulvestrant markedly affected gene expression in the rat efferent ductules the mechanism of fulvestrant action to modulate gene expression may involve not only the blockade of ESR1 and ESR2 estrogen receptors, but also the activation of ESR1 and ESR2 when the receptors are tethered to AP 1 or SP1 transcription factors, or the activation of the G protein-coupled estrogen receptor 1 We therefore compared the effects of two strategies to interfere with estrogen action in the rat efferent ductules treatment with fulvestrant or with the aromatase inhibitor anastrozole Whereas fulvestrant markedly increased Mmp7 and Spp1, and reduced Npfx1 mRNA levels, no changes were observed with anastrozole Fulvestrant caused changes in epithelial morphology that were not seen with anastrozole Fulvestrant shifted MMP7 immunolocalization in the epithelial cells from the supranuclear to the apical region, this effect was less pronounced with anastrozole in vitro studies of S-35-methiomne incorporation showed that protein release was increased, whereas tissue protein content in the efferent ductules of fulvestrant-treated rats was decreased Although fulvestrant markedly affected gene expression, no changes were observed on AP 1 and SP1 DNA-binding activity the blockade of ESRs seems to be the major reason explaining the differences between both treatments At least some of the effects of fulvestrant appear to result from compensatory mechanisms activated by the dramatic changes caused by ESR1 blockade
- ItemSomente MetadadadosMuscarinic Acetylcholine Receptor Subtypes in the Male Reproductive Tract(Humana Press Inc, 2010-01-01) Avellar, Maria Christina Werneck [UNIFESP]; Siu, Erica Rosanna [UNIFESP]; Yasuhara, Fabiana [UNIFESP]; Marostica, Elisabeth; Porto, Catarina Segreti [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal Fluminense (UFF)In mammals, at least five different muscarinic acetylcholine receptor subtypes (mAChRs; M(1)-M(5)) are known to be widely expressed and distributed in different tissues from different species. They mediate distinct physiological functions according to their location and receptor subtype. Multiple events are associated with the regulation of intracellular signaling by mAChRs, and a coordinated balance of the molecular mechanisms governing receptor signaling, desensitization, resensitization, and mitogenic signaling is known to occur in various cell types. Most of the actions of acetylcholine (ACh) in the male reproductive tract are induced by its effects on mAChRs, but the role of specific mAChR subtypes on male reproductive function and fertility are still not well understood. the rat efferent ductules and epididymis are androgen-dependent tissues of the male reproductive tract, with important roles in the process to form a viable and fertile sperm. in the present study, aspects of the expression, localization, and potential function of mAChR subtypes in rat efferent ductules and epididymis are reviewed. Furthermore, evidences for the implication of mAChRs in the regulation of protein synthesis and secretion in these tissues are presented. Taken together, the studies contribute to our understanding about physiological aspects of mAChR and mechanisms by which the cholinergic system affects male reproduction.