Navegando por Palavras-chave "Lymphocyte proliferation"

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    Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro
    (Assoc Bras Divulg Cientifica, 2017) Almeida, V. G.; Avelar-Freitas, B. A.; Santos, M. G.; Costa, L. A.; Silva, T. J.; Pereira, W. F.; Amorim, M. L. L.; Grael, C. F. F.; Gregorio, Luiz Elídio [UNIFESP]; Rocha-Vieira, E.; Brito-Melo, G. E. A.
    Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic (ETA) and acetate (ACE) extracts showed cytotoxic effects. The aqueous extract (AQU) was not cytotoxic. Peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin and treated with AQU (100 mu g/mL) showed reduced interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha expression. AQU also inhibited lymphocyte proliferative response after nonspecific stimulation with phytohemagglutinin. The aqueous extract was analyzed by liquid chromatography coupled with photodiode array detection and mass spectrometry. Quinic acid and its derivatives 5-caffeoylquinic acid and 3,5dicaffeoylquinic acid, as well as the flavonoids luteolin and luteolin dihexoside, were detected. All these compounds are known to exhibit anti-inflammatory activity. Taken together, these findings demonstrate that P. brasiliensis aqueous extract can inhibit the pro-inflammatory cytokine production and proliferative response of lymphocytes. These effects may be related to the presence of chemical substances with anti-inflammatory actions previously reported in scientific literature.
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    Lymphocyte proliferation stimulated by activated human macrophages treated with Canova
    (Elsevier B.V., 2009-01-01) Burbano, Rommel Rodriguez; Leal, Mariana Ferreira [UNIFESP]; Costa, Joana Borges da; Bahia, Marcelo de Oliveira; Lima de Lima, Patricia Danielle; Khayat, Andre Salim; Seligman, Igor Chamon; Assumpcao, Paulo Plmentel de; Buchi, Dorly de Freitas; Cardoso Smith, Marilia de Arruda [UNIFESP]; Fed Univ Para; Universidade Federal de São Paulo (UNIFESP); Univ Fed Parana
    Introduction: Canova (CA) is a homeopathic medication with immunomodulatory properties, recommended for patients with a depressed immune system. CA has been reported to increase in leukocyte numbers, cellular differentiation and reduction in tumor size.Aim and method. Since CA may stimulate lymphocyte differentiation, proliferation, and/or survival, the aim of the present study was to compare the mitotic index (MI) of phytohemagglutinin-stimulated human lymphocytes cultured in a medium supplemented with human macrophages activated by CA, with lymphocytes cultured in a medium without CA-treated macrophages.Results: in this study, the MI of lymphocyte cultured received the medium containing CA-stimulated macrophages showed a higher proliferation index (p < 0.01) than the lymphocytes cultured in a medium without CA-treated macrophages. Our results suggest that CA treatment, in addition to activating macrophages, indirectly induces lymphocyte proliferation and has potential as a new adjuvant therapeutic approach. Homeopathy (2009) 98, 45-48.
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    Moderate exercise increases the metabolism and immune function of lymphocytes in rats
    (Springer, 2013-05-01) Navarro, Francisco; Nova Bacurau, Aline Villa; Pereira, Guilherme Borges; Araujo, Ronaldo Carvalho [UNIFESP]; Almeida, Sandro Soares [UNIFESP]; Moraes, Milton Rocha [UNIFESP]; Uchida, Marco Carlos; Pereira Costa Rosa, Luis Fernando Bicudo; Navalta, James; Prestes, Jonato; Pereira Bacurau, Reury Frank; Univ Fed Maranhao; Universidade de São Paulo (USP); Universidade Federal de São Carlos (UFSCar); Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Campinas (UNICAMP); Univ Nevada; Univ Catolica Brasilia
    Exercise modulates both glucose and glutamine metabolism which influences lymphocyte function. We investigated the influence of chronic moderate exercise on glucose and glutamine metabolism in lymphocytes, the associated influence on proliferation, and cytokine and immunoglobulin production. Male Wistar rats (8 weeks old) were placed in an exercise training group (N = 15, 1 h day(-1) at 60 % VO2max, 5 days week(-1)) for 8 weeks of exercise, or a sedentary control group. Twenty-four hours following the final training session, lymphocytes were separated, and the incorporation of [U-14C]-glucose, [U-14C]-glutamine, and [2-14C]-thymidine from the supernatant was measured. the activity of glucose-6-phosphate dehydrogenase, hexokinase, and glutaminase was measured. Lymphocytes were stimulated with ConA and LPS and incubated with the Mycobacterium bovis bacille Calmette-Guerin (BCG) vaccine and plasma IgG and IgE were measured. Glutamine metabolism increased in both T and B lymphocytes in the trained group. in the trained group, proliferative capacity increased T lymphocytes under ConA stimulation, and increased B lymphocytes with LPS. There was a significant increase in IL-2 production and decrease in IL-4 in the trained group compared with sedentary controls. IL-2R and TNFR increased in trained rats while IL-4R decreased and were more pronounced in T lymphocytes compared with B lymphocytes. in both lymphocyte subsets, exercise training significantly increased the expression of CD54+ and CD30+ cell markers. Exercise training increased plasma IgG compared with the sedentary group. in conclusion, moderate exercise training improves immune function and metabolism in T and B lymphocytes, reflecting an increased ability to respond to immune challenges.