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- ItemAcesso aberto (Open Access)Avaliação do papel da proteína anti-inflamatória Anexina A1 na regulação do Inflamassoma NLRP3 e nas infecções por Candida Albicans e Candida Auris: estudo in vitro e caracterização do perfil lipidômico(Universidade Federal de São Paulo, 2020-07-17) Sanches Junior, José Marcos; Gil, Cristiane Damas [UNIFESP]; http://lattes.cnpq.br/6047408996026135; http://lattes.cnpq.br/2853203620000520; Universidade Federal de São Paulo (UNIFESP)Objetivo: Avaliar o papel da AnxA1 na regulação do inflamassoma NLRP3 e na infecção por C. albicans e C. auris em macrófagos e neutrófilos isolados, bem como avaliar o perfil dos lipídios liberados por essas células após a ativação do NLRP3 e nas infecções por Candida spp. Metodologia: Camundongos machos C57BL/6 selvagens (WT) e nocautes para AnxA1 (AnxA1-/- ) receberam injeção intraperitoneal de solução de amido a 1,5% ou carragenina a 0,3% para obtenção de macrófagos e neutrófilos do lavado peritoneal, respectivamente. Essas células foram estimuladas com lipopolissacarídeo (LPS), (por 3 horas), seguida da administração dos agonistas de NLRP3, nigericina (1 hora) ou ATP (30 minutos). Também foi verificado a ação do peptídeo mimético da AnxA1 (Ac2-26) em neutrófilos ativados pelo NLRP3. Para verificar a resposta de neutrófilos em infecções fúngicas, bem como a relação da AnxA1 nestas condições, estas células foram cocultivadas com as cepas de C. albicans e C. auris por 4 horas. Resultados: Nos macrófagos evidenciou-se que a falta de AnxA1 exacerba a liberação de IL-1β após a indução da ativação do inflamassoma NLRP3, bem como acarreta mudanças no perfil lipídico, tendo as ceramidas como os principais lipídios mediadores. A expressão de NLRP3 foi maior em macrófagos AnxA1-/- tratados com nigericina e pontos de colocalização foram verificados entre o inflamassoma NLRP3 e AnxA1, evidenciando a participação dessa proteína na regulação do NLRP3. Em neutrófilos, a AnxA1 endógena demonstrou ser importante para a ativação do NLRP3, uma vez que a liberação de IL-1β foi inferior quando comparado aos WT. A adição do peptídeo Ac2-26 reduziu a liberação de IL-1β pela via de ativação do inflamassoma NLRP3 em neutrófilos WT, mas não reverteu a liberação desta citocina em neutrófilos AnxA1-/- . Potenciais biomarcadores lipídicos relacionados ao estresse e ativação celular, incluindo esfingolipídios específicos e glicerofosfolipídios, foram identificados no sobrenadante dos neutrófilos de ambos genótipos após ativação por nigericina. O tratamento com Ac2-26 aumentou a concentração de fosfatidilserinas e fosfocolinas oxidadas, biomarcadores lipídicos relacionados à via da resolução inflamatória. Neutrófilos AnxA1-/- cocultivados com C. albicans ou C. auris apresentam maior expressão de COX-2 e ativação das MAPKs em relação aos WT. A falta de AnxA1 promoveu diferença no perfil lipídico, tendo maior proeminência de glicerofosfolipídios, tanto em neutrófilos co-infectados quanto no grupo AnxA1-/- controle. Conclusão: A AnxA1 desempenha diferentes papeis na sinalização do inflamassoma NLRP3 com base em sua localização intra ou extracelular e tipo celular. Além disso, regula a ativação dos neutrófilos pela infecção por Candida spp., evidenciando seu potencial imunomodulador em doenças inflamatórias e infecciosas.
- ItemAcesso aberto (Open Access)Efeito da adição do LH durante o estímulo ovariano e maturação oocitária no perfil lipídico de oócitos murinos(Universidade Federal de São Paulo (UNIFESP), 2016-02-02) Oleinki, Talitha Dinardo [UNIFESP]; Fraietta, Renato [UNIFESP]; http://lattes.cnpq.br/1545035937368744; http://lattes.cnpq.br/7699135183138091; Universidade Federal de São Paulo (UNIFESP)Introduction: The addition of LH in assisted reproduction aiming to obtain a higher number of viable follicles for the treatment is still controversial. A good embryonic development depends on the follicular growth and proper oocyte maturation. Because of that, the association between alkaline comet assay and the study of oocyte lipidomics may help to understand the biological processes involved in oocyte maturation and the influence of hormonal protocol. This study may contribute in the future with effective hormonal protocols, improving the quality of oocytes and pregnancy rates. Objective: Evaluate the effect of adding LH on DNA integrity of cumulus cells and on lipid profile of murine oocytes. Methods: Female mice C57BL / 6J 23 to 28 days old received three different stimulation protocols intraperitoneally (i.p.): (i) only PMSG; (ii) PMSG plus hCG (iii) PMSG plus LHr. After 48 hours of administration, immature oocytes were collected and cultured for 24 hours in three different culture medium for maturation: (i) only FSH; (ii) FSH plus hCG (iii) FSH plus LHr. After this period, the oocytes which had the first polar body were frozen at -80oC until the analysis by electrospray ionization (ESI) and cumulus cells were subjected to alkaline comet technique to measure the integrity of DNA. The principal component analysis and discriminant analysis by least squares were performed and 35 ions with greater representation were identified by the variable importance in the projection. Results: There was no statistical difference in the maturation rate and DNA fragmentation of cumulus cells in different groups analyzed. The fingerprinting analysis of the lipid profile of oocytes matured in vitro identified twenty lipids. The hyper-represented lipids in the group stimulated with the addition of hCG and matured in culture medium only with FSH (HF group) are: phosphatidylethanolamine, polyketide (flavonoid), sterol, phosphatidylserine, polyketide (ansamycin), sphingomyelin and phosphatidylcholine; in the group stimulated and matured in the presence of hCG (HH group) phosphatidylglycerol, phosphatidylserine, sphingolipids and triacylglycerol are hyper-represented; and in group stimulated with addition of LHr and matured in culture medium only with FSH (LF group) the polyketide (flavonoid) and sphingolipids are hyper-represented. Conclusion: The protocol used for hormonal stimulation and MIV modify the lipid profile of the oocyte, but does not alter the oocyte maturation rate and the DNA integrity of cumulus cells.
- ItemSomente MetadadadosEfeito do hormônio luteinizante (LH) adicionado ao estímulo ovariano no perfil lipídico do líquido folicular de mulheres em tratamento para infertilidade(Universidade Federal de São Paulo (UNIFESP), 2013-07-31) Costa, Livia do Vale Teixeira da [UNIFESP]; Fraietta, Renato Fraietta [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objectives: Using Lipidomics approach to identify the follicular fluid lipid profile of women submitted to different ovarian stimulation protocols for in vitro fertilization treatments. Method: We conducted the study using 28 self-paired samples of follicular fluid from women who presented only tubal factor as a cause of female infertility. The patients were divided into (i) FSH group, consisting of 14 patients who received FSH hormone during controlled ovarian stimulation, and (ii) FSH + LH group, composed of the same 14 patients who have returned to the sector of Assisted Human Reproduction to receive a new stimulus, which corresponded to the addition of LH to the previous protocol. Lipidomics analysis was performed by MSE mass spectrometry. Possible lipids were identified by the software SimLipid 3.4. Results: Clinical data analysis showed that there are no statistically significant differences between groups. Lipids were identified in relation to their groups, so that in the FSH group we found wax monoesters, anthocyanidins and CoA fatty acyls, while in FSH + LH group we found sphingomyelin. Conclusion: Differences were found between the two groups concerning lipid profiles analyzed in follicular fluid. These findings contributed to deepen the understanding of the molecular mechanisms involved in the administration of different hormonal stimuli for patients undergoing treatments for infertility, and so to the establishment of the best protocol for each women considering the identification of potential biomarkers.
- ItemAcesso aberto (Open Access)Effects of psychostimulants and antipsychotics on serum lipids in an animal model for schizophrenia(MDPI AG, 2021-02-26) Correia, Banny Silva Barbosa; Nani, João Victor [UNIFESP]; Ricardo, Raniery Waladares; Stanisic, Danijela; Costa, Tássia Brena Barroso Carneiro; Hayashi, Mirian Akemi Furuie [UNIFESP]; Tasic, Ljubica; http://lattes.cnpq.br/5559309395232147Schizophrenia (SCZ) treatment is essentially limited to the use of typical or atypical antipsychotic drugs, which suppress the main symptoms of this mental disorder. Metabolic syndrome is often reported in patients with SCZ under long-term drug treatment, but little is known about the alteration of lipid metabolism induced by antipsychotic use. In this study, we evaluated the blood serum lipids of a validated animal model for SCZ (Spontaneously Hypertensive Rat, SHR), and a normal control rat strain (Normotensive Wistar Rat, NWR), after long-term treatment (30 days) with typical haloperidol (HAL) or atypical clozapine (CLZ) antipsychotics. Moreover, psychostimulants, amphetamine (AMPH) or lisdexamfetamine (LSDX), were administered to NWR animals aiming to mimic the human first episode of psychosis, and the effects on serum lipids were also evaluated. Discrepancies in lipids between SHR and NWR animals, which included increased total lipids and decreased phospholipids in SHR compared with NWR, were similar to the differences previously reported for SCZ patients relative to healthy controls. Administration of psychostimulants in NWR decreased omega-3, which was also decreased in the first episode of psychosis of SCZ. Moreover, choline glycerophospholipids allowed us to distinguish the effects of CLZ in SHR. Thus, changes in the lipid metabolism in SHR seem to be reversed by the long-term treatment with the atypical antipsychotic CLZ, which was under the same condition described to reverse the SCZ-like endophenotypes of this validated animal model for SCZ. These data open new insights for understanding the potential influence of the treatment with typical or atypical antipsychotics on circulating lipids. This may represent an outcome effect from metabolic pathways that regulate lipids synthesis and breakdown, which may be reflecting a cell lipids dysfunction in SCZ.
- ItemAcesso aberto (Open Access)Estudo de assinaturas metabólicas em leiomiomas uterinos(Universidade Federal de São Paulo, 2022-07-12) Barison, Gustavo Anderman Silva [UNIFESP]; Gomes, Mariano Tamura Vieira [UNIFESP]; Bonduki, Claudio [UNIFESP]; Castro, Rodrigo de Aquino [UNIFESP]; http://lattes.cnpq.br/6590913930590292; http://lattes.cnpq.br/7384818983129643; http://lattes.cnpq.br/6319329004052486; http://lattes.cnpq.br/7168607477194335Objective: The present study evaluated circulating metabolites in the plasma of patients with and without leiomyomas, to define a metabolomic profile of these patients and compare them according to leiomyomas' presence and uterine size. Methods: Cross-sectional observational study, including women divided into three groups: 37 with leiomyomas and uterus over 500 cm3, 17 with leiomyomas and uterus up to 150 cm3, and 21 leiomyoma-free women. Patients underwent peripheral blood collection that was further evaluated using untargeted metabolic assessment by gas chromatography coupled to mass spectrometer. Results: There was no statistical difference between patients’ anthropometric and demographic features and general laboratory tests. Groups were statistically different for uterus volume (p<0.0001). Forty six metabolites were identified in all samples (35% were amino acids and derivatives, 22% were fatty acids and 18% were carbohydrates). Statistically significant metabolic distinction (p<0.05 and FDR<0.05) was observed for 14 metabolites. Amino acids, except for L-glutamine, were significantly reduced in plasma levels of patients with large leiomyomas. Fatty acids and carbohydrates were progressively reduced in patients with leiomyomas and even more reduced in plasma levels of patients with large leiomyomas, except for an increase in alpha- linolenic acid. Conclusion: There are differences in plasma metabolites levels of amino acids, fatty acids, and carbohydrates among patients with leiomyomas and large leiomyomas compared to leiomyoma-free patients. These metabolites are especially reduced in patients presenting large leiomyomas. This metabolic panel is an initial step for the definition of possible biomarkers and metabolic signatures of the uterine fibroid. Keywords: Leiomyoma, Metabolomics, Mass spectrometry, Lipidomics, Gas chromatography, Omic.
- ItemAcesso aberto (Open Access)Estudo do efeito de duas terapias hipolipemiantes em indivíduos com infarto agudo do miocárdio: uma abordagem lipidômica(Universidade Federal de São Paulo, 2024-06-05) Bastos, Rafaela Tudela [UNIFESP]; Klassen, Aline [UNIFESP]; http://lattes.cnpq.br/5905235296066398; http://lattes.cnpq.br/0913760611178974De acordo com a Organização Mundial da Saúde (OMS), as doenças cardiovasculares (DCV) permaneceram como a principal causa de morte em todo o mundo nos últimos 20 anos. Entre 2017 a 2021, a Sociedade Brasileira de Cardiologia estima que que mais de 7 milhões de pessoas morreram por esse motivo no país, sendo responsável por 30% dos óbitos no Brasil. As DCVS podem causar infarto agudo do miocárdio (IAM), o qual ocorre devido ao acúmulo de placas de gordura no interior das artérias coronárias. Dentre os fatores de risco às DCV, tem-se a elevada concentração plasmática da lipoproteína de baixa densidade (LDL-C) e a baixa concentração plasmática da lipoproteína de alta densidade (HDL-C) que podem promover o infarto agudo do miocárdio (IAM). A dislipidemia que é causada por alterações metabólicas que ocorrem em resposta a distúrbios nas etapas do metabolismo lipídico, é um dos principais determinantes da DCV. Logo, o perfil lipídico sérico sofrerá alterações que acabará aumentando a concentração do colesterol total (CT), triglicerídeos (TG), LDL e baixando a concentração de HDL, o que demonstra maior risco de aterosclerose, consequentemente IAM. Neste sentido, redutores de colesterol, como as estatinas, que reduzem a morbidade e a mortalidade cardiovascular, decorrente da doença aterosclerótica, tem sido empregada. Além disso, alguns estudos demonstraram que o uso contínuo de estatinas causa mudanças no perfil lipídico do plasma humano. Neste sentido, a lipidômica tem ganhado espaço dentro da área cardiovascular, na tentativa de um entendimento das variações das vias metabólicas as quais os lipídios participam. Sendo assim, a lipidômica e quimiometria apresentam-se como uma alternativa em potencial para o acompanhamento da evolução da doença, pré e pós uso contínuo de estatinas. Dentro deste cenário, o presente trabalho teve como objetivo avaliar, por meio de ferramentas quimiométricas, o efeito da associação de ezetimiba à sinvastatina, em comparação com a estatina isolada rosuvastatina, comumente empregada nos ambulatórios de rotina, em plasma de pacientes infartados, por meio da composição lipídica previamente determinada. Os resultados obtidos por meio das técnicas estatísticas utilizadas neste estudo apontam que a Sinvastatina e a ezetimiba apresentam potencial sinérgico na alteração dos níveis lipídicos. Por outro lado, as alterações percentuais foram maiores com o tratamento com rosuvastatina em comparação com o tratamento com sinvastatina + ezetimiba, para CE, FFA e CER, com aumento nos níveis de LPC.
- ItemSomente MetadadadosLipid fingerprinting in women with early-onset preeclampsia: A first look(Elsevier B.V., 2012-07-01) De Oliveira, Leandro [UNIFESP]; Camara, Niels Olsen S.; Bonetti, Tatiana [UNIFESP]; Lo Turco, Edson G. [UNIFESP]; Bertolla, Ricardo Pimenta [UNIFESP]; Moron, Antonio F. [UNIFESP]; Sass, Nelson [UNIFESP]; Cotrim Guerreiro Da Silva, Ismael Dale [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP); Hosp Maternidade Vila Nova CachoeirinhaObjectives: the aim of this preliminary study was to characterize the plasma lipid profiling of women with preeclampsia.Design and methods: Plasma samples of 8 pregnant women with early-onset preeclampsia and 8 normal pregnant women were evaluated. Lipids were extracted from plasma using the Bligh-Dyer protocol. the extracts were subjected to MALDI-MS. Data matrix was exported for partial least squares discriminant analysis (PLS-DA) and a parameter VIP was employed to reflect the variable importance in the discriminant analysis. the major discriminant variables were selected and underwent to Mann-Whitney U test.Results: A total of 1290 ions were initially identified and twelve m/z signals were highlighted as the most important lipids for the discrimination of patients with preeclampsia. the identification of these differential lipids was carried out through Lipid Database Search.Conclusions: the main classes identified were glycerophosphocholines [GP01], glycerophosphoserines [GP03], glycerophosphoglycerols [GP04], glycosyldiradylglycerols [GL05] and glycerophosphates [GP10]. (C) 2012 the Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
- ItemEmbargoMetabolômica e lipidômica no plasma de mulheres soldadoras: exposição ocupacional a elementos potencialmente tóxicos e os impactos na saúde pública(Universidade Federal de São Paulo, 2024-09-27) Araújo, Alda Neis Miranda de [UNIFESP]; Assunção, Nilson Antônio de [UNIFESP]; Olympio, Kelly Polido Kaneshiro [USP]; Ferreira, Vinícius Guimarães [USP]; http://lattes.cnpq.br/5138426222420186; http://lattes.cnpq.br/9376176929300767; http://lattes.cnpq.br/4183619506352119; http://lattes.cnpq.br/8116599826565900Introdução: A exposição ocupacional a elementos potencialmente tóxicos (EPTs) representa um risco significativo à saúde e pode afetar o metabolismo humano. Objetivos: Avaliar o impacto da exposição ocupacional à diferentes EPTs no perfil metabólico e lipídico de mulheres engajadas na atividade de soldagem de joias domiciliar e informal na cidade de Limeira, Brasil. Métodos: Amostras de sangue de 64 participantes foram divididas em grupo Exposto (n = 36) e grupo Controle (n = 28). Os níveis sanguíneos de EPTs foram determinados por ICP-MS, enquanto os metabólitos e lipídios presentes nas amostras de sangue foram analisados por cromatografia liquida acoplado à espectrometria de massa (LC-MS). Os dados obtidos foram analisados empregando o software Progenesis™ QI v2.4, e o Metaboanalyst 6.0. Subsequentemente, foram realizadas análises univariadas, incluindo o teste t de student e a correlação de Spearman, além de análises multivariadas como a Análise de Componentes Principais (PCA). Resultados: Diferenças significativas foram encontradas nas concentrações de Pb (p < 0,001), As (p < 0,055) e Cd (p < 0,003) entre os grupos. Dos features considerados significativos para o estudo, foram identificados 190 metabólitos e 34 lipídeos. A correlação de spearman apontou correlações moderadas (r > 0,3) e significativas (p value < 0,05) para 54 metabólitos e 11 lipídios com os níveis de EPTs, incluindo, L Fenilalanina com Pb, Cd e As; L-Acetilcarnitina com As. Além disso, a análise de enriquecimento de vias revelou enriquecimentos em vias como, o metabolismo de purina, metabolismo de fenilalanina e tirosina, e oxidação de ácidos graxos. Paralelamente, a análise lipidômica destacou vias como distúrbios do transportador ABC, metabolismo de glicerolipídeos e homeostase da glicose, ambas indicando impactos diretos na fisiologia humana. Conclusões: Este estudo revelou metabólitos e lipídios correlacionados com baixas concentrações de Pb, As, Cd, Sb, Sn, Mn e Zn no sangue. A análise de enriquecimento de vias elucidou ainda mais o potencial impacto da exposição a EPTs em processos biológicos, com possíveis implicações para a saúde. Essas descobertas enfatizam a precariedade do trabalho informal, uma realidade de muitos países em desenvolvimento ao redor mundo.
- ItemSomente MetadadadosMetabolomics and lipidomics analyses by H-1 nuclear magnetic resonance of schizophrenia patient serum reveal potential peripheral biomarkers for diagnosis(Elsevier Science Bv, 2017) Tasic, Ljubica; Pontes, Joao G. M.; Carvalho, Michelle S. [UNIFESP]; Cruz, Guilherme; Dal Mas, Carolines [UNIFESP]; Sethi, Sumit [UNIFESP]; Pedrini, Mariana [UNIFESP]; Rizzo, Lucas B. [UNIFESP]; Zeni-Graiff, Maiara [UNIFESP; Asevedo, Elson [UNIFESP]; Lacerda, Acioly L. T. [UNIFESP]; Bressan, Rodrigo A. [UNIFESP]; Poppi, Ronei Jesus; Brietzke, Elisa [UNIFESP]; Hayashi, Mirian A. F. [UNIFESP]Using H-1 NMR-based metabolomics in association to chemometrics analysis, we analyzed here the metabolic differences between schizophrenia patients (SCZ) compared to healthy controls (HCs). HCs and SCZ patients underwent clinical interview using the Structured Clinical Interview for DSM Disorders (SCID). SCZ patients were further assessed by Positive and Negative Syndrome Scale (PANSS), Calgary Depression Scale, Global Assessment of Functioning Scale (GAF), and Clinical Global Impressions Scale (CGI). Using the principal component analysis (PCA) and supervised partial least-squares discriminate analysis (PLS-DA) in obtained NMR data, a clear group separation between HCs and SCZ patients was achieved. Interestingly, all metabolite compounds identified as exclusively present in the SCZ group, except for the gamma-aminobutyric acid (GABA), were never previously associated with mental disorders. Although the initial perception of an absence of obvious biological link among the different key molecules exclusively observed in each group, and no identification of any specific pathway yet, the present work represents an important contribution for the identification of potential biomarkers to inform diagnosis, as it was possible to completely separate the affected SCZ patients from HCs, with no outliers or exceptions. In addition, the data presented here reinforced the role of the modulation of glycolysis pathway and the loss of GABA intemeuron/hyperglutamate hypothesis in SCZ. (C) 2016 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosPerfil de hormônios e lipídios circulantes em pacientes com lipodistrofia generalizada congênita em resposta à ingestão alimentar(Universidade Federal de São Paulo (UNIFESP), 2020-07-31) Araujo, Camilla Oliveira Duarte De [UNIFESP]; Ribeiro, Eliane Beraldi [UNIFESP]; Universidade Federal de São PauloPatients with congenital generalized lipodystrophy (CGL) are not able to store lipids in the adipose tissue, which causes hypoleptinemia, increased appetite, ectopic fat deposition and lipotoxicity. However, a direct comparison between CGL and eutrophic individuals is lacking, regarding both appetite parameters and acylated ghrelin levels, the hormone form that is active in acute food intake stimulation. Moreover, the consequences of the derangements of lipid metabolism on plasma lipids have not been fully examined. The objective of the present study was to address whether and in what extent the subjective appetite parameters and acylated ghrelin response to a meal are affected in CGL individuals, in comparison to eutrophic individuals. An obese group was included in the study, to allow the comparison between a leptin-resistant and a leptin-deficient condition on these aspects. Additionnaly, the plasma lipidomic profile of CGL patients, in comparison to eutrophic individuals, was evaluated at the fasted state and after the intake of a meal. Eutrophic controls (EUT, n=10), obese subjects (OB, n=10) and CGL (n=11) were fasted overnight and then received an ad libitum meal. Blood was collected and the visual analogue scale was applied before and 90 minutes after the meal. Additional blood samples were collected at 30 and 60 minutes for ghrelin determinations. In the CGL patients and eutrophic controls, non-target lipidomics was performed by liquid chromatography–mass spectrometry. The CGL patients showed low fasting levels of leptin and adiponectin, dyslipidemia, and insulin resistance. The caloric intake was similar among the 3 groups. However, both CGL and OB had shorter satiation times than EUT. The CGL patients also had lower satiety time and their sensation of hunger was less attenuated by the meal. Fasting acylated ghrelin levels were lower in CGL than in EUT. After the meal, the levels tended to decrease in EUT but not in CGL and OB individuals. Regarding the lipidomic analysis, clear differences were detected between the groups. Several molecular species of fatty acyls, glycerolipids, glycerophospholipids, and sphyngolipids were altered in the CGL group. All the detected molecular species of fatty acyls were upregulated. Increments of several species of diacylglycerols and of one triacylglycerols species were also observed in the CGL group. Among the glycerophospholipids, we observed alterations of some glycerophosphoethanolamines and glycerophosphoserines species, and of one species of cardiolipins. Among the sphingolipids, one sphingomyelin and one glycosphingolipid species showed downregulation in CGL. The data indicate that CGL patients present appetite disturbances in relation to eutrophic individuals. Their low fasting levels of acylated ghrelin and the absence of the physiological drop after refeeding suggest a role of these disturbances in hunger attenuation and satiety but not in acute satiation. The alterations in the lipid profile of CGL patients indicate that, besides the direct impact of the impairment of TAGs synthesis and lipid droplets formation, the metabolism of complex lipids is also affected in CGL patients. The findings highlight the importance of a deeper comprehension of the role of specific lipid categories in the metabolic derangements present in CGL.
- ItemSomente MetadadadosRecent advances in lipidomics: Analytical and clinical perspectives(Elsevier Science Inc, 2017) Sethi, Sumit [UNIFESP]; Brietzke, Elisa [UNIFESP]Lipidomics or lipid-profiling is a lipid-targeted metabolomics approach aiming at comprehensive analysis of lipids in biological systems. The extent of information in the genomic and proteomic fields is greater than that in the lipidomics field, because of the complex nature of lipids and the limitations of tools for analysis. Modern technological advances in mass spectrometry and chromatography have greatly improved the developments and applications of metabolic profiling of diverse lipids in complex biological samples. Lipidomics will not only provide insights into the specific functions of lipid species in health and disease, but will also identify potential biomarkers for establishing preventive or therapeutic programs for human diseases. In this review, emphasis is given to the current advances in lipidomics technologies and their applications in disease biomarker discovery, and its clinical application. The application oflipidomics in clinical studies may provide new insights into lipid profiling and pathophysiological mechanisms. We also discuss the lipidomics for the future perspectives and their potential problems. (C) 2017 Elsevier Inc. All rights reserved.