Navegando por Palavras-chave "Lamiaceae"

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    Inhibition of the gastric H+,K+-ATPase by plectrinone A, a diterpenoid isolated from Plectranthus barbatus Andrews
    (Elsevier B.V., 2007-04-20) Schultz, Carla; Bossolani, Myllene P.; Torres, Luce M. B.; Lima-Landman, Maria Teresa R.; Lapa, Antonio J.; Souccar, Caden; Universidade Federal de São Paulo (UNIFESP); Bot Garden São Paulo
    This work assessed the mechanism underlying the antisecretory gastric acid effect of Plectranthus barbatus Andrews (Lamiaceae) and active constituents. Popularly known as false-boldo, this plant is used in Brazilian folk medicine to treat gastrointestinal and hepatic ailments. the plant aqueous extract (AE) and isolated compounds were assayed in vivo in pylorus-ligated mice, and in vitro on acid secretion measured as [C-14] -arninopyrine ([C-14]-AP) accumulation in rabbit gastric glands and gastric H+,K+-ATPase preparations. Injected into the duodenal lumen, the AE of the plant leaves (0.5 and 1.0g/kg) decreased the volume (62 and 76%) and total acidity (23 and 50%) of gastric acid secretion in pylorus-ligated mice. Bioguided purification of the AE yielded an active fraction (IC50 = 24 kg/ml) that inhibited acid secretion in rabbit gastric glands with a potency 10 to 18 times greater than that of the originating extract, on both the basal and stimulated acid secretion by histamine (His) (1 mu M) or bethanechol (100 mu M). At the same concentrations the gastric H+,K+-ATPase activity was also inhibited. the active constituent was chemically identified as the abietanoid dienedione plectrinone A which reduced the H+,(+)-ATPase activity with IC50 = 171 mu M. the results indicate that inhibition of the gastric proton pump by this diterpenoid may account for the antisecretory acid effect and reputed antiulcer activity of Plectranthus barbatus. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
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    Ocimum gratissimum Essential Oil and Its Isolated Compounds (Eugenol and Myrcene) Reduce Neuropathic Pain in Mice
    (Georg Thieme Verlag Kg, 2016) Paula-Freire, Lyvia Izaura Gomes [UNIFESP]; Molska, Graziella Rigueira [UNIFESP]; Andersen, Monica Levy [UNIFESP]; Carlini, Elisaldo Araujo [UNIFESP]
    Ocimum gratissimum is used in popular medicine to treat painful diseases. The antihypernociceptive properties of O. gratissimum essential oil and two of its active components (eugenol and myrcene) were tested in a model of neuropathic pain induced by a chronic constriction injury of the sciatic nerve. In tests to determine chronic antinociception, adult male C57BL/6 J mice were treated orally with corn oil (control group), O. gratissimum essential oil at doses of 10, 20, or 40 mg/kg or eugenol or myrcene at doses of 1, 5, or 10 mg/kg for 14 days after surgery. Pregabalin (20mg/ kg) was used as a standard in this study. The treatment with 20 and 40mg/kg of O. gratissimum essential oil and at doses of 5 and 10 mg/kg of the active components were able to promote antihypernociception in both mechanical (von Frey) and thermal (hot plate) tests. The treatment with the essential oil of the plant or eugenol was effective in reducing the levels of interleukin-1 beta in the sciatic nerve. Our findings demonstrate that O. gratissimum essential oil and its isolated active components possess antihypernociceptive activity in neuropathic pain models.
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    Ocimum gratissimum Essential Oil and Its Isolated Compounds (Eugenol and Myrcene) Reduce Neuropathic Pain in Mice
    (Georg Thieme Verlag Kg, 2016) Paula-Freire, Lyvia Izaura Gomes [UNIFESP]; Molska, Graziella Rigueira [UNIFESP]; Andersen, Monica Levy [UNIFESP]; Carlini, Elisaldo Araujo [UNIFESP]
    Ocimum gratissimum is used in popular medicine to treat painful diseases. The antihypernociceptive properties of O. gratissimum essential oil and two of its active components (eugenol and myrcene) were tested in a model of neuropathic pain induced by a chronic constriction injury of the sciatic nerve. In tests to determine chronic antinociception, adult male C57BL/6 J mice were treated orally with corn oil (control group), O. gratissimum essential oil at doses of 10, 20, or 40 mg/kg or eugenol or myrcene at doses of 1, 5, or 10 mg/kg for 14 days after surgery. Pregabalin (20mg/ kg) was used as a standard in this study. The treatment with 20 and 40mg/kg of O. gratissimum essential oil and at doses of 5 and 10 mg/kg of the active components were able to promote antihypernociception in both mechanical (von Frey) and thermal (hot plate) tests. The treatment with the essential oil of the plant or eugenol was effective in reducing the levels of interleukin-1 beta in the sciatic nerve. Our findings demonstrate that O. gratissimum essential oil and its isolated active components possess antihypernociceptive activity in neuropathic pain models.