Repositório Institucional - Unifesp :: Navegando por Palavras-chave "Keratitis"

Navegando por Palavras-chave "Keratitis"

Agora exibindo 1 - 20 de 29

Acesso aberto (Open Access)
Análise laboratorial das ceratites infecciosas secundárias à cirurgia refrativa
(Conselho Brasileiro de Oftalmologia, 2005-06-01) Leal, Fernando [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Freitas, Denise de [UNIFESP]; Campos, Mauro Silveira de Queiroz [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
PURPOSE: To describe the laboratory findings in patients with infectious keratitis, who underwent refractive surgery, correlating the surgical procedure and the time of infection manifestation, and the results of culture and smears. METHODS: The previous samples were obtained from patients submitted to radial keratotomy (RK), photorefractive keratectomy (PRK) and laser in situ keratomileusis (LASIK) at the Ophthalmology Department of UNIFESP. The infections were classified as early, when they occurred up to 30 days after the surgery, and late when diagnosed after 30 days. RESULTS: In 93 samples, 39 (42%) came from patients submitted to radial keratotomy, 14 (36%) being early infections and 25 (64%) late; 38 (41%) of LASIK, 21 (55%) being early and 17 (45%) late; 16 (17%) of photorefractive keratectomy, 10 (62.5%) being early and 6 (37.5%) late. Eighty-six samples were submitted to culture and smears for bacteria, 43 cultures (50%) and 43 smears (50%) were positive. Seventy-two samples were submitted to culture and smears for fungi, 2 cultures (3%) and 4 smears (6%) were positive. CONCLUSION: The agreement between culture and smear results was 80.2%; regarding the type of surgery there was no statistically significant relationship between time of onset of infection and surgery.
Acesso aberto (Open Access)
Antifungal susceptibility testing of yeast isolated from corneal infections
(Conselho Brasileiro de Oftalmologia, 2003-10-01) Mascaro, Vera Lucia Degaspare Monte [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Gompertz, Olga Fischman [UNIFESP]; Yu, Maria Cecília Zorat [UNIFESP]; Matta, Daniel Archimedes da [UNIFESP]; Colombo, Arnaldo Lopes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Hospital do Servidor Público Estadual Cornea Sector
PURPOSE: To report the antifungal susceptibility profile of yeast isolates obtained from cases of keratitis. METHODS: Susceptibility testing of 15 yeast strains isolated from corneal infections to amphotericin B, fluconazole, itraconazole and ketoconazole was performed using the NCCLS broth microdilution assay. RESULTS: Most episodes of eye infections were caused by Candida albicans. The antifungal drugs tested showed the following minimal inhibitory concentration values against yeast isolates: 0.125-0.5 µg/ml for amphotericin B; 0.125->64.0 µg/ml for fluconazole; 0.015-1.0 µg/ml for itraconazole and 0.015-0.125 µg/ml for ketoconazole. Despite the fact that all Candida isolates were judged to be susceptible to azoles, one isolate showed a minimal inhibitory concentration value significantly higher than a 90% minimal inhibitory concentration of all tested isolates. Rhodotorula rubra was resistant to fluconazole and itraconazole. CONCLUSIONS: Despite the fact that most yeast isolates from corneal infections are usually susceptible to amphotericin B and azoles, they exhibit a wide range of minimal inhibitory concentration values for antifungal drugs. The identification of strains at species level and their susceptibility pattern to antifungal drugs should be considered before determining the concentration to be used in topical antifungal formulations in order to optimize therapeutic response in eye infections.
Somente Metadadados
Antimicrobial susceptibility of photodynamic therapy (UVA/riboflavin) against Staphylococcus aureus
(Consel Brasil Oftalmologia, 2012-11-01) Kashiwabuchi, Renata Tiemi [UNIFESP]; Khan, Yasin; Carvalho, Fabio Ramos de Souza [UNIFESP]; Hirai, Flávio Eduardo [UNIFESP]; Campos, Mauro Silveira de Queiroz [UNIFESP]; McDonnell, Peter John; Universidade Federal de São Paulo (UNIFESP); Johns Hopkins Univ
Purpose: To assess S. aureus in vitro viability after the exposure to ultraviolet light A (UVA) and riboflavin (B2).Methods: Samples of S. aureus in 96 well plates (in triplicate) were exposed to riboflavin (B2) and ultraviolet light A (365 nm wavelength) at a power density of 3 mW/cm(2), 8 mm spot diameter, for 30 minutes. Control groups were prepared as well in triplicate: blank control, ultraviolet light A only, riboflavin only and dead bacteria Control. The bacterial viability was measured using fluorescent microscopy. In order to investigate the occurrence of viable but non-culturable microorganisms after treatment, the cell viability was also investigated by plate culture procedure onto a broth medium. Statistical analysis was performed using the triplicate values from each experimental condition.Results: No difference was observed among the treatment group and the control samples (p=1).Conclusion: The combination of riboflavin 0.1% and ultraviolet light A at 365 nm did not exhibit antimicrobial activity against oxacillin susceptible S. aureus.
Acesso aberto (Open Access)
Características laboratoriais das ceratites e conjuntivites causadas por Streptococcus sp
(Conselho Brasileiro de Oftalmologia, 2004-10-01) Solari, Helena Parente [UNIFESP]; Sousa, Luciene Barbosa de [UNIFESP]; Freitas, Denise de [UNIFESP]; Yu, Maria Cecília Zorat [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
PURPOSE: To evaluate laboratorial findings of Streptococcus keratitis and conjunctivitis, analyzing the different species and the results of bacterial susceptibility to an antibiotics. METHODS: Retrospective study of the records from the External Disease Laboratory of the Ophthalmology Department of the Federal University of São Paulo, with conjunctival or corneal positive bacterial culture for Streptococcus sp, between January 1995 and December 2001. The collected data were age, Streptococcus species and the bacterial susceptibility to the following antibiotics: cephalotin, amikacin, gentamicin, tobramicin, ciprofloxacin, lomefloxacin, ofloxacin, norfloxacin and vancomicin. RESULTS: The most frequent species were Streptococcus pneumoniae and Streptococcus viridans. Regarding bacterial susceptibility to antibiotics we found a higher susceptibility to the following antibiotics: cephalotin, quinolones and vancomicin. CONCLUSIONS: Considering the commercially available topic antibiotics, the quinolones presented better results when compared to the aminoglycosides.
Acesso aberto (Open Access)
Ceratite infecciosa em crianças: estudo microbiológico e epidemiológico em um hospital universitário de Assunção - Paraguai
(Conselho Brasileiro de Oftalmologia, 2005-12-01) Maidana, Eduardo; González, Richard; Melo Júnior, Luiz Alberto Soares De [UNIFESP]; Souza, Luciene Barbosa De [UNIFESP]; Universidade Nacional de Assunção Hospital de Clínicas Departamento de Oftalmologia; Universidade Federal de São Paulo (UNIFESP)
PURPOSE: To describe the microbiologic aspects of infectious keratitis in children (0-18 years old) examined at the Department of Ophthalmology of the National University of Asunción - Paraguay, during a 14-year period (1988-2002). METHODS: The authors retrospectively studied 146 children, under 18 years old, with infectious keratitis at the Department of Ophthalmology of the National University of Asunción - Paraguay, from 1988 to 2002. RESULTS: The cultures were positive in 113 patients (77%) with 70% of bacterial and 30% of fungal etiology. Coagulase-negative Staphylococcus (23.1%), Staphylococcus aureus (19.2%), Pseudomonas aeruginosa (12.8%) and Streptococcus pneumoniae (12.8%) were the most common microorganisms isolated. Acremonium ssp (25%), Fusarium ssp (14%) and Aspergillus fumigatus (14%) were the fungi isolated. CONCLUSIONS: Ulcerative keratitis in children examined at the Department of Ophthalmology of the National University of Asunción - Paraguay is of bacterial origin in most of the cases.
Acesso aberto (Open Access)
Ceratite por Staphylococcus aureus após ceratomileuse in situ por laser (LASIK): relato de caso
(Conselho Brasileiro de Oftalmologia, 2000-04-01) Chaves, Alessandra Pinheiro [UNIFESP]; Schor, Paulo [UNIFESP]; Sartori, Marta [UNIFESP]; Campos, Mauro Silveira de Queiroz [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Purpose: To report a case of S. aureus keratitis after laser in situ keratomileusis, the management and the outcome. Case report: 26-year-old male underwent toric LASIK OS, evoluing with S. aureus keratitis. After a clinical hypothesis of infection, we performed corneal scrape for lab analysis and proper treatment was given. After successful treatment, the patient showed an improvement in corneal transparency and recovered his visual acuity. Discussion: Infections following laser in situ keratomileusis may not produce visual loss if proper treatment is given.
Acesso aberto (Open Access)
Ceratoconjuntivite cicatricial bilateral associada a líquen plano: relato de caso
(Conselho Brasileiro de Oftalmologia, 2008-12-01) Diniz, Camila Melo Gadelha Pereira [UNIFESP]; Vieira, Luiz Antonio [UNIFESP]; Rigueiro, Moacyr Pezati [UNIFESP]; Vasconcelos, Mônica [UNIFESP]; Freitas, Denise de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
To describe a case of bilateral cicatrizing keratoconjunctivitis in a patient with lichen planus and review the literature. Conjunctiva cicatrization with symblepharon formation, dry eye, corneal infiltration and neovascularization and thinning were the most observed prominent signs. Diagnosis was based on clinical findings and biopsy, after exclusion of typical causes of cicatricial keratoconjuntivitis.
Acesso aberto (Open Access)
Clinical treatment of corneal infection due to Fonsecaea pedrosoi: case report
(Conselho Brasileiro de Oftalmologia, 2005-04-01) Hofling-Lima, Ana Luisa [UNIFESP]; Guarro, Josep; Freitas, Denise de [UNIFESP]; Godoy, Patricio [UNIFESP]; Gené, Josepa [UNIFESP]; Souza, Luciene Barbosa de [UNIFESP]; Zaror, Luis; Romano, Andre C. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universitat Rovira I Virgili Facultat de Medicina I Ciencies de la Salut; University Austral de Chile Microbiology Unitat Clinic
To report an unusual case of fungus keratitis due to Fonsecaea pedrosoi that developed after corneal trauma. Case report: A 18-year-old male presented with a corneal ulcer in the right eye, 28 days after a trauma with glass fragments. Corneal scrapings were collected for smears and culture. Dematiaceous hyphae were seen on wet mounts of the scrapings and dark pigmented colonies grew repetitively on the culture media; all colonies were identical, and were subsequently identified as Fonsecaea pedrosoi. Treatment was initiated with topical natamycin at one hour intervals, 200 mg oral ketoconazole per day and later changed to a combination of 200 mg ketoconazole and amphotericin B. In humid tropical regions Fonsecaea pedrosoi is one of the primary causes of human chronic cutaneous mycosis, chromoblastomycosis. Combination of systemic and topical antifungal medications may provide the best option for cure in corneal chromoblastomycosis.
Acesso aberto (Open Access)
Contact lens-associated microbial keratitis
(Conselho Brasileiro de Oftalmologia, 2008-12-01) Moriyama, Aline Silveira [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
PURPOSE: Contact lens-associated microbial keratitis is a severe condition with sight-threatening potential and increasing incidence. Information regarding the etiological agents is essential in guiding management and may vary geographically. The aim of this study was to analyze the microbiological results of corneal scrapings collected from patients presenting with contact lens-associated microbial keratitis. METHODS: Retrospective analysis of the records of all patients who were clinically diagnosed with contact lens-associated microbial keratitis and had corneal scrapings sent to the Laboratory of Ocular Microbiology, UNIFESP/EPM during a 5-year period from January 2002 to December 2007. RESULTS: The etiological agent was identified in 239 patients. Bacterial isolates accounted for 166 (69.46%) cases, Acanthamoeba for 95 (39.75%) cases and fungi for 4 (1.67%) cases. Among the bacterial infections, coagulase-negative Staphylococcus was demonstrated in 74 cases, while Pseudomonas spp was found in 32 patients. All coagulase negative Staphylococcus and Pseudomonas were susceptible to ciprofloxacin and ofloxacin. Resistance to gentamicin was documented in a single case of Pseudomonas. Fourth-generation flouoroquinolone resistance was not observed among Pseudomonas cases. CONCLUSION: Coagulase-negative Staphylococcus was the most frequent isolate, and such data must be considered when determining empiric treatment. Second-generation fluoroquinolones ciprofloxacin and ofloxacin and fourth-generation fluoroquinolones moxifloxacin and gatifloxacin showed a good antibacterial profile and therefore could be good options for initial management.
Acesso aberto (Open Access)
Deep stromal mycobacterial keratitis: viable bacteria after six months of treatment: case report and literature review
(Conselho Brasileiro de Oftalmologia, 2005-08-01) Gusmão, Filipe Brandão Accioly de [UNIFESP]; Alvarenga, Lênio [UNIFESP]; Barbosa, Luciene [UNIFESP]; Sampaio, Jorge; Leao, Sylvia Cardoso [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Freitas, Denise de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Laboratório Fleury
To report the presence of viable mycobacteria in a patient with keratitis treated for 6 months. Species identification was performed using the PRA method (polymerase chain reaction followed by restriction endonuclease analysis). Clonality was evaluated with RAPD (randomly amplified polymorphic DNA) and ERIC-PCR (enterobacterial repetitive intergenic consensus - polymerase chain reaction) methods. The patient reported trauma due to a metallic foreign body 3 weeks prior to presentation. Initial corneal scraping cultures revealed Mycobacterium abscessus. After 6 months of topical and systemic treatment the patient presented with no active inflammation and was considered clinically cured. An optic penetrating keratoplasty was performed. Culture of the excised cornea revealed Mycobacterium abscessus. Both isolates had the same clonal origin. The most interesting finding of this case report was the positive culture of the excised cornea after 6 months of intensive specific topical therapy. To our knowledge, this is the first report in the literature showing this possibility in the treatment of Mycobacterial keratitis. Thus, Mycobacterium abscessus may present viable bacteria after long-term treatment and should be followed carefully for a long period of time after tapering the medication.
Acesso aberto (Open Access)
Distribuição das ceratites infecciosas em hospital terciário no Brasil
(Conselho Brasileiro de Oftalmologia, 2013-12-01) Marujo, Fábio Iglesias; Hirai, Flávio Eduardo [UNIFESP]; Yu, Maria Cecília Zorat [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Freitas, Denise de [UNIFESP]; Sato, Elcio Hideo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
PURPOSE: To investigate the seasonality of infectious keratitis in a tertiary hospital in Brazil. METHODS: Charts from the Ocular Microbiology Laboratory of the Department of Ophthalmology of the Federal University of São Paulo were reviewed from 2005 to 2009. Infectious keratitis were defined as those with positive culture. Data were distributed monthly and linear regressions with seasonal dummy models were used to test for seasonal trends. RESULTS: Total of 1,468 cases of keratitis was diagnosed during the study period. Bacterial keratitis were reponsible for 80.3% of all cases, followed by fungal (7%), and Acanthamoeba (6%); 6.7% were mixed cases. Statistical tests showed that there were no seasonal trends for bacteria (p=0.064), fungi (p=0.379), Acanthamoeba (p=0.062) or mixed infections (p=0.441). Conclusions: No seasonal trends for infectious keratitis were observed in our sample.
Acesso aberto (Open Access)
Effect of topical 0.5% povidone-iodine compared to 5% natamycin in fungal keratitis caused by Fusarium solani in a rabbit model: a pilot study
(Conselho Brasileiro de Oftalmologia, 2008-12-01) Oliveira, Lauro Augusto De [UNIFESP]; Takata, Thales Takeo [UNIFESP]; Shiguematsu, Alvio Isao [UNIFESP]; Melo Júnior, Luiz Alberto Soares [UNIFESP]; Gompertz, Olga Fischman [UNIFESP]; Sousa, Luciene Barbosa De [UNIFESP]; Mannis, Mark J.; Universidade Federal de São Paulo (UNIFESP); University of California
PURPOSE: To evaluate the efficacy of topical administration of 0.5% povidone-iodine in experimental Fusarium solani keratitis in rabbits. METHODS: Fungal keratitis caused by Fusarium solani was induced in the right eye of 24 New Zealand rabbits. The rabbits were randomly divided into 3 different treatment groups: Group I (povidone-iodine) - treated with topical 0.5% povidone-iodine; Group II (natamycin) - treated with topical 5% natamycin; and Group III (control) - treated with topical saline solution. In all groups the rabbits were treated for three days and then sacrificed. The corneas were excised, macerated and immersed in 10 mL BHI. Culture samples were plated daily on Sabouraud's agar for 7 days, and the number of colony-forming units (CFU) was counted. The rabbits were clinically evaluated during the treatment period. RESULTS: The povidone-iodine and natamycin groups demonstrated better efficacy than the control group based on the number of rabbits with no colonies growing. However, there were no statistically significant differences between the three groups when the number of CFU was analyzed (p>0.05). CONCLUSIONS: Our study demonstrates important methodological considerations in the use of in vivo animal models for the testing of antifungal agents. Using this sample size and methodology of counting CFU, topical 0.5% povidone-iodine demonstrated no benefit in the treatment of experimental Fusarium solani when compared with topical 5% natamycin.
Somente Metadadados
Estudo das alterações corneanas provocadas pela inoculação da aflatoxina B1 em coelhos
(Universidade Federal de São Paulo (UNIFESP), 1993) Vieira, Luiz Antonio [UNIFESP]; Gompertz, Olga Fischman [UNIFESP]
Somente Metadadados
Estudo experimental da eficácia da sulfadiazina de prata a 1
(Universidade Federal de São Paulo (UNIFESP), 1993) Sousa, Luciene Barbosa de [UNIFESP]; Scarpi, Marinho [UNIFESP]
Acesso aberto (Open Access)
Estudo laboratorial das micoses oculares e fatores associados às ceratites
(Conselho Brasileiro de Oftalmologia, 2005-02-01) Hofling-Lima, Ana Luisa [UNIFESP]; Forseto, Adriana dos Santos [UNIFESP]; Duprat, João Paulo [UNIFESP]; Andrade, Alfredo [UNIFESP]; Souza, Luciene Barbosa de [UNIFESP]; Godoy, Patrício [UNIFESP]; Freitas, Denise de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
PURPOSE: To analyze the frequency and the etiology of mycotic infectious eye diseases diagnosed by culture at the Ocular Diseases Laboratory of the Federal University of São Paulo (LOFT-UNIFESP), São Paulo, SP, Brazil, and the factors associated with fungal keratitis. METHODS: We retrospectively analyzed the 14,391 laboratory requests regarding infectious eye diseases between 1975 and May 2003. For the fungal culture proven examinations we observed the morphologic type of the isolated fungus, the gender and age of the patient, the site of ocular involvement, the year of identification, and the factors associated with keratitis cases, according to the identified etiologic agent. Results were considered significant if p<0.05. RESULTS: We verified 296 (2.05%) ocular mycosis cases with 265 keratitis (89.5%), 27 intraocular infections (9.1%), and 4 infections at other sites (1.3%). Filamentous fungi were isolated in 233 cases (78.7%) 74.7% being male (n=174) and 25.3% female (n=59). Yeasts were isolated in 63 cases (21.3%) 49.2% being male (n=31) and 50.8% female (n=32). Ages varied between 2 and 99 years in the filamentous fungi group and between 14 and 88 years in the yeast group. The most frequently found etiologic agents of the filamentous fungi isolations were Fusarium (n=137; 58.8%), Aspergillus (n=28; 12.0%), and Penicillium (n=12; 5.2%); and in yeasts, Candida (n=59; 93.7%). Regarding keratitis, 95.1% had at least one associated factor. Ocular trauma was significantly associated with filamentous fungi keratitis (p=0.0002); and previous surgeries (p=0.0002), ocular (p=0.0002) and systemic diseases (p=0.0002) with yeast keratitis. The use of topic antibiotics showed association with filamentous fungi keratitis, at a lower statistical significance (p=0.0224). CONCLUSIONS: Laboratory analysis showed predominance of filamentous fungi over the yeasts, and keratitis was the main observed ocular infection. Fungal keratitis involved at least one associated factor in most cases.
Acesso aberto (Open Access)
An experimental model of mycobacterial infection under corneal flaps
(Associação Brasileira de Divulgação Científica, 2004-07-01) Adan, Consuelo Bueno Diniz [UNIFESP]; Sato, Elcio Hideo [UNIFESP]; Sousa, Luciene Barbosa de [UNIFESP]; Oliveira, Rosangela Siqueira de [UNIFESP]; Leao, Sylvia Cardoso [UNIFESP]; Freitas, Denise de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
In order to develop a new experimental animal model of infection with Mycobacterium chelonae in keratomileusis, we conducted a double-blind prospective study on 24 adult male New Zealand rabbits. One eye of each rabbit was submitted to automatic lamellar keratotomy with the automatic corneal shaper under general anesthesia. Eyes were immunosuppressed by a single local injection of methyl prednisolone. Twelve animals were inoculated into the keratomileusis interface with 1 µl of 10(6) heat-inactivated bacteria (heat-inactivated inoculum controls) and 12 with 1 µl of 10(6) live bacteria. Trimethoprim drops (0.1%, w/v) were used as prophylaxis for the surgical procedure every 4 h (50 µl, qid). Animals were examined by 2 observers under a slit lamp on the 1st, 3rd, 5th, 7th, 11th, 16th, and 23rd postoperative days. Slit lamp photographs were taken to document clinical signs. Animals were sacrificed when corneal disease was detected and corneal samples were taken for microbiological analysis. Eleven of 12 experimental rabbits developed corneal disease, and M. chelonae could be isolated from nine rabbits. Eleven of the 12 controls receiving a heat-inactivated inoculum did not develop corneal disease. M. chelonae was not isolated from any of the control rabbits receiving a heat-inactivated inoculum, or from the healthy cornea of control rabbits. Corneal infection by M. chelonae was successfully induced in rabbits submitted to keratomileusis. To our knowledge, this is the first animal model of M. chelonae infection following corneal flaps for refractive surgery to be described in the literature and can be used for the analysis of therapeutic responses.
Acesso aberto (Open Access)
Expressão gênica de células da córnea submetidas à infecção por acanthamoeba Spp
(Universidade Federal de São Paulo (UNIFESP), 2018-06-28) Sant'Ana, Viviane Peracini [UNIFESP]; Carvalho, Fabio Ramos de Souza [UNIFESP]; http://lattes.cnpq.br/1910912718767159; http://lattes.cnpq.br/9578890417214515; Universidade Federal de São Paulo (UNIFESP)
Objective: The present research project proposes: 1) to analyze the proteolytic degradation of the main substrates present in the cornea (collagen, fibronectin, laminin and tubulin) with the Acanthamoeba exoproteome; 2) to analyze the cytotoxicity of the Acanthamoeba exoproteome of in HUVEC cells in 11 clinical isolates and which its main mechanism of induction of cell death; 3) to identify by benzamidinesepharose purification of the serine protease class; 4) to characterize the Acanthamoeba exoproteome at different pHs and temperatures; 5) to analyze the susceptibility of the Acanthamoeba exoproteome with diamidines and enzymatic inhibitors. Methods: Primary cultures of trophozoites were obtained by corneal scraping of patients with Acanthamoeba spp. Analysis of the Acanthamoeba exoproteome was performed by zymography (SDSPAGEgelatin), 0.01% gelatin polyacrylamide gel was prepared according to the subunits of its substrates: Fibronectin, Laminin and Tubulin. The HUVECs were cultured in a humid oven at 37 ° C to 5% CO2 in RPMI culture medium supplemented with 10% fetal bovine serum. The proteolysis assay of type I collagen was carried out by the hydrolysis of type I collagen, at a concentration of 0.4 mg / ml, previously dialyzed in specific buffer (50 mM TrisHCl, pH 7.5, 150 mM NaCl). In the analysis of the characterization of the Acanthamoeba exoproteome in different buffers of pHs and at different temperatures where each clinical isolate was removed from the gel and placed at different pHs (pHs 2.0 12.0). The diamidines used were 0.1% Brolene and 0.1% Désomédine, also 0.1 mM PMSF and 215 μM Trasylol enzyme inhibitors were used. Results: The proteolytic profile of Acanthamoeba exoproteome is associated with severity of infection observed in the patient. The degradation of type I collagen by the Acanthamoeba exoproteome the laboratory results corroborate with the clinical aspect observed in patients with moderate and severe amoebic keratitis. In the cytotoxicity assay, in vitro results from the Acanthamoeba exoproteome with HUVEC suggest the contact independence of trophozoite to induce cell death by apoptosis. We were able to demonstrate by in vitro protein assays, whose results prospect the mechanisms of gene expression in corneal cells, the functional aspects of different amoebic exoproteomes associated with healing and regeneration, whose processes are mainly mediated via cellular signaling in the host. Affinity chromatography demonstrated the prevalence of proteolytic enzymes of the class of serine proteases contained in the protozoan exoproteome. In the proteolysis assays of the Acanthamoeba exoproteome at different pH conditions and at different temperatures, it showed strong resistance to different pH and temperature conditions where they were evaluated in the period of 4 and 16 hours, where the optimum temperature was 35 ° C and 37 ° C in pH 7.0 appropriate conditions for the pathophysiology of amoebic keratitis. In the experiment with the diamidines, we noticed that unlike propamidine, hexamidine was not able to totally inhibit the enzymatic activity of the exoproteome. Conclusions: Exoproteome produced by Acanthamoeba spp trophozoites consists of a large diversity of enzymes, with emphasis on the enzymes belonging to the class of serine proteases. The enzymatic activity contained in the different amoebic exoproteomes suggests an important influence on the mechanisms of gene expression, with emphasis on the production of extracellular matrix compounds by corneal cells. The exoproteomes may present a pattern of physical and chemical resistance associated with enzymatic activity. The enzymatic constituents of different amoebic exoproteomes express proteins capable of degrading the main type I collagen and glycoproteins present in the cornea, besides inducing cell death by contact independent apoptosis. Laboratory isolation followed by amoebic exoproteome analysis demonstrates the importance of translational research associated with the present study, since the enzymatic constituents secreted by the protozoan can induce cell death and interferes in the synthesis of extracellular matrix compounds linked to the healing and regeneration processes tissue. Consequently, mechanisms associated with gene expression in epithelial cells and keratocytes can be inactivated, prospecting worsening in the clinical pattern of the patient.
Acesso aberto (Open Access)
Infectious keratitis in patients undergoing Boston Type 1 keratoprosthesis (Boston KPro) procedure: case series
(Conselho Brasileiro de Oftalmologia, 2011-04-01) Nascimento, Heloisa Moraes do [UNIFESP]; Oliveira, Lauro Augusto de [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Description of two cases of infectious keratitis in patients after Boston Type 1 keratoprosthesis (Boston KPro) implantation. The first case refers to a patient that had the device indicated due to limbal deficiency secondary to severe dry eye who presented a fungal infection by Aerobasidium pullulans that was successfully treated with amphotericin B eye drops. The second case reports a patient with Boston KPro implantation due to previous corneal transplant rejection showing bacterial keratitis in the fourth postoperative month. The etiologic agent was identified asStreptococcus sp and topical treatment with vancomycin was effective. The importance of postoperative surveillance in Boston KPro eyes is discussed.
Acesso aberto (Open Access)
Investigação experimental da utilidade da medicação no pós-operatório em cirurgia refrativa
(Conselho Brasileiro de Oftalmologia, 2005-04-01) Wahab, Sâmia Ali; Moreira, Hamilton [UNIFESP]; Buquera, Michele; Moreira, Luciane; Daros, Alessandra Castelano; Oliveira, Cínara Sakuma De; Instituto de Pesquisas Médicas; Universidade Federal de São Paulo (UNIFESP); Universidade Federal do Paraná; Faculdade Evangélica do Paraná; Hospital de Olhos do Paraná
PURPOSE: To harvest microorganisms from the microkeratome after LASIK. To induce experimental infectious keratitis and investigate the use of routine postoperative medication. METHODS: During eight consecutive days, we cultured the plate of the microkeratome after routine LASIK. Dilutions of the most frequent microorganism was prepared according to the MacFarland scale. Ten microliters were injected into both corneas of eight guinea pigs. The right cornea was used as control and the left cornea was treated with 0.3% ciprofloxacin associated with dexamethasone and ketorolac tromethamine every 4 hours for 24 hours (group 2). The animals were sacrificed and the corneas were divided into two halves, which were sent to analyzed by the microbiology and histopathology laboratory. RESULTS: In the control group, Staphylococcus aureus could be retrieved from all corneas, while in group 2, only 50% showed positive cultures (p=0.0128). Histopathology concerning polymorphonuclear cells showed an intense degree of inflammation in group 1 (p=0.0203) and regarding monomorphonuclear cells, it was also more intense in group 1 (p=0.0051). CONCLUSION: This study suggests that the use of wide-spectrum antibiotic associated with anti-inflammatory eye drops has a significant effect on the reduction of a potential infectious and inflammatory process after refractive surgery.
Acesso aberto (Open Access)
Laboratorial analyses of fungal keratitis in a University Service
(Conselho Brasileiro de Oftalmologia, 2000-02-01) Andrade, Alfredo José Muniz de [UNIFESP]; Vieira, Luís Antônio [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Yu, Maria Cecília Zorat [UNIFESP]; Gompertz, Olga Fischman [UNIFESP]; Freitas, Denise de [UNIFESP]; Souza, Luciene Barbosa de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Purpose: To present the frequency and type of identified fungi from infectious keratitis. Methods: Retrospective survey of the cases of mycotic keratitis in the period from 1995 to 1998, at the Laboratory of Ocular Microbiology of the Department of Ophthalmology of the Federal University of São Paulo. Description of the fungal isolations, analysis of the causative factors and relation to the number of infectious keratitis in the same period. Results/Conclusion: Mycotic keratitis was diagnosed in 61 (5.48%) of the 1,113 patients who presented ulcer of the cornea of infectious etiology, ranging from 3.4 to 9.25%, per year. Filamentous fungi were identified in 47 cases (77.04%) and yeasts in 14 (22.95%). Fusarium was the most frequent genus (50.82%), followed by Candida (22.95%) and Aspergillus (8.19%). Phaeosiaria sp, Phoma sp, Fonsecaea pedrosoi, Exserohilum rostratum, that are rare etiological fungal agents of keratitis, were also isolated.

Navegando por Palavras-chave "Keratitis"

Resultados por página

Opções de Ordenação