Navegando por Palavras-chave "Kazal inhibitor"

Agora exibindo 1 - 2 de 2
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    High-resolution structure of a Kazal-type serine protease inhibitor from the dengue vector Aedes aegypti
    (Int Union Crystallography, 2017) Torquato, Ricardo J. S. [UNIFESP]; Lu, Stephen [UNIFESP]; Martins, Nadia Helena; Tanaka, Aparecida S. [UNIFESP]; Barbosa Pereira, Pedro Jose
    Blood-feeding exoparasites are rich sources of protease inhibitors, and the mosquito Aedes aegypti, which is a vector of Dengue virus, Yellow fever virus, Chikungunya virus and Zika virus, is no exception. AaTI is a single-domain, noncanonical Kazal-type serine proteinase inhibitor from A. aegypti that recognizes both digestive trypsin-like serine proteinases and the central protease in blood clotting, thrombin, albeit with an affinity that is three orders of magnitude lower. Here, the 1.4 angstrom resolution crystal structure of AaTI is reported from extremely tightly packed crystals (similar to 22% solvent content), revealing the structural determinants for the observed inhibitory profile of this molecule.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Preliminary characterization of a Kazal-type serine protease inhibitor from Caiman crocodilus yacare plasma
    (Elsevier B.V., 1999-12-01) Araujo, Mariana S. [UNIFESP]; Nunes, Viviane A. [UNIFESP]; Gozzo, Andrezza Justino [UNIFESP]; Sampaio, Misako U. [UNIFESP]; Auerswald, Ennes; Ura, Nobuyuki; Shimamoto, Kazuaki; Sampaio, Claudio AM [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Innenstadt LMU Munchen; Sapporo Med Univ
    Blood serine protease inhibitors are becoming better understood and increasingly applied in blood clotting, cancer and other diseases. Reptiles are suitable models for blood coagulation and related processes, moreover, caiman is a good comparative model of a non-poisonous reptile. Recently, we reported the purification of a kininogen, the presence of proteases involved in blood clotting, and a serine protease inhibitor in Caiman crocodilus yacare plasma. in this paper, we described the partial sequence of an inhibitor (CcTI). the inhibitor is an 80-kDa protein, and it inactivates trypsin and chymotrypsin the hydrolysis of specific chromogenic substrates and in the degradation of gelatin. the inhibitor is member of Kazal-type inhibitor family and consists of several domains, its putative reactive: site is Arg-His. (C) 1999 Elsevier Science B.V. All rights reserved.