Navegando por Palavras-chave "Intestine"
Agora exibindo 1 - 10 de 10
Resultados por página
Opções de Ordenação
- ItemAcesso aberto (Open Access)Aspectos ultra-estruturais das vilosidades intestinais após o clampeamento do pedículo hepático de ratos(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2003-12-01) Camargo, Lázaro Manoel de; Evêncio Neto, Joaquim; Freitas, Silvio Henrique de; Simões, Manuel de Jesus [UNIFESP]; Gomes, Paulo de Oliveira [UNIFESP]; Sébe, Abrão Antônio; Universidade de Cuiabá Faculdade de Medicina Veterinária Departamento de Clínica; Universidade Federal Rural de Pernambuco Departamento de Morfologia e Fisiologia; Universidade Federal de São Paulo (UNIFESP); UNIC; UNIC Faculdade de MedicinaPURPOSE: To study the ultrastructural of rat intestinal villi (ileum) after total hepatic pedicle clumping for different period of time. METHODS: Forty male rats were divided into four groups of ten animals each. The Sham group was not being submitted to ischemia (control). E1, E2 and E3 group were submitted to 10, 20 and 30 minutes of ischemia, respectively. At the end of the experiment fragments of the ileum were taken off and examined by electromicroscope. RESULTS: Leucocyte infiltration, vascular dilation and electrotransparent areas were observed in intestinal villi of lamina propria of E1 and E2 groups. In reference to group E3 desquamation of epithelial layer, desquamation of intestinal villi and signs of apoptosis were observed. Hemorrhagic areas, dilated lymphatics and blood vessels and lots of leucocytes were observed in lamina propria. CONCLUSION: The clumping of hepatic pedicle provokes esplacnic congestion, time dependent, and to the 30 minutes were epithelial cell desquamation, apoptosis signs and hemorrhage.
- ItemAcesso aberto (Open Access)Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2004-10-01) D'acampora, Armando José [UNIFESP]; Ortellado, Daniel Knabben [UNIFESP]; Carvalho, Roberta O. M.; Serafim, João Daniel May; Farias, Débora Cadore De; Tramonte, Ricardo; Universidade Federal de São Paulo (UNIFESP); Universidade Federal de Santa Catarina Hospital Universitário; Hospital Regional de São José; Universidade Federal de Santa CatarinaPURPOSE: Observe the relation between small intestine's mucosal injury and bacterial translocation. METHODS: 50 adult female rats were distributed in 5 groups: 1. Control: intraperitoneal injection of Pseudomonas aeruginosa, 2. Drainage: intraperitoneal injection of P. aeruginosa and drainage of the abdominal cavity, after 6 hours, 3. Washed: intraperitoneal injection of P. aeruginosa and washing of the abdominal cavity, after 6 hours, 4. Washed + drainage: intraperitoneal injection of P. aeruginosa plus drainage and washing of the cavity, after 6 hours, 5. Normal: evaluation of the normal intestinal wall. After death, blood and peritoneal cultures were performed. Fragments of jejunum were processed histologically for morphometric measure of the total thickness of the jejujum's wall in comparison to the thickness of the mucosa + submucosa layer. RESULTS: In blood culture, there was growth of Pseudomonas aeruginosa and Escherichia coli in 90% and 52,5% of the animals, respectively. In the peritoneal culture, there was growth of P aeruginosa, E.coli and Klebsiella sp in 87,5%, 85% and 5% of the animals. About the histological analysis and the thickness of the mucosa + submucosa's layer, there was not significant alteration. CONCLUSION: Acute sepsis did not develop any thickness alteration in the small intestine's mucosa layer. Bacterial translocation can not be a direct consequence produced by mucosal intestinal injury.
- ItemAcesso aberto (Open Access)Avaliação do habito intestinal em mulheres atletas e sua relação com nível de hidratação e uso de suplemento(Inst Brasileiro Pesquisa & Ensino Fisiologia Exercicio-Ibpefex, 2016) Dall'Agnol, Tatyana [UNIFESP]; Araujo, Maíta Poli de [UNIFESP]; Laino, Fernanda [UNIFESP]; Parmigiano, Tathiana Rebizzi [UNIFESP]; Girão, Manoel João Batista Castello [UNIFESP]; Sartori, Marair Gracio Ferreira [UNIFESP]Objective: Constipation is the most common digestive complaint in the average population and occurs more frequently in women. Physical activity brings several health benefits, but it is still unknown the precise impact of high-performance exercises on regulating bowel habit. The purpose of the present study is to investigate bowel habit of female athletes and its relationship to the level of hydration and use of supplements through the Bristol Scale. Methods: The sample consisted of 45 high-performance female athletes, average age of 23 +/- 5 years. Bristol scale has been used to classify the stool consistency and questionnaires to assess the level of hydration and use of supplements. Weight, body circumferences and skin-folds measurements were taken. Results: It was found that 56% of athletes had stools considered normal, 28% had hard feces and 16% had soft stools. The use of supplementation (p = 0.03) and the hydration level (p = 0.04) were related to a higher incidence of soft stools in these athletes. Conclusion: High-performance exercise had a positive impact on bowel habit of athletes who had stool described as normal pattern. However, the use of supplements and the level of hydration changed the form of feces.
- ItemAcesso aberto (Open Access)Efeito da administração da polpa de Juçara (Euterpe edulis mart) em camundongos tratados com dieta hiperlipídica e hipercalórica sobre parâmetros metabólicos, inflamação e permeabilidade intestinal(Universidade Federal de São Paulo (UNIFESP), 2019-08-13) Silva, Fernanda Pinheiro Da [UNIFESP]; Oyama, Lila Missae [UNIFESP]; Rosso, Veridiana Vera de [UNIFESP]; http://lattes.cnpq.br/4938721558237749; Efeito da ingestão da polpa de juçara (Euterpe edulis Mart) em camundongos tratados com dieta hiperlipídica e hipercalórica sobre parâmetros metabólicos, inflamação, proteínas envolvidas na permeabilidade do intestino e microbiota bacteriana intestinal; http://lattes.cnpq.br/7125541171554727; http://lattes.cnpq.br/7044481306126169; Universidade Federal de São Paulo (UNIFESP)Obesity is multifactorial disease and one of factor is an ingestion of hypercaloric diet rich in fat and ultra-processed food and poor in micronutrients and fiber. The ingestion of fruits, vegetable and leguminous is emphasize by benefits and proprieties to promote health. In this sense, some food rich in bioactive compounds are studied to prove their capacity to decrease the deleterious metabolic effect promoted by refined sugar and fat rich-diet. The goal of this study was evaluated the effect of jucara (Euterpe edulis Mart) supplementation, fruit rich in fiber and bioactive compound, majority anthocyanin, on metabolism, inflammation, and also, the intestinal permeability and fecal bacterial microbiome characterization in obese mice induced by hypercaloric and hyperlipidic diet. Swiss mice were treated with hyperlipidic and hypercaloric diet supplemented or not with 0.5% and 2% of jucara pulp for sixteen weeks. Body composition and metabolic parameters were analyzed. Pro and anti-inflammatory cytokines were evaluated in different tissues. Proteins of TLR-4 pathway and tight junctions were quantified in the colon. Colon fecal bacterial microbiome characterization was done. The results showed that sixteen weeks treatment with hyperlipidic and hypercaloric diet was efficient to developing obesity in mice, changing both body composition and metabolism. On the other hand, the jucara supplementation did not alter statistically these parameters. We observed a physiological difference, decreasing the pro-inflammatory cytokines concentration in the epididymal adipose tissue depot, more evident in the 0.5% group compared to no supplemented one. Jucara supplementation decreased the pro-inflammatory cytokines and TLR-4 protein content and did not modify the tight junction proteins. The main composition of bacteria of the colon belongs to Firmicutes and Bacteroidetes filo and the jucara did not modify this pattern. The ratio Firmicutes/Bacteroidetes (F/B) was 0.87 in control group, indicating that Bacteroidetes abundance was higher than Firmicutes in lean animals. The opposite was observed in all hyperlipidic and hypercaloric diets, being the F/B ratio higher in the hypercaloric group (1.64) and lower in the groups treated with juçara pulp, at concentrations of 0.5 and 2% (1.17 and 1.58, respectively). The most expressive result was observed in the Firmicutes filo, specifically in the Erysipelotrichia class. The hyperlipidic diet increased the relative abundance compared to control group and jucara normalized to a control pattern. The hyperlipidic and hypercaloric diet supplemented with jucara for sixteen weeks did not modify the general metabolic aspects, but decreased the pro-inflammatory profile in the epididymal adipose tissue depot and colon. The exploratory analysis of the colon fecal bacterial microbiome was done and futures analysis of correlation with the metabolic and inflammatory parameter and the microbioma content could show the restrict relation between the intestine and the whole body metabolism.
- ItemAcesso aberto (Open Access)Effect of different periods of hyperbaric oxygen on ischemia-reperfusion injury of rat small bowel(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2008-02-01) Bertoletto, Paulo Roberto; Chaves, José Carlos; Fagundes, Anna Tereza Negrini; Simões, Ricardo Santos [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Simões, Manuel de Jesus [UNIFESP]; Fagundes, Djalma José [UNIFESP]; University of Grande Dourados; Santo Amaro University Medical School; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To determine whether hyperbaric oxygen (HBO) could effectively protect the small intestine mucosa against an ischemic insult, according to different periods of application. METHODS: The gut of 32 male rats was subjected to 60-min ischemia (clamping the mesenteric artery and vein); After they were further reperfused upon clamp opening during 60 min. Animal groups were as follows. GII = placed on HBO during the ischemia period; GIII = placed on HBO during reperfusion; GIV = treated with HBO throughout the ischemia-reperfusion period. Some animals (GI) did not receive HBO treatment at all and served as reference of ischemia-reperfusion injury (IR). HBO was carried out in a cylindrical acrylic chamber (2.0 ATA). Samples of small bowel were prepared for H.E staining for histological evaluations. RESULTS: The histological injury of mucosa was significantly less when HBO was administered during the ischemia period (17.6 ± 0.6) as compared with the IR (21.3 ± 1.8). HBO was not effective when applied during reperfusion (23.1 ± 2.1) or during the ischemia plus reperfusion period (18.7 ± 1.9). The thickness of the mucosa was preserved by HBO in ischemia (327.50 ± 30.23 µm) in comparison with the IR (172.79 ± 5.95 µm). In the periods of reperfusion (162.50 ± 6.05 µm) and ischemia plus reperfusion (296.49 ± 20.01 µm) the mucosa revealed a structural injury. CONCLUSION: Hyperbaric oxygen affects the ischemic insult of small bowel, being the favorable effect obtained when hyperbaric oxygen was administered early in the ischemic period.
- ItemAcesso aberto (Open Access)Effects of ischemic preconditioning associated to different preservation solutions in protecting the intestinal graft(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2011-10-01) Neves, José de Souza [UNIFESP]; Abrahão, Marcos de Souza [UNIFESP]; Salzedas-Netto, Alcides Augusto [UNIFESP]; Montero, Edna Frasson de Souza [UNIFESP]; Gonzalez, Adriano Miziara [UNIFESP]; University of Mato Grosso Department of Surgery; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To evaluate the effects of ischemic preconditioning (IPC) associate with different preservation solutions, in the protecting of gut. METHODS: Four groups of 14 rats underwent laparotomy and collecting 20 cm of ileum, for preservation, at 4ºC, in Belzer (Belz), Ringer (RL), Celsior (Cs) and Custodiol (Cust) solutions, for 24 hours. Prior to collection, half of the animals in each group were subjected to IPC. During preservation, in the periods of zero, 12, 18 and 24 hours, were conducted evaluating the degree of mucosal injury and dosage of malondialdehyde acid (MDA). RESULTS: In all periods the RL group, with and without IPC, presented MDA values higher than the Belz and Cs. The degree of mucosal injury in the non-ipc RLgroup with 12h preservation was higher than the others; with 18 and 24h, the RL and Cust had higher degrees of damage than Cs and Belz. With IPC, in all periods, the group Cs and Belz had lower degrees of injury. CONCLUSION: The Celsior and Belzer solutions had better protective effects on the gut and these effects were enhanced by IPC.
- ItemSomente MetadadadosInfluência da inflamação alérgica pulmonar na homeostasia intestinal(Universidade Federal de São Paulo (UNIFESP), 2020-09-02) Nascimento, Carolina Martins [UNIFESP]; Ferreira, Caroline Marcantonio [UNIFESP]; Universidade Federal de São PauloThe incidence and prevalence of chronic inflammatory diseases such as asthma and inflammatory bowel disease (IBD) are increasing worldwide, affecting mainly countries with western habits. Epidemiological data show that there is an important relationship between respiratory and intestinal diseases. Recent research indicates that inflammatory and immunological components of the mucosa are similar in the lung and intestine and that these similarities are one of the causes of the overlap of these diseases, the other causal factors include genetic and environmental factors. Knowing the interconnection between the pulmonary and intestinal mucosa and the overlap between respiratory and intestinal diseases, our aim was to investigate the influence of experimental pulmonary allergic inflammation induced by ovalbumin (OVA) on intestinal homeostasis. The experiments were carried out on A / J mice as it is one of the strains most susceptible to allergic airway inflammation and experimental colitis. The results obtained show that experimental allergic asthma induced by OVA caused shortening of the colon, increased myeloperoxidase in the distal colon mucosa, increased production of cytokines IL-4 and IL-13 in the colon, decreased production of neutral mucin in the mucosa of the colon and decreased integrity of the intestinal mucous layer. In conclusion, the data suggest that allergic pulmonary inflammation caused by OVA affects intestinal homeostasis.
- ItemSomente MetadadadosIntestinal microbiota and allergic diseases: A systematic review(Elsevier Doyma Sl, 2016) Melli, L. C. F. L. [UNIFESP]; do Carmo-Rodrigues, M. S.; Araujo-Filho, H. B. [UNIFESP]; Sole, D. [UNIFESP]; de Morais, M. B. [UNIFESP]Evidence suggests that possible imbalances in intestinal microbiota composition may be implicated in the occurrence of allergic diseases. Although several studies published until 2006 indicated a correlation between microbiota composition and allergic symptoms, it has not been possible to distinguish protective microorganisms from those associated with increased risk of allergic diseases. Therefore, the objective of this study was to review the studies published since 2007 that address the intestinal microbiota in allergic diseases. Twenty-one studies were identified after excluding those that performed a clinical intervention before stool collection. In the early microbiota of children who later developed allergies, lower bacterial diversity was observed, with a predominance of Firmicutes; a higher count of Bacteroidaceae; a higher prevalence of the anaerobic bacteria Bacteroides fragilis, Escherichia coli, Clostridium difficile, Bifidobacterium catenulatum, Bifidobacterium bifidum, and Bifidobacterium longum; and a lower prevalence of Bifidobacterium adolescentis, B. bifidum, and Lactobacillus. In the microbiota of allergic children whose intestinal microbiota was assessed at the onset of allergic symptoms, there was a higher count of Bacteroides; a lower count of Akkermansia muciniphila, Faecalibacterium prausnitzii, and Clostridium; a higher prevalence of B. adolescentis; a lower prevalence of B. catenulatum and Staphylococcus aureus; and a lower bacterial diversity. (C) 2014 SEICAP. Published by Elsevier Espana, S.L.U. All rights reserved.
- ItemSomente MetadadadosMesenchymal stromal cells modulate gut inflammation in experimental colitis(Springer Basel Ag, 2018) de Aguiar, Cristhiane Favero; Castoldi, Angela; Andrade-Oliveira, Vinicius; Ignacio, Aline; da Cunha, Flavia Franco [UNIFESP]; Ferreira Felizardo, Raphael Jose [UNIFESP]; Bassi, Enio Jose; Saraiva Camara, Niels Olsen [UNIFESP]; de Almeida, Danilo Candido [UNIFESP]Inflammatory bowel diseases (IBDs) affect millions of people worldwide and their frequencies in developed countries have increased since the twentieth century. In this context, there is an intensive search for therapies that modulate inflammation and provide tissue regeneration in IBDs. Recently, the immunomodulatory activity of adipose tissue-derived mesenchymal stromal cells (ADMSCs) has been demonstrated to play an important role on several immune cells in different conditions of inflammatory and autoimmune diseases. In this study, we explored the immunomodulatory potential of ADMSC in a classical model of DSS-induced colitis. First, we found that treatment of mice with ADMSC ameliorated the severity of DSS-induced colitis, reducing colitis pathological score and preventing colon shortening. Moreover, a prominent reduction of pro-inflammatory cytokines levels (i.e., IFN-gamma, TNF-alpha, IL-6 and MCP-1) was observed in the colon of animals treated with ADMSC. We also observed a significant reduction in the frequencies of macrophages (F4/80(+)CD11b(+)) and dendritic cells (CD11c(+)CD103(+)) in the intestinal lamina propria of ADMSC-treated mice. Finally, we detected the up-regulation of immunoregulatory-associated molecules in intestine of mice treated with ADMSCs (i.e., elevated arginase-1 and IL-10). Thus, this present study demonstrated that ADMSC modulates the overall gut inflammation (cell activation and recruitment) in experimental colitis, providing support to the further development of new strategies in the treatment of intestinal diseases.
- ItemAcesso aberto (Open Access)Small bowel transplantation in outbred rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2011-12-01) Waisberg, Daniel Reis; Lee, André Dong Wong; Santos, Rafael Miyashiro Nunes dos; Mory, Eduardo Kenji; Costa, Anderson Lino; Montero, Edna Frasson de Souza [UNIFESP]; Chaib, Eleazar; D'albuquerque, Luis Augusto Carneiro; Galvao, Flavio Henrique Ferreira; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)PURPOSE: To investigate the clinical evolution of orthotopic small bowel transplantation in outbred rats. METHODS: Seventy-two outbred Wistar rats weighting from 250 to 300g were used as donor and recipient in 36 consecutives ortothopic small intestine transplantation without immunosuppression. The graft was transplanted into the recipient using end-to-side aortic and portacaval microvascular anastomosis. Procedure duration, animal clinical course and survival were evaluated. Survival shorter than four days was considered technical failure. Recipients were sacrificed with signs of severe graft rejection or survival longer than 120 days. Necropsies were performed in all recipients to access histopathological changes in the graft. RESULTS: Median time for the procedure was 107 minutes. Six recipients (16.7%) presented technical failure. Twenty-seven recipients were sacrificed due to rejection, being nineteen (52.7%) between 7th and 15th postoperative day and eight (22.2%) between 34th and 47th postoperative day. Graft histology confirmed severe acute cellular rejection in those recipients. Uneventful evolution and survival longer than 120 days without rejection were observed in three recipients (8.3%). CONCLUSION: Intestinal transplantation in outbred rats without immunosuppressant regiment accomplishes variable clinical evolution.