Navegando por Palavras-chave "Interferon-gamma"
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- ItemSomente MetadadadosEvaluation of periodontitis in hospital outpatients with major depressive disorder. A focus on gingival and circulating cytokines(Academic Press Inc Elsevier Science, 2016) de Oliveira Solis, Ana Cristina; Marques, Andrea Horvath; Dominguez, Wagner Vasques; de Almeida Prado, Euthymia Brandao [UNIFESP]; Pannuti, Claudio Mendes; Moreira Lotufo, Roberto Fraga; Lotufo-Neto, FranciscoAn imbalance in stimulated cytokine production is associated with the etiopathogenesis of numerous diseases such as major depressive disorder (MDD) and periodontal disease. Increased cytokine levels have been reported in the gingival crevicular fluid (GCF) of patients with MDD. Thirty-six outpatients with MDD participated in this study. Each outpatient was age-matched (+/- 3 years) with a healthy control (n = 36). The patients were controlled for race and smoking habits. Unstimulated and stimulated interleukin 6 (IL-6), interleukin 1 beta (IL-1 beta), and interferon-gamma (INF-gamma) production in whole blood culture (WBC) and IL-6 and IL-1 beta levels in the GCF were evaluated. Circulating levels of IL-6 and IL-1 beta (unstimulated) as well as GCF IL-1 beta were modestly lower in MDD patients, compared to the levels in age-matched controls (Mann-Whitney, p = 0.002, 0.0075, ANCOVA, p = 0.025, respectively). In the unstimulated group, there was no correlation between the levels of circulating IL-6 and GCF IL-6 (r = 0.07, p = 0.67), and between the levels of circulating IL-1 beta and the IL-1 beta level in the CGF (r = -0.08, p = 0.63). In the LPS stimulation group, there was no correlation between the levels of circulating levels of IL-6 and GCF IL-6 (r = 0.02, p= 0.91) or between the circulating IL-1 beta and GCF IL-1 beta (r = 0.13, p= 0.42). We observed modest immunosuppression in MDD patients (evaluated by no stimulation whole blood culture [WBC]), especially in patients with melancholic depression, chronic depression, and severe depression. (C) 2015 Elsevier Inc. All rights reserved.
- ItemAcesso aberto (Open Access)Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection(Associação Brasileira de Divulgação Científica, 2001-06-01) Shulzhenko, Natalia [UNIFESP]; Morgun, Andrey [UNIFESP]; Franco, Marcello Fabiano de [UNIFESP]; Souza, Marcia Marcelino de [UNIFESP]; Almeida, Dirceu Rodrigues de [UNIFESP]; Diniz, Rosiane Viana Zuza [UNIFESP]; Carvalho, Antonio Carlos [UNIFESP]; Pacheco-Silva, Alvaro [UNIFESP]; Gerbase-Lima, Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The purpose of the present study was to investigate the expression (mRNA) of CD40 ligand (CD40L), interferon-gamma (IFN-gamma) and Fas ligand (FasL) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection. The mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction method in 39 samples of endomyocardial biopsies obtained from 10 adult cardiac transplant recipients within the first six months after transplantation. Biopsies with ongoing acute rejection showed significantly higher CD40L, IFN-gamma and FasL mRNA expression than biopsies without rejection. The median values of mRNA expression in biopsies with and without rejection were 0.116 and zero for CD40L (P<0.003), 0.080 and zero for IFN-gamma (P<0.0009), and 0.156 and zero for FasL (P<0.002), respectively. In addition, the levels of IFN-gamma mRNA were significantly increased 7 to 15 days before the appearance of histological evidence of rejection (median of 0.086 in pre-rejection biopsies), i.e., they presented a predictive value. This study provides further evidence of heightened expression of immune activation genes during rejection and shows that some of these markers may present predictive value for the occurrence of acute rejection.
- ItemSomente MetadadadosIncreased interferon-mediated immunity following in vitro and in vivo Modafinil treatment on peripheral immune cells(Pergamon-Elsevier Science Ltd, 2018) Zager, Adriano; Brandao, Wesley Nogueira; Margatho, Rafael Oliveira; Gimenes Cruz, Daniel Sanzio; Peron, Jean Pierre; Tufik, Sergio [UNIFESP]; Andersen, Monica Levy [UNIFESP]; Moresco, Monica; Pizza, Fabio; Plazzi, Giuseppe; Kornum, Birgitte Rahbek; Palermo-Neto, JoaoThe wake-promoting drug Modafinil has been used for treatment of sleep disorders, such as Narcolepsy, excessive daytime sleepiness and sleep apnea, due to its stimulant action. Despite the known effect of Modafinil on brain neurochemistry, particularly on brain dopamine system, recent evidence support an immunomodulatory role for Modafinil treatment in neuroinflammatory models. Here, we aimed to study the effects of in vitro and in vivo Modafinil treatment on activation, proliferation, cell viability, and cytokine production by immune cells in splenocytes culture from mice. The results show that in vitro treatment with Modafinil increased Interferon (IFN)-gamma, Interleukin (IL)-2 and IL-17 production and CD25 expression by T cells. In turn, in vivo Modafinil treatment enhanced splenocyte production of IFN-gamma, IL-6 and tumor necrosis factor (TNF), and increased the number of IFN-gamma producing cells. Next, we addressed the translational value of the observed effects by testing PBMCs from Narcolepsy type 1 patients that underwent Modafinil treatment. We reported increased number of IFN-gamma producing cells in PBMCs from Narcolepsy type 1 patients following continuous Modafinil treatment, corroborating our animal data. Taken together, our results show, for the first time, a pro-inflammatory action of Modafinil, particularly on IFN-mediated immunity, in mice and in patients with Narcolepsy type 1. The study suggests a novel effect of this drug treatment, which should be taken into consideration when given concomitantly with an ongoing inflammatory or autoimmune process.
- ItemSomente MetadadadosProdução de interferom-gama, interleucina-10 e fator de necrose de tumor-alfa por células mononucleares do sangue periférico de indivíduos infectados pelo HIV-1(Universidade Federal de São Paulo (UNIFESP), 1999) Turcato Junior, Gilberto [UNIFESP]; Salomão, Reinaldo [UNIFESP]Complexa rede de citocinas participa, direta ou indiretamente, da regulacao da replicacao viral e na inducao de apoptose de linfocitos CD4+ durante a infeccao pelo HIV-1. Este estudo compara a producao in vitro de TNF-a, IL-10 e IFN-g por celulas mononucleares do sangue periferico (CMSP) obtidas de dois grupos de individuos: 23 infectados pelo HIV-1 assintomaticos e 25 controles sadios. Avalia ainda a possivel correlacao da producao destas citocinas com a contagem de linfocitos T CD4+ e carga viral plasmatica no grupo de individuos infectados pelo HIV-1. Os individuos infectados pelo HIV-1 eram assintomaticos, sem historia atual ou pregressa de manifestacoes oportunistas e sem tratamento anti-retroviral anterior. A contagem de linfocitos T CD4+ foi realizada por citometria de fluxo; a determinacao quantitativa do RNA do HIV-1 realizada pelo metodo NASBRA. As CMSP foram corrigidas para a contagem de 2,0 x 106 em meio RPMI 1640 suplementada com 10 por cento de soro autologo e foram incubadas sem estimulacao (producao constitutiva) ou com estimulo de LPS (para induzir a TNF-a) ou de PHA (para inducao de IL-10 ou INF-g). Foi utilizada ainda, estimulacao especifica atraves de antigenos de HIV-1 pre-adsorvidos em placas. Para a comparacao da producao de citocinas entre individuos infectados e controles foi utilizado o teste de Mann-Whitney. O teste de correlacao de Spearman foi utilizado para as correlacoes de duas variaveis. Foi considerado aceitavel um risco menor que 5 por cento de cometer o erro tipo I (p<0,05). A producao constitutiva de TNF-a (p=0,068) e IL (p=0,002) foi maior nos infectados que nos controles. Nao houve diferenca entre os dois grupos com relacao a producao constitutiva de INF-g. Sob estimulacao com LPS para producao de TNF-a, nao houve diferenca entre os grupos na producao de TNF-a. Sob estimulacao com PHA, as CMPS de individuos infectados produziram menores niveis de IL-10 (p=0,018) e INF-g (p=0,059) que os controles, traduzindo algum deficit funcional das CMSP para responder a PHA. Em relacao ao estimulo com antigenos do HIV-1 adsorvidos em placa, houve maior producao de TNF-a (p=0,00003) e de IL-10 (p=0,0008) entre individuos infectados pelo HIV-1. Nao houve diferenca na determinacao dos niveis de INF-g apos estimulacao com antigenos de HIV-1. Nao foi observada correlacao significante entre a contagem de linfocitos T CD4+ e os niveis produzidos de citocinas por CMSP, com ou sem estimulacao. Quando analisada a correlacao entre a ...(au)
- ItemSomente MetadadadosProtective role of NKT cells and macrophage M2-driven phenotype in bleomycin-induced pulmonary fibrosis(Springer Basel Ag, 2018) Grabarz, Felipe; Aguiar, Cristhiane Favero; Correa-Costa, Matheus; Braga, Tarcio Teodoro; Hyane, Meire Ioshie [UNIFESP]; Andrade-Oliveira, Vinicius; Landgraf, Maristella de Almeida [UNIFESP]; Câmara, Niels Olsen Saraiva [UNIFESP]Pulmonary fibrosis is a result of an abnormal wound healing in lung tissue triggered by an excessive accumulation of extracellular matrix proteins, loss of tissue elasticity, and debit of ventilatory function. NKT cells are a major source of Th1 and Th2 cytokines and may be crucial in the polarization of M1/M2 macrophages in pulmonary fibrogenesis. Although there appears to be constant scientific progress in that field, pulmonary fibrosis still exhibits no current cure. From these facts, we hypothesized that NKT cells could influence the development of pulmonary fibrosis via modulation of macrophage activation. Wild type (WT) and NKT type I cell-deficient mice (J alpha 18(-/-)) were subjected to the protocol of bleomycin-induced pulmonary fibrosis with or without treatment with NKT cell agonists alpha-galactosylceramide and sulfatide. The participation of different cell populations, collagen deposition, and protein levels of different cytokines involved in inflammation and fibrosis was evaluated. The results indicate a benign role of NKT cells in J alpha 18(-/-) mice and in wild-type alpha-galactosylceramide-sulfatide-treated groups. These animals presented lower levels of collagen deposition, fibrogenic molecules such as TGF-beta and vimentin and improved survival rates. In contrast, WT mice developed a Th2-driven response augmenting IL-4, 5, and 13 protein synthesis and increased collagen deposition. Furthermore, the arginase-1 metabolic pathway was downregulated in wild-type NKT-activated and knockout mice indicating lower activity of M2 macrophages in lung tissue. Hence, our data suggest that NKT cells play a protective role in this experimental model by down modulating the Th2 milieu, inhibiting M2 polarization and finally preventing fibrosis.
- ItemSomente MetadadadosResposta imune celular e humoral dirigida contra antígenos do Clostridium tetani e do vírus do sarampo(Universidade Federal de São Paulo (UNIFESP), 2008) Viana, Patrícia Oliveira [UNIFESP]; Moraes-Pinto, Maria Isabel de [UNIFESP]Objetivo: Padronizar um método para avaliação in vitro da resposta imune celular contra o Clostridium tetani e o vírus do sarampo e avaliar a resposta imune celular e humoral em indivíduos com diferentes tipos de exposição a esses antígenos. Métodos: Foram avaliados 19 adultos sadios vacinados contra sarampo (SV); 20 adultos sadios com história de infecção pelo vírus do sarampo (SD); 20 indivíduos sadios com vacinação contra o tétano em dia (TD); 20 indivíduos sadios vacinados contra o tétano, mas sem reforço nos últimos dez anos (TN). A resposta imune celular foi realizada através da marcação de IFNy intracelular em células TCD4+ e TCD8+ por citometria de fluxo, após estimulação de sangue total e células monomorfonucleares a fresco e após congelamento com os antígenos dosarampo e do tétano. A avaliação da resposta imune humoral foi realizada através de ELlSA. Dez indivíduos de cada grupo receberam uma dose adicional da vacina do sarampo ou tétano e tiveram sua resposta imune celular e humoral avaliadas pré- e pós-vacina. Resultados: Na avaliação da resposta celular aos antígenos do sarampo e do tétano, o método utilizando sangue total foi capaz de detectar uma maior porcentagem de células TCD4+ e TCD8+produzindo IFNy que o método utilizando células monomorfonucleares a fresco e após congelamento. Entre os grupos SV e SD não foi observada diferença na porcentagem de IFNy produzido pelas células TCD4+ e TCD8+ (CD4- SV: 1,15% versus SD: 1,38%, teste t, p=0,84) (CD8- SV: 2,40% versus SD: 1,95%, teste t, p=0,39). Todos os indivíduos apresentaram níveis protetores de anticorpos contra o sarampo, mas o grupo SD apresentou níveis mais elevados...(au).