Navegando por Palavras-chave "IL-12"

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    Critical involvement of Th1-related cytokines in renal injuries induced by ischemia and reperfusion
    (Elsevier B.V., 2009-06-01) Paiva, Vanessa Nunes de [UNIFESP]; Monteiro, Rebecca M. M. [UNIFESP]; Marques, Vilmar de Paiva; Cenedeze, Marcos Antonio [UNIFESP]; Teixeira, Vicente de P. A.; Reis, Marlene A. dos; Pacheco-Silva, Alvaro [UNIFESP]; Camara, Niels O. S. [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Hosp Israelita Albert Einstein; Univ Fed Triangulo Mineiro
    Renal ischemia and reperfusion injury (IRI) is considered an inflammatory syndrome. To move forward in its pathogenesis, we exploited the role of several cytokines on renal damages triggered by IRI. Specifically to evaluate the role of Th1 immune profile in this system, IL-12, IFN-gamma, and IFN-gamma/IL-12 deficient (KO) mice on C57BL/6 background and their controls were subjected to IRI. in each group, blood and kidney samples were harvested. Renal function was evaluated by serum creatinine and renal morphometric analyses. Gene expression of IL-6 and HO-1 were also investigated by Q-PCR. IFN-gamma KO animals presented the highest impairment in renal function compared to controls. Conversely, IL-12 KO animals were absolutely protected and, in a lesser extent, IFN-gamma/IL-12 KO double knockout was also protected from IRI. Gene expression analyses showed higher expression of HO-1, a cytoprotective gene, and IL-6, a pro-inflammatory cytokine, in IFN-gamma deficient animals subjected to IRI. Our results confirm that Th1 related cytokines such as IL-12 and IFN-gamma are critically involved in renal ischemia and reperfusion injury. (C) 2008 Elsevier B.V. All rights reserved.
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    Differential inhibitory mechanism of cyclic AMP on TNF-alpha and IL-12 synthesis by macrophages exposed to microbial stimuli
    (Stockton Press, 1999-07-01) Procópio, Daniela O. [UNIFESP]; Teixeira, Mauro M.; Camargo, Maristela M.; Travassos, Luiz R.; Ferguson, Michael AJ; Almeida, Igor C.; Gazzinelli, Ricardo T.; Universidade Federal de Minas Gerais (UFMG); FIOCRUZ; Universidade Federal de São Paulo (UNIFESP); Univ Dundee
    1 Microbial stimuli such as bacterial lipopolysaccharide (LPS) or glycosylphosphatidylinositol-mucins derived from Trypanosoma cruzi trypomastigotes (tGPI-mucins) are effective stimulators of the synthesis of cytokines by macrophages. Here, we evaluated the ability of cyclic AMP mimetic or elevating agents to modulate TNF-alpha and IL-12 synthesis by murine inflammatory macrophages.2 Cholera Toxin (ChTx) inhibited tGPI-mucins (2.5 nM) or LPS (100 ng ml(-1)) induced TNF-alpha and IL-12(p40) synthesis in a concentration-dependent manner. Similarly, the cyclic AMP mimetics, 8-bromo cyclic AMP or dibutyryl cyclic AMP, or prostaglandin (PG) E-2 inhibited the synthesis of both cytokines by macrophages exposed to microbial stimuli.3 the protein kinase A inhibitor H-89 partially reversed the inhibitory effects of dibutyryl cyclic AMP and PGE(2) on both IL-12(p40) and TNF-alpha synthesis.4 Pretreatment of macrophages with dibutyryl cyclic AMP or ChTx augmented the synthesis of IL-10 triggered by microbial products. Elevation of cyclic AMP inhibited the synthesis of TNF-alpha, but not IL-12(p40), by inflammatory macrophages from IL-10 knockout mice.5 Kinetic studies showed that synthesis of both TNF-alpha and IL-10 peaked at 8 h and IL-12 at 24 h after stimulation with microbial stimuli.6 Together, our findings favour the hypothesis that the cyclic AMP inhibitory activity on IL-12(p40) but not on TNF-alpha synthesis is dependent on de novo protein synthesis, most likely involving IL-10, by macrophages stimulated with microbial products. Accordingly, dibutyryl cyclic AMP inhibited IL-12(p40) synthesis only when added before or at the same time of the stimuli. in contrast, the effect of this cyclic AMP analogue on TNF-alpha synthesis was protracted and observed even 2 h after the addition of the stimuli.
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    Estudo do efeito da manipulação da rede de citocinas sobre o padrão de sono e desempenho de camundongos na tarefa de esquiva inibitória de múltiplas tentativas
    (Universidade Federal de São Paulo (UNIFESP), 2016-03-31) Esumi, Lia Assae [UNIFESP]; Hipólide, Débora Cristina [UNIFESP]; http://lattes.cnpq.br/6303382961871353; http://lattes.cnpq.br/5665138706228728; Universidade Federal de São Paulo (UNIFESP)
    Among the many functions already related to sleep, the relationship of this phenomenon with the memory processing is probably one of the most controversial issues in neuroscience. In attempt to obtain further clarification on this issue, this study aimed to modulate the sleep pattern of mice with the objective of analyze possible changes in memory. To modulate the sleep pattern we used a class of molecules that endogenously participates in sleep regulation ? the cytokines. The anti-inflammatory cytokine interleukin-10 (IL-10) and the pro-inflammatory cytokine IL-12 were administered intraperitoneally into Swiss mice in attempt to modulate the inflammatory profile. There were three purposes: (1) verify the effect of IL-10 and IL-12 treatments in the sleep pattern, (2) verify the effect of IL-10 and IL-12 treatments in the sleep pattern along with the performance in a memory task, and (3) identify possible correlations between sleep pattern and performance on the memory task. To evaluate the sleep pattern, the animals underwent a surgery to implant electrodes for eletrocorticogram and electromyogram recordings. Memory was evaluated through the multiple-trial inhibitory avoidance (MTIA) task. The MTIA task training was performed immediately after treatments, and animals were tested 24h later. Alone, IL-10 and IL-12 treatments did not induce changes in sleep pattern. However, when treatments were conducted immediately before the MTIA task training, the IL-12 group showed decrease in the number of wake, slow-wave sleep (SWS), and paradoxical sleep (PS) episodes; increase in the mean duration of wake episodes; and decrease in the mean duration of PS episodes. Such sleep/wake changes were accompanied by performance improvement of IL-12 group in the EIMT task test. The correlations between the performance on MTIA task and the sleep/wake parameters in the post-training period indicated the contribution of SWS in the first three hours after training, followed by the greater contribuition of the complete cycle, wake? SWS? PS, for the best performance in the EIMT task. We suggest that the superior performance of the IL-12 group is the result of optimization of the memory consolidation process through changes in sleep patterns induced by IL-12 treatment.
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    Immunomodulatory activities associated with beta-glucan derived from Saccharomyces cerevisiae
    (Acad Sciences Czech Republic, Inst Physiology, 2005-01-01) Pelizon, Ana Claudia; Kaneno, Ramon; Soares, Angela Maria Victoriano de Campos [UNIFESP]; Meira, Domingos Alves [UNIFESP]; Sartori, Alexandrina; Universidade Federal de São Paulo (UNIFESP)
    In this study we investigated the effect of beta-glucan derived from Saccharomyces cerevisiae on fungicidal activity, cytokine production and natural killer activity. Spleen and peritoneal cells from female C57BL/6 mice, previously injected (24 or 48 h) with 20 or 100 mu g of glucan by i.p. route, were assayed. In vivo mu-glucan administration primed spleen cells for a higher production of IL-12 and TNF-alpha when S. aureus was used as a stimulus. In addition, beta-glucan increased NK spleen cells activity against YAC target cells. Some immunomodulatory activities not yet described for beta-glucan were observed in this work.
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    Interleukin-12 protects mice against disseminated infection caused by Paracoccidioides brasiliensis but enhances pulmonary inflammation
    (Elsevier B.V., 2002-05-01) Arruda, C.; Franco, M. F.; Kashino, S. S.; Nascimento, FRF; Fazioli, R. D.; Vaz, CAC; Russo, M.; Calich, VLG; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Insat Adolpho Lutz
    Paracoccidioides brasiliensis is a facultative, intracellular pathogen causing the most important deep mycosis in Latin America. As the production of IFN-gamma and induction of cell-mediated immunity to P. brasiliensis is of critical importance in host defense, the immunotherapeutic effect of exogenous IL-12 administration was studied in a murine model of susceptibility to pulmonary infection. rIL-12 treatment led to a less disseminated disease, as confirmed by decreased fungal loads in liver and spleen. Administration of rIL-12 did not affect fungal growth in the lungs, although it did induce an augmented pulmonary mononuclear cell inflammation. IL-12 treatment induced an early (week 1) increase in pulmonary IFN-gamma, but decreased cytokine and specific antibody (IgG1 and IgG3) production at week 8 after infection. These results show that IL-12 administration induces a less severe infection, but the high inflammatory response detected in the lungs precludes its possible use as a new therapeutic tool for severe paracoccidioidomycosis. (C) 2002 Elsevier Science (USA).