Navegando por Palavras-chave "Hyaluronan"
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- ItemSomente MetadadadosConcentration of glycosaminoglycan in ovariectomized mice uterus after treatment with ovarian steroids(Hindawi Publishing Corp, 2016) Maioral, Gabriela C. C. C. [UNIFESP]; Gomes, Regina Celia T. [UNIFESP]; Verna, Carina [UNIFESP]; Simoes, Manuel de J. [UNIFESP]; Nader, Helena B. [UNIFESP]; Simoes, Ricardo S.; Baracat, Edmund C.; Soares-, Jose Maria, Jr.The aim of this study was to evaluate the amount of non-and sulfated glycosaminoglycans in the ovariectomized mice uterus, after treatment with ovarian steroids. For this purpose, 50 adult female mice were divided into five groups with 10 animals/each: control group: CG (ovary intact), and ovariectomized groups: OG (vehicle), EG (estradiol), PG (progesterone) and EPG (estradiol combined to progesterone). The treatments started 30 days after ovariectomy. All the animals were treated for 50 consecutive days. These hormones were administered in a sterile oily solution via gavage. Twenty-four hours after the last treatment, all animals were euthanized, removing the uterine horn for biochemical analyses. To quantify, the hyaluronic acid (HA) used ELISA-like fluorometric assay, and the sulfated glycosaminoglycans (GAGs) used agarose gel electrophoresis. The amount of HA was significantly higher in the group treated with progesterone (PG) compared to the others groups (p < 0.05), and in the group treated with estradiol (EG), the amount of chondroitin/dermatan sulfate was significantly higher compared to the others groups (p < 0.05), and in the group treated with progesterone (PG), the amount of heparan sulfate was significantly lower compared to the others groups, except to control group (p < 0.05). Our results showed that the estroprogestative therapy after long time (50 days) profoundly affected the amount of glycosaminoglycans in uterine. These changes may be indicative of uterine pathology such as the development of tumor.
- ItemSomente MetadadadosHyaluronan involvement in the changes of mouse interpubic tissue during late pregnancy and post-partum(Elsevier B.V., 2008-08-01) Garcia, Eduardo A.; Veridiano, Adriano M.; Martins, Joao R. M. [UNIFESP]; Nader, Helena B. [UNIFESP]; Pinheiro, M. C. [UNIFESP]; Joazeiro, Paulo P.; Toledo, Olga M. S. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Campinas (UNICAMP)The present work quantifies hyaluronan (HA) during the late pregnancy and post-partum in order to provide a better understanding of the role of HA in the adaptations that occur in the pubic symphysis during this period. HA was quantified in situ (histochemically) and in interpubic tissue extracts by fluorimetric assay. Samples were taken from virgin mice and from pregnant animals at various stages of pregnancy: 12th- 18th days into pregnancy, the day of delivery (D19) and the 3rd and 5th day post-partum. the quantitative fluorimetric analysis indicated a gradual increase of HA in the interpubic tissue throughout late pregnancy (2.4-4.6 mu g/mg dry weight). This was followed by a decrease beginning on D19 (12.4 mu g/mg), reaching close to virgin levels (2.2 mu g/mg) on the 5th day post-partum. the same optical density changes could be seen in the HA staining. Furthermore, the histochemical analysis demonstrated the presence of HA both in the extracellular matrix of the tissue and within its cells. Such results indicate that the extracellular presence of HA may contribute to the transformation of the symphysis into a flexible structure. in addition, HA's intracellular presence (until the 18th day of pregnancy) may contribute to cellular proliferation. Finally, during parturition and on the 5th day post-partum, HA may contribute to the maintenance of the myofibroblastic phenotype of ligament cells, aiding the ligament involution after parturition. (c) 2008 International Federation for Cell Biology. Published by Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosIonic and biochemical characterization of bovine intervertebral disk(Taylor & francis inc, 2016) Martins, Delio Eulalio [UNIFESP]; Medeiros, Valquiria Pereira; Demerov, Gabriela Feitosa [UNIFESP]; Accardo, Camila Melo [UNIFESP]; Paredes-Gamero, Edgar Julian [UNIFESP]; Wajchenberg, Marcelo [UNIFESP]; Reginato, Rejane Daniele [UNIFESP]; Nader, Helena Bonciani [UNIFESP]; Puertas, Eduardo Barros [UNIFESP]; Faloppa, Flavio [UNIFESP]Introduction: Intervertebral disks have been associated with low back pain, and many therapies have been proposed for its treatment. The cellular and molecular knowledge of intervertebral disks composition and precise methods to quantify disk components are important for any type of proposed therapy. Thus, the aim of this study was to correlate glycosaminoglycans presence with the quantitation of cells, ions and collagen fiber distributions in different intervertebral disk sections. Methods: In total, 14 intervertebral disks were used from cattle. All of the disks were dehydrated, separated in seven sections and digested in sodium-free papain buffer. Glycosaminoglycan measurements were performed in the samples according to agarose electrophoresis method
- ItemAcesso aberto (Open Access)Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease(Associação Brasileira de Divulgação Científica, 2005-05-01) Santos, V.n. dos [UNIFESP]; Leite-Mór, Marilisa Moraes Barros [UNIFESP]; Kondo, Mario [UNIFESP]; Martins, J.r. [UNIFESP]; Nader, Helena Bonciani [UNIFESP]; Lanzoni, Valeria Pereira [UNIFESP]; Parise, Edison Roberto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Hepatic fibrosis in patients with non-alcoholic fatty liver disease is associated with progression of the disease. In the present study, we analyzed the discriminative ability of serum laminin, type IV collagen and hyaluronan levels to predict the presence of fibrosis in these patients. In this preliminary report, we studied 30 overweight patients divided into two groups according to the absence (group I, N = 19) or presence (group II, N = 11) of fibrosis in a liver biopsy. Triglycerides, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidade, hyaluronan (noncompetitive fluoroassay), type IV collagen, and laminin (ELISA) were determined. Group II presented significantly higher mean laminin, hyaluronan, type IV collagen, and aspartate aminotransferase values, which were due to the correlation between these parameters and the stage of fibrosis in the biopsy (Spearman's correlation coefficient, rS = 0.65, 0.62, 0.53, and 0.49, respectively). Analysis of the ROC curve showed that laminin values >282 ng/ml were those with the best diagnostic performance, with 87% accuracy. Association of laminin with type IV collagen showed improvement in the positive predictive value (100%), but with reduction in diagnostic sensitivity (64%). When compared with the criteria of Ratziu et al. [Gastroenterology (2000) 118: 1117-1123] for the diagnosis of septal fibrosis, laminin values presented a better diagnostic accuracy (83 vs 70%). Determination of extracellular matrix components in serum, especially of laminin, may identify patients with non-alcoholic fatty liver disease and fibrosis and these components may be used as indicators for liver biopsy in these patients.