Navegando por Palavras-chave "Humor aquoso"
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- ItemAcesso aberto (Open Access)Aqueous humor concentrations of topical fluoroquinolones alone or in combination with a steroid(Consel Brasil Oftalmologia, 2017) Rodrigues Gomes, Rachel Lopes [UNIFESP]; Viana, Rodrigo Galvao; Soares Melo, Luiz Alberto, Jr. [UNIFESP]; Cruz, Alessandro Carvalho [UNIFESP]; de Souza Lima Filho, Acacio Alves [UNIFESP]; Suenaga, Eunice Mayumi [UNIFESP]; Campos, Mauro [UNIFESP]Purpose: To compare the aqueous humor (AH) concentrations of moxifloxacin 0.5% and gatifloxacin 0.3% solutions alone or when treatment was combined with steroids, and to correlate these concentrations with the minimum inhibitory concentrations (MIC) for the most common endophthalmitis-causing organisms. Methods: Patients undergoing phacoemulsification were enrolled to receive one drop of one of the following solutions: moxifloxacin (G1), moxifloxacin + dexamethasone (G2), gatifloxacin (G3), or gatifloxacin + c (G4), every 15 min, 1h before surgery. AH samples were collected before surgery and analyzed using HPLC-tandem mass spectrometry. Results: The mean antibiotic concentrations in the AH were: G1= 1280.8 ng/mL, G2= 1644.3 ng/mL, G3= 433.7 ng/mL and G4= 308.1 ng/mL. The mean concentrations statistically differed between G1 and G2 (p=0.01), and G3 and G4 (p=0.008). All samples achieved the MIC for Staphylococcus epidermidis 100% of the samples from G1 and G2, and 97% from G3 and G4 reached the MIC for fluoroquinolone-sensitive Staphylococcus aureus 100% of the samples from G1 and G2, 88% from G3, and 72% from G4 reached the MIC for enterococci (p<0.001) and 100% of samples from G1 and G2, 59% from G3, and 36% from G4 reached the MIC for Streptococcus pneumoniae (p<0.001). For fluoroquinolone-resistant S. aureus, 23% from G1, 44% from G2, and no samples from G3 or G4 achieved the MIC (p<0.001). Conclusions: Moxifloxacin + dexamethasone demonstrated a higher concentration in the AH than the moxifloxacin alone. Gatifloxacin + steroids demonstrated less penetration into the anterior chamber than gatifloxacin alone. Moxifloxacin was superior to gatifloxacin considering the MIC for enterococci, S. pneumoniae, and fluoroquinolone-resistant S. aureus.
- ItemSomente MetadadadosAvaliação da produção intra-ocular de anticorpos anti-toxoplasma gondii no humor aquoso de pacientes com toxoplasmose ocular por ensaio imunoenzimático (ELISA) simplificado(Universidade Federal de São Paulo (UNIFESP), 1993) Petrilli, Ana Maria Noriega [UNIFESP]; Abreu, Mariza Toledo de [UNIFESP]
- ItemSomente MetadadadosBarreira hemato-aquosa no ceratocone(Universidade Federal de São Paulo (UNIFESP), 1984) Nosé, Walton [UNIFESP]; Belfort, Rubens Junior [UNIFESP]
- ItemSomente MetadadadosConcentração circadiana de peptídeo natriurético tipo C no humor aquoso de coelhos albinos e sua relação com a pressão intra-ocular e fluxo de aquoso(Universidade Federal de São Paulo (UNIFESP), 2001) Paranhos Junior, Augusto [UNIFESP]; Prata Junior, João Antonio [UNIFESP]
- ItemAcesso aberto (Open Access)Concentração de proteínas no humor aquoso de pacientes com glaucoma primário de ângulo aberto em tratamento clínico(Conselho Brasileiro de Oftalmologia, 2007-03-01) Prata, Tiago dos Santos [UNIFESP]; Navajas, Eduardo Vitor [UNIFESP]; Melo Junior, Luiz Alberto Soares [UNIFESP]; Martins, João Roberto Maciel [UNIFESP]; Nader, Helena Bonciani [UNIFESP]; Belfort, Rubens Junior [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To compare total protein concentration in the aqueous humor of primary open-angle glaucoma and non-glaucomatous patients. METHODS: Aqueous humor samples were obtained from 22 patients just before trabeculectomy for clinically uncontrolled primary open angle glaucoma (POAG group). Aqueous humor (0.1 mL) was aspirated by inserting a 26-gauge needle into the anterior chamber. The same procedure was performed in 22 non-glaucomatous patients just before cataract surgery (control group). Immediately after collection, the aqueous humor was stored at -20°C. Aqueous humor total protein concentration was determined using a colorimetric assay. RESULTS: The geometric mean of total protein concentration of the aqueous humor samples was 32 mg/dL (range: 8-137 mg/dL) in the primary open angle glaucoma group and 16 mg/dL (range: 2-85 mg/dL) in the control group. The ratio of the protein concentration between the two groups was 2.0 (95% confidence interval: 1.3 to 3.2; p=0.003). CONCLUSIONS: The total protein concentration in primary open-angle glaucoma aqueous humor was approximately two times higher than that in non-glaucomatous subjects.
- ItemAcesso aberto (Open Access)Efeito das técnicas osteopáticas sobre a pressão intraocular(Universidade Federal de São Paulo, 2023-04-03) Borges, Rodolfo Amoroso [UNIFESP]; Conte, Marcelo [UNIFESP]; http://lattes.cnpq.br/8945297747305462; http://lattes.cnpq.br/5362039462403519Objetivos: Verificar variação aguda da pressão intraocular (PIO) após a realização de técnicas osteopáticas. Métodos: Foram selecionados sete voluntários de ambos os gêneros entre 19 e 47 anos e com pressão intraocular entre 9 e 21mmHg. Todos os voluntários foram submetidos, aleatoriamente, a três intervenções: Intervenção Controle (IC), Intervenção Experimental 1 (IE1) e Intervenção Experimental 2 (IE2). No IC, o voluntário ficou deitado na maca por 5 minutos. No IE1, o voluntário recebeu 2 manipulações das vértebras da região da cervical alta (C1 e C2), uma para cada lado de rotação e no IE2, o voluntário recebeu uma manipulação indireta do gânglio esfenopalatino bilateralmente por via oral. A PIO foi medida, utilizando Tonômetro de Perkins, em ambos os olhos antes dos procedimentos e logo em seguida de cada procedimento. Resultados: Para a análise estatística, foi realizada a Análise de Variância (ANOVA) em blocos. Houve diferença estatística entre os tratamentos (pré x IE1 x IE2 x IC) para o olho direito (OD) (p<0,001) e olho esquerdo (OE) (p<0,001). Os valores médios da PIO pré-intervenção para o OD foi de 11,424±2,44 e para o OE, 11,571±2,225, a média da PIO pós IE1 para o OD foi de 9,714±2,289 e para o OE de 9,286±1,604, a média da PIO pós IE2 para o OD foi de 9,857±2,116 e para o OE de 9,857±2,268 enquanto que a média da PIO do IC para o OD foi de 11,286±2,563 e para o OE de 11±1,633. Conclusão: As técnicas osteopáticas se mostraram eficazes na diminuição aguda da pressão intraocular de ambos os olhos.
- ItemAcesso aberto (Open Access)Endoftalmites bacterianas com culturas positivas: uma revisão de 6 anos(Conselho Brasileiro de Oftalmologia, 2008-10-01) Bispo, Paulo José Martins [UNIFESP]; Melo, Gustavo Barreto de [UNIFESP]; D'azevedo, Pedro Alves; Hofling-Lima, Ana Luisa [UNIFESP]; Yu, Maria Cecília Zorat [UNIFESP]; Pignatari, Antonio Carlos Campos [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Fundação Faculdade Federal de Ciências Médicas de Porto Alegre - FFFCMPA Departamento de Microbiologia e ParasitologiaPURPOSE: To assess the distribution of microorganisms isolated from patients with bacterial endophthalmitis and their antimicrobial susceptibility. METHODS: Retrospective analysis of medical and microbiological records of patients with suspected diagnosis of endophthalmitis and bacterial culture-proven at the Department of Ophthalmology, UNIFESP, between January 1 2000 and December 31 2005. RESULTS: 153 (33.9%) of 451 patients showed positive bacterial culture. A total of 155 microorganisms were isolated, 79.35% were gram-positive and 20.65% gram-negative. Staphylococcus (CoNS) (41.94%) were the most frequently isolated. The antimicrobial susceptibility for gram-negative microorganisms was as follows: amikacin 87.10%, tobramycin 80.65%, ciprofloxacin 96.67%, levofloxacin, gatifloxacin and moxifloxacin 100%, ceftazidime 85.0%, and gentamicin 80.65%. Vancomycin sensitivity among gram-positive microorganisms was 100%. S. aureus and CoNS showed 83.33% of susceptibility to oxacillin, 89.61% to ciprofloxacin and 100% to gatifloxacin and moxifloxacin. The main acquisition mechanism was postoperative (60.65%). CONCLUSION: We detected a low sensitivity of vitreous/aqueous culture for the etiologic diagnosis of endophthalmitis. The empiric antimicrobial therapy or prophylaxis should be active against gram-positive bacteria, particularly staphylococci. Surveillance studies of bacterial resistance are important for a better utilization of antimicrobials in this clinical setting.
- ItemSomente MetadadadosEstudo das subpopulações de linfócitos TH e TS no humor aquoso e no sangue periférico de pacientes portadores de uveites ativas não tratadas(Universidade Federal de São Paulo (UNIFESP), 1984) Abreu, Mariza Toledo de [UNIFESP]; Belfort, Rubens Junior [UNIFESP]
- ItemSomente MetadadadosEstudo dos leucotrienos no humor aquoso e soro de pacientes portadores de uveites e controles(Universidade Federal de São Paulo (UNIFESP), 1989) Abreu, Mariza Toledo de [UNIFESP]; Belfort, Rubens Junior [UNIFESP]
- ItemAcesso aberto (Open Access)Lack of circadian change of concentration of C-type natriuretic peptide in rabbit aqueous humor(Conselho Brasileiro de Oftalmologia, 2006-10-01) Paranhos Junior, Augusto [UNIFESP]; Okada, Koji; Mello, Paulo Augusto de Arruda [UNIFESP]; Prata Junior, João Antonio [UNIFESP]; Gregory, Douglas; Universidade Federal de São Paulo (UNIFESP); Universidade de YALEPURPOSE: To determine whether there is a circadian change of the concentration of C-type natriuretic peptide (CNP) in rabbit aqueous humor. METHODS: Forty-one male white New Zealand rabbits were submitted to a 12 h light and 12 dark lighting schedule; lights on was at 0 h, lights off at 12 h. C-type natriuretic peptide was assayed at 3 light (2 h, 6 h and 10 h) and 3 dark (14,18 and 22 h) times. All groups consisted of four animals but two had more animals to increase the power of tests (6 h, n=12; 22 h, n=13). Dependence between the two eyes was tested by Pearson's correlation. The mean of two eyes was considered for analysis. Differences in concentration in pg/200 ml were evaluated by one-way ANOVA, t test and COSINOR analysis. RESULTS: There were no significant differences between the 6 analyzed groups (one-way ANOVA p=0.157). Pooled into two groups (light and dark), the C-type natriuretic peptide concentration of the light group was lower, 2.626±0.92 pg/200 ml compared with the dark group 3.02±1.16 pg/200 ml but did not reach a significant difference (t test; p=0.23). COSINOR analysis was not statistically significant (R%=7.72 p=0.209). CONCLUSION: These data demonstrate that the concentration of CNP in aqueous humor did not show a statistically significant circadian change in rabbits entrained to a 12 h light: 12 h dark lighting schedule.
- ItemAcesso aberto (Open Access)Real-time PCR in infectious uveitis as an alternative diagnosis(Conselho Brasileiro de Oftalmologia, 2011-08-01) Santos, Fabio Felipe dos [UNIFESP]; Commodaro, Alessandra Gonçalves [UNIFESP]; Souza, Andrea Vieira de; Pinho, João Renato Rebello; Sitnik, Roberta; Garcia, Claudio [UNIFESP]; Ribeiro, Ana Lúcia Pereira [UNIFESP]; Muccioli, Cristina [UNIFESP]; Lottenberg, Cláudio Luiz [UNIFESP]; Rizzo, Luiz Vicente; Belfort, Rubens Junior [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Hospital Israelita Albert EinsteinPURPOSE: Uveitis is a major visual impairment disease affecting parts or the entire uveal tract and occasionally the sclera, the cornea or the optic nerve. The disease is a major cause of ocular morbidity and blindness in immunocompetent and immunocompromised patients. In this work we analyzed the sensitivity and specificity of real-time PCR to detect the etiological agent from blood, plasma, vitreous and aqueous humor and compared with the diagnostic hypothesis. METHODS: Twenty-seven patients (13 male) were studied and Real-time PCR method was used for the detection of herpes simplex virus 1 (HSV-1), herpes simplex virus 2 (HSV-2), varicella zoster virus (VZV), cytomegalovirus (CMV), Mycobacterium tuberculosis (TB) and Toxoplasma gondii (Toxo) in the aqueous humor as well as in the vitreous, blood and plasma. RESULTS: Our results showed the presence of Toxo, CMV, VZV or HSV-2 in 19.2% of aqueous humor samples, and in 30% of vitreous humor samples. In plasma and blood samples, only CMV was detected (11.1% and 3.7%, respectively). CONCLUSION: Real-time PCR was able to detect and to confirm diagnostic hypothesis in uveitis. Our data also confirms that vitreous humor is the best source for molecular diagnosis of infectious uveitis but indicates aqueous humor samples that are easier to obtain may also be appropriate to be tested by Real-time PCR.