Repositório Institucional - Unifesp :: Navegando por Palavras-chave "Human"

Navegando por Palavras-chave "Human"

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Acesso aberto (Open Access)
Acute strength exercise and the involvement of small or large muscle mass on plasma brain-derived neurotrophic factor levels
(Faculdade de Medicina / USP, 2010-01-01) Correia, Paulo Roberto [UNIFESP]; Pansani, Aline Priscila [UNIFESP]; Machado, Felipe [UNIFESP]; Andrade, Marilia dos Santos [UNIFESP]; Silva, Antonio Carlos da [UNIFESP]; Scorza, Fulvio Alexandre [UNIFESP]; Cavalheiro, Esper Abrão [UNIFESP]; Arida, Ricardo Mario [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
OBJECTIVE: Blood neurotrophins, such as the brain-derived neurotrophic factor, are considered to be of great importance in mediating the benefits of physical exercise. In this study, the effect of acute strength exercise and the involvement of small versus large muscle mass on the levels of plasma brain-derived neurotrophic factor were evaluated in healthy individuals. METHODS: The concentric strengths of knee (large) and elbow (small) flexor and extensor muscles were measured on two separate days. Venous blood samples were obtained from 16 healthy subjects before and after exercise. RESULTS: The levels of brain-derived neurotrophic factor in the plasma did not significantly increase after both arm and leg exercise. There was no significant difference in the plasma levels of the brain-derived neurotrophic factor in the arms and legs. CONCLUSION: The present results demonstrate that acute strength exercise does not induce significant alterations in the levels of brain-derived neurotrophic factor plasma concentrations in healthy individuals. Considering that its levels may be affected by various factors, such as exercise, these findings suggest that the type of exercise program may be a decisive factor in altering peripheral brain-derived neurotrophic factor.
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Aspectos morfológicos das gônadas de mulheres com insuficiência ovariana prematura
(Universidade Federal de São Paulo (UNIFESP), 1991) Haidar, Mauro Abi [UNIFESP]; Baracat, Edmund Chada [UNIFESP]
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Best practice for PTEN gene and protein assessment in anatomic pathology
(Elsevier B.V., 2014-01-01) Carvalho, Katia C.; Maia, Beatriz M.; Omae, Samantha V.; Rocha, Antonio A. [UNIFESP]; Covizzi, Luiz P.; Vassallo, Jose; Rocha, Rafael M.; Soares, Fernando A.; Universidade de São Paulo (USP); Hosp AC Camargo Fund Antonio Prudente; Universidade Federal de São Paulo (UNIFESP)
There is a lack of standardization of a best practice protocol for Phosphatase and Tensin Homolog (PTEN) assessment by immunohistochemistry in anatomic pathology routine practice. We performed immunohistochemistry for 19 antibodies against PTEN, eleven of which were excluded during the standardization step. Immunohistochemistry of the remaining eight antibodies was performed on a Tissue Microarray containing 55 prostate and 40 renal carcinoma samples. Fluorescent in situ hybridization (FISH) was used as reference standard for immunohistochemistry specificity evaluation. Concerning nuclear staining, polyclonal (Cat#22034-1-AP); 6H2.1 mMAb (Cat#ABM-2052), Y184 RabMAb (Cat#NB110-57441) and 217702 mMAb antibodies presented the highest agreement with fluorescent in situ hybridization (p<0.001 for all) and with regard to cytoplasmic staining, Y184 RabMAb (Cat#NB110-57441); polyclonal (Cat#22034-1-AP) and 217702 mMAb presented the highest agreement (p < 0.001 for all). Our results indicate that several commercially available antibodies do not show reliability of sensitivity and specificity for PTEN evaluation and we propose 6H2.1 mMAb (Cat#ABM-2052) as the antibody of choice for laboratory standardization and best practice in clinical routine, which demonstrated excellent sensitivity for both nuclear and cytoplasmic staining, specificity for PTEN by Western blot and good correlation with PTEN status by FISH with regard to nuclear staining. (C) 2013 Elsevier GmbH. All rights reserved.
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Breast cancer tissue slices as a model for evaluation of response to rapamycin
(Springer, 2013-06-01) Giorgi Grosso, Stana Helena; Hirata Katayama, Maria Lucia; Roela, Rosimeire Aparecida; Nonogaki, Suely; Soares, Fernando Augusto; Brentani, Helena; Lima, Leandro; Azevedo Koike Folgueira, Maria Aparecida; Logullo Waitzberg, Angela Flavia [UNIFESP]; Pasini, Faima Solange; Guedes Sampaio Goes, Joao Carlos; Mitzi Brentani, M.; Inst Brasileiro Controle Canc; Universidade de São Paulo (USP); Adolfo Lutz Inst; Hosp A C Camargo; Hosp AC Camargo Fund Antonio Prudente; Universidade Federal de São Paulo (UNIFESP)
Rapamycin is a selective inhibitor of the mammalian target of rapamycin (mTOR), a regulator kinase that integrates growth factors signaling via the phosphoinositide-3-kinase pathway and that has emerged as a novel therapeutic modality in breast cancer (BC). We propose a pre-clinical ex-vivo personalized organotypic culture of BC that preserves the microenvironment to evaluate rapamycin-mediated gene expression changes. Freshly excised ductal invasive BC slices, 400 mu m thick (n=30), were cultured in the presence or absence (control) of rapamycin (20 nM) for 24 h. Some slices were formalin-fixed for immunohistochemical determinations and some were processed for microarray analysis. Control slices in culture retained their tissue morphology and tissue viability (detected by BrdU uptake). the percentage of proliferating cells (assessed by Ki67) did not change up to 24 h of treatment. Immunohistochemical evaluation of p-AKT, p-mTOR, p-4EBP1 and p-S6K1 indicated that AKT/mTOR pathway activation was maintained during cultivation. for microarray analysis, slices were divided into two groups, according to the presence/absence of epidermal growth factor receptor-type 2 and analyzed separately. Limited overlap was seen among differentially expressed genes after treatment (P < 0.01) in both groups suggesting different responses to rapamycin between these BC subtypes. Ontology analysis indicated that genes involved in biosynthetic processes were commonly reduced by rapamycin. Our network analysis suggested that concerted expression of these genes might distinguish controls from treated slices. Thus, breast carcinoma slices constitute a suitable physiological tool to evaluate the short-term effects of rapamycin on the gene profile of individual BC samples.
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Bronchial tree Architecture in Mammals of Diverse Body Mass
(Soc Chilena Anatomia, 2014-03-01) Monteiro, Adilson [UNIFESP]; Smith, Ricardo Luiz [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
The anatomy of mammal's lung air space constitutes the bronchial tree which disposition is associated to air flux dynamics. Casts obtained from human, pig and rat lungs were studied to analyze possible differences of the bronchial tree architecture in mammals with diverse dimensions and posture. Air spaces were filled with polymers through trachea followed by acid corrosion. Tracheal and main bronchial division's diameters were measured to relate with body mass using allometry. The results revealed a dichotomic bronchial branching pattern in the human casts and a monopodial pattern in animals. In allometric relationship trachea was larger in rats, then pigs and lastly in humans, differences were statistically significant, the same occurs in right bronchus, as in the left bronchus there was no difference between rat and pig. The linear relationship between the human tracheal diameters was 1.2 times larger than the pig and 6.7 times larger than the rat; the pig tracheal diameter was 5.6 times larger than the rat. Quadruped position of the pig and rat is linked to a horizontal air way while the erect position, biped in human, correspond to a vertical air way. A big mammal shows less respiratory frequency than small mammals. Mammals with small, medium and high body mass allied to diverse posture and habits was compared revealing morphological differences in the bronchial trees as different allometric correlations between quadruped animals and human biped.
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Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract
(Oxford Univ Press, 2008-02-01) Patrão, Marilia Tavares Coutinho da Costa [UNIFESP]; Queiroz, Daniel Barboza Cava [UNIFESP]; Grossman, Gail; Petrusz, Peter; Lazari, Maria de Fatima Magalhaes [UNIFESP]; Avellar, Maria Christina Werneck [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ N Carolina
This study reports the genomic organization of the rhesus alpha(1A)-adrenoceptor gene (ADRA1A). Full-length cloning of rhesus ADRA1A splice variants was achieved by combining PCR screening of a seminal vesicle cDNA library and 5'-RACE assays with total RNA from seminal vesicle. the classical ADRA1A mRNA (ADRA1A_nu 1) and six full-length ADRA1A splice variants were identified representing transcripts that code for functional (ADRA1A_nu 1, ADRA1A_nu 2a, ADRA1A_nu 3a, ADRA1A_nu 3d, ADRA1A_nu 3e) and truncated (ADRA1A_nu 2c and ADRA1A_nu 3c) receptor isoforms. Comparative analysis of the deduced amino acid sequence indicated that rhesus ADRA1A_i1 isoform (corresponding to the ADRA1A_nu 1 transcript) shares high identity to the amino acid sequence present in the classical alpha(1A)-adrenoceptor from human and other mammalian species. Partial nucleotide sequences for rhesus alpha(1B)-(ADRA1B) and alpha(1D)-adrenoceptor (ADRA1D) transcripts were also characterized. RT-PCR studies indicated differential distribution of all ADRA1A-related splice variants as well as ADRA1B and ADRA1D mRNAs, in tissues from rhesus and human male reproductive tract. Immunohistochemistry revealed alpha(1A)-adrenoceptor (ADRA1A_ i1) immunostaining in smooth muscle cells and epithelial cells of rhesus efferent ductules, epididymis and seminal vesicle. Taken together the present results demonstrate that the complexity of the splicing mechanisms involved in the regulation of the ADRA1A gene is not restricted to human and is a common characteristic among Old World monkeys.
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Considerações anatômicas sobre a artéria torácica interna: Seus ramos esternais e sua importância na cirurgia torácica
(Universidade Federal de São Paulo (UNIFESP), 2010-04-28) Itezerote, Ana Maria [UNIFESP]; Smith, Ricardo Luiz [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Objective: The internal thoracic artery is responsible for irrigation of the sternum. The sternum consists of three parts, the manubrium, body of sternum and xiphoid process. The aim of this study was to analyze irrigation, by the branches of internal thoracic artery, towards the posterior side of the manubrium, in which we can observe the insertions of sternothyroid and sternohyoid muscles as well as the branches of internal thoracic artery to the body and xiphoid process. Methods: Sixty male and female the anterior sternocostal wall of the thorax previously fixed in formaldehyde solution, with ages ranging from 20 to 80 years were used from the Service of Verification of Death (SVO) of the Federal University of São Paulo - Paulista School of Medicine. The research project was approved by the Ethics Committee of the Federal University of São Paulo under number: 0109/06 on 02/01/2006. In these the anterior sternocostal wall of the thorax. the right and left internal thoracic arteries had already been partially dissected by the graduate students, and injected with colored neoprene latex, yellow in left internal thoracic arteries and red in right side arteries. It was used a dissecting microscope with 10X, dissecting forceps and sharp and blunt point scissors. The trunk and branches of internal thoracic arteries were dissected and after being measured they were recorded in a specific file, the material was photographed at different angles and close. In statistical analysis the paired t test, analysis of variance (ANOVA) and Tukey technique were employed. Results: The sternothyroid and sternohyoid muscles, with their insertions in the manubrium were irrigated by sternal branches in all 120 studied internal thoracic arteries. The body of the sternum received sternal branches originated from both internal thoracic artery and the branches arising directly from it. A difference was found regarding the amount of trunks and sternal branches in different sternal intercostal spaces of 60 the anterior sternocostal wall of the thorax. The xiphoid process was irrigated from the sternal branches of the internal thoracic artery and the superior epigastric artery, branches only of the superior epigastric and irrigation of their own superior epigastric artery. Conclusions: in the final considerations it was reported that the internal thoracic artery actively participates in the irrigation, insertions of the sternothyroid and sternohyoid muscles and, therefore, manubrium. The internal thoracic artery is the third arterial pedicle for the sternothyroid and sternohyoid muscles. The sternal branches are in greater quantities than those originating from branches of the sternal trunks. The preservation of these trunks is needed to keep the blood supply of the sternum, when suffering sternotomy and using both internal thoracic arteries. The xiphoid process provides irrigation from different vessels, but its maintenance through the superior epigastric artery is relevant since this vessel is richly distributed on the posterior and anterior xiphoid cartilage.
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Contribuição ao estudo da insuficiência istmocervical e seu tratamento pela circlagem, segundo técnica proposta por McDonald
(Universidade Federal de São Paulo (UNIFESP), 1986) Mattar, Rosiane [UNIFESP]; Camano, Luiz [UNIFESP]
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Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
(Journal Of Visualized Experiments, 2017) Cabral, Thiago [UNIFESP]; Toral, Marcus A.; Velez, Gabriel; DiCarlo, James E.; Gore, Anuradha M.; Mahajan, MaryAnn; Tsang, Stephen H.; Bassuk, Alexander G.; Mahajan, Vinit B.
The human retina is composed of the sensory neuroretina and the underlying retinal pigmented epithelium (RPE), which is firmly complexed to the vascular choroid layer. Different regions of the retina are anatomically and molecularly distinct, facilitating unique functions and demonstrating differential susceptibility to disease. Proteomic analysis of each of these regions and layers can provide vital insights into the molecular process of many diseases, including Age-Related Macular Degeneration (AMD), diabetes mellitus, and glaucoma. However, separation of retinal regions and layers is essential before quantitative proteomic analysis can be accomplished. Here, we describe a method for dissection and collection of the foveal, macular, and peripheral retinal regions and underlying RPE-choroid complex, involving regional punch biopsies and manual removal of tissue layers from a human eye. One-dimensional SDS-PAGE as well as downstream proteomic analysis, such as liquid chromatography-tandem mass spectrometry (LC-MS/MS), can be used to identify proteins in each dissected retinal layer, revealing molecular biomarkers for retinal disease.
Acesso aberto (Open Access)
Does the change on gastrointestinal tract microbiome affects host?
(Brazilian Society of Infectious Diseases, 2014-12-01) Beirão, Elisa M.; Padovan, Ana Carolina Barbosa [UNIFESP]; Furtado, Juvêncio J.d.; Colombo, Arnaldo Lopes [UNIFESP]; Medeiros, Eduardo Alexandrino Servolo de [UNIFESP]; Hospital Heliópolis Department of Infectiology; Universidade Federal de São Paulo (UNIFESP)
During the past decade, studies on the composition of human microbiota and its relation to the host became one of the most explored subjects of the medical literature. The development of high-throughput molecular technologies allowed a deeper characterization of human microbiota and a better understanding of its relationship with health and disease. Changes in human habits including wide use of antimicrobials can result in dysregulation of host–microbiome homeostasis, with multiple consequences. The purpose of this review is to highlight the most important evidence in the literature of host–microbiome interactions and illustrate how these intriguing relations may lead to new treatment and prevention strategies.
Acesso aberto (Open Access)
Efeito antitumoral do ácido graxo docosahexaenóico em células de glioblastoma humano
(Universidade Federal de São Paulo (UNIFESP), 2017-02-24) Queiroz, Fernanda de Oliveira [UNIFESP]; Miranda Filho, Manoel de Arcisio [UNIFESP]; http://lattes.cnpq.br/2680038286691388; http://lattes.cnpq.br/4963386651580275; Universidade Federal de São Paulo (UNIFESP)
Glioblastoma Multiforme is considered one of the most serious and common brain neoplasias, representing 50% of ali malignant tumors present in the brain. With a poor prognosis, half of the patients die within 1 year of diagnosis. Gliomas present great changes in their morphology, so they are considered tumors of high complexity. These morphological changes are one of the causes for resistance to existing therapies. In order to look for new alternatives for the treatment of these neoplasias, several compounds have been studied, among them, the fatty acids. Fatty acids are organic components, poorly soluble in water and play important roles in cell membranes and metabolic processes. They are divided into saturated and unsaturated. In order to understand the action of saturated fatty acids, palmitic acid and polyunsaturated fatty acids, docosahexaenoic acid (DHA) and arachidonic acid (AA) in head cancer, in this study we used glycoproteinoma multiforme cells from the T98G lineage. Different assays were performed to elucidate the mechanism of operation. Initially, cell viability tests were performed by MTT to observe whether these compounds cause death or the reduction of cell viability. In sequence, membrane fluidity tests were performed to understand the selectivity of DHA, since only this acid had a reduction in the viability of human glioblastoma cells. To understand and quantify the type of death, whether apoptotic or necrotic, flow cytometry and Co-incubation Hoechst 33342 / PI were performed at different concentrations and observed a higher AnexinN labeling in these cells. Nuclear morphological changes in T98G cells were observed from Hoechst .33342 cells. Data obtained showed fragmented, picnotic and condensed nuclei, which are characteristics of cellular apoptosis. Levels of reactive oxygen species were also performed. With the use of the DHE probe, we observed an increase in the production of reactive oxygen species (ROS), which indicates that the type of death we are observing is apoptosis. The viability of DHA is probably due to the induction of the production of high levels of ROS.
Acesso aberto (Open Access)
Efeito da riboflavina tópica exposta à irradiação ultravioleta A e inserção de segmentos de anéis corneanos intraestromais para ceratocone
(Universidade Federal de São Paulo (UNIFESP), 2012-05-30) Renesto, Adimara da Candelaria [UNIFESP]; Campos, Mauro Silveira de Queiroz [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Purpose: To report the impression cytologic results after corneal cross-linking and insertion of intrastromal corneal ring segments for keratoconus. Methods: Thirty-nine eyes were distributed into two groups: 1) cross-linking group (patients underwent corneal cross-linking procedure), and 2) riboflavin eyedrops group (patients received riboflavin 0.1% (w/v) eyedrops in 20% dextran solution for 1 month). After 3 months, all patients underwent insertion of intrastromal corneal ring segments. Impression cytologic specimens were obtained from all eyes at baseline, at 1 month and 3 months after cross-linking or riboflavin eyedrops, and again at 6 months, 1 year, and 2 years after intrastromal corneal ring segment insertion. Results: Patients in the cross-linking group demonstrated improvement in the cell-to-cell contact of epithelial cells and the nucleusto- cytoplasm ratio on the temporal conjunctiva after treatment (P = 0.008 and P = 0.047), respectively. On the superior conjunctiva, increases in goblet cell density (P = 0.037) and level of organization of nuclear chromatin (P = 0.010) after treatment were noted. Patients in the riboflavin eyedrops group demonstrated improvement in the cellto- cell contact of epithelial cells on the superior conjunctiva after treatment (P = 0.021). On the temporal conjunctiva, an improvement in the cell-to-cell contact of epithelial cells (P < 0.001) and increases in the nucleus-to-cytoplasm ratio (P < 0.001), goblet cell density (P = 0.001), and less keratinization (P = 0.011) were noted. No changes were identified on the cornea for either group. Fisher’s exact test comparison of the impression cytologic total scores after treatment revealed no difference between groups. Conclusion: Despite changes in some conjunctival parameters (e.g., cell-tocell contact of epithelial cells, nucleus-to-cytoplasm ratio, level of organization of nuclear chromatin , goblet cell density, and keratinization), comparison of the total impression cytologic scores revealed no difference between groups.
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Immune alterations after selective rapid eye movement or total sleep deprivation in healthy male volunteers
(Sage Publications Ltd, 2012-02-01) Ruiz, Francieli S.; Andersen, Monica L. [UNIFESP]; Martins, Raquel C. S.; Zager, Adriano; Lopes, Jose D.; Tufik, Sergio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
We investigated the impact of two nights of total sleep deprivation (SD) or four nights of rapid eye movement (REM) SD on immunological parameters in healthy men. Thirty-two volunteers were randomly assigned to three protocols (control, total SD or REM SD). Both SD protocols were followed by three nights of sleep recovery. the control and REM SD groups had regular nights of sleep monitored by polysomnography. Circulating white blood cells (WBCs), T-(CD4/ CD8) and B-lymphocytes, Ig classes, complement and cytokine levels were assessed daily. Two nights of total SD increased the numbers of leukocytes and neutrophils compared with baseline levels, and these levels returned to baseline after 24 h of sleep recovery. the CD4(+) T-cells increased during the total SD period (one and two nights) and IgA levels decreased during the entire period of REM SD. These levels did not return to baseline after three nights of sleep recovery. Levels of monocytes, eosinophils, basophils and cytokines (IL-1 beta, IL-2, IL-4, IL-6, IL-10, TNF-alpha and IFN-gamma) remained unchanged by both protocols of SD. Our findings suggest that both protocols affected the human immune profile, although in different parameters, and that CD4(+) T-cells and IgA levels were not re-established after sleep recovery.
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Increased interferon-mediated immunity following in vitro and in vivo Modafinil treatment on peripheral immune cells
(Pergamon-Elsevier Science Ltd, 2018) Zager, Adriano; Brandao, Wesley Nogueira; Margatho, Rafael Oliveira; Gimenes Cruz, Daniel Sanzio; Peron, Jean Pierre; Tufik, Sergio [UNIFESP]; Andersen, Monica Levy [UNIFESP]; Moresco, Monica; Pizza, Fabio; Plazzi, Giuseppe; Kornum, Birgitte Rahbek; Palermo-Neto, Joao
The wake-promoting drug Modafinil has been used for treatment of sleep disorders, such as Narcolepsy, excessive daytime sleepiness and sleep apnea, due to its stimulant action. Despite the known effect of Modafinil on brain neurochemistry, particularly on brain dopamine system, recent evidence support an immunomodulatory role for Modafinil treatment in neuroinflammatory models. Here, we aimed to study the effects of in vitro and in vivo Modafinil treatment on activation, proliferation, cell viability, and cytokine production by immune cells in splenocytes culture from mice. The results show that in vitro treatment with Modafinil increased Interferon (IFN)-gamma, Interleukin (IL)-2 and IL-17 production and CD25 expression by T cells. In turn, in vivo Modafinil treatment enhanced splenocyte production of IFN-gamma, IL-6 and tumor necrosis factor (TNF), and increased the number of IFN-gamma producing cells. Next, we addressed the translational value of the observed effects by testing PBMCs from Narcolepsy type 1 patients that underwent Modafinil treatment. We reported increased number of IFN-gamma producing cells in PBMCs from Narcolepsy type 1 patients following continuous Modafinil treatment, corroborating our animal data. Taken together, our results show, for the first time, a pro-inflammatory action of Modafinil, particularly on IFN-mediated immunity, in mice and in patients with Narcolepsy type 1. The study suggests a novel effect of this drug treatment, which should be taken into consideration when given concomitantly with an ongoing inflammatory or autoimmune process.
Acesso aberto (Open Access)
Infectious keratitis in patients undergoing Boston Type 1 keratoprosthesis (Boston KPro) procedure: case series
(Conselho Brasileiro de Oftalmologia, 2011-04-01) Nascimento, Heloisa Moraes do [UNIFESP]; Oliveira, Lauro Augusto de [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Description of two cases of infectious keratitis in patients after Boston Type 1 keratoprosthesis (Boston KPro) implantation. The first case refers to a patient that had the device indicated due to limbal deficiency secondary to severe dry eye who presented a fungal infection by Aerobasidium pullulans that was successfully treated with amphotericin B eye drops. The second case reports a patient with Boston KPro implantation due to previous corneal transplant rejection showing bacterial keratitis in the fourth postoperative month. The etiologic agent was identified asStreptococcus sp and topical treatment with vancomycin was effective. The importance of postoperative surveillance in Boston KPro eyes is discussed.
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Integrin-Linked Kinase (ILK) Expression Correlates with Tumor Severity in Clear Cell Renal Carcinoma
(Springer, 2013-01-01) Brasil Engelman, Miriam de Fatima; Grande, Rogerio Mendes; Naves, Marcelo Andery [UNIFESP]; Franco, Marcello Fabiano de [UNIFESP]; Teixeira, Vicente de Paulo Castro [UNIFESP]; Univ Vale Sapucai; Universidade Federal de São Paulo (UNIFESP)
Integrin-linked kinase (ILK) is an unique intracellular serine/threonine kinase and adapter protein. When dysregulated, it has been associated with increased cell proliferation, anchorage-independent cell growth, evasion of apoptosis, angiogenesis, invasion of surrounding tissues, downregulation of E-cadherin expression, nuclear translocation of beta-catenin and metastasis, all features of tumoral malignancy. the objective of the present work was to evaluate the expression of ILK in clear cell renal carcinomas (CCRC) as a possible prognostic indicator. ILK immunoexpression was evaluated in a tissue microarray (TMA) with 45 human CCRCs. in addition, the apoptotic and proliferative indices and the immuno-expression of beta-catenin and E-cadherin were also evaluated. E-cadherin expression was significantly decreased in tumors with positive ILK expression in relation to those with negative immunoexpression (p = 0.011). ILK immunostaining was significantly increased in high-grade in comparison to low-grade CCRCs (p = 0.0008). ILK expression was also associated with increased proliferative index (p = 0.020), tumor size > 7.0 cm (p = 0.018) and with renal vein and capsule invasion (p = 0.003 and p = 0.00). Finally, tumors stage I and II (noninvasive) presented significantly reduced ILK immunoexpression when compared to stage III (locally invasive) (p = 0.0028). ILK immunoexpression in CCRC increases with loss of intercellular adhesion, nuclear grading, increased proliferative index and Robson stage. Altogether, our data suggest a possible role for ILK in the progression of CRCC.
Acesso aberto (Open Access)
Intraretinal worm documented by optical coherence tomography in a patient with diffuse unilateral subacute neuroretinitis: case report
(Conselho Brasileiro de Oftalmologia, 2010-10-01) Cunha, Leonardo Provetti; Costa-cunha, Luciana Virgínia Ferreira; Souza, Eduardo Cunha De [UNIFESP]; Monteiro, Mário Luiz R.; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
Our purpose is to report a case of diffuse unilateral subacute neuroretinitis (DUSN) in which an ophthalmoscopically visible worm was found and optical coherence tomography (OCT) scans allowed the precise localization of the parasite in the intraretinal layers. Our findings suggest that the parasite moves in the inner portions of the retina possibly explaining the severe degenerative neural changes that it causes.
Acesso aberto (Open Access)
Lipemia retinalis in a 35-day-old infant with hyperlipoproteinemia: case report
(Consel Brasil Oftalmologia, 2008-03-01) Cypel, Marcela [UNIFESP]; Manzano, Roberta [UNIFESP]; Reis, Frederico Augusto dos [UNIFESP]; Ishida, Noemi; Ayhara, Teruo; Universidade Federal de São Paulo (UNIFESP); Santa Casa Misericordia São Paulo
A rare case of hyperlipropoteinemia in a 35-day-old infant who presented not only high blood levels of cholesterol and triglycerides but also an ocular manifestation described as lipemia retinalis. the fundoscopic abnormality cleared as the levels of chilomicrons in plasma dropped. Lipemia retinalis is an important and reliable parameter of high levels of chilomicrons and triglycerides and should be considered as a significant clue while diagnosing.
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Long-term nutrition education reduces several risk factors for type 2 diabetes mellitus in Brazilians with impaired glucose tolerance
(Elsevier B.V., 2010-03-01) Pimentel, Gustavo D. [UNIFESP]; Portero-Mclellan, Katia C.; Oliveira, Erick P.; Spada, Ana P. M.; Oshiiwa, Marie; Zemdegs, Juliane C. S. [UNIFESP]; Barbalho, Sandra M.; Universidade Federal de São Paulo (UNIFESP); Pontificia Univ Catolica Campinas; Universidade de São Paulo (USP); Univ Marilia
The aim of this study was to evaluate the effects of a nutrition education program (NEP) on anthropometric, dietetic, and metabolic parameters in high-risk subjects for type 2 diabetes mellitus. Fifty-one participants, both sexes, were randomly assigned to either the control (58.8%) or the intervention (NEP) group. the intervention group received frequent individual and group nutritional counseling from a team of nutritionists. Participants were assessed at baseline (M0) and after 12 months (M1) for anthropometric, dietetic, and metabolic parameters. the hypothesis was that high-risk subjects for type 2 diabetes mellitus participating in NEP would show an improvement in these parameters. At M1, the intervention group showed a significant decline in body weight (-3.4%), body mass index (-5.7%), cholesterol intake (-49.5%), fasting glycemia (-14.0%), fasting insulin (-9.0%), postprandial glycemia (-21.0%), postprandial insulin (-71.0%), total serum cholesterol (-23.0%), and glycated hemoglobin (-24.0%). A decrease in energy intake (5%, P = .06) and low-density lipoprotein cholesterol (25%, P = .07) was observed in the interventional group, although it did not reach statistical significance. in contrast, the control group presented a significantly higher energy intake (19%, P = .04) and a nonsignificant increase in consumption of all macronutrients. the long-term NEP was found to improve anthropometric, dietary, and metabolic parameters in high-risk subjects for type 2 diabetes mellitus. (C) 2010 Elsevier Inc. All rights reserved.
Acesso aberto (Open Access)
Management of traumatic macular holes: case report
(Conselho Brasileiro de Oftalmologia, 2008-08-01) Brasil, Oswaldo Ferreira Moura [UNIFESP]; Brasil, Oswaldo Moura [UNIFESP]; Instituto Brasileiro de Oftalmologia; Universidade Federal de São Paulo (UNIFESP)
Traumatic macular hole is a disease whose pathogenesis is not fully understood and the best treatment guideline is controversial. We report 2 cases of traumatic macular hole with different treatment approaches. In the first case, a 9-year-old boy presented with a traumatic macular hole secondary to blunt ocular trauma with a stone, and initial vision of 20/300. He underwent surgical repair and his final vision was 20/70 with hole closure after a 1 year follow-up. In the second case, a 20-year-old woman suffered a penetrating bullet wound on the left side of her forehead. The injury caused optic nerve head avulsion in the left eye with loss of light perception. The right eye had a traumatic macular hole and signs suggestive of sclopetaria chorioretinitis, with 20/60 vision. This case was initially observed and vision improved to 20/30 with reduction of the hole diameter. Vision and hole diameter remained stable after 8 months.

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