Navegando por Palavras-chave "Histochemistry"
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- ItemAcesso aberto (Open Access)Collagen and reticular fibers in left ventricular muscle in diabetic rats: Physical exercise prevents its changes?(Churchill Livingstone, 2011-02-01) Castellar, Alexandre; Remedio, Rafael Neodini; Barbosa, Rodrigo Avelaira; Gomes, Ricardo José [UNIFESP]; Caetano, Flavio Henrique; São Paulo State Univ; Universidade Federal de São Paulo (UNIFESP)Diabetic cardiomyopathy contributes to the high incidence of mortality in both types of diabetes. We aimed to investigate the histochemical aspects of collagen and reticular fibers in the cardiac muscle and evaluate the influence of physical exercise on these aspects. Wistar rats were divided in 4 groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Diabetes was induced with alloxan (35 mg/kg). Training program consisted of swimming 1 h/day with a load of 4.8% of body weight for TD and 5.2% for TC. during 8 weeks. At the end of the experiment, samples of the left ventricle were collected, fixed in Bouin and processed on historesin. Sections were stained with periodic acid of Schiff, picrosirius-hematoxylin and ammoniacal silver. the PAS technique shows that individuals of group SD presented more intense reaction that the other groups. Picrosirius-hematoxylin technique showed a possible deposition of collagen fibers in SD. the TD group presented a reaction a lot similar to the controls' for both techniques', showing a possible prevention of this deposition. These results indicate that physical exercises might have an important role on the prevention of some negative alterations caused by experimental diabetes. (C) 2010 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosHistochemical and ultrastructural analysis of hepatic glycogen and collagen fibers in alloxan-induced diabetic rats submitted to long-term physical training(Churchill Livingstone, 2011-08-01) Remedio, Rafael Neodini; Barbosa, Rodrigo Avelaira; Castellar, Alexandre; Gomes, Ricardo José [UNIFESP]; Caetano, Flavio Henrique; São Paulo State Univ UNESP; Universidade Federal de São Paulo (UNIFESP)Alterations in liver functions are common among diabetic patients, and many symptoms in the liver have been reported, including changes in glycogen stores and in the amount of collagen fibers. the practice of physical training and its morphological effects in this organ, however, are scarcely studied. in order to observe the morphological effects of alloxan-induced diabetes and the alterations arising from the practice of long-term chronic physical training in the liver, samples were collected and processed, and then analyzed by means of the histochemical techniques Periodic Acid-Schiff and Picrosirius-Hematoxylin, and ultrastructural cytochemical test of Afzelius. Through evaluation of the tissue, it was observed a drastic reduction in hepatic glycogen stores of sedentary diabetics, recovered in trained diabetic rats. Furthermore, it was detected a decrease in the content of perisinusoidal collagen fibers in the diabetic liver, also recovered due to the development of a training protocol. On ultrastructural level, cytochemical analysis confirmed the loss of glycogen and the recovery obtained by training. in conclusion, the practice of a long-term chronic physical training protocol may be considered an important assistant in the treatment of diabetes, mitigating the occurrence of possible damages to liver tissue. (C) 2011 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosHyaluronan involvement in the changes of mouse interpubic tissue during late pregnancy and post-partum(Elsevier B.V., 2008-08-01) Garcia, Eduardo A.; Veridiano, Adriano M.; Martins, Joao R. M. [UNIFESP]; Nader, Helena B. [UNIFESP]; Pinheiro, M. C. [UNIFESP]; Joazeiro, Paulo P.; Toledo, Olga M. S. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Campinas (UNICAMP)The present work quantifies hyaluronan (HA) during the late pregnancy and post-partum in order to provide a better understanding of the role of HA in the adaptations that occur in the pubic symphysis during this period. HA was quantified in situ (histochemically) and in interpubic tissue extracts by fluorimetric assay. Samples were taken from virgin mice and from pregnant animals at various stages of pregnancy: 12th- 18th days into pregnancy, the day of delivery (D19) and the 3rd and 5th day post-partum. the quantitative fluorimetric analysis indicated a gradual increase of HA in the interpubic tissue throughout late pregnancy (2.4-4.6 mu g/mg dry weight). This was followed by a decrease beginning on D19 (12.4 mu g/mg), reaching close to virgin levels (2.2 mu g/mg) on the 5th day post-partum. the same optical density changes could be seen in the HA staining. Furthermore, the histochemical analysis demonstrated the presence of HA both in the extracellular matrix of the tissue and within its cells. Such results indicate that the extracellular presence of HA may contribute to the transformation of the symphysis into a flexible structure. in addition, HA's intracellular presence (until the 18th day of pregnancy) may contribute to cellular proliferation. Finally, during parturition and on the 5th day post-partum, HA may contribute to the maintenance of the myofibroblastic phenotype of ligament cells, aiding the ligament involution after parturition. (c) 2008 International Federation for Cell Biology. Published by Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosSpecial dyeing, histochemistry, immunohistochemistry and ultrastructure: A study of mast cells/eosinophilic granules cells (MCs/EGC) from Centropomus parallelus intestine(Academic Press Ltd- Elsevier Science Ltd, 2017) Silva, Wemeson Ferreira da [UNIFESP]; Simões, Manuel de Jesus [UNIFESP]; Gutierre, Robson Campos [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Santos, Antenor Aguiar [UNIFESP]; Antoniazzi, Marta M.; Sasso, Gisela Rodrigues da Silva [UNIFESP]; Ranzani-Paiva, Maria Jose T.Intestine mast cells/eosinophilic granule cells (MCs/EGC) of the marine species Centropomus parallelus (fat snook) were first studied using light and electron microscopy techniques. Mast cells are cells from the connective tissue found in almost all organs and tissues of vertebrates. In fish, they appear in greater numbers in parts of their bodies that are exposed to their environment, such as skin, gills and intestine. The granules in fat snook's mast cell contain a variety of substances, such as histamine, heparin, chondroitin sulfate, serotonin, proteases and cytokines. The present study of intestine MCs/EGC was carried out in 20 specimens of fat snook. Samples of tissue were fixed in Bouin solution and in buffered formalin. Ferric hematoxylin - Congo red, pH6 acridine orange, pH2.5 and pH0,5 Alcian Blue (AB), toluidine blue, PAS, AB + PAS and immunohistochemistry protocols were used. In the mucosa and submucosa layers, MCs/EGCs granules with basic contents were evidenced by Congo red staining, and with acid contents granules were identified through pH 2.5 and 0,5 AB, and acridine orange. Basic and acid contents were simultaneously evidenced using ferric hematoxylin - Congo red stain. Metachromasia was observed in both mucosal and submucosal mast cells. Neutral glycoproteins were evidenced by using PAS protocol, glycosaminoglycan through AB and both simultaneously through AB + PAS. In immunohistochemistry assays, MCs/EGC were positive for tryptase, chymase and serotonin. As in mammals, the study of samples fixed in modified Karnovsky for transmission electron microscopy evidenced that most of the MCs granules were spherical and showed varying electron density, as described in previous reports on other teleost fish species. The metachromasia observed and the identification of tryptase, chymase and serotonin suggest a great similarity between fat snook's MCs/EGC and those described in the mucosa of mammals. (C) 2016 Elsevier Ltd. All rights reserved.
- ItemSomente MetadadadosUltrastructural alterations in colon absorptive cells of alloxan-induced diabetic rats submitted to long-term physical training(Wiley-Blackwell, 2012-10-01) Remedio, Rafael Neodini; Barbosa, Rodrigo Avelaira; Castellar, Alexandre; Gomes, Ricardo José [UNIFESP]; Caetano, Flavio Henrique; São Paulo State Univ UNESP; Universidade Federal de São Paulo (UNIFESP)Absorptive cells have notable importance for proper function of the colon, absorbing water and nutrients. in type I diabetes, hyperglycemia leads to remarkable alterations in cell structure. in absorptive cells, such changes may impair the function of the organ as a whole. Also, the effects of physical training, which plays crucial role in the treatment of diabetes, are not yet known in these cells. for this reason, to analyze the changes in colon epithelial absorptive cells of diabetic rats and the effects of physical training, Wistar rats were divided into four groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD), and trained diabetic (TD). the training protocol consisted of swimming for 60 min a day, 5 days per week, during 8 weeks. Colon samples were collected, processed, and evaluated by histochemical and ultrastructural techniques. Although histochemical analysis did not reveal major differences, significant morphological differences were ultrastructurally observed among groups, especially related to the structure of tight junctions, interdigitations, and microvilli, which became longer in diabetics, and whose length was reduced after physical training, as proved by statistical analysis. There were no relevant changes in organelles. Thus, the development of type I diabetes can lead to changes at ultrastructural level that, even subtle, may cause important alterations in cell function. the practice of physical training, in turn, proved to be an important ally in the treatment of such changes. However, it cannot be used singly for treating this disease, requiring the combined practice of other methods. Microsc. Res. Tech. 2012. (c) 2012 Wiley Periodicals, Inc.