Navegando por Palavras-chave "Histamine"
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- ItemSomente MetadadadosAcoustic rhinometry in nasal provocation tests in children and adolescents(Hindawi Ltd, 2016) Wandalsen, G. F. [UNIFESP]; Mendes, A. L. [UNIFESP]; Matsumoto, F. [UNIFESP]; Sole, D. [UNIFESP]Objectives: To standardize acoustic rhinometry (AR) in nasal provocation tests (NPTs) with histamine in children and adolescents. Patients and Methods: We performed a cross-sectional validation to compare AR with anterior active rhinomanometry (AAR) during histamine NPT in 20 children and adolescents with persistent allergic rhinitis and 20 controls. Changes in total nasal resistance (AAR) were compared with changes in nasal volume in the first 5 cm (V5). Results: Compared with controls, patients with rhinitis had significantly higher mean total nasal resistance (0.34 Pa/cm(3)/s vs 0.21 Pa/cm(3)/s
- ItemSomente MetadadadosA comparison of histamine effects on the sympathetic neurotransmission of testicular capsule and rat vas deferens(Springer, 2014-08-01) Silva Junior, Edilson Dantas da [UNIFESP]; Dantas Rodrigues, Juliano Quintella [UNIFESP]; Souza, Bruno Palmieri de [UNIFESP]; Caricati-Neto, Afonso [UNIFESP]; Jurkiewicz, Aron [UNIFESP]; Jurkiewicz, Neide H. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Histamine is an important modulatory agent of the sympathetic neurotransmission, but its exact action on the testicular capsule or rat vas deferens is not fully understood. the present study sought to further investigate the functional effects of histamine on the neuronal and exogenous noradrenaline-induced contraction of the testicular capsule and rat vas deferens as well as to evaluate the contractile properties of this drug. the testicular capsule or vas deferens from Wistar rats, 3-4 months old, weighing 300-400 g, was isolated and mounted in organ baths for functional experiments. the results indicated that the neuronally evoked contraction of the testicular capsule was affected by histamine (10(-10) to 10(-8) M) with participation of inhibitory (H-3 receptors) and excitatory (H-1 receptors) receptors. Histamine (10(-7) to 10(-4) M) modulated the field-stimulated vas deferens by excitatory (H-2 receptors) and inhibitory (H-1 receptors) receptors. Histamine was able to decrease the tonic response for noradrenaline-induced contractions with participation of H-1 receptors (testicular capsule) and H-3 receptors (vas deferens) followed by nitric oxide generation. At high concentration, histamine exerts contractile effects in both tissues. in the testicular capsule, the histamine-induced contractions were related to H-1 receptor activation followed by release of prostaglandins. in contrast, the contractile effects of histamine in the vas deferens were related to H-2 receptor activation followed by release of catecholamines from sympathetic nerve endings. Therefore, our results indicate that histamine induced several effects on the sympathetic neurotransmission of rat testicular capsule and vas deferens. These effects are dependent on the concentration used and with participation of multiple histamine receptors.
- ItemAcesso aberto (Open Access)Histamina Hipocampal, Aprendizagem e Memória(Universidade Federal de São Paulo (UNIFESP), 2010) Goulart, Carolina de Souza [UNIFESP]; Medalha, Carla Christina [UNIFESP]; http://lattes.cnpq.br/1315289589167724; http://lattes.cnpq.br/7966349082379717; Universidade Federal de São Paulo (UNIFESP)A neuroplasticidade é a capacidade do SNC de alterar a sua estrutura com base nas experiências, responsável pelo mecanismo de aprendizagem. Estudos sugerem que drogas e neurotransmissores que participam dos processos de memória e aprendizagem são de fundamental importância para o entendimento do processo de recuperação após lesão de SNC. O presente estudo visa analisar as ações do sistema histaminérgico hipocampal sobre os processos de aquisição e consolidação da memória, em um teste de esquiva. Ratos Wistar machos foram implantados com cânulas nas regiões ventral e dorsal do hipocampo e treinados em um paradigma de esquiva inibitória. O animal foi individualmente colocado na plataforma, e no momento em que colocava as quatro patas no assoalho metálico, um choque foi liberado nas patas por 2s (0,4 mA; 40V). O procedimento era repetido até o rato permanecer 50s na plataforma. A latência de descida foi registrada e imediatamente após o treino os grupos de animais receberam o tratamento com histamina, salina (controle), coinfusão de histamina e antagonistas antagonistas receptores H1 (Pirilamina; 10,0 nmol/região) e H2 (Ranitidina; 20,0 nmol/região) no hipocampo dorsal ou ventral. Após 24 horas foi feito o teste no qual a evocação da memória foi testada por meio da repetição do procedimento de esquiva. A retenção da memória foi verificada através da latência, em segundos, do treino e do teste do animal da caixa de esquiva. Foi encontrado que a redução da atividade histaminérgica no hipocampo ventral, a partir da injeção intra de ranitidina foi capaz de reverter à tendência a extinção de memória aversiva propiciada pela histamina
- ItemAcesso aberto (Open Access)Histamine combined with melphalan in isolated limb perfusion for the treatment of locally advanced soft tissue sarcomas: preclinical studies in rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2005-08-01) Brunstein, Flavia [UNIFESP]; Santos, Ivan Dunshee de Abranches Oliveira [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Van Tiel, Sandra T.; Eggermont, Alexander M. M.; Ten Hagen, Timo L. M.; Universidade Federal de São Paulo (UNIFESP); Erasmus MC, Daniel den Hoed Cancer Center Surgical Oncology Department Laboratory of Experimental Surgery; Erasmus MC - Daniel den Hoed Cancer Center Surgical Oncology Department; Erasmus MC - Daniel den Hoed Cancer Center Surgical Oncology Department Laboratory of Experimental Surgical OncologyPURPOSE: To evaluate the potential benefit of histamine combined with melphalan in the isolated limb perfusion (ILP) as an alternative to TNF-alfa and melphalan combination, for the treatment of irressectable soft tissue sarcomas of the limbs in Brown Norway (BN) rats. METHODS: 20 BN rats had small fragments of syngeneic BN-175 fibosarcoma inserted on the right hind limb. In 7-10 days the tumor reached a median diameter of 12-15 mm and they were randomly divided in four groups (sham, melphalan, histamine and escalating doses of histamine combined to melphalan) being submitted to experimental ILP for 30 minutes. Tumors were measured daily with a caliper and the volume was calculated. RESULTS: Response curves showed a significant effect of the combination of histamine 200 mg/mL with melphalan, with 66% overall response, including 33% complete responses (p< 0.01). There were no systemic collateral effects and locally only mild temporary edema was observed for some animals treated with histamine. CONCLUSION: Histamine combined with melphalan had a promising effect in the ILP warranting future studies to better explore the mechanism of action as well as its potential use in organ perfusion.
- ItemAcesso aberto (Open Access)Histamine iontophoresis on the viability of random skin flap in rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2009-02-01) Esteves Junior, Ivaldo [UNIFESP]; Tacani, Pascale Mutti [UNIFESP]; Liggieri, Victor Cicone; Ruggi, Bruno Grinman; Ferreira, Lydia Masako [UNIFESP]; Liebano, Richard Eloin; UNIP Physical Therapy Department; Mackenzie Presbyterian University; Universidade Federal de São Paulo (UNIFESP); University of Sao Caetano do Sul Physical Therapy Department; Sao Camilo University Center; UNIP; Universidade de São Paulo (USP)PURPOSE: To evaluate the effects of the histamine iontophoresis on the random skin flap viability in rats. METHODS: Sixty adult male Wistar rats were used. A cranially-based dorsal skin flap measuring 10 x 4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surgical procedure, the animals were randomized into four groups (G1-G4) (n=15 each group) as follows: G1 (control) - sham electrical stimulation, G2 (electrical stimulation) - direct current electrical stimulation, G3 (histamine) - histamine and sham electrical stimulation and G4 (histamine iontophoresis) - transdermal iontophoresis of histamine. In all groups the procedures were performed immediately after the surgery and on the two subsequent days. The percentage of flap necrosis was measured on the seventh postoperative day. RESULTS: The mean and the respective standard deviation of the percentage of flap necrosis areas were as follows: G1 (control) - 47.87 ± 9.13%, G2 - 51.49 ± 8.19%, G3 - 46.33 ± 8.32% and G4 - 30.82 ± 11.25%. The G4 group presented a significantly smaller amount of flap necrosis when compared to the other groups (p<0.001). CONCLUSION: The topical administration of the histamine by iontophoresis was effective to increase the viability of the random skin flaps in rats.
- ItemSomente MetadadadosInflammatory events induced by brown spider venom and its recombinant dermonecrotic toxin: A pharmacological investigation(Elsevier B.V., 2009-04-01) Paludo, Katia Sabrina [UNIFESP]; Petris Biscaia, Stellee Marcela; Chaim, Olga Meiri [UNIFESP]; Otuki, Michel Fleith; Naliwaiko, Katya; Dombrowski, Patricia Andreia; Franco, Celia Regina Cavichiolo [UNIFESP]; Veiga, Silvio Sanches; Universidade Federal de São Paulo (UNIFESP); Univ Fed ParanaAccidents involving Brown spider (Loxosceles sp.) venom produce a massive inflammatory response in injured region. This venom has a complex mixture of different toxins, and the dermonecrotic toxin is the major contributor to toxic effects. the ability of Loxosceles intermedia venom and a recombinant isoform of dermonecrotic toxin to induce edema and increase in vascular permeability was investigated. These toxins were injected into hind paws and caused a marked dose and time-dependent edema and increase in vascular permeability in mice. Furthermore, the enzymatic activity of venom toxins may be primal for these effects. A mutated recombinant isoform of dermonecrotic toxin, that has only residual enzymatic activity, was not able to induce these inflammatory events. Besides the previous heating of toxins markedly reduced the paw edema and vascular permeability showing that thermolabile constituents can trigger these effects. in addition, the ability of these venom toxins to evoke inflammatory events was partially reduced in compound 48/80-pretreated animals, suggesting that mast cells may be involved in these responses. Pretreating mice with histamine (prometazine and cetirizine) and serotonin (methysergide) receptor antagonists significantly attenuated toxins induced edema and vascular permeability. Moreover, HPLC analysis of whole venom showed the presence of histamine sufficient to induce inflammatory responses. in conclusion, these inflammatory events may result from the activation of mast cells, which in turn release bioamines and may be related to intrinsic histamine content of venom. (C) 2008 Published by Elsevier Inc.
- ItemAcesso aberto (Open Access)Novas aril-metil-piperazinas da série LINS01 como ligantes de receptores de histamina H3R/H4R(Universidade Federal de São Paulo (UNIFESP), 2019-05-31) Nascimento, Lillian Ferreira Dos Santos [UNIFESP]; Fernandes, João Paulo dos Santos [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Histamine is an important biogenic amine distributed widely in all tissues. It plays an important role in the physiology and homeostasis of the organism, as well as in allergic and inflammatory processes. Its effects are observed through interaction with four histaminergic receptors G protein-coupled histaminergic receptors called H1, H2, H3 and H4, and the cell signaling that activates each receptor is distinct. The four histaminergic receptors are important targets for the treatment of various diseases. The first two receptors identified were already intensively researched and already have definite application in therapy. The H3 receptor has been characterized as auto and hetero-receptor and is found mostly in the central nervous system (CNS). It has thus become a promising target for the treatment of CNS disorders. Because it has been characterized later, the H4 receptor, present mainly in cells of hematopoietic origin, has been studied by several research groups as a target for the treatment of immunological and inflammatory diseases. Due to the homology between the H3 and H4 receptors many ligands may have affinity for both receptors. Thus, molecules with antagonistic or inverse agonist activity towards the H3 and/or H4 receptors demonstrate relevance in the treatment of several diseases involving alone or in combination these receptors. Therefore, the purpose of this work was to synthesize molecules based on the LINS 01 series with the highest yield and purity possible, to evaluate the affinity of these receptors for H3 and / or H4 receptors and to verify their activity as antagonist or agonist. Synthesis of the ligands involved steps such as allylation, Claisen rearrangement and reductive amination, resulting in intermediates which had crude yields of ~ 1 to 93%. The compounds designed in this work were not obtained due to the failure of chemical steps (low yield and / or no yield of the desired product) and purification. Thus, some intermediates obtained were tested through the binding test, but showed very low affinity in both receptors and thus it was not possible to evaluate their activities as antagonists or agonists in H3 and H4 receptors.
- ItemSomente MetadadadosRinometria acústica no monitoramento dos testes de provocação nasal com histamina em crianças e adolescentes com rinite alérgica(Universidade Federal de São Paulo (UNIFESP), 2008) Wandalsen, Gustavo Falbo [UNIFESP]; Solé, Dirceu [UNIFESP]Objetivo: padronizar e validar a rinometria acústica (RA) nos testes de provocação nasal (TPN) com histamina em crianças e adolescentes. Casuística e Métodos: foi realizada validação construtiva da RA, comparando-a a rinomanometria anterior ativa (RMAA). Vinte crianças e adolescentes com rinite alérgica persistente e 20 controles foram submetidos a TPN com histamina, monitorados pela RA e RMAA (SRE 2000, Rhinometrics). Os TPN foram realizados com instilação intranasal de soluções com concentrações crescentes de histamina (0,12 a 8,0 mg/ml). As variações na resistência nasal total (RNT, RMAA) foram comparadas as do volume dos cinco centímetros proximais da cavidade nasal (V5, RA). Os pacientes com rinite alérgica foram tratados com corticosteróide tópico nasal (furoato de mometasona, 100 μg/dia) por 21 dias, após os quais foram submetidos a novo TPN. Resultados: nas avaliações basais, os pacientes com rinite alérgica apresentaram valores de RNT significantemente superiores aos controles (média 0,34 Pa/cm3/s versus 0,21 Palcm3/s; p= 0,01) e inferiores de V5 (média 8,20 cm3 versus 9,24 cm3; p= 0,04). Houve diferença significante entre as concentrações de histamina capazes de induzir incremento de pelo menos 100% na RNT basal (média dos controles 2,4 mg/ml versus dos pacientes 0,72mg/ml, p< 0,001). Entre os pacientes com rinite alérgica, ao final do TPN, houve incremento médio de 126% na RNT, que correspondeu à redução média de 24,3% no V5. Com relação à queda do V5, 22% foi o ponto de corte co melhor balanço entre sensibilidade (72,5%) e especificidade (72,5%) para indicar a positividade do TPN..