Navegando por Palavras-chave "HLA-DR"

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    Analysis of ICAM-1 and HLA-DR expression on renal allograft aspirates
    (Munksgaard Int Publ Ltd, 1996-08-01) Manfro, Roberto Ceratti [UNIFESP]; Goncalves, LFS; Rauber, M.; Moura, Luiz Antonio Ribeiro de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    ICAM-1 and HLA-DR expressions were studied on parenchymal cells obtained by aspirative biopsies of renal allografts. Patients were evaluated during 3 months after transplantation. Conventional cytology and immunoperoxidase stainings using anti-ICAM-1 and anti-HLA-DR antibodies were employed. The value of the total corrected increment was significantly higher (p<0.05) during acute rejection episodes than during acute tubular necrosis or stable renal function, The percentage of HLA-DR positive cells were higher in rejection than in stable function periods (p<0.05). The percentage of ICAM-1 positive cells was higher in rejection than in acute tubular necrosis periods (p<0.05). The sensitivities to the diagnosis of rejection were 71.8% (TCI) 68.4% (ICAM-1) and 55.0% (HLA-DR). The specificities were 87.3%, 81.3% and 68.4% respectively. Combinations of the tests resulted in increased sensitivity. We concluded that the use of these monoclonal antibodies improves the sensitivity of conventional aspiration cytology to the diagnosis of acute renal rejection.
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    Conjunctival impression cytology evaluation of patients with dry eye disease using scleral contact lenses
    (Elsevier Science Bv, 2017) Weber, Sarah La Porta [UNIFESP]; Hazarbassanov, Rossen Mihaylov [UNIFESP]; Nasare, Alex [UNIFESP]; Pereira Gomes, Jose Alvaro [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]
    Purpose: To evaluate conjunctival impression cytology and HLADR expression changes after wearing scleral contact lenses (ScCLs) for moderate to severe dry eye disease (DED). Design: Prospective interventional case series. Methods: Forty-one eyes from 25 patients with moderate to severe DED were evaluated for Esclera ScCL treatment. Best-corrected visual acuity (BCVA) and slit-lamp findings were assessed. Impression cytology specimens were obtained from DED patients at the baseline and after wearing ScCLs for 12 months. The impression cytology specimens were analyzed using morphological results score, and HLADR positive cells were detected and quantified. The values were compared to assess the IC changes after wearing ScCLs. Results: Forty-one eyes from 25 patients were fitted with ScCLs to manage DED. The underlying diseases were Stevens-Johnson syndrome (22 eyes), Sjogren's syndrome (11 eyes), graft-versus-host disease (2 eyes), dry eye after keratomileusis (2 eyes) and undifferentiated ocular surface disease (4 eyes). The HEPAS impression cytology score did not differ significantly before and after wearing ScCLs for 12 months in DED patients (p > 0.05). The percentage of eyes expressing the HLA-DR antigen in the temporal conjunctiva after wearing ScCL for 12 months significantly increased in patients with Sjogren's syndrome (11.11% to 66.66%
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    Influence of EDTA and heparin on lipopolysaccharide binding and cell activation, evaluated at single-cell level in whole blood
    (Wiley-Blackwell, 2002-02-15) Brunialti, Milena Karina Coló [UNIFESP]; Kallas, Esper Georges [UNIFESP]; Freudenberg, M.; Galanos, C.; Salomão, Reinaldo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Max Planck Inst Immunbiol
    Background: the use of whole blood (WB) in studying lipopolysaccharide (LPS)-induced cellular activation preserves the milieu in which LPS-cell interaction occurs in vivo. However, little information is available on using such a system at a single-cell level. We evaluated LPS binding and cell activation in WB by using flow cytometry. the influence of heparin or EDTA as anticoagulants was also addressed. Methods: Blood was obtained from healthy donors in EDTA and/or heparin tubes. Biotinylated LPS (LPSb) was used to evaluate cell binding of LPS in WB. Cells were surface stained with appropriate antibodies and LPSb was detected by adding streptavidin-allophycocyanin (APC). LPS-induced activation was evaluated by the expression of surface activation markers and by the detection of intracellular tumor necrosis factor-alpha (TNF-alpha). Results: LPSb bound promptly to monocytes in EDTA- and heparin-treated blood. in EDTA-treated blood, membrane-bound LPSb decreased after 60 min of incubation, whereas it remained detectable in heparinized blood during the 6 h of incubation. LPS induced TNF-alpha and enhanced the expression of HLA-DR in monocytes, as well as the expression of CD69 in T and B lymphocytes. Induction of both TNF-alpha in monocytes and CD69 in lymphocytes was more efficient in heparinized blood. Conclusion: Detection of membrane-bound LPSb on monocytes differed in EDTA or heparin-treated blood, and cell activation was better obtained in heparinized blood. (C) 2002 Wiley-Liss, Inc.