Navegando por Palavras-chave "HEp-2 cells"

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    3º Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2 (FAN): recomendações para padronização do ensaio de pesquisa de autoanticorpos em células HEp-2, controle de qualidade e associações clínicas
    (Sociedade Brasileira de Reumatologia, 2009-04-01) Dellavance, Alessandra [UNIFESP]; Gabriel Júnior, Alexandre [UNIFESP]; Nuccitelli, Barbara; Taliberti, Ben Hur; Von Mühlen, Carlos Alberto; Bichara, Carlos David Araújo; Santos, Cláudio Henrique Ramos dos; Bueno, Cleonice; Yano, Cristiane Martinez; Mangueira, Cristóvão Luis Pitangueiras; Carvalho, Darlene Gonçalves; Cardoso, Elizângela; Bonfá, Eloisa Silva Dutra de Oliveira; Araújo, Flávia Ikeda e; Rassi, Gustavo Gabriel; Mundim, Hugo Mendonça; Bendet, Izidro; Rego, Jozelia; Vieira, Lisiane Maria Enriconi dos Anjos; Andrade, Luiz Eduardo Coelho [UNIFESP]; Barbosa, Maria Ordália Ferro; Sugiyama, Mitiko; Santiago, Mittermayer Barreto; Slhessarenko, Natasha; Silva, Nilzio Antônio da; Francescantonio, Paulo Luiz Carvalho; Jarach, Renata; Suda, Roberto; Levy, Roger Abramino; Sampaio, Silvia Oliveira; Neves, Suzane Pretti Figueiredo; Cruvinel, Wilson de Melo [UNIFESP]; Santos, Wilton Silva dos [UNIFESP]; Nóbrega, Yanna Karla de Medeiros; Universidade Federal de São Paulo (UNIFESP); Fleury Medicina Diagnóstica Setor de Imunologia; AFIP-Medicina Laboratorial; Centro Imuno-Reumatológico de São Paulo; Padrão Laboratório Clínico; Universidade Federal de Uberlândia; HC UFU; PUCRS Faculdade de Medicina; UFPA; Laboratório Amaral Costa; CESEP/PA; NewLife Comércio de Produtos Laboratoriais; Universidade de São Paulo (USP); Universidade Católica de Goiás; Hospital Israelita Albert Einstein; Instituto Hermes Pardini; Biometrix Diagnóstica; UCG Departamento de Biomedicina; Laboratório Atalaia; Sérgio Franco Medicina Diagnóstica Setor de Imunoensaios; Universidade Federal de Goiás Faculdade de Medicina Hospital das Clínicas; Universidade do Sul de Santa Catarina; Universidade do Vale do Itajaí; Laboratório Médico Santa Luzia; Hospital e Maternidade e Jardim América Laboratórios Saúde; Hemagen Diagnósticos; Universidade Federal da Bahia; Fundação Bahiana para Desenvolvimento das Ciências; Hospital Santa Izabel; Universidade Federal de Mato Grosso; Universidade Federal de Goiás Faculdade de Medicina; Alka; Universidade do Estado do Rio de Janeiro; UERJ; Diagnósticos da América SA; Universidade Federal de Minas Gerais Faculdade de Medicina; Hospital Universitário de Brasília; União Educacional do Planalto Central; Centro Universitário Unieuro; Imunotech Sistemas Diagnósticos Importação e Exportação
    OBJECTIVE: The Third Brazilian Consensus for autoantibodies Screening in HEp-2 cells had as purpose the evaluation of difficulties in the accomplishment of the 2nd Consensus recommendations that took place in the year of 2002, the discussion of strategies for quality control of the assay and the promotion of an update of the clinical associations of the several immunofluorescent patterns. METHODS:Several ANA experts from university centers and private laboratories in different areas in Brazil joined the workshop in Goiânia on 2008 April 13 and 14 with the purpose of discussing and approving the recommendations for standardization, interpretation and use of the test by physicians. Commercial representatives of different ANA slide brands were also invited as listeners to the workshop. RESULTS AND CONCLUSIONS: The 3rd Consensus emphasized the need for quality control in indirect immunofluorescent since there is a considerable heterogeneity of available microscopes and reagents. It also promoted adaptations in the previously approved terminology used to classify the different patterns and finally updated the clinical associations of the several patterns with the purpose of providing guidance for interpretation of the assay by clinical pathologists and assistant physicians.
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    Análise crítica do teste de anticorpos antinúcleo (FAn) na prática clínica
    (Sociedade Brasileira de Reumatologia, 2007-08-01) Dellavance, Alessandra [UNIFESP]; Leser, Paulo Guilherme; Andrade, Luiz Eduardo Coelho [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Fleury Medicina Diagnóstica Setor de Imunologia
    Due to its continuous evolving process the fluorescent antinuclear antibody test (ANA-HEp-2) has required constant update efforts from personal involved in performing and interpreting its results. The methodological advances have brought up a considerable improvement in the test's sensitivity and consequently a decrease in its specificity. This has resulted in an increasing number of positive tests in apparently healthy subjects. This fact contributes to embarrassing situations and implies in the necessity to seek for elements to help the involved professionals. Well-conducted studies and experience have shown that there are some peculiar features associated with the auto-antibodies in patients with autoimmune diseases that are not present in those observed in healthy subjects. The present review brings an approach on the most important points to be considered in the analysis and evaluation of an ANA test that might help the identification of patients with autoimmune disease. Titer and immunofluorescence pattern are important parameters in the evaluation of the significance of a positive ANA-HEp2 test and in the reflex demand for further tests for specific autoantibodies. In general non-autoimmune individuals present low titer ANA-HEp-2 and patients with systemic autoimmune diseases present moder ate or high titer ANA-HEp-2 tests. However, exceptions to this are normally observed in both contexts. The homogeneous and coarse speckled nuclear patterns are almost exclusively observed in patients with systemic autoimmunity. On the other hand, the nuclear dense fine speckled pattern is one of the most frequently observed patterns in non-autoimmune individuals with a positive ANA-HEp-2 test. The basic concepts of the National Consensus on Standardization of ANA-HEp-2 Report have helped Brazilian rheumatologists and pathologists in the correct interpretation of the great diversity of ANA-HEp-2 immunofluorescence patterns. Finally, it is important to consider the possible and alternative significance of a positive ANA test in patients without objective evidence of autoimmune diseases.
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    Como interpretar e valorizar adequadamente o teste de anticorpos antinúcleo
    (Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia, 2007-06-01) Dellavance, Alessandra [UNIFESP]; Andrade, Luiz Eduardo Coelho [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Fleury Medicina Diagnóstica Setor de Imunologia
    The tradicional fluorescent antinuclear antibody test (ANA) has required constant update efforts from personel involved in performing and interpreting its results. The methodological advances have brought up a considerable improvement in the test s sensitivity and, consequentely, a decrease in its specificity. This has resulted in an increasing number of positive tests in apparently healthy subjects. However, there are some peculiar features associated with the auto-antibodies in patients with autoimmune diseases that are not present in those observed in healthy subjects. The objective of the present review is to bring an approach on the most important points to be considered in the analysis and evalution of an ANA test that might help in the identification of patients with autoimmune disease. Title and immunofluorescence pattern are discussed as important parameters and they are important in the evaluation of ANA and in the reflex demand for further tests for specific autoantibodies. The basic concepts of the National Consensus on Standardization of ANA-HEp-2 Report are posted. Finally, we explore the possible meanings of a positive ANA test in a patient without objective evidence of autoimmune disease.
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    II Consenso Brasileiro de Fator Antinuclear em Células HEp-2: Definitions for standardization of autoantibody testing against the nucleus (ANA HEp-2), nucleolus, cytoplasm and mitotic apparatus, as wel as its clinical associations
    (Sociedade Brasileira de Reumatologia, 2003-06-01) Dellavance, Alessandra [UNIFESP]; Gabriel Júnior, Alexandre [UNIFESP]; Cintra, Alice Friedenberg de Ulhôa; Ximenes, Antônio Carlos; Nuccitelli, Barbara; Taliberti, Ben Hur; Moreira, Caio; Von Mühlen, Carlos Alberto; Bichara, Carlos David Araújo; Santos, Cláudio Henrique Ramos dos; Yano, Cristiane Martinez; Mangueira, Cristóvão Luis Pitangueiras; Carvalho, Darlene Gonçalves; Bonfá, Eloisa Silva Dutra de Oliveira; Doi, Elvira M.; Guimarães, Fabiana Nunes de Carvalho; Araújo, Flávia Ikeda e; Mundim, Hugo Mendonça; Rego, Jozelia; Vieira, Lisiane Ericonio dos Anjos; Poli, Luciana; Andrade, Luiz Eduardo Coelho [UNIFESP]; Callado, Maria Roseli; Mesquita, Mauro Meira; Sugiyama, Mitiko; Slhessarenko, Natasha; Silva, Nilzio Antônio da; Carballo, Orlando Gabriel; Leser, Paulo Guilherme [UNIFESP]; Francescantonio, Paulo Luiz Carvalho; Jarach, Renata; Xavier, Ricardo Machado; Levy, Roger Abramino; Neves, Suzane Pretti Figueiredo; Cruvinel, Wilson de Melo [UNIFESP]; Santos, Wilton Silva dos [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Bio-Rad Laboratório Brasil; Hospital Geral de Goiânia; Biomédica; Universidade Federal de Uberlândia; UFMG HC; PUCRS HC; New Life Produtos Hospitalares; Universidade Católica de Goiás; Patologista Clínica; Frischmann Aisengart Unidad Inmunología; Universidade Católica de Goiás Laboratório de Auto-Imunidade; Exame Medicina Laboratorial; FMUFG HC Laboratório de Imuno-Reumatologia e HLA; Lab. Santa Luzia; Medivax/Bion; HSPE/SP; Universidade Católica de Goiás Laboratório da Área de Saúde; Farmacêutica-Bioquímica; Univ. Fed. Mato Grosso; FMUFG Serviço de Reumatologia; Hospital Durand Unidad Inmunología; Laboratório Clínico; UFRGS HCPA Serviço de Reumatologia; UERJ FCM; UFMG FM; Hospital Universitário de Brasília Laboratório de Reumatologia
    OBJECTIVE: The Second Brazilian Consensus on Antinuclear Antibodies (ANA) in HEp-2 Cells approved and extended the decision trees developed during the First Brazilian Consensus in order to also offer information about mixed patterns of fluorescence. METHODS: Since this test elicits reactions not only to nuclear autoimmune antigens but also to different cell compartments, new denominations for the test were approved. Results and CONCLUSIONS: These new denominations encompass variations on the autoantibody testing against the nucleus (ANA HEp-2), nucleolus, cytoplasm, and mitotic apparatus issue. Furthermore, major clinical associations were described for each immunofluorescent pattern, facilitating the interpretation of laboratory results in the clinical practice.
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    III Consenso Brasileiro para Pesquisa de Autoanticorpos em Células HEp-2: perspectiva histórica, controle de qualidade e associações clínicas
    (Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia, 2009-06-01) Francescantonio, Paulo Luiz Carvalho; Andrade, Luiz Eduardo Coelho [UNIFESP]; Cruvinel, Wilson de Melo [UNIFESP]; Araújo, Flávia Ikeda e; Dellavance, Alessandra [UNIFESP]; Gabriel Júnior, Alexandre [UNIFESP]; Nuccitelli, Barbara; Taliberti, Ben Hur; Von Mühlen, Carlos Alberto; Bichara, Carlos David Araújo; Santos, Cláudio Henrique Ramos dos; Bueno, Cleonice; Yano, Cristiane Martinez; Mangueira, Cristóvão Luis Pitangueiras; Carvalho, Darlene Gonçalves; Cardoso, Elizângela; Bonfá, Eloisa Silva Dutra de Oliveira; Rassi, Gustavo Gabriel; Mundim, Hugo Mendonça; Bendet, Izidro; Rego, Jozelia; Vieira, Lisiane Maria Enriconi dos Anjos; Barbosa, Maria Ordália Ferro; Sugiyama, Mitiko; Santiago, Mittermayer Barreto; Slhessarenko, Natasha; Silva, Nilzio Antônio da; Jarach, Renata; Suda, Roberto; Levy, Roger Abramino; Sampaio, Silvia Oliveira; Neves, Suzane Pretti Figueiredo; Santos, Wilton Silva dos [UNIFESP]; Nóbrega, Yanna Karla de Medeiros; UCG; Universidade Federal de São Paulo (UNIFESP); Fleury Medicina e Saúde setor de Imunologia; UCG Departamento de Biomedicina Laboratório de Apoio Didático; Centro Imuno-Reumatológico de São Paulo AFIP-Medicina Laboratorial; Padrão Laboratório Clínico; Universidade Federal de Uberlândia; UFU Hospital das Clínicas serviço de reumatologia; Pontifícia Universidade do Rio Grande do Sul Faculdade de Medicina; Metanalysis Centro de Diagnósticos Médicos; Universidade Federal do Pará; Laboratório Amaral Costa; Centro de Educação Técnica do Estado do Pará; NewLife Comércio de Produtos Laboratoriais; Universidade de São Paulo (USP); Hospital Israelita Albert Einstein Departamento de Patologia Clínica; Instituto Hermes Pardini; Biometrix Diagnóstica; Laboratório Atalaia; Sérgio Franco Medicina Diagnóstica setor de Imunoensaios; UCG Faculdade de Medicina Hospital das Clínicas; Universidade do Sul de Santa Catarina; Universidade do Vale do Itajaí; Laboratório Médico Santa Luzia; Hospital e Maternidade e Jardim América Laboratórios Saúde; Hemagen Diagnósticos; Universidade Federal da Bahia; Fundação Bahiana para Desenvolvimento das Ciências; Hospital Santa Izabel serviço de reumatologia; Universidade Federal de Mato Grosso; FMUCG; Alka; Universidade do Estado do Rio de Janeiro; Hospital Pró-cardíaco Centro de Autoimunidades; Diagnósticos da América SA; Laboratório Diagnósticos da América SA setor de Imunologia; Universidade Federal de Minas Gerais Faculdade de Medicina; UFMG Hospital das Clínicas Serviço de Medicina Laboratorial; Hospital Universitário de Brasília Laboratório de Reumatologia; União Educacional do Planalto Central; Centro Universitário Euro-Americano; Imunotech Sistemas Diagnósticos Importação e Exportação
    OBJECTIVE: The Third Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) had as purpose the evaluation of difficulties in the accomplishment of the 2nd Consensus recommendations that took place in the year of 2002, the discussion of strategies for quality control of the assay and the discussion of an update of the clinical associations of the several immunofluorescent patterns. METHODS: Several ANA experts from university centers and private laboratories in different areas in Brazil joined the workshop in Goiânia on 2007 April 13 and 14 with the purpose of discussing and approving the recommendations for standardization, interpretation and use of the test by physicians. Commercial representatives of different ANA slide brands were also invited as listeners to the workshop. RESULTS AND CONCLUSION: The 3rd ANA Consensus emphasized the need for quality control in indirect immunofluorescent assays since there is a considerable heterogeneity of available microscopes and reagents. It also promoted adaptations in the previously approved terminology used to classify the different patterns and finally updated the clinical associations of the several patterns with the purpose of providing guidance for interpretation of the assay by clinical pathologists and assistant physicians.
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    IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
    (Elsevier B.V., 2014-01-01) Francescantonio, Paulo Luiz Carvalho; Cruvinel, Wilson de Melo [UNIFESP]; Dellavance, Alessandra [UNIFESP]; Andrade, Luiz Eduardo Coelho [UNIFESP]; Taliberti, Ben Hur; Von Mühlen, Carlos Alberto; Bichara, Carlos David Araújo; Bueno, Cleonice; Mangueira, Cristóvão Luis Pitangueiras; Carvalho, Darlene Gonçalves; Bonfá, Eloisa Silva Dutra de Oliveira; Brito, Fabiano de Almeida; Araújo, Flávia Ikeda e; Rego, Jozelia; Pereira, Kaline Medeiros Costa; Anjos, Lisiane Maria Enriconi dos; Bissoli, Maria de Fatima; Santiago, Mittermayer Barreto; Maluf, Natalya Zaidan; Alvarenga, Rossana Rassi; Neves, Suzane Pretti Figueiredo; Valim, Valeria; Santos, Wilton Silva dos [UNIFESP]; PUC Goias; Fleury Med & Saude; Universidade Federal de São Paulo (UNIFESP); Universidade Federal de Uberlândia (UFU); Clin Doencas Reumat Porto Alegre; Amaral Costa Med Diagnost; Universidade de São Paulo (USP); Hosp Israelita Albert Einstein; Inst Hermes Pardini; Universidade Federal de Minas Gerais (UFMG); Univ Catolica Brasilia; Universidade Federal de Goiás (UFG); Univ Sul Santa Catarina UNISUL; Univ Vale do Itajai UNIVALE; Univ Fed Espirito Santo; EBMSP; Grp DASA; Escola Super Ciencias Saude Dist Fed; Lab Sabin
    Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved.