Navegando por Palavras-chave "Genetic damage"
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- ItemSomente MetadadadosAcute Spinal Cord Injury Induces Genetic Damage in Multiple Organs of Rats(Springer, 2012-08-01) Medalha, Carla Christina [UNIFESP]; Polesel, Fernanda Sato [UNIFESP]; Silva, Victor Hugo Pereira da [UNIFESP]; Martins, Renato Almeida [UNIFESP]; Pozzi, Renan [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Spinal cord injury (SCI) is a devastating condition with important functional and psychological consequences. However, the underlying mechanisms by which these alterations occur are still not fully understood. the aim of this study was to analyze genomic instability in multiple organs in the acute phase of SCI by means of single cell gel (comet) assay. Rats were randomly distributed into two groups (n = 5): a SHAM and a SCI group killed 24 h after cord transection surgery. the results pointed out genetic damage in blood cells as depicted by the tail moment results. DNA breakage was also detected in liver and kidney cells after SCI. Taken together, our results suggest that SCI induces genomic damage in multiple organs of Wistar rats.
- ItemSomente MetadadadosAvaliação de genotoxicidade em múltiplos órgãos após compressão dos membros inferiores de ratos(Universidade Federal de São Paulo (UNIFESP), 2014-10-14) Carneiro Neto, Nicola Jorge [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Muscle injury is a common trauma in modern society as a result of mass disasters, muscle penetration by high velocity projectiles, blunt external trauma, or by prolonged immobilization in comatose patients after head trauma, alcoholic or drug overdose. However, the underlying mechanisms linking these alterations are still not fully understood, especially in acute phase. The aim of this study was to analyze genomic instability in multiple organs of rats after acute muscle injury by means of single cell gel (comet) assay. Rats were randomly distributed into three groups (n = 6 each group): control group and experimental groups: sacrificed 6 h as 12 h after muscle compression. The results indicate genetic damage in peripheral blood cells as depicted by tail moment results. DNA breakage was also detected in liver, lung and kidney cells after acute muscle injury for two times evaluated. Heart cells showed genetic damage after 12 h following muscle compression. Taken together, our results suggest that acute muscle injury induces genomic damage in multiple organs of Wistar rats. This novel finding offers new insights into the underlying mechanisms of the relationship between acute crush muscle injury and clinical manifestations that can occur during limb compression.
- ItemSomente MetadadadosCytogenetic damage induced by mouthrinses formulations in vivo and in vitro(Springer, 2012-06-01) Carlin, Viviane [UNIFESP]; Matsumoto, Mariza A.; Saraiva, Patricia P.; Artioli, Andre; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); USCThe aim of the present study was to comparatively evaluate DNA damage and cellular death in cells exposed to various commercially available mouthrinses: ListerineA (R) CepacolA (R), Plax alcohol freeA (R), PeriogardA (R), and Plax WhiteningA (R). A total of 75 volunteers were included in the search distributed into five groups containing 15 people each for in vivo study. Exfoliated buccal mucosa cells were collected immediately before mouthrinse exposure and after 2 weeks. Furthermore, blood samples were obtained from three healthy donors for in vitro study. the micronucleus test was used to evaluate mutagenicity and cytotoxicity in vivo. the single-cell gel (comet) assay was used to determine DNA damage in vitro. After 2 weeks exposure, PeriogardA (R) showed 1.8% of micronucleated cells with significant statistical differences ( < 0.05) compared to before exposure (0.27%). Plax WhiteningA (R) presented high tail moment value (4.5) when compared to negative control (0.6). the addition of all mouthrinses to cells incubated with methyl methanesulfonate did not alter the number of strand breaks in the genetic material. ListerineA (R) was able to reduce genetic damage induced by hydrogen peroxide because a decrease of tail moment was noticed. the results of the present study suggest that PeriogardA (R) and Plax WhiteningA (R) can induce genetic damage, whereas ListerineA (R) is an antioxidant agent. Since DNA damage is considered to be prime mechanism during chemical carcinogenesis, these data may be relevant in risk assessment for protecting human health and preventing carcinogenesis.
- ItemSomente MetadadadosSystemic Host Response Following Skin Burn Injury in Rats: Cytotoxicity and Genotoxicity Evaluation(Int Inst Anticancer Research, 2014-11-01) Quintana, Hananiah Tardivo [UNIFESP]; Bortolin, Jeferson André [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; De Oliveira, Flavia [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Aim: The aim of the present study was to investigate whether skin burn injury (BI) can induce cellular changes in skeletal muscle, liver, kidney and blood by means of DNA damage and cytotoxicity. Materials and Methods: Thirty male Wistar rats were distributed into two groups: control (C) and submitted to scald burn (SB), subdivided into three subgroups: 1, 4 or 14 days post-injury. The gastrocnemius muscle and liver were dissected for histopathological evaluation and the single-cell gel (comet) assay was used to investigate damage in skeletal muscle, liver, kidney and blood cells. Results: Histopathological analysis of the muscle in the SB group revealed congested vessels containing inflammatory cells for all periods evaluated post-injury. In liver, the one day post-injury SB group showed sinusoidal congestion, while that of 14 days post-injury exhibited an increased number of Kupffer cells. Conclusion: Despite the histophatological evidence, none of the groups showed any signs of genotoxicity in these target tissues.