Navegando por Palavras-chave "GLYCOSAMINOGLYCANS"
Agora exibindo 1 - 7 de 7
Resultados por página
Opções de Ordenação
- ItemSomente MetadadadosAnomalous structure of urinary chondroitin sulfate from cancer patients. A potential new marker for diagnosis of neoplasias(Williams & Wilkins, 1993-04-01) Dietrich, Carl Peter [UNIFESP]; Martins, João Roberto Maciel [UNIFESP]; Sampaio, Lucia de Oliveira [UNIFESP]; Nader, Helena Bonciani [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)BACKGROUND: Chondroitin sulfate is significantly increased in tumors (10 to 100 times) when compared to the amounts present in normal adjacent tissues. To investigate if the changes in concentration of chondroitin sulfate could be reflected in the urine of cancer patients we have analyzed the chondroitin sulfate excreted by 44 patients with different types of tumors, 50 normal individuals and 15 patients with unrelated diseases.EXPERIMENTAL DESIGN: The identification and structural analyses of the sulfated glycosaminoglycans were made by electrophoresis and degradation with specific enzymes (chondroitinases AC and ABC), identification/quantitation of their disaccharide products by chromatography (paper and HPLC) and chemical determinations.RESULTS: The disaccharide products formed from chondroitin sulfate of the 44 cancer patients by action of chondroitinase ABC show a substantial relative increase of non sulfated disaccharide (32.1% +/- 15.2) with a relative decrease of 6-sulfated disaccharide (28.9% +/- 11.5) and 4-sulfated disaccharide (39.0% +/- 13.5) when compared to the chondroitin sulfate of normal subjects (9.1% +/- 2.2, 40.6% +/- 4.5 and 50.2% +/- 4.5, respectively) or from patients with unrelated diseases. There is a direct correlation between the non sulfated disaccharide content and the stage of malignancy of the cancer patients. A significant change of the ratio of chondroitin sulfate and heparan sulfate and a decrease in the electrophoretic migration of chondroitin sulfate were also observed in cancer patients.CONCLUSIONS: All the cancer patients analyzed so far have shown the structural anomaly of the urinary chondroitin sulfate and this may be useful in the diagnosis and follow up of cancer therapy.
- ItemSomente MetadadadosBIOSYNTHESIS OF GLYCOSAMINOGLYCANS IN THE ENDOMETRIUM DURING THE INITIAL-STAGES OF PREGNANCY OF THE MOUSE(Cellular & Molecular Biology, 1995-02-01) Zorn, TMT; Pinhal, Maria Aparecida da Silva [UNIFESP]; Nader, Helena Bonciani [UNIFESP]; Carvalho, J. J.; Abrahamsohn, P. A.; Dietrich, Carl Peter [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Significant changes in the synthesis of glycosaminoglycans occur during the transformation of stromal cells of the endometrium into decidual cells which takes place during the initial stages of pregnancy in mice. Hyaluronic acid, which is practically absent in the endometrium of virgin mice, increases dramatically on the fifth day of pregnancy, reaching its maximal concentration on day 6 followed by a 50% decrease on day 7. Changes in hyaluronic acid concentration also occur in pseudopregnant mice indicating that they are not related to the presence of the embryo in the uterus. The absolute concentration of the sulfated glycosaminoglycans, e.g., heparan sulfate, dermatan sulfate and chondroitin sulfate in the decidua did not change significantly. There was, however, a striking decrease of their biosynthesis in pregnant and pseudopregnant mice when compared to virgin mice, as shown by the use of radioactive inorganic sulfate as a precursor for the study of in vivo synthesis. A radioautographical analysis confirmed that the highest incorporation of radioactive sulfate was observed in virgin endometria when compared to pregnant ones. These studies also have shown a characteristic pattern of labeling in different regions of the endometrium that repeats itself during the different days of pregnancy.
- ItemSomente MetadadadosEFFECT of DIFFERENT GLYCOSAMINOGLYCANS in A GUINEA-PIG CAROTID-ARTERY THROMBOSIS MODEL(Elsevier B.V., 1994-09-15) Mattar, L.; Maffei, FHA; Nader, H. B.; Dietrich, C. P.; Curi, P. R.; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)Heparin is the most frequently used drug for the prevention and treatment of thrombosis. Its use, however, is restricted by its side-effects. To study the efficacy of other glycosaminoglycans that could substitute heparin in the management of arterial thrombosis, 60 guinea-pigs were randomly allocated into 6 groups: G1= control, G2= heparin (150 IU/kg), G3= heparan sulfate from beef pancreas (2.5 mg/kg), G4= heparan sulfate from beef lung (2.5 mg/kg), G5= N-acetylated heparan from beef pancreas, G6= dermatan sulfate from beef intestine (2.5 mg/kg). Ten minutes after intravenous injection of the drugs, thrombosis was induced by the injection of a 50% glucose solution into a segment of the right carotid artery isolated between 2 thread loops during 10 minutes. Three hours later the artery was re-exposed and if a thrombus was present it was measured, withdrawn and weighed. Thrombin time and activated partial thromboplastin time were measured in all animals. Thrombus developed in 90% of the animals in the control group, 0% in G2 and G3, 62.5% in G4, 87.5% in G5 and G6. Only in the animals treated with heparin the coagulation tests were prolonged. in conclusion, in the used dose only the heparan sulfate from beef pancreas presented an antithrombotic effect similar to heparin in this experimental model.
- ItemSomente MetadadadosEffects of different doses of soy isoflavones on bone tissue of ovariectomized rats(Informa Healthcare, 2014-08-01) Santos, M. A. [UNIFESP]; Florencio-Silva, R. [UNIFESP]; Medeiros, V. P. [UNIFESP]; Nader, H. B. [UNIFESP]; Nonaka, K. O.; Sasso, G. R. S. [UNIFESP]; Simoes, M. J. [UNIFESP]; Reginato, R. D. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal de São Carlos (UFSCar)Aim Studies report that hormone replacement prevents osteoporosis, but there are doubts whether isoflavones are really efficient in this process. the aim of this study was to evaluate the effects of different doses of soy isoflavones on bone tissue of ovariectomized rats.Methods Forty female rats at the age of 6 months were ovariectomized and, after 3 months, the animals were divided into four groups: GI - Control (treated with drug vehicle); GII - treated with isoflavones (80 mg/kg per day); GIII - treated with isoflavones (200 mg/kg per day) and GIV - treated with isoflavones (350 mg/kg per day). Soy isoflavones were administered by gavage for 90 consecutive days. After treatment, the rats were euthanized and their distal femurs were removed for histological routine, histochemistry and biochemical study. Histological sections were stained with hematoxylin-eosin or subjected to picrosirius red and alcian blue methods. Shafts of femurs were submitted to biochemical assay and tibias were subjected to biophysical and biomechanical tests.Results in distal femurs, the trabecular bone volume was higher in the groups treated with isoflavones, being higher in GIV, while the cortical bone width and the presence of mature type I collagen fibers were higher in GII. At the trabecular bone region, the percentage of total glycosaminoglycans (GAGs) was higher in GII and the percentage of only sulfated GAGs was higher in Gill, while the higher content of chondroitin sulfate in shafts of femurs was seen in GIV. Biophysical and biomechanical tests in tibias did not differ among the groups.Conclusion Our data indicate that soy isoflavones improve bone quality in femurs of rats by increasing histomorphometric parameters, the content of GAGs and mature type I collagen fibers. These positive effects are dose-dependent and it was different in cortical and trabecular bone.
- ItemSomente MetadadadosEffects of soy isoflavones and mechanical vibration on rat bone tissue(Informa Healthcare, 2013-12-01) Florencio-Silva, R. [UNIFESP]; Santos, M. A. [UNIFESP]; Medeiros, V. P. de [UNIFESP]; Nader, H. B. [UNIFESP]; Nonaka, K. O.; Simoes, M. J. [UNIFESP]; Reginato, R. D. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal de São Carlos (UFSCar)Objective To investigate the effects of soy isoflavones (Iso) and mechanical vibration treatments alone or combined on bone extracellular matrix constituents of ovariectomized rats.Methods Forty female Wistar rats at the age of 6 months were ovariectomized (Ovx) and ten were sham-operated (sham). After 3 months, the animals were divided into five groups: GI (sham); GII (Ovx); GIII, ovariectomized and orally treated with isoflavones (200 mg/kg) for 90 consecutive days; GIV, ovariectomized and submitted to vibration for 90 days (5 days/week); GV, ovariectomized and treated with isoflavones plus vibration. After treatments, the rats were euthanized, and their femurs were removed for histological routine and biochemical study. Histological sections were stained with hematoxylin-eosin, picrosirius red and alcian blue. Shaft of femurs were submitted to biochemical assay and tibias were subjected to biophysical and biomechanical tests.Results Treatments did not have significant effects on the trabecular bone volume, but the combined treatments showed trophic effects on the cortical bone width and area. Bone density and the content of organic material of the tibias were higher in the GIV and GV groups. the GV group showed the highest presence of mature collagen fibers and content of total glycosaminoglycans, while the highest contents of chondroitin sulfate and other sulfated glycosaminoglycans were seen in the GIV group.Conclusion the mechanical vibration treatment is more efficient than soy isoflavones in improving bone quality by increasing the bone density, the content of sulfated glycosaminoglycans and the presence of mature collagen fibers. in addition, the combined interventions have partial trophic and synergistic effects that are bone site-specific in ovariectomized rats.
- ItemSomente MetadadadosGLYCOSAMINOGLYCAN DISTRIBUTION in ATHEROSCLEROTIC SAPHENOUS-VEIN GRAFTS(Karger, 1995-03-01) Marquezini, M. V.; Strunz, CMC; Dallan, LAO; Toledo, OMS; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)Glycosaminoglycan composition of normal saphenous veins and atherosclerotic saphenous vein grafts is reported. Dermatan sulfate is the main glycosaminoglycan present in both normal saphenous veins and saphenous vein grafts. These tissues also contain chondroitin sulfate and heparan sulfate. Although the total amount of glycosaminoglycans decreased in the grafts (compared with normal saphenous veins), the grafts showed an increase in the relative amounts of dermatan sulfate and chondroitin sulfate. Heparan sulfate was decreased, compared with normal controls. These findings suggest the involvement of blood vessel glycosaminoglycans (not only the arterial glycosaminoglycans) in the process of atherosclerosis.
- ItemSomente MetadadadosUterine prolapse: evaluation of glycosaminoglycans in postmenopausal women after estrogen therapy(Informa Healthcare, 2011-02-01) Cavalcante Nunes, J. M. V. [UNIFESP]; Cezar Feldner, P. [UNIFESP]; Aquino Castro, R. [UNIFESP]; Nader, H. B. [UNIFESP]; Ferreira Sartori, M. G. [UNIFESP]; Batista Castello Girao, M. J. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective To evaluate glycosaminoglycans (GAGs) in the parametrium, paraurethral tissue and vaginal apex in postmenopausal women with uterine prolapse and to evaluate the effect of 30-day estrogen therapy in these patients.Material and methods Double-blind trial of estrogen and placebo in 40 women with a control group of 20 premenopausal women without uterine prolapse. Twenty postmenopausal women with prolapse formed a second group and were treated with placebo for 30 days before vaginal hysterectomy. the third group included 20 postmenopausal women with prolapse who received 0.625 mg oral conjugated estrogens for 30 days before vaginal hysterectomy. Samples of the parametrium, vaginal apex and paraurethral tissue were obtained during surgery.Results Hyaluronic acid was the predominant GAG detected, followed by dermatan sulfate, chondroitin sulfate and heparan sulfate. in postmenopausal women with prolapse, we did not observe significant differences in total GAGs compared to the control group. However, hyaluronic acid was increased in the parametrium of women receiving estrogen compared to those treated with the placebo (2033.39 +/- 3037.90 mg/g vs. 587.87 +/- 697.89 mg/g, respectively; p = 0.041).Conclusions There are differences in GAGs in the parametrium, paraurethral tissue and vaginal apex between women in premenopause and those in the postmenopause period. Therefore, 30-day estrogen therapy produces significant differences in levels of hyaluronic acid, dermatan sulfate and chondroitin sulfate.