Navegando por Palavras-chave "Experimental endometriosis"

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    Does gestrinone antagonize the effects of estrogen on endometrial implants upon the peritoneum of rats?
    (Faculdade de Medicina / USP, 2008-01-01) Lobo, Vera Lúcia Rodrigues [UNIFESP]; Soares Júnior, José Maria [UNIFESP]; Simões, Manuel de Jesus [UNIFESP]; Simões, Ricardo dos Santos [UNIFESP]; Lima, Geraldo Rodrigues de [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    OBJECTIVE: To evaluate the effects of estrogen treatment in combination with gestrinone on an experimental rat model of endometriosis. METHODS: Uterine transplants were attached to the peritoneum of female Wistar rats via a surgical autotransplantation technique. The implanted area was measured during the proestrus phase and after hormonal treatment. We performed morphometric analysis and examined the macroscopic and morphometric alterations of endometrial implants after hormonal treatment in ovariectomized rats. RESULTS: The high dose of estrogen caused macroscopic increases in the endometrial implant group compared with other groups, which were similar to increases in the proestrus phase. The low dose showed morphometric development of implants, such as an increase in number of endometrial glands, leukocyte infiltration and mitosis. Gestrinone antagonized both doses of estrogen. CONCLUSION: Our findings suggest that gestrinone antagonizes estrogen's effects on rat peritoneal endometrial implants.
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    Experimental endometriosis reduction in rats treated with Uncaria tomentosa (cat's claw) extract
    (Elsevier B.V., 2011-02-01) Nogueira Neto, Joao [UNIFESP]; Coelho, Tarcisio Mota; Aguiar, Guilherme Carneiro; Carvalho, Laura Rosa; Portela de Araujo, Ana Giselia; Girao, Manuel Joao B. C. [UNIFESP]; Schor, Eduardo [UNIFESP]; Univ Fed Maranhao; Universidade Federal de São Paulo (UNIFESP)
    Objective: the aim of this study was to analyze the macroscopic and histological changes that occur in experimental endometriosis after treatment with Uncaria tomentosa.Study design: Experimental endometriosis was induced in twenty-five female Wistar rats. After three weeks, 24 animals developed grade III experimental endometriosis and were divided into two groups. Group U received U. tomentosa extract orally (32 mg/day), and group C (control group) received a 0.9% sodium chloride solution orally (1 ml/100 g of body weight/day). Both groups were treated with gavage for 14 days. At the surgical intervention and after the animal was euthanized, the implant volume was calculated with the following formula: [4 pi (length/2) x (width/2) x (height/2)/3]. the auto-transplants were removed, dyed with hematoxylin-eosin, and analyzed by light microscopy. the Mann-Whitney test was used for the independent samples, and the Wilcoxon test analyzed the related samples, with a significance level of 5%.Results: the difference between the initial average volumes of the autotransplants was not significant between the groups (p = 0.18). However, the final average volumes were significantly different between the groups (p = 0.001). There was a significant increase (p = 0.01) between the initial and final average volumes in the control group, and treatment with the U. tomentosa caused a marked reduction in the growth over time (p = 0.009). Histologically, in the experimental group (n = 10) six rats had a well-preserved epithelial layer, three had mildly preserved epithelium, and one had poorly preserved epithelium. the epithelial layer occasionally presented sporadic epithelial cells. the control group (n = 12) presented seven cases (58.3%) of well-preserved epithelial cells and five cases (41.7%) of mildly preserved epithelial cells.Conclusions: Cat's claw extract appears to be a promising alternative for treating endometriosis. (c) 2010 Elsevier Ireland Ltd. All rights reserved.