Navegando por Palavras-chave "Endothelium"

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    Analysis of low-energy and high-frequency femtosecond laser for the construction of deep anterior donor corneal lamellae
    (Sociedade Brasileira de Oftalmologia, 2014-04-01) Victor, Gustavo; Nosé, Walton [UNIFESP]; Faria e Sousa, Sidney Julio de; Pineda, Roberto; Alves, Milton Ruiz; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Harvard Medical School Department of Ophthalmology
    Purpose: To evaluate the efficacy and reliability of a low-energy femtosecond laser with a high repetition rate for construction of deep anterior donor corneal lamellae. Methods: This was a prospective laboratory investigation. Twenty-five human corneal buttons were femtosecond laser cut to create thick anterior lamellae (diameter, 10mm; thickness, 500µm). The laser cuts were made using an LDV® femtosecond laser in a Ziemer® anterior chamber. To obtain a better edge, the lamellae were trephined with an 8mm trephine (Katena®). The central corneal thickness and the anterior lamellae were measured using a Mitutoyo® thickness gauge with an accuracy of 0.001mm. Results: The central thickness of the 25 corneas ranged from 500 to 705µm (mean, 584 ± 51µm). The thickness of the anterior lamellae ranged from 420 to 480µm (mean, 455 ± 12.7µm). The anterior lamellae diameters were 7.90 ± 0.1mm, and all laser cuts were round. The lamellar interfaces appeared regular by surgical microscopy. There were no cases of inter-lamellar adhesion. Conclusion: The LDV® femtosecond laser appears to be a safe and reliable instrument for cutting deep anterior lamellae from donor corneoscleral buttons. Minimal variation in donor lamellar depth with the laser will be useful for creating donor corneal tissue for deeper anterior lamellar keratoplasty or endothelial keratoplasty surgery or both from a single donor cornea.
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    Avaliação de fatores de risco tradicionais e não tradicionais para doença aterosclerótica em pacientes com síndrome anrifosfolipide primária
    (Universidade Federal de São Paulo (UNIFESP), 2007) Souza, Alexandre Wagner Silva de [UNIFESP]; Sato, Emília Inoue [UNIFESP]
    Objetivo: avaliar o perfil de fatores de risco tradicionais para doença arterial coronariana, níveis de homocisteína, endotelina-1 e de anti-LDL oxidada, e anticorpos anti-lipase lipoprotéica em pacientes com síndrome antifosfolípide (SAF) primária e em controles. Avaliar possível associação entre os diferentes fatores de risco e envolvimento arterial na SAF primária. Métodos: trinta e oito pacientes do sexo feminino com SAF primária e 30 controles foram submetidas à avaliação clínica e laboratorial. Resultados: Não houve diferença significante na freqüência de fatores de risco tradicionais para doença arterial coronariana e escore de risco de Framingham entre pacientes e controles. Porém, as pacientes apresentaram média de fatores de risco para doença coronariana, níveis de LDL e de triglicérides significativamente maiores e níveis de HDL significativamente menores de HDL em relação a controles. Anticorpos anti-lipase lipoprotéica não foram detectados em nenhum dos grupos. Níveis de anti-LDL oxidada, homocisteína e de endotelina-1 foram semelhantes em ambos os grupos, mas, apenas pacientes com síndrome antifosfolípide apresentaram níveis anormais de homocisteína (p=0,031). Hipertensão arterial e a presença de pelo menos um fator de risco para doença coronariana foram mais freqüentes em pacientes com manifestações arteriais. Níveis de homocisteína foram significativamente maiores em pacientes com manifestações arteriais em relação a controles (p=0,036). Análise multivariada demonstrou que hipertensão arterial é um fator de risco independente para envolvimento arterial na SAF. Pacientes com SAF e HAS apresentaram risco 14,8 vezes maior de trombose arterial (IC 95 por cento de 2,1 a 100,0), em comparação aquelas sem HAS (p=0,006). Conclusões: o perfil lipídico das pacientes foi pior que de controles, mas, esta alteração não foi devida a anticorpos anti-lipase lipoprotéica. Foi observada associação entre presença de algum fator de risco tradicional para doença coronariana, hipertensão arterial e maiores níveis de homocisteína com eventos arteriais em pacientes com SAF primária. A hipertensão arterial foi o fator de risco mais importante para o desenvolvimento de trombose arterial em pacientes com SAF primária.
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    Avaliação dos efeitos do flavonoide luteolina sobre a sinalização redox endotelial
    (Universidade Federal de São Paulo, 2023-06-12) Costa, Milena Santana [UNIFESP]; Fernandes, Liliam [UNIFESP]; http://lattes.cnpq.br/2515147908021339; http://lattes.cnpq.br/9438972754416197
    As células endoteliais têm funções metabólicas e sintéticas, apresentando ações autócrinas, endócrinas e parácrinas. O endotélio produz substâncias vasoativas, mediante estímulos humorais e mecânicos, como o óxido nítrico (NO). Espécies reativas de oxigênio (ROS), também produzidas pelo endotélio, podem interromper o equilíbrio do NO e danificar o endotélio. O ânion superóxido (•O2-) reage de forma direta com o NO, levando à redução de sua biodisponibilidade e originando o peroxinitrito (ONOO–), por meio do qual resíduos de tirosina de proteínas essenciais para a saúde vascular podem ser nitrados. Dessa forma, entende-se que o desbalanço das reações de redução/oxidação (redox) esteja diretamente envolvido no desenvolvimento da disfunção endotelial e no aparecimento de doenças vasculares. A enzima superóxido dismutase (SOD) é a primeira linha de defesa contra o estresse oxidativo, produzindo peróxido de hidrogênio (H2O2) e oxigênio (O2) a partir do •O2-. A luteolina é um flavonoide, podendo ser encontrada em flores, ervas, vegetais e especiarias. A luteolina apresenta, em concentrações micromolares, ação antioxidante ou pró-oxidante. O trabalho teve como objetivo investigar os efeitos causados pela luteolina em células endoteliais de veia umbilical humana (HUVEC), utilizando-se concentrações de 20 e 100 μM do flavonoide com período de incubação de 1 hora, podendo então, esclarecer seu papel na sinalização redox endotelial e no tratamento e/ou prevenção de doenças vasculares. Para isso, foram avaliadas a nitração de resíduos de tirosina e a expressão de SOD 1 e SOD 2 por imunocitoquímica de fluorescência, e a atividade da SOD por ensaio colorimétrico. Após as incubações, foi possível observar a diminuição da nitração de tirosina e da expressão de SOD 2, assim como um aumento na expressão de SOD 1, sem alterações significativas na atividade total da enzima. Conclui-se que o endotélio possui papel fundamental na fisiologia vascular, no entanto os efeitos diretos da luteolina sobre ele não são extensamente conhecidos. O projeto colaborou em demonstrar que a luteolina pode contribuir com a saúde vascular, sugerindo sua futura aplicação no cuidado de transtornos circulatórios e instigando uma maior atenção ao seu efeito antioxidante no endotélio, o qual deve ser avaliado para melhor compreensão.
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    Células endoteliais circulantes em diferentes tipos de tratamentos da Anemia Falciforme
    (Universidade Federal de São Paulo (UNIFESP), 2020-12-15) Souza, Claudia Regina Lustosa [UNIFESP]; Figueiredo, Maria Stella [UNIFESP]; Universidade Federal de São Paulo
    Sickle cell anemia (SCA) is characterized by anemia and pain due to chronic hemolysis and recurrent episodes of ischemia, respectively. In the presence of hypoxia, the anomalous hemoglobin (Hb), HbS, triggers microvascular occlusion, a phenomenon enhanced by the adhesion of erythrocytes and leukocytes to the vascular endothelium. As a result, vasculopathy and inflammation characteristic of SCA are observed, with the endothelial cell playing an important role. The endothelium is a dynamic, balanced tissue, where the circulation compartment reflects both the lesion and the regeneration of the vascular tree. In this compartment, circulating endothelial cells (CECs) released from vascular injury and progenitor endothelial cells (PECs) involved in vascular regeneration are identified. However, the importance of these cells in SCA and their relationship with the type of treatment still needs further clarification. Objectives: To evaluate the endothelial phenotype of individuals with sickle cell anemia and determine whether the type of treatment influences this manifestation. Casuistic: 45 patients with SCA were analyzed, followed up at the Hemoglobinopathies Outpatient Clinic of Escola Paulista de Medicina (EPM / UNIFESP). According to the treatment, the following groups were distributed: a) SS (N = 11) - steady state without hydroxyurea (HU); b) SS-HU (N = 19) – in use of maximum tolerated dose of HU; c) SS-TF (N = 15) - chronic transfusion regimen. Control group (CN, N = 13) consisting of healthy individuals. Work approved by Ethical Comittee and participants signed an informed consent form. Exclusion criteria: pregnant and puerperal women, vaso-occlusive crisis in the last 3 months, chronic renal failure and active leg ulcer. In the SS and SS-HU groups, those who received red blood cell transfusion in the last 3 months were also excluded. Methods: Laboratory evaluation: complete blood count, hemolysis tests and C-reactive protein (CRP). Identification and quantification of CECs and PECs performed by Flow Cytometry (FACSCantoII®). Results: The SS group had a higher number of CECs (median: 2.73; range: 0 - 20.0) than the CN (0.39; 0 - 4.20) and the SS-TF (0.67 ; 0 - 2.99), p = 0.016 and 0.013 respectively. The SS-HU group showed higher PECs quantification (0; 0 - 6.62) than the SS and SS-TF groups (0; 0 - 0 in both groups), p <0.05. There was no relationship between determination of CECs and clinical manifestations, however patients with priapism showed higher values (2.28; 0.74 - 6.09) than those without this manifestation (0.68; 0 - 20.0) with p = 0.055. There was no significant correlation between PECs data and clinical manifestations. Discussion & Conclusion: Our results corroborate previous studies that associate the increase in CECs with endothelial complications of SCA. The presence of fewer PECs in groups SS and SS-TF when compared to SS-HU group suggests that HU can provide endothelial protection and raises the question of its indication for all SCA patients in order to prevent serious manifestations related to endothelial dysfunction.
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    Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
    (Consel brasil oftalmologia, 2016) Vianna, Lucas Monferrari Monteiro [UNIFESP; Li, Hao-Dong; Holiman, Jeffrey D.; Stoeger, Christopher; Belfort, Rubens Junior [UNIFESP]; Jun, Albert S.
    Purpose: To compare cryopreserved human corneal endothelial cells (HCECs) grown in human serum-supplemented media (HS-SM) with cryopreserved HCECs grown in fetal bovine serum-supplemented media (FBS-SM). Methods: Three pairs of human corneas from donors aged 8, 28, and 31 years were obtained from the eye bank. From each pair, one cornea was used to start a HCEC culture using HS-SM
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    Disfunção endometrial tardia apos revascularização percutanea: resposta coronária a acetilcolina antes e depois da aplicação de nitroglicerina transdermica
    (Universidade Federal de São Paulo (UNIFESP), 1996) Lima, Valter Correia de [UNIFESP]; Carvalho, Antonio Carlos [UNIFESP]
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    Disfunção endotelial e estado nutricional relativo ao selênio em pacientes pediátricos durante a fase aguda da resposta inflamatória sistêmica
    (Universidade Federal de São Paulo (UNIFESP), 2021) Lopes Junior, Emilio [UNIFESP]; Leite, Heitor Pons [UNIFESP]; Universidade Federal de São Paulo
    The association between endothelial adhesion molecules and selenium has been shown experimentally, but clinical studies have not clarified whether selenium status has an active role in endothelial function. The objectives of this study were to investigate whether there is an association between erythrocyte selenium and (a) the magnitude of endothelial activation and (b) the severity of multiple organ dysfunction in children during the Systemic Inflammatory Response Syndrome (SIRS). A prospective cohort study was carried out in 109 children with SIRS admitted to a pediatric ICU. Erythrocyte and plasma selenium were measured on admission and Selenoprotein P and soluble plasma forms of Intercellular Adhesion Molecules 1 (sICAM-1), Vascular Cell Adhesion Molecule 1 (sVCAM-1), sPselectin and EndoCAM on days 1, 2 and 3. ANOVA for repeated measures were adjusted for clinical severity paremeters, C-reactive protein, procalcitonin and serum lactate. The effect of selenium on organ dysfunction was defined by the Pediatic Logistic Organic Dysfunction (PELOD-2) during ICU stay. Erythrocyte selenium was associated with sPselectin and EndoCAM: an increase of 10 μmol/dL resulted in increases of 43.2 ng/mL (95% CI: 17.2; 69.2, p = 0.001) in sP-selectin and of 0.04 ng/mL (95% CI: 0.02; 0.06, p <0.001) in EndoCAM. Erythrocyte selenium was associated with a decrease in PELOD-2 (β: -0.3; 95% CI: -0.5; -0.06, p=0.015). Plasma selenium was not related to any of the outcomes. Erythrocyte selenium is associated with endothelial activation that occurs in children in the early phase of SIRS and has a possible protective effect on organ dysfunction.
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    Disfunção endotelial venosa em pacientes com doença de Chagas sem insuficiência cardíaca
    (Sociedade Brasileira de Cardiologia - SBC, 2006-06-01) Plentz, Rodrigo Della Méa [UNIFESP]; Irigoyen, Maria Claudia [UNIFESP]; Muller, Andreia Simone [UNIFESP]; Casarini, Dulce Elena [UNIFESP]; Rubira, Marcelo Custodio; Moreno Junior, Heitor; Mady, Charles; Ianni, Bárbara Maria; Krieger, Eduardo Moacir; Consolim-Colombo, Fernanda; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP); Universidade Estadual de Campinas (UNICAMP); Universidade de Cruz Alta
    OBJECTIVE: To analyze the venous endothelial function in Chagas' disease patients without heart failure. METHODS: The Chagas' disease Group (G1) was composed by 14 women and 2 men aged 46 ± 2,7 and the Control Group (G0) by 7 women and 1 man matched by age, weight and height. Dorsal Hand Vein Compliance Technique was used to evaluate the venous endothelial function. Crescent doses of phenylephrine were infused to get a 70% pre-constriction of the vein; after that, acetylcholine and sodium nitroprusside were respectively administrated to analyze the endothelium-dependent and -independent venodilation. RESULTS: No significant systemic hemodynamic changes were observed in both groups during the experiment. The necessary phenylephrine dose to reach 70% pre-constriction of the vein was significantly higher in the G1 (1116 ± 668,2 ng/ml) compared to G0 (103 ± 28 ng/ml) p = 0,05. The endothelium-dependent venous dilation was significantly lower in G1 (65,5 ± 8%) compared to G0 (137 ± 20 %) p = 0,009. No difference was observed in the endothelium-independent venous dilatation between groups. CONCLUSION: Patients with Chagas' disease without heart failure presented venous endothelial dysfunction.
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    Efeitos bioquímicos e imunológicos da terapia anti-hipertensiva
    (Universidade Federal de São Paulo (UNIFESP), 2009) Brandão, Sergio Augusto Bueno [UNIFESP]; Fonseca, Francisco Antonio Helfenstein [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objetivos: Lipoproteínas oxidadas e anticorpos (Abs) anti-LDL oxidada (antiLDLox) foram detectados no plasma em resposta à elevação da pressão arterial, sugerindo a participação do sistema imune adaptativo. Postulamos que o tratamento da hipertensão possa modificar a resposta imune diminuindo o estímulo oxidativo. Analisamos os títulos de Abs anti-LDLox após adequado controle da hipertensão arterial. Métodos: Títulos de Abs anti-LDLox foram examinados em pacientes com hipertensão arterial no estágio 1 (n=94), sem manifestação clínica prévia de aterosclerose ou fatores de risco clássicos para a doença coronariana e isentos de medicações anti-hipertensivas. Pacientes foram distribuídos aleatoriamente para terapia com perindopril, hidroclorotiazida ou indapamida por 12 semanas, com adição de perindopril, se necessário para adequado controle pressórico. Títulos de Abs anti-LDLox foram mensurados por ELISA. Resultados: O tratamento anti-hipertensivo reduziu as pressões arteriais casuais e as obtidas pela MAPA (p<0,0005), diminuiu os níveis plasmáticos de substâncias reativas ao ácido tiobarbitúrico (p<0,05) e aumentou os títulos dos Ab anti-LDLox (p<0,005) e a dilatação mediada pelo fluxo (p<0,0005), independentemente do tratamento instituído. Conclusões: Aumento nos títulos de Abs anti-LDLox após terapia anti-hipertensiva ao lado de melhor função endotelial e diminuição do estresse oxidativo sugerem que a despeito de maior estímulo oxidativo estar presente na hipertensão, os Abs anti-LDLox podem ser potenciais biomarcadores protetores para monitorizar o tratamento da hipertensão.
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    Endotélio venoso: estabelecimento e caracterização de culturas primárias e a produção de fatores derivados do endotélio de veia cava e veia porta de ratos
    (Universidade Federal de São Paulo, 2017-03-17) Trindade, Marcio Renato [UNIFESP]; Fernandes, Liliam [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The endothelial cell plays an essential role in vascular control by the release of Endothelium- Derived Relaxing Factors (EDRFs) [nitric oxide (NO), prostacyclin (PGI2) and endotheliumderived hyperpolarizing factor (EDHF)], and Endothelium-Derived Constrictor Factors (EDCFs) [prostaglandin H2 (PGH2) and Thromboxane A2 (TXA2), reactive oxygen species, Angiotensin II (Ang II) and Endothelin-1 (ET-1)]. Most of the studies related to EDRFs and EDCFs refers to arterial territories and little is known about distinct venous territories, such as vena cava (VC) and portal vein (PV). The present study investigated the physiology of the venous endothelial cell using primary cultures of VC and PV of rats. Venous segments were sectioned longitudinally, plated with the endothelial side facing down, covered with culture medium and kept in a 5% CO2 incubator at 37°C for 5 days. After removal of the explants, the cells were subcultured and cultures were studied between 4th and 5th passages. The characterization of the cultures was performed by immunocytochemistry for the specific markers von Willebrand Factor (vWF) and endothelial NO synthase (eNOS), and positive staining was also observed for ET-1 receptor (ETB) and Ang II receptors (AT1 and AT2). Basal NO production was observed in endothelial cultures pre-incubated with the DAF-2DA fluorescent probe and observed in confocal microscopy, and complemented by eNOS expression analysis determined by western blot (n = 4). Prostanoids production was quantified in the culture supernatants by enzyme-immunoassay technique (n = 5 to 6). Endothelial cultures of VC and PV showed similar values in the baseline rates of PGH2 (0.75 ± 0.04 ng/mL vs 0.76 ± 0.02 ng / mL, respectively); these levels were increased after stimulation with Ang II [1μM] (1.00 ± 0.07 * ng/mL for VC vs 1.13 ± 0.07 * ng/mL for PV). Baseline levels of PGI2 were different between cultures, with values of 29.12 ± 1.24 ng/mL for VC vs 35.10 ± 1.27 * ng/mL for PV. Ang II stimulation [1μM] increased PGI2 production in VC cells (36.50 ± 1.89 * ng/mL) but not in PV (37.70 ± 1.38 ng/mL) (* P <.05); however, COX expression (1 and 2) was similar between cultures (determined by western blot, n = 7). Stimulation with ET-1 [1μM] did not modify the release of prostanoids in both cultures. In summary, the venous endothelium can be isolated and studied through primary cultures; these cells, when studied in vitro, produce detectable amounts of NO and prostaglandins. The participation of PGI2 in the venodilation can be pronounced in PV when compared to VC. The consistent increase in PHG2 production by Ang II in VC and PV endothelium indicates the important endothelial modulation in the venoconstriction. The present data reveal new aspects of the vascular physiology, and may contribute to a better understanding of the venous responses to the blocking agents of the Renin Angiotensin System.
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    Estresse oxidativo, medida do complexo médio intimal e reatividade endotelial em crianças com níveis aumentados de homocisteína
    (Universidade Federal de São Paulo (UNIFESP), 2011) Souza, Fabíola Isabel Suano de [UNIFESP]; D'Almeida, Vânia [UNIFESP]
    Objetivo: Avaliar em crianças pré-púberes, com concentrações aumentadas de homocisteína (Hcy), a presença de fatores de risco para doença cardiovascular (DCV) e relacioná-los com a reatividade endotelial e espessura médio intimal. Métodos: Em estudo transversal, 35 crianças com Hcy≥7,3 μmol/L foram pareadas, por sexo e idade, com 29 crianças com Hcy≤4,1 μmol/L. Dados obtidos: peso e estatura; circunferência abdominal e pressão arterial sistêmica (PA). Dosagem do perfil lipídico, glicemia, vitaminas (B12, B6 e ácido fólico) e marcadores de estresse oxidativo (relação glutationa oxidada/reduzida, substâncias reativas ao ácido tiobarbitúrico e peróxidos lipídicos). Foi realizada ultrassonografia Doppler para avaliação da medida do complexo médio-intimal das carótidas comuns (CMI) e vasodilatação endotélio-induzida da artéria braquial (VEI). Análise estatística: utilizou-se o teste t-Student, Mann-Whitney e Qui-quadrado. Resultados: Não se observou diferença entre os grupos em relação à idade (média 8,6±1,4 anos), gênero e renda per capita. Não se observou diferença estatisticamente significante entre os grupos em relação ao perfil lipídico, glicemia, PA e marcadores de estresse oxidativo. Crianças com Hcy ≥7,3 μmol/L tiveram maior prevalência de excesso de peso (37,1% vs 13,8%; p=0,035) e menores concentrações plasmáticas de vitamina B12 (597,7±302,4 pg/mL vs 822,4±332,5 pg/mL; p=0,007). Após ajuste para condição nutricional, não se observou diferença em relação à medida do CMI e VEI da artéria braquial em crianças com valores maiores e menores de Hcy. Conclusões: No presente estudo apenas o excesso de peso associou-se com concentrações aumentadas de Hcy (≥ 7,3 μmol/L). Esses dados sugerem que o envolvimento da Hcy no risco cardiovascular pode não ser direto e sim, combinado a outros fatores.
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    Estudo duplo-cego, placebo-controlado, do uso da fluvastatina sobre a pressão arterial, a sensibilidade à insulina e a morfologia cardiovascular em pacientes portadores de hipertensão arterial
    (Universidade Federal de São Paulo (UNIFESP), 2009-01-27) Teixeira, Andrei Alkmim [UNIFESP]; Tavares, Agostinho [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Among hypertensive patients, cardiovascular disease morbidity is common, even in those who are adequately treated. New pharmacologic strategies to mitigate the burden of arterial hypertension are needed. This 12-month, randomized, double-blind placebocontrolled study investigated the effect of statin (fluvastatin) treatment on ambulatory blood pressure (ABP), exercise blood pressure (EBP), myocardial structure, endothelial function and insulin resistance in 50 hypertensive patients. At baseline, the groups were comparable in terms of demographic characteristics, ABP, EBP, endothelial function and homeostasis model assessment of insulin resistance (HOMAIR). At the end of the study, there was no difference between groups in terms of resting systolic blood pressure. However, maximum systolic EBP was lower in the treatment group than in the placebo group (175 ± 18 mmHg vs. 192 ± 23 mmHg, P < 0.05), as was left ventricular mass index (LVMI; 82 ± 15 vs. 100 ± 23, P < 0.05) and HOMA-IR index was lower after fluvastatin treatment (2.77 ± 1.46 vs. 3.33 ± 1.73, P < 0.05). Changes in lipid profile were not correlated with blood pressure, endothelial function, LVMI, or HOMA-IR data. In hypertensive patients, fluvastatin can improve maximum systolic EBP, myocardial remodeling and insulin resistance, independently on lipid profile variations and endothelial function.
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    Estudo morfométrico do efeito do Tenoxicam e do seu diluente no endotélio venoso, em coelhos
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2001-09-01) Schnaider, Taylor Brandão; Andrade, Carlos Henrique Vianna De; Juliano, Yara [UNIFESP]; Novo, Neil Ferreira [UNIFESP]; Engelman, Miriam De Fátima Brasil; Schnaider, Gabrielle Sormanti; Schnaider, Caroline Sormanti; UNIPA Faculdade de Medicina de Pouso Alegre Departamento de Clínica Médica; UNIPA Faculdade de Medicina de Pouso Alegre; Universidade Federal de São Paulo (UNIFESP); UNIPA Faculdade de Medicina de Pouso Alegre Departamento de Ciências Fisiomorfológicas e Patologia
    To evaluate, by the morphometry, the effect of tenoxicam and its diluent in the venous endothelium, were used 48 white male rabbits (Oryctolagus cuniculus), of the New Zealand branch, with age over 10 weeks and weight varying between 2.350 and 3.500 grams. The rabbits were divided in two groups: Experiment and Control, and analyses were conduced in 6, 12 and 24 hours after the procedure. Into the right and left mediales auriculares veins of the Experiment group was injected tenoxicam or tenoxicam and its diluent; in the Control group 0,9% sodium chloride was injected. No statistically significant difference was observed between the animals weight in both groups before the performance of the procedure. It was possible to observe, after the use of tenoxicam with its diluent or the isolated diluent, that the nuclei diameter of the cells of the venous endothelium showed a dimension, markedly smaller when compared to the Control Group, in which was injected 0,9% sodium chloride. The results found allow to conclude that tenoxicam with its commercial or its isolated diluent reduce the nuclei diameter of the cells of the venous endothelium, where they were injected.
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    Heparina: interação com a matriz células endoteliais e atividade antitrombótica
    (Universidade Federal de São Paulo (UNIFESP), 2000) Trindade, Edvaldo da Silva [UNIFESP]; Nader, Helena Bonciani [UNIFESP]
    Heparina e drogas antitromboticas em geral estimulam, de forma especifica, a sintese do proteoglicano de heparam sulfato (PGHS) em celulas endoteliais. O efeito e composto e celula especificos. Ensaios cineticos com heparina mo que o estimulo e tempo e dose dependentes, e que esta se liga na superficie celular, sugerindo a existencia de possiveis receptores. Pelo presente trabalho, desenvolveu-se uma heparina marcada com biotina com o intuito de estudar a interacao com celulas endoteliais. A reacao de biotinilacao resultou num composto contendo uma biotina para cada residuo de acido urom'co. Essa heparina apresentou menor atividade anticoagulante (38U.I./mg), desprezivel atividade hemorragica, maior peso molecular (l8,6kDa), quando comparados com a heparina padrao, que possui 166U.I./mg, potente acao hemorragica e peso molecular de l3kDa. Essa heparina ainda, deixou de ser substrato para a heparinase e heparitinase H, porem manteve a capacidade de interacao com a Antitrombina III e a Fibronectina Os dados mostraram tambem que esta heparina e capaz de estimular a sintese de PGHS, como a heparina padrao. O(s) sitio(s) de ligacao para a heparina, nas celulas endoteliais, foram investigados utilizando-se como modelo, a heparina biotinilada em tecnicas de deteccao citoquimica e analise em microscopia confocal. Alem desta heparina, foram utilizados lectina WGA, que interage com a superficie celular e anticorpos especificos tanto para a superficie celular (anti-sindecam 4), como para a matriz extracelular (anti-fibronectina). Esses compostos foram ensaiados empregando-se tanto celulas recem-plaqueadas, sub-confluentes e confluentes, como em suspensao. Ainda a matriz extracelular livre de celulas foi investigada. Todos estes experimentos, revelaram que a ligacao da heparina biotinilada ocorreu somente nos componentes da matriz extracelular. Experimentos mantendo-se as celulas em presenca de heparina biotinilada por 22 horas, mostraram que ocorre o processo de internalizacao da mesma. Este conjunto de resultados sugere que o estimulo da sintese de PGHS, causado pela heparina possa ser independente da sua interacao com a superficie celular
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    Impacto dos stents e do sirolimus por via oral na vasomotricidade coronariana dependente e independente do endotélio
    (Universidade Federal de São Paulo (UNIFESP), 2012-05-30) Fernandes, Rósley Weber Alvarenga [UNIFESP]; Lima, Valter Correia de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Rationale: There is no consensus regarding the impact of stenting on long-term endothelial function. There have been reports of increased endothelial dysfunction with Sirolimus-eluting stents as compared to bare metal stenting (SC). Objectives: This study aims to assess the impact of SC and the effect of oral Sirolimus on endothelial function. Methods: Forty-five patients were randomized into three groups: SC + high-dose oral Sirolimus (initial dose of 15 mg, followed by 6 mg/day during four weeks); SC + lowdose Sirolimus (6 mg followed by 2 mg daily during four weeks); and SC without Sirolimus. Changes in vasoconstriction or vasodilation in a 15 mm segment starting at the distal stent end in response to acetylcholine and nitroglycerin were assessed by quantitative angiography. Results: The groups had similar angiographic characteristics. The percent variation in diameter in response to acetylcholine was similar in all groups at the two time points (p = 0.469). Four hours after stenting, the target segment presented an endothelial dysfunction that was maintained after eight months in all groups. In all groups, endothelium-independent vasomotion in response to nitroglycerin was similar at four hours and eight months, with increased target segment diameter after nitroglycerin infusion (p = 0.001). Conclusions: The endothelial dysfunction was similarly present at the 15 mm segment distal to the treated segment, at 4 hours and 8 months after stenting. Sirolimus administered orally during 4 weeks to prevent restenosis did not affect the status of endothelium-dependent and independent vasomotion.
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    Investigação do processamento das proteínas do sistema calicreína-cinina plasmático na interação com células endoteliais
    (Universidade Federal de São Paulo (UNIFESP), 2019-02-28) Assis, Mirian Coelho de [UNIFESP]; Motta, Guacyara da [UNIFESP]; Oliveira, Cleide de [UNIFESP]; http://lattes.cnpq.br/4060773569354807; http://lattes.cnpq.br/0050968690289066; http://lattes.cnpq.br/7024315470446395; Universidade Federal de São Paulo (UNIFESP)
    Endothelial cells (ECs) correspond to the coating of both vessels the lymphatic vessels and constitute the inner layer of these glasses These cells are flattened epithelial and distributed in monolayer presenting great heterogeneity, since they differ in size, length and height, shape and number of stored vesicles, and this is related to the caliber of the blood vessel or a tissue (Aird, 2007; Dela-Paz et al., 2009). The endothelium is considered an endocrine organ capable of producing and secreting a variety of chemicals that control clotting, tonus and plasma levels of endogenous mediators and lipoproteins (Carvalho et al., 2005). However, it is also involved in a number of pathological conditions, such as atherosclerosis and cancer (Dela-Paz et al., 2009), and in diffuse intravascular coagulation the endothelium is the interface between inflammation and inappropriate activation of the coagulation system (Kato, 2014). The endothelium exerts several functions as regulation of the inflammatory response and inhibition of cell proliferation and migration. The release of substances such as vasodilators and vasoconstrictors conserve laminar blood flow, preserving the fluidity of the plasma membrane, creating mechanisms anticoagulants. Vasodilators such as nitric oxide, bradykinin and adenosine, in channels of K + dependent on ATP, its activation by vasodilators, associated with hyperpolarization of the membrane, the closure of the K + , act on the cells of the smooth muscle cells present in the tunica media of arterioles resulting in reduced blood flow resistance and decreased blood pressure. On the other hand vasoconstrictors include endothelin and angiotensin II which promote smooth muscle contraction and increase the (Baxter et al., 2006), and the effect of the antihypertensive effect on the blood pressure (Baxter et al. The functions exerted by the endothelium regulate cell-cell interactions and cell-matrix, the expression and presence of membrane-bound receptors being associated with numerous molecules including proteins, carrier particles of lipids, metabolites and hormones, and glycosaminoglycan sugars (Rajendran et al., 2013, Muller, 2013).
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    Lack of Association of Homocysteine Concentrations with Oxidative Stress, Alterations in Carotid Intima Media Thickness and Endothelial Reactivity in Prepubertal Children
    (Karger, 2013-01-01) Suano de Souza, Fabiola Isabel [UNIFESP]; D'Almeida, Vania [UNIFESP]; Affonso Fonseca, Fernando Luiz [UNIFESP]; Hix, Sonia [UNIFESP]; Miranda, Robson [UNIFESP]; Torres Rossi, Rosana Gomes de [UNIFESP]; Ribeiro, Renata [UNIFESP]; Sarni, Roseli Oselka Saccardo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Background and Aims: Our purpose was to assess the presence of risk factors for cardiovascular diseases (alterations in the lipid profile, fasting glycaemia, high arterial pressure values, oxidative stress, increased intima media thickness and impaired endothelium-dependent vasodilation) in prepubertal children with elevated homocysteine (Hcy) levels. Methods: in a cross-sectional study, 35 children with Hcy levels >= 7.3 mu mol/l were matched by gender and age with 29 children with Hcy levels mu mol/l. Our collected data included weight, height, waist circumference, systemic arterial pressure, lipid profile, fasting glycaemia and oxidative stress markers. A Doppler ultrasound was performed to measure the carotid intima media thickness and the endothelium-dependent vasodilation of the brachial artery. Results: There were no statistically significant differences between the groups regarding the lipid profile and fasting glycaemia, blood pressure or oxidative stress. Overweight and obesity (body mass index z-score > +1) were more frequent in the group with Hcy mu mol/l when compared to the group with Hcy mu mol/l [13/35 (37.1%) vs. 4/29 (13.8%); p = 0.035]. After adjusting for nutritional status, there were no differences in the intima media thickness and endothelium-dependent vasodilation between children with high and low Hcy levels. Conclusions: Among the cardiovascular risk factors investigated in the present study, which was based on prepubertal children, only overweight and obesity were associated with increased Hcy concentrations (>= 7.3 mu mol/l). Copyright (C) 2013 S. Karger AG, Basel
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    Microdensidade vascular no leito placentário em pacientes portadoras de Pré-eclâmpsia: estudo transversal descritivo comparativo
    (Universidade Federal de São Paulo (UNIFESP), 2006-03-29) Coelho, Tarcisio Mota [UNIFESP]; Sass, Nelson [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    CONTEXT AND OBJECTIVE Morphological changes in the spiral arteries of the placental bed have been studied in patients with preeclampsia one of de most cause of maternal and perinatal morbidly and mortality. According to the reports, vasospasm and vascular endothelial injury are two major pathological conditions of preeclampsia. OBJECTIVE: The aim of the study was to investigate microvessel density of spiral arteries in the placental bed in pregnancies complicated by hypertension and proteinuria as well as in normal pregnancy. DESIGN AND SETTING: A cross-sectional survey of imunohistochemical study using CD 34, of placental bed biopsies containing spiral arteries based on women undergoing caesarean sections for clinical and obstetrical reasons performed at the Universidade Federal de São Paulo, São Paulo, Brazil. METHODS: Placental bed biopsies were obtained during caesarean section after placental removal, under direct visualization of central area of placental insertion and measurement of microvessel density spiral arteries by immunohistochemical in decidual and miometrial segments, using antibody CD34. RESULTS: Biopsies containing spiral arteries were obtained from 34 hypertensive pregnant women with proteinuria, and 26 normotensive pregnant women. Microvessel density in decidual and miometrial segments of placental bed was compared in both groups. It was observed that, with the increase of blood pressure and proteinuria, microvessel density dropped gradually. CONCLUSION: The presence and elevations of high levels of hypertension and proteinuria may
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    Acesso aberto (Open Access)
    Microvessel density in the placental bed among preeclampsia patients
    (Associação Paulista de Medicina - APM, 2006-01-01) Coelho, Tarcisio Mota [UNIFESP]; Sass, Nelson [UNIFESP]; Camano, Luiz [UNIFESP]; Moron, Antonio Fernandes [UNIFESP]; Mattar, Rosiane [UNIFESP]; Stávale, João Norberto [UNIFESP]; Silva, Maria Regina Régis [UNIFESP]; Martins, Marília da Glória; Nogueira Neto, João; Universidade Federal de São Paulo (UNIFESP); Universidade Federal do Maranhão Medicine III Department
    CONTEXT AND OBJECTIVE: Morphological changes in the spiral arteries of the placental bed have been studied in patients with preeclampsia, one of the largest causes of maternal and perinatal morbidity and mortality. The reports show that vasospasm and vascular endothelial injury were two major pathological conditions for preeclampsia. The aim of this study was to investigate the microvessel density of spiral arteries in the placental bed, in pregnancies complicated by hypertension and proteinuria, and in normal pregnancies. DESIGN AND SETTING: This was a cross-sectional survey of immunohistochemical studies on biopsies from the spiral arteries of the placental bed, among women undergoing cesarean sections for clinical and obstetrical reasons at Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil. METHODS: Placental bed biopsies were obtained during cesarean section after placenta removal, with direct viewing of the central area of placenta insertion. The microvessel density of spiral arteries was measured by immunohistochemical methods in decidual and myometrial segments, using CD34 antibody. RESULTS: Biopsies containing spiral arteries were obtained from 34 hypertensive pregnant women with proteinuria, and 26 normotensive pregnant women. The microvessel densities in decidual and myometrial segments of the placental bed were compared between the groups. It was observed that, with increasing blood pressure and proteinuria, the microvessel density gradually decreased. CONCLUSION: The presence of high levels of hypertension and proteinuria may be associated with a progressive decrease in microvessel density in the placental bed.
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    Neovascularization after surgical exposure of rat external jugular vein endothelium
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-12-01) Nascimento, José Francimário Bezerra Do [UNIFESP]; Pitta, Guilherme Brandão Benjamin; Miranda Junior, Fausto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); University of Health Sciences of Alagoas Department of Surgery
    PURPOSE: To investigate the neovascularization after exposure of the external jugular venous endothelium in an experimental model. METHODS: The sample was composed of 60 male rats of Wistar OUT B breed provided by animal colony of the Medicine College of Juazeiro do Norte, weighing 250 to 350g, aged 90-120 days. Randomized study in OUT B Wistar rats, open, with 60 days duration. The animals were distributed into three groups of 20 specimens and were subjected to the following: Group 1: neck incision with dissection, ligation and section of the external jugular vein. Group 2: neck incision with dissection and ligation of the external jugular vein. Group 3: cervicotomy without dissection of the external jugular vein without ligation or section. The animals were sacrificed, half of them in 30 days and the other half within 60 days. The material in block harvested from the operated site were sectioned and stained for immunohistochemistry with CD34 marker. RESULTS: Neovascularization occurred with level of significance when compared group 1 to group 3 at 30 days (p=0.0076) and the same occurred at 60 days (p=0.0001) (Newman-Keuls test). CONCLUSION: The group with exposure of the venous endothelium showed a significant increase of neovascularization when compared with other groups.