Navegando por Palavras-chave "Endothelin-1"
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- ItemAcesso aberto (Open Access)Caracterização vascular periférica de pacientes portadores de glaucoma primário de ângulo aberto(Universidade Federal de São Paulo (UNIFESP), 2020-12-18) Muller, Elise Vivan Taniguchi [UNIFESP]; Paranhos Junior, Augusto [UNIFESP]; Universidade Federal de São PauloPurpose: To evaluate peripheral microvascular characteristics of primary open-angle glaucoma (POAG) patients and healthy controls, along with endothelin-1 serum concentration and autoantibodies measurements. Patients and Methods: Subjects underwent a visual field test (Humphrey Visual Field Analyzer, Carl Zeiss, Dublin, USA), swept source optical coherence tomography (SS-OCT) (Deep Range Imaging OCT Triton, Topcon, Tokyo, Japan), nailfold capillaroscopy (NFC) (Stereo Microscope SZ40, Olympus, Tokyo, Japan), laser Doppler imaging (LDI) (Moor LDI-VR, Moor Instruments, Axminster, United Kingdom) and venous blood collection. Ophthalmic tests evaluated the presence and the stage of POAG and confirmed the absence of any abnormality among controls. NFC evaluated the presence and the number of microhemorrhages, tortuous capillaries, branched capillaries, dilated capillaries, mega-capillaries and bizarre capillaries, besides the number of capillary loops/mm and the deletion score; LDI measured fingertip blood flow (FBF) in four different timepoints and blood samples were evaluated for determination of serum endothelin-1 concentrations along with serum auto-antibodies measurements. Results: Sixty-eight subjects (43 patients with POAG and 25 healthy controls) were enrolled in the study. 65.1% of POAG patients presented with hemorrhages in the nailfold capillaroscopy versus 25.0% of healthy controls (p=0.003, chi-Square). There was a significant difference in the mean FBF at baseline in POAG patients vs. controls (293.6 ± 100.2 vs. 388.8 ± 52.0 perfusion units [PU]), respectively, p<0.001, mixed models), together with a significant difference in the mean FBF 10 and 20 minutes after cold stimulus in POAG patients in comparison to controls (p<0.001 for all comparisons, mixed models). However, nor the analysis of endothelin-1 concentrations (1.7 ± 1.4 vs. 1.6 ± 1.5 pg/ml, p=0.71, t-test) or the autoantibodies measurements (p>0.05 for all, chi-square) showed any difference between the groups. There was a positive correlation between basal FBF of LDI and retina nerve fiber layer (RNFL) measurements from SS-OCT (r=0.441, p=0.001, Pearson’s correlation coefficient), as well as between basal FBF and the mean deviation index of visual field (r=0.272, p=0.03, Pearson’s correlation coefficient). Conclusions: POAG patients presented significant peripheral microvascular abnormalities compared to healthy controls. This study supports the vascular theory associated to POAG.
- ItemSomente MetadadadosEfeito Do Bloqueio Crônico Do Receptor De Endotelina-1 Sobre Os Principais Fatores Da Doença Renal Crônica(Universidade Federal de São Paulo (UNIFESP), 2018-01-18) Souza Filho, Agnaldo Caires De [UNIFESP]; Borges, Fernanda Teixeira [UNIFESP]Diabetes And Hypertension Are The Major Causes Of Chronic Kidney Disease That Leads To Progressive Loss Of Kidney Function And Culminates With The Final Stage Of Kidney Disease. Macitentan Is A Novel Endothelin 1 Receptor Antagonist, With A 50-Fold Selectivity For The Etb Receptor. The Objective Of This Study Was To Evaluate The Effect Of Macitentan (10 And 25 Mg / Kg) On Shr Animals Where Diabetes Was Induced By Administration Of Streptozotocin. Spontaneously Hypertensive Animals (Shr) Were Divided Into Control (Ctl), Diabetic (Diab), Diabetic Subjects Treated With Macitentan 10 (Mac10) And 25 Mg/Kg (Mac25) For 60 Days. The Plasma Glucose, Weight, Creatinine And Urea Levels, Fraction Of Sodium Excretion And Proteinuria Were Evaluated At The Beginning, 30 And 60 Days After The Induction Of Diabetes. Immunostaining For Endothelin-1, Ngal, Renin, Angiotensin I And Ii Was Performed. Lipid Peroxidation And Excretion Of Peroxides Were Analyzed. Nitrosilative Stress Was Determined By The Production And Urinary Excr
- ItemSomente MetadadadosEndothelial Markers in Schistosomiasis Patients With or Without Portal Hypertension(Springer, 2009-06-01) Toledo, Carlos Fischer de [UNIFESP]; Carvente, Claudia Teresa [UNIFESP]; Shigueoka, David Carlos [UNIFESP]; Borges, Durval Rosa [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Purpose To evaluate the serum levels of von Willebrand factor (vWF), vascular endothelial growth factor (VEGF), endothelin-1 (ET-1), and endothelin-3 (ET-3) in patients with the isolated, inactive form of Schistosomiasis mansoni. Patients were classified into two groups: 10 patients without (SM group) and 18 with (PH group) portal hypertension. Results Serum albumin, VEGF, and vWF levels did not differ between the two groups (P > 0.05). the prothrombin time (INR), number of platelets, spleen size, splenic vein diameter, and endothelin levels differentiated the PH group, which showed decreased ET-1 (SM = 22.4 +/- A 2.4 pg/ml and PH = 16.4 +/- A 1.5 pg/ml; P = 0.034) and increased ET-3 (SM = 2.1 +/- A 0.1 ng/ml and PH = 3.2 +/- A 0.1 ng/ml; P = 0.000) levels. Conclusions in patients with schistosomiasis and portal hypertension (presinusoidal type), the levels of VEGF and ET-1 differ from those reported in patients with cirrhosis.
- ItemAcesso aberto (Open Access)Endothelin-1 receptor antagonists protect the kidney against the nephrotoxicity induced by cyclosporine-A in normotensive and hypertensive rats(Assoc Bras Divulg Cientifica, 2018) Caires, A. [UNIFESP]; Fernandes, G. S. [UNIFESP]; Leme, A. M. [UNIFESP]; Castino, B.; Pessoa, E. A. [UNIFESP]; Fernandes, S. M.; Fonseca, C. D. [UNIFESP]; Vattimo, M. F.; Schor, N. [UNIFESP]; Borges, F. T. [UNIFESP]Cyclosporin-A (CsA) is an immunosuppressant associated with acute kidney injury and chronic kidney disease. Nephrotoxicity associated with CsA involves the increase in afferent and efferent arteriole resistance, decreased renal blood flow (RBF) and glomerular filtration. The aim of this study was to evaluate the effect of Endothelin-1 (ET-1) receptor blockade with bosentan (BOS) and macitentan (MAC) antagonists on altered renal function induced by CsA in normotensive and hypertensive animals. Wistar and genetically hypertensive rats (SHR) were separated into control group, CsA group that received intraperitoneal injections of CsA (40 mg/kg) for 15 days, CsA+BOS and CsA+MAC that received CsA and BOS (5 mg/kg) or MAC (25 mg/kg) by gavage for 15 days. Plasma creatinine and urea, mean arterial pressure (MAP), RBF and renal vascular resistance (RVR), and immunohistochemistry for ET-1 in the kidney cortex were measured. CsA decreased renal function, as shown by increased creatinine and urea. There was a decrease in RBF and an increase in MAP and RVR in normotensive and hypertensive animals. These effects were partially reversed by ET-1 antagonists, especially in SHR where increased ET-1 production was observed in the kidney. Most MAC effects were similar to BOS, but BOS seemed to be better at reversing cyclosporine-induced changes in renal function in hypertensive animals. The results of this work suggested the direct participation of ET-1 in renal hemodynamics changes induced by cyclosporin in normotensive and hypertensive rats. The antagonists of ET-1 MAC and BOS reversed part of these effects.
- ItemAcesso aberto (Open Access)Nitric oxide, cholesterol oxides and endothelium-dependent vasodilation in plasma of patients with essential hypertension(Associação Brasileira de Divulgação Científica, 2002-11-01) Moriel, Patricia; Sevanian, Alex; Ajzen, Sergio Aron [UNIFESP]; Zanella, Maria Teresa [UNIFESP]; Plavnik, Frida Liane [UNIFESP]; Rubbo, Homero; Abdalla, Dulcineia Saes Parra; Universidade de São Paulo (USP); University of Southern California School of Pharmacy Department of Toxicology; Universidade Federal de São Paulo (UNIFESP); Universidad de la Republica Departamento de BioquimicaThe objective of the present study was to identify disturbances of nitric oxide radical (·NO) metabolism and the formation of cholesterol oxidation products in human essential hypertension. The concentrations of·NO derivatives (nitrite, nitrate, S-nitrosothiols and nitrotyrosine), water and lipid-soluble antioxidants and cholesterol oxides were measured in plasma of 11 patients with mild essential hypertension (H: 57.8 ± 9.7 years; blood pressure, 148.3 ± 24.8/90.8 ± 10.2 mmHg) and in 11 healthy subjects (N: 48.4 ± 7.0 years; blood pressure, 119.4 ± 9.4/75.0 ± 8.0 mmHg).Nitrite, nitrate and S-nitrosothiols were measured by chemiluminescence and nitrotyrosine was determined by ELISA. Antioxidants were determined by reverse-phase HPLC and cholesterol oxides by gas chromatography. Hypertensive patients had reduced endothelium-dependent vasodilation in response to reactive hyperemia (H: 9.3 and N: 15.1% increase of diameter 90 s after hyperemia), and lower levels of ascorbate (H: 29.2 ± 26.0, N: 54.2 ± 24.9 µM), urate (H: 108.5 ± 18.9, N: 156.4 ± 26.3 µM), ß-carotene (H: 1.1 ± 0.8, N: 2.5 ± 1.2 nmol/mg cholesterol), and lycopene (H: 0.4 ± 0.2, N: 0.7 ± 0.2 nmol/mg cholesterol), in plasma, compared to normotensive subjects. The content of 7-ketocholesterol, 5alpha-cholestane-3ß,5,6ß-triol and 5,6alpha-epoxy-5alpha-cholestan-3alpha-ol in LDL, and the concentration of endothelin-1 (H: 0.9 ± 0.2, N: 0.7 ± 0.1 ng/ml) in plasma were increased in hypertensive patients. No differences were found for ·NO derivatives between groups. These data suggest that an increase in cholesterol oxidation is associated with endothelium dysfunction in essential hypertension and oxidative stress, although ·NO metabolite levels in plasma are not modified in the presence of elevated cholesterol oxides.
- ItemSomente MetadadadosRisk factors for cardiovascular disease and endothelin-1 levels in Takayasu arteritis patients(Springer, 2009-04-01) Souza, Alexandre Wagner Silva de [UNIFESP]; Mariz, Henrique Ataide [UNIFESP]; Reis Neto, Edgard Torres dos [UNIFESP]; Arraes, Anne Elizabeth Diniz [UNIFESP]; Silva, Neusa Pereira da [UNIFESP]; Sato, Emilia Inoue [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The objective of this study was to evaluate traditional risk factors for cardiovascular disease (CVD) and endothelin-1 (ET-1) levels in Takayasu arteritis (TA) patients. Twenty-two TA patients and 37 controls were evaluated. TA patients had a higher prevalence of hypertension (63.6% vs. 21.6%, p = 0.001) and higher levels of triglycerides (129.5 mg/dL +/- 70.8 vs. 88.4 mg/dL +/- 60.8, p = 0.017) than controls. Mean number of CVD risk factors was 1.64 +/- 1.22 in TA patients and 1.03 +/- 1.44 among controls, p = 0.030. More TA patients presented at least one CVD risk factor when compared to controls (77.2% vs. 51.3%, p = 0.048). ET-1 levels were higher in patients than in controls (1.49 pg/mL +/- 0.45 vs. 1.27 pg/mL +/- 0.32, p = 0.034), however no significant difference was found between patients with active and inactive disease. in this study, TA patients presented a higher prevalence of hypertension, higher levels of triglycerides, and ET-1 than controls.