Navegando por Palavras-chave "Crises Convulsivas Maternas"

Agora exibindo 1 - 1 de 1
  • Imagem de Miniatura
    Item
    Embargo
    Impacto de crises convulsivas maternas sobre o cérebro em desenvolvimento: avaliação protéica
    (Universidade Federal de São Paulo (UNIFESP), 2009-07-29) Cossa, Ana Carolina [UNIFESP]; Scerni, Debora Amado [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Purpose The aim of this work was to study the convulsive maternal seizures impact on the protein synthesis at the developing brain. Methodology Adult female Wistar rats were submitted to the induced model of epilepsy by pilocarpine and matted during the chronic phase of the model. In the first day after birth (P1), the pups of these rats (experimental group) were injected intraperitonealy with leucine L-[1-14C], as well as the pups of rats without epilepsy for control of this procedure. To verify the expression of the Bax, Bcl-2, GFAP and PARP proteins through the Western Blotting procedure, we studied the brains of experimental and control pups at the 3rd (P3), 7th (P7), 14th (P14) and 21st (P21) postnatal days. Results The experimental pups presented a reduction in the protein synthesis in distinct cerebral regions, such as, CA1, CA2, CA3, hilus, dentate gyrus, amygdala, thalamus, posterior hypothalamus, entorhinal cortex and piriform cortex. The experimental pups at P3 showed a decreased expression of Bax and Bcl-2 proteins. At P7, they showed an increased expression of PARP and GFAP proteins, as well as at P14, where the pups also showed an increase of Bax and Bcl-2 protein expression. At P21, the experimental pups presented an increase on the expression of Bax and PARP proteins and a reduction on Bcl-2 expression. Conclusion We could observe that the hypoxic-ischemic (HI) insults that the pups possibly had suffer during the intrauterine life caused by the maternal seizures, provoked a first response of decreased protein synthesis soon after birth and a late cellular death that had began at P7 and was extended until P21. The astrogliosis observed at P7 and P14 could be an reaction in response to the cellular damage. Therefore the observation of a reduced protein synthesis and the cellular death confirmed the previous results of our group that maternal epileptic seizures are able to promote HI insults at the developing brains of the pups once that, corroborating with literature, these are some of the mechanisms that occur after HI insult.