Navegando por Palavras-chave "Chondrocytes"
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- ItemAcesso aberto (Open Access)Desvendando a relação estrutura-função de condroitim sulfatos : estudo de efeitos anti-inflamatórios sobre condrócitos e macrófagos(Universidade Federal de São Paulo (UNIFESP), 2017-08-31) Cunha, Andre Luiz da [UNIFESP]; Michelacci, Yara Maria Correa da Silva [UNIFESP]; Aguiar, Jair Adriano Kopke de [UNIFESP]; http://lattes.cnpq.br/5023687526571878; http://lattes.cnpq.br/3137165519167799; http://lattes.cnpq.br/5971806359279104; Universidade Federal de São Paulo (UNIFESP)The aim of the present study was to investigate the activities of chondroitin sulfates (CS) with different structures on cultured chondrocytes and macrophages. CS were extracted and purified from bovine trachea (CSTB), porcine trachea (CSTS), chicken sternum (CSFr) and skate (CSRj) cartilage. The preparations were 90-98% pure, with ~1% proteins, nucleic acids and keratan sulfate contaminants. Structural analysis of these CS and of commercial C4S and C6S has shown that most of their disaccharides are monosulfated: C4S, CSTB, CSTS and CSFr are predominantly 4-sulfated (64-78%), while C6S is more 6-sulfated (71%) and CSRj is more heterogenous. All the CS showed minor ammounts of non-sulfated disaccharides (2-7%), and 2,6-disulfated disaccharides were detected in C6S (7%) and CSRj (14%). All CS were polydisperse, with modal molecular weights of 26-135 kDa. The different CS reduced the IL-1β-induced liberation of NO and PGE2 on both human and horse chondrocytes, and CSTB was the most effective. C4S, C6S, CSFr and CSRj reduced the LPS-induced TNF-α liberation, but not IL-6, on RAW 264.7 immortalized macrophage-like cell line. In contrast, on bone marrow derived macrophages (primary culture), C4S, C6S, CSTB and CSTS reduced the LPS-induced liberation of TNF-α, IL-6, IL-1β, and NO, indicating that the RAW response to CS was different from that of primary macrophages. All CS, except for commercial C4S, inhibited the translocation of NF-κB transcription factor to the nucleus of LPS-challenged bone marrow derived macrophages. In conclusion, our results have shown that CS with different structures had anti-inflammatory effects on macrophages, besides the already known effects on chondrocytes, suggesting that, in vivo, a synergic action of these molecules on different cell types help to achieve its desired therapeutic effects.
- ItemSomente MetadadadosEffects of phototherapy on cartilage structure and inflammatory markers in an experimental model of osteoarthritis(Spie-soc Photo-optical Instrumentation Engineers, 2013-12-01) Oliveira, Poliani de [UNIFESP]; Santos, Anderson Amaro [UNIFESP]; Rodrigues, Tamara [UNIFESP]; Tim, Carla Roberta [UNIFESP]; Pinto, Karina Zambone [UNIFESP]; Magri, Angela Maria Paiva [UNIFESP]; Fernandes, Kelly Rossetti [UNIFESP]; Mattiello, Stela Márcia [UNIFESP]; Parizotto, Nivaldo Antonio [UNIFESP]; Anibal, Fernanda Freitas [UNIFESP]; Renno, Ana Claudia Muniz [UNIFESP]; Universidade Federal de São Carlos (UFSCar); Adventist Univ Ctr São Paulo; Universidade Federal de São Paulo (UNIFESP)The aim of this study was to evaluate the effects of laser phototherapy on the degenerative modifications on the articular cartilage after the anterior cruciate ligament transection (ACLT) in the knee of rats. Eighty male rats (Wistar) were distributed into four groups: intact control group (IG), injured control group (CG), injured laser treated group at 10 J/cm(2) (L10), and injured laser treated group at 50 J/cm(2) (L50). Animals were distributed into two subgroups, sacrificed in 5 and 8 weeks postsurgery. the ACLT was used to induce knee osteoarthritis in rats. After 2 weeks postsurgery, laser phototherapy initiated and it was performed for 15 and 30 sessions. the histological findings revealed that laser irradiation, especially at 10 J/cm(2), modulated the progression of the degenerative process, showing a better cartilage structure and lower number of condrocytes compared to the other groups. Laser phototherapy was not able to decrease the degenerative process measured by Mankin score and prevent the increase of cartilage thickness related to the degenerative process. Moreover, it did not have any effect in the biomodulation of the expression of markers IL1 beta, tumor necrosis factor-alpha, and metalloprotein-13. Furthermore, laser irradiated animals, at 50 J/cm(2) showed a lower amount of collagen type 1. (C) 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
- ItemSomente MetadadadosAn evaluation of chondrocyte morphology and gene expression on superhydrophilic vertically-aligned multi-walled carbon nanotube films(Elsevier B.V., 2013-03-01) Antonioli, Eliane; Lobo, Anderson O.; Ferretti, Mario [UNIFESP]; Cohen, Moises [UNIFESP]; Marciano, Fernanda R.; Corat, Evaldo J.; Trava-Airoldi, Vladimir J.; Hosp Israelita Albert Einstein; Univ Vale Paraiba; Universidade Federal de São Paulo (UNIFESP); Inst Nacl Pesquisas EspaciaisCartilage serves as a low-friction and wear-resistant articulating surface in diarthrodial joints and is also important during early stages of bone remodeling. Recently, regenerative cartilage research has focused on combinations of cells paired with scaffolds. Superhydrophilic vertically aligned carbon nanotubes (VACNTs) are of particular interest in regenerative medicine. the aim of this study is to evaluate cell expansion of human articular chondrocytes on superhydrophilic VACNTs, as well as their morphology and gene expression. VACNT films were produced using a microwave plasma chamber on Ti substrates and submitted to an O-2 plasma treatment to make them superhydrophilic. Human chondrocytes were cultivated on superhydrophilic VACNTs up to five days. Quantitative RT-PCR was performed to measure type I and type II Collagen, Sox9, and Aggrecan mRNA expression levels. the morphology was analyzed by scanning electron microscopy (SEM) and confocal microscopy. SEM images demonstrated that superhydrophilic VACNTs permit cell growth and adhesion of human chondrocytes. the chondrocytes had an elongated morphology with some prolongations. Chondrocytes cultivated on superhydrophilic VACNTs maintain the level expression of Aggrecan, Sox9, and Collagen II determined by gPCR. This study was the first to indicate that superhydrophilic VACNTs may be used as an efficient scaffold for cartilage or bone repair. (C) 2012 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosInfluência da displasia da tróclea femoral nos resultados clínico-funcionais do implante autólogo de condrócitos em lesoes condrais da articulação femoropatelar(Universidade Federal de São Paulo (UNIFESP), 2020-07-01) Mestriner, Alexandre Barbieri [UNIFESP]; Faloppa, Flavio [UNIFESP]; Universidade Federal de São PauloBackground: Autologous chondrocyte implantation (ACI) is a viable treatment option for cartilage lesions in the patellofemoral joint (PFJ). However, the influence of trochlear dysplasia on ACI outcomes in the PFJ remains undetermined. Purpose: to evaluate the influence of trochlear dysplasia on clinical outcomes after ACI for the treatment of cartilage lesions in the PFJ with a minimum of 2 years follow-up. Methods: A retrospective review of 190 patients submitted to cartilage repair with ACI for focal cartilage defects in the PFJ by a single surgeon was performed. Patient factors, lesion morphology, and pre- and postoperative patient-reported outcomes measures (PROMs) including KOOS, Lysholm, Tegner and IKDC scores were collected. Two imaging analyzes, using knee radiography and MRI, were performed. The first analysis excluded patients with alteration in patellar height or TT-TG distance. Two independent observers determined the presence and grade of trochlear dysplasia in the second image analysis, based on Dejour and Lippacher classifications. Forty-six patients with a mean follow-up of 3.7 ± 1.9 years and mean age of 30.1 ± 8.8 were then stratified into two matched groups based on the presence or absence of trochlear dysplasia (23 vs. 23) and a comparison was established based on PROMs and failure rates. A secondary analysis excluded patients with low-grade dysplasia (Dejour type A) and established a comparison between nineteen patients with high-grade dysplasia (Dejour types B, C and D) and nineteen without trochlear dysplasia. XIX Results: Interobserver reliability (k) was 0.774 for the Dejour and 0.924 for the Lippacher classification (p < 0.001). There were no statistically significant differences in demographic characteristics between both groups (23 patients with trochlear dysplasia vs. 23 without dysplasia). PROMs at final follow-up did not differ between control and trochlear dysplasia group (p > 0.05). No difference was seen regarding failure rates between both groups (p > 0.999). Additionally, no differences in clinical outcomes were seen between patients with high-grade dysplasia (Dejour types B, C and D) and patients without dysplasia (p > 0.05). Conclusion: Trochlear dysplasia presence and severity did not influence ACI clinical outcomes in the PFJ at 3.7 years of follow-up. This study suggests that trochlear dysplasia should not be considered as a contra-indication to cartilage repair with ACI in the PFJ.