Navegando por Palavras-chave "Chemokines"
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- ItemSomente MetadadadosAcid Ceramidase Deficiency is characterized by a unique plasma cytokine and ceramide profile that is altered by therapy(Elsevier Science Bv, 2017) Dworski, Shaalee; Lu, Ping; Khan, Aneal; Maranda, Bruno; Mitchell, John J.; Parini, Rossella; Di Rocco, Maja; Hugle, Boris; Yoshimitsu, Makoto; Magnusson, Bo; Makay, Balahan; Arslan, Nur; Guelbert, Norberto; Ehlert, Karoline; Jarisch, Andrea; Gardner-Medwin, Janet; Dagher, Rawane; Terreri, Maria Teresa [UNIFESP]; Lorenco, Charles Marques; Barillas-Arias, Lilianna; Tanpaiboon, Pranoot; Solyom, Alexander; Norris, James S.; He, Xingxuan; Schuchman, Edward H.; Levade, Thierry; Medin, Jeffrey A.Acid Ceramidase Deficiency (Farber disease, FD) is an ultra-rare Lysosomal Storage Disorder that is poorly understood and often misdiagnosed as Juvenile Idiopathic Arthritis (JIA). Hallmarks of FD are accumulation of ceramides, widespread macrophage infiltration, splenomegaly, and lymphocytosis. The cytokines involved in this abnormal hematopoietic state are unknown. There are dozens of ceramide species and derivatives, but the specific ones that accumulate in FD have not been investigated. We used a multiplex assay to analyze cytokines and mass spectrometry to analyze ceramides in plasma from patients and mice with FD, controls, Farber patients treated by hematopoietic stem cell transplantation (HSCT), JIA patients, and patients with Gaucher disease. KC, MIP-1 alpha, and MCP-1 were sequentially upregulated in plasma from FD mice. MCP-1, IL-10, IL-6, IL-12, and VEGF levels were elevated in plasma from Farber patients but not in control or JIA patients. C16-Ceramide (C16-Cer) and dhC16-Cer were upregulated in plasma from FD mice. a-OH-C18-Cer, dhC12-Cer, dhC24:1-Cer, and C22:1-Cer-1P accumulated in plasma from patients with FD. Most cytokines and only a-OH-C18-Cer returned to baseline levels in HSCT-treated Farber patients. Sphingosines were not altered. Chitotriosidase activity was also relatively low. A unique cytokine and ceramide profile was seen in the plasma of Farber patients that was not observed in plasma from HSCT-treated Farber patients, JIA patients, or Gaucher patients. The cytokine profile can potentially be used to prevent misdiagnosis of Farber as JIA and to monitor the response to treatment. Further understanding of why these signaling molecules and lipids are elevated can lead to better understanding of the etiology and pathophysiology of FD and inform development of future treatments. (C) 2016 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosAre cytokines and chemokines suitable biomarkers for Takayasu arteritis?(Elsevier Science Bv, 2017) Savioli, Bruna [UNIFESP]; Abdulahad, Wayel H.; Brouwer, Elisabeth; Kallenberg, Cees G. M.; Silva de Souza, Alexandre Wagner [UNIFESP]There is a growing need for disease related biomarkers in Takayasu arteritis (TA).The assessment of pro-inflammatory cytokines and chemokines in TA may provide a better understanding of its pathophysiology, and circulating levels of these mediators may act as biomarkers of disease activity. Serum level of interleukin 6 (IL-6) is a potential biomarker for TA, which is mostly associated with TA status and disease activity. Associations between TA and serum/plasma levels of other cytokines are less clear. mRNA expression of IL-4 and tumor necrosis factor alpha (TNF alpha) are constitutively increased in peripheral blood mononuclear cells (PBMC) from TA patients and the expression of both cytokines increases even more after PBMC stimulation in vitro, while the expression of IL-10 mRNA decreases. In addition, circulating T cells from TA patients produce increased levels of both Th1- and Th17-related cytokines upon in vitro stimulation. In the aorta from TA patients, an increased expression of interferon gamma (IFN gamma), IL-6, IL-12 and IL-17 has been described. Regarding circulating chemokines in TA, serum/plasma levels of IL-8 (CXCL8), CCL2 and CCL5 were shown to be elevated in TA patients compared with healthy controls as well as in TA patients with active disease compared with those in remission. Serum IL-6 seems to be the best biomarker for disease state and disease activity in TA and increased Th1 and Th17 responses are predominant in the pathophysiology of TA (C) 2017 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosImpact of peripheral levels of chemokines, BDNF and oxidative markers on cognition in individuals with schizophrenia(Elsevier B.V., 2013-10-01) Asevedo, Elson [UNIFESP]; Gadelha, Ary [UNIFESP]; Noto, Cristiano [UNIFESP]; Mansur, Rodrigo B. [UNIFESP]; Zugman, Andre [UNIFESP]; Belangero, Sintia I. N. [UNIFESP]; Berberian, Arthur A. [UNIFESP]; Scarpato, Bruno S. [UNIFESP]; Leclerc, Emilie [UNIFESP]; Teixeira, Antonio L.; Gama, Clarissa S.; Bressan, Rodrigo A. [UNIFESP]; Brietzke, Elisa [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal de Minas Gerais (UFMG); Univ Fed Rio Grande do SulObjective: To investigate possible differences in peripheral levels of chemokines, BDNF and oxidative markers between patients with Schizophrenia (SZ) and matched healthy controls, and investigate the correlation of these biomarkers with cognitive performance.Methods: Thirty individuals with SZ and 27 healthy controls were included and the following plasmatic biomarkers' levels were determined according to manufacturers' instructions: BDNF, TBARS, protein carbonyl content (PCC) and the chemokines CXCL-10/IP-10, CXCL-8/IL-8, CCL-11, CCL-24/Eotaxin-2, CCL-2/MCP-1, CCL-3/MIP-1. Selected neuropsychological tasks were administered to assess verbal learning (Hopkins Verbal Learning Test), verbal fluency (FAS test), working memory (Visual Working Memory Task, Keep Track Task, Letter Memory Task), set shifting (Plus minus task, Number letter task), inhibition (Computerized Stroop Task, Semantic Generation Task) and complex executive function tasks (Tower of London and the shortened version of the WCST-64).Results: Compared with the healthy control group, individuals with SZ presented significantly higher levels of BDNF and the chemokine CCL-11, and lower levels of TSARS and the chemokine CXCL-10/IP-10. When we examined only the SZ group, BDNF levels were positively correlated with semantic generation tasks. Working memory ability was negatively correlated with PCC. Regarding chemokines, CCL-11 was negatively correlated to performance in working memory test, and positively correlated with cognitive flexibility task. CXCL-8/IL-8 was positively correlated with verbal fluency. CCL-24/Eotaxin-2 was positively correlated with semantic generation ability and letter memory task.Conclusions: Our results indicate that cognitive performance in SZ is associated with mediators of neuroplasticity that can be measured peripherally. (C) 2013 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosInfrared low-level diode laser on serum chemokine MCP-1 modulation in mice(Springer, 2013-02-01) Fukuda, Thiago Y. [UNIFESP]; Tanji, Maury M.; Jesus, Julio Fernandes de [UNIFESP]; Silva, Suelen Rocha da; Sato, Maria N.; Plapler, Helio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP); Univ Grande ABCThe effect of the low-level laser therapy (LLLT) in the modulation of cells related to inflammatory processes has been widely studied, with different parameters. the objective was to investigate the immediate and cumulative effect of infrared LLLT on chemokine monocyte chemotactic protein-1 (MCP-1) modulation in mice. Fifty-two isogenic mice were distributed in seven groups: control (n = 10, no surgical procedure), laser I (n = 7, surgical procedure and a single LLLT exposure 12 h after the surgery), laser II (n = 7, surgery followed by two LLLT exposures, 12 and 36 h after surgery), and laser III (n = 7, surgery followed by three LLLT exposures, 12, 36, and 60 h after surgery). for each group, a sham group (n = 21) underwent surgery without laser application. the animals in the laser groups received an infrared diode continuous laser exposure (AsGaAl, 780 nm wavelength, power of 20 mW, energy density of 10 J/cm(2), spot size of 0,04 cm(2)) on three points (20 s per point), and a final energy of 0.4 J. the animals were sacrificed 36 h (laser I and sham I groups), 60 h (laser II and sham II), and 84 h (laser III and sham III groups) after surgery. the MCP-1 concentrations were measured by cytometric bead array. There was no significant difference between the three periods in the sham group (p = 0.3). There was a lower concentration of MCP-1 in the laser III group compared to the laser I group (p = 0.05). the infrared LLLT showed a cumulative effect in the modulation of chemokine MCP-1 concentration. Three LLLT exposures were necessary to achieve the MCP-1 modulation.
- ItemAcesso aberto (Open Access)Papel da integrina LFA-1 e do receptor de quimiocina CX3CR1 na diferenciação e ativação de linfócitos T CD8+ durante a infecção experimental pelo Trypanosoma cruzi(Universidade Federal de São Paulo, 2022-05-31) Cariste, Leonardo Moro [UNIFESP]; Vasconcelos, Jose Ronnie Carvalho de [UNIFESP]; http://lattes.cnpq.br/2376141137368343; http://lattes.cnpq.br/8165078453183993; Universidade Federal de São Paulo (UNIFESP)Os linfócitos T CD8+ são cruciais no controle de infecções por patógenos intracelulares como Trypanosoma cruzi. As moléculas de quimiocinas e integrinas são essenciais para a migração dos linfócitos T. No entanto, são escassos o papel dessas moléculas durante a infecção pelo T. cruzi. Os receptores de quimiocinas CX3CR1 e CXCR3 são moléculas altamente expressas na superfície dos linfócitos T CD8+ durante a infecção por patógenos intracelulares, como T. cruzi, e são importantes na ativação e diferenciação dessas células. A integrina LFA-1 também possui papel na ativação e diferenciação dos linfócitos em células de memória, uma vez que é extremamente importante no estabelecimento do contato dos linfócitos com as células apresentadoras de antígenos. Portanto nosso objetivo foi analisar as moléculas CX3CR1, CXCR3 e LFA-1 no controle da infecção, ativação, migração e diferenciação dos linfócitos T CD8. Nossos resultados demonstram que o bloqueio do CXCR3 e/ou LFA-1 levou a um aumento na carga parasitária sanguínea e tecidual além de tornar os camundongos da linhagem C57BL/6 suscetíveis à infecção pelo T. cruzi. Além disso, o bloqueio da integrina LFA-1 diminui a função efetora, citotoxicidade e migração das células T CD8+ quando comparados ao grupo infectado. Essa diminuição da função efetora também foi observada em camundongos da linhagem OT-I quando infectados com a cepa Y-OVA e tratados com anti-LFA-1. Ainda, observamos que o bloqueio do LFA-1 altera o fenótipo das células T CD8+, uma vez que os animais infectados têm um fenótipo de células T efetoras, já os tratados com LFA-1 se assemelham ao fenótipo de células naive. Também observamos que receptor de quimiocina CX3CR1 é altamente expresso nas células T CD8, porém sua ausência não comprometeu a sobrevivência e secreção de IFN- pelas células T CD8. Em conjunto, nossos dados demonstram que o LFA-1 e CXCR3 tem um papel crítico na imunidade protetora dos animais, além disso o LFA-1 exerce um papel extremamente importante nas células T CD8 auxiliando na sua migração, diferenciação e execução da sua função efetora, permitindo assim novos estudos para vacinas direcionais, com o intuito do aumento da proteção e resposta celular.
- ItemAcesso aberto (Open Access)Peripheral chemokine levels in women with recurrent major depression with suicidal ideation(Associação Brasileira de Psiquiatria - ABP, 2012-03-01) Grassi-Oliveira, Rodrigo; Brietzke, Elisa [UNIFESP]; Teixeira, Antonio Lúcio [UNIFESP]; Pezzi, Júlio Carlos; Zanini, Márcio [UNIFESP]; Lopes, Rodrigo Pestana; Bauer, Moisés Evandro; Pontifícia Universidade Católica Post-Graduate Program in Psychology Developmental Cognitive Neuroscience Research Group; Universidade Federal de São Paulo (UNIFESP); Universidade Federal Minas Gerais School of Medicine Department of Internal Medicine; Universidade Federal de Ciências da Saúde de Porto Alegre Post-Graduate Program in Health Sciences; Pontifícia Universidade Católica Institute of Biomedical Research and Faculty of BiosciencesOBJECTIVE: To compare serum levels of MCP-1/CCL2, RANTES/CCL5, and Eotaxin/CCL11 between female patients with recurrent major depressive disorder (MDD) and healthy controls, verifying if there is a difference in the levels of these mediators between those with or without current suicidal ideation. METHODS: Thirty female outpatients with recurrent MDD were divided in two groups accordingly the presence or absence of suicidal ideation. These groups were compared with 16 healthy controls. Serum levels of MCP-1/CCL2, RANTES/CCL5, and Eotaxin/CCL11 were determined. Depression severity was evaluated by Beck Depression Inventory (BDI). Suicidal ideation was assessed by SCID-I and BDI. RESULTS: Patients with recurrent MDD and healthy controls did not differ in age, socioeconomic status, and education. All patients reported high scores of BDI (mean, SD, n; 29.75, 10.55, 28). Multivariable analysis of covariance adjusted for age and BMI showed that MDD patients with suicidal ideation presented lower levels of MCP-1/ CCL2 and RANTES/CCL5 (p < 0.001) and higher levels of Eotaxin/CCL11 (p = 0.04) compared to healthy controls. These differences remained significant after adjusting for depression severity. CONCLUSION: The findings of this study indicated that the presence of recurrent MDD with suicidal ideation is associated with differences in inflammatory chemokines when compared to those without suicidal ideation.
- ItemAcesso aberto (Open Access)The role of CD8+ T cells during allograft rejection(Associação Brasileira de Divulgação Científica, 2002-11-01) Bueno, Valquiria [UNIFESP]; Pestana, Jose Osmar Medina [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Organ transplantation can be considered as replacement therapy for patients with end-stage organ failure. The percent of one-year allograft survival has increased due, among other factors, to a better understanding of the rejection process and new immunosuppressive drugs. Immunosuppressive therapy used in transplantation prevents activation and proliferation of alloreactive T lymphocytes, although not fully preventing chronic rejection. Recognition by recipient T cells of alloantigens expressed by donor tissues initiates immune destruction of allogeneic transplants. However, there is controversy concerning the relative contribution of CD4+ and CD8+ T cells to allograft rejection. Some animal models indicate that there is an absolute requirement for CD4+ T cells in allogeneic rejection, whereas in others CD4-depleted mice reject certain types of allografts. Moreover, there is evidence that CD8+ T cells are more resistant to immunotherapy and tolerance induction protocols. An intense focal infiltration of mainly CD8+CTLA4+ T lymphocytes during kidney rejection has been described in patients. This suggests that CD8+ T cells could escape from immunosuppression and participate in the rejection process. Our group is primarily interested in the immune mechanisms involved in allograft rejection. Thus, we believe that a better understanding of the role of CD8+ T cells in allograft rejection could indicate new targets for immunotherapy in transplantation. Therefore, the objective of the present review was to focus on the role of the CD8+ T cell population in the rejection of allogeneic tissue.