Navegando por Palavras-chave "Centropomus parallelus"
Agora exibindo 1 - 6 de 6
Resultados por página
Opções de Ordenação
- ItemSomente MetadadadosCaracterização morfo-citoquímica, imunocitoquímica e ultraestrutural das células sanguíneas do robalo peva Centropomus parallelus (Poey, 1860)(Universidade Federal de São Paulo (UNIFESP), 2010-08-25) Silva, Wemeson Ferreira da [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Fifteen individuals of fat snook, Centropomus parallelus, a fish living in estuaries and lagoons in Cananéia, a coastline city in the state of São Paulo, were studied. Their morphological analysis was performed after Rosenfeld and Laür staining. The histochemical analysis included PAS, Sudan Black B, and the detection of myeloperoxidase, acidic and alkaline phosphatase, and esterase. The indirect immunohistochemical avidin-biotin-complex method was used to detect the metaloproteinases MMP2 and 9. The ultra-structural analysis was carried out on leucocytes fixed in Karnovsky fixative and processed conventionally for MET. The analysis of Centropomus parallelus blood evidenced 5 cell types: erythrocytes, thrombocytes, neutrophils, lymphocytes and monocytes. Erithrocytes were found to be either round, slightly elliptical or elliptical with their single nucleus having the same cell shape. After Laür staining, young erithrocytes (or reticulocytes), which are either round or slightly elliptical with grayish cytoplasm, showed a reticular blue staining indicative of ribonucleoprotein. Thrombocytes are round or elliptical with their nucleus having the same cell shape surrounded by a hyaline or slightly acidophilic cytoplasm, which contains glycogen and is slightly positive for esterase. Neutrophils are usually round with either a round, slanting-sided or offcentered bilobed nucleus in a clear cytoplasm frequently showing basophilic bodies. These cells were positive for glycogen, myeloperoxidase, acid phosphatase, Sudan Black B stainable cell structures, esterase and metaloproteinases 2 and 9. Limphocytes were round having a large round nucleus with slightly indented border leaving little room for basophilic cytoplasm. These cell were positive for acid phosphatase and esterase. Monocytes were round with a slightly off-centered, deeply indented slanting-sided nucleus surrounded by a basophilic cytoplasm frequently containing vacuoles. These cells were positive for acid phosphatase and esterase. Ultra-structurally, young erithrocytes showed a round nucleus whereas mature one had an elliptical nucleus, with heterochromatin predomining both surrounded by a cytoplasm of granular appearance and abundant polyribosomes – mainly in young ells – mitochondria and microtubules. Thrombocytes showed either grossly hound a slanting-sided or elongated nucleus with heterochromatin predomining, staining, nuclear pores. Their cytoplasm contained vesicles, a net of tubules (canalicular system), and occasional phagocytic debris. Neutrophils showed either a round, off centered or bilobed nucleus with core euchromatin, nuclear pores, and cytoplasm containing round granules, endocytic vesicles, rough endoplasmatic reticulum, free ribosomes and fine twisted mitochondria. Limphocytes had a round nucleus with core euchromatin leaving little room for cytoplasm containing few electron-dense round granules, few mitochondria and small electron-lucid vesicles. Monocytes showed extended cytoplasmatic projections, an euchromatic nuclear core, a thin heterochromatic layer bordering the nuclear membrane internally, an evident nucleolus, and cytoplasm containing electron-dense granules varying in size, mitochondria, vacuoles and vesicles.
- ItemSomente MetadadadosCytochemical, immunocytochemical and ultrastructural observations on leukocytes and thrombocytes of fat snook (Centropomus parallelus)(Elsevier B.V., 2011-10-01) Silva, Wemeson F. da [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Santos, Antenor A.; Antoniazzi, Marta M.; Silva, Marcelo [UNIFESP]; Gutierre, Robson C. [UNIFESP]; Paiva, Maria Jose R.; Universidade Federal de São Paulo (UNIFESP); UNASP; Butantan Inst; Fishery InstThe cytochemical, immunocytochemical and ultrastructural characteristics of leukocytes and thrombocytes in the peripheral blood of the fat snook (Centropomus paralellus) - a fish occurring in Brazil were investigated. the cytochemical methods were performed to demonstrate four enzymatic reactions o-toluidine-hydrogen peroxide, naphtol AS-MX phosphate, naphtol AS-BI phosphate and alpha-naphtil acetate to detect myeloperoxidase (MPO), alkaline phosphatase (ALP), acid phosphatase (ACP) and non-specific esterase (alpha-NAE), respectively and two non-enzymatic ones Periodic-Acid Schiff (PAS) and Sudan black B (SBB) to detect the occurrence of glycogen and phospholipids, respectively. Immunocytochemical method utilizing polyclonal rabbit antibody against mammal metalloproteinases (MMPs) 2 and 9 were done. Standard method for Electron Microscopy (EM) was applied for the ultrastructural study. the cytochemical reactions were positive in neutrophils for MPO, ACP, alpha-NAE, glycogen and phospholipids; in lymphocytes for ACP and alpha-NAE; in monocytes for ACP and alpha-NAE and in thrombocytes for ACP, alpha-NAE and glycogen. Only neutrophils were positive for MMPs 2 and 9, and none of the cells studied were positive for ALP. Ultrastructurally: 1) neutrophil showed a spherical shape with a spherical, indented or lobulated euchromatic nucleus, and cytoplasm containing granules of varied sizes and mitochondria of varied shapes and sizes. the nucleus/cytoplasm relation and the size of granules suggest neutrophil maturation in peripheral blood; 2) lymphocytes showed partially heterochromatic nucleus and minimal cytoplasm; 3) monocytes had long cytoplasmic projections, an indented nucleus, evident nucleolus and cytoplasm with granules of varied sizes and vacuoles; 4) thrombocytes were predominantly elliptical or roughly spherical in shape, had a partially heterochromatic nucleus and cytoplasm containing electron-dense granules, intricate canalicular system and vacuoles occasionally holding phagocytic material. (C) 2011 Elsevier B.V. All rights reserved.
- ItemAcesso aberto (Open Access)Estrutura do rim cefálico, hematologia e atividade fagocítica de macrófago do robalo Centropomus parallelus de cativeiro e de vida livre(Universidade Federal de São Paulo (UNIFESP), 2009-06-24) Santos, Antenor Aguiar [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The objective of this work was to determine hematological parameters and the phagocytic capacity of peritoneal macrophages of the fat snook related to sex, stage of gonadal maturation and seasonal cycle. The hematological results did not show significant differences between males and females. With respect to stage of gonadal maturation, only erythrocytes numbers (RBC), in females, was found to be significantly elevated in stage III (maturing) and decreased in V (spent). An analysis of the results of the erythrocyte and leukocyte series, thrombocytes and phagocytic activity related to sex and seasonal cycle showed statistically significant differences: a) in males, hematocrit (Ht) and hemoglobin concentration (Hb) were elevated in the spring and low in the winter; b) in both sexes, mean corpuscular volume (MCV) was high in the spring and summer and low in the fall; c) in females, thrombocytes numbers were elevated in the fall and low in the other seasons; d) in both sexes, phagocytic capacity (PC) and phagocytic index (PI) were higher in the summer and lower in the fall. The results showed that spring and summer correspond to seasons of the year for better hematological and phagocytic responses for survival of the fat snook in its natural habitat. The parameters studied could be utilized for evaluation of the health status in its own habitat or in captivity.
- ItemSomente MetadadadosHematological parameters and phagocytic activity in fat snook (Centropomus parallelus) bred in captivity(Elsevier B.V., 2012-10-01) Santos, Antenor Aguiar; Ranzani-Paiva, Maria Jose T.; Veiga, Marcelo Leite da; Faustino, Lucas; Egami, Mizue Imoto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Adventist Univ; Fishery Inst; Universidade Federal de Sergipe (UFS); Universidade de São Paulo (USP)The objective of this work was to determine the hematological parameters and the phagocytic capacity of peritoneal macrophages of fat snook related to sex, stage of gonadal maturation and seasonal cycle. Blood was collected from 135 animals (78 females and 57 males) and used for determinations of: erythrocyte number, hematocrit, hemoglobin, erythrocyte indices mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), total and differential leukocyte counts, and thrombocyte count. the phagocytic capacity and phagocytic index were determined after Saccharomyces cerevisiae inoculation in the peritoneal cavity of the animals. the hematological results according to sex showed that the erythrocyte, total leukocyte and thrombocyte counts were statistically higher in males than females, with the latter showing a higher MCV. Concerning to erythrocyte count, hematocrit and hemoglobin concentration analyzed separately by sex and stage of gonadal maturation, males were found to have significantly elevated values in the mature stage and decreased levels in the resting stage. the results of the erythrocyte and leukocyte series, thrombocytes and phagocytic activity related to seasonal cycle showed significant differences in both sexes, where hematocrit and hemoglobin concentration were lower in winter and higher in the other seasons, mean corpuscular volume was higher in the summer and lower in the winter and fall, total leukocytes and thrombocytes lower in the spring and higher in the fall, lymphocytes low in the winter and summer and high in the spring and phagocytic capacity and phagocytic index high in the summer and low in the winter and fall. the results showed that the hematological values in males are statistically higher than those in females, the erythrocyte values in males increase with the progression of gonadal maturation and that winter is the season of the year least favorable for hematological and phagocytic responses for survival of fat snook kept in captivity. the parameters studied could be utilized in the evaluation of the health status of this species in captivity. (C) 2012 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosHematological parameters and phagocytic activity in fat snook (Centropomus parallelus): Seasonal variation, sex and gonadal maturation(Elsevier B.V., 2009-11-16) Santos, Antenor Aguiar [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Ranzani-Paiva, Maria Jose T.; Juliano, Yara; Adventist Univ Ctr São Paulo UNASP; Universidade Federal de São Paulo (UNIFESP); Fishery Inst; Univ Santo AmaroThe objective of this work was to determine hematological parameters and the phagocytic capacity of peritoneal macrophages of the fat snook related to sex. stage of gonadal maturation and seasonal cycle. the hematological results did not show significant differences between males and females. With respect to stage of gonadal maturation, only erythrocytes numbers (RBC), in females, was found to be significantly elevated in stage III (maturing) and decreased in V (spent). An analysis of the results of the erythrocyte and leukocyte series, thrombocytes and phagocytic activity related to sex and seasonal cycle showed statistically significant differences: a) hematocrit (Ht) and hemoglobin concentration (Hb) wet e elevated in the spring and low in the winter in males; b) mean corpuscular volume (MCV) was high in the spring and summer and low in the fall in both sexes; c) thrombocytes numbers were elevated in the fall and low in the other seasons in females: d) phagocytic capacity (PC) and phagocytic index (PI) were higher in the summer and lower in the fill in females. the results showed that spring and summer correspond to seasons of the year for better hematological and phagocytic responses for survival of the fat snook in its natural habitat. the parameters studied could be utilized for evaluation of the health status fat snook in its own habitat or in captivity. (C) 2009 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosSpecial dyeing, histochemistry, immunohistochemistry and ultrastructure: A study of mast cells/eosinophilic granules cells (MCs/EGC) from Centropomus parallelus intestine(Academic Press Ltd- Elsevier Science Ltd, 2017) Silva, Wemeson Ferreira da [UNIFESP]; Simões, Manuel de Jesus [UNIFESP]; Gutierre, Robson Campos [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Santos, Antenor Aguiar [UNIFESP]; Antoniazzi, Marta M.; Sasso, Gisela Rodrigues da Silva [UNIFESP]; Ranzani-Paiva, Maria Jose T.Intestine mast cells/eosinophilic granule cells (MCs/EGC) of the marine species Centropomus parallelus (fat snook) were first studied using light and electron microscopy techniques. Mast cells are cells from the connective tissue found in almost all organs and tissues of vertebrates. In fish, they appear in greater numbers in parts of their bodies that are exposed to their environment, such as skin, gills and intestine. The granules in fat snook's mast cell contain a variety of substances, such as histamine, heparin, chondroitin sulfate, serotonin, proteases and cytokines. The present study of intestine MCs/EGC was carried out in 20 specimens of fat snook. Samples of tissue were fixed in Bouin solution and in buffered formalin. Ferric hematoxylin - Congo red, pH6 acridine orange, pH2.5 and pH0,5 Alcian Blue (AB), toluidine blue, PAS, AB + PAS and immunohistochemistry protocols were used. In the mucosa and submucosa layers, MCs/EGCs granules with basic contents were evidenced by Congo red staining, and with acid contents granules were identified through pH 2.5 and 0,5 AB, and acridine orange. Basic and acid contents were simultaneously evidenced using ferric hematoxylin - Congo red stain. Metachromasia was observed in both mucosal and submucosal mast cells. Neutral glycoproteins were evidenced by using PAS protocol, glycosaminoglycan through AB and both simultaneously through AB + PAS. In immunohistochemistry assays, MCs/EGC were positive for tryptase, chymase and serotonin. As in mammals, the study of samples fixed in modified Karnovsky for transmission electron microscopy evidenced that most of the MCs granules were spherical and showed varying electron density, as described in previous reports on other teleost fish species. The metachromasia observed and the identification of tryptase, chymase and serotonin suggest a great similarity between fat snook's MCs/EGC and those described in the mucosa of mammals. (C) 2016 Elsevier Ltd. All rights reserved.