Navegando por Palavras-chave "Carbapenem resistance"
Agora exibindo 1 - 6 de 6
Resultados por página
Opções de Ordenação
- ItemSomente MetadadadosCarbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil(Elsevier Brazil, 2017) Campana, Eloiza Helena [UNIFESP]; Xavier, Danilo Elias [UNIFESP]; Petrolini, Fernanda Villas-Boas [UNIFESP]; Cordeiro-Moura, Jhonatha Rodrigo [UNIFESP]; Elmor de Araujo, Maria Rita; Gales, Ana Cristina [UNIFESP]The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in Sao Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. 8-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed by PFGE. Carbapenems hydrolysis was not detected by carbapenemase assay in the carbapenem-resistant and cephalosporin-susceptible P. aueruginosa clinical isolates. OprD decreased expression was observed in all P. aeruginosa isolates by qRT-PCR. The outer membrane protein profile by SDS-PAGE suggested a change in the expression of the 46 kDa porin that could correspond to OprD porin. The isolates were clustered into 17 genotypes without predominance of a specific PFGE pattern. These results emphasize the involvement of multiple chromosomal mechanisms in carbapenem-resistance among clinical isolates of P. aeruginosa, alert for adaptation of P. aeruginosa clinical isolates under antimicrobial selective pressure and make aware of the emergence of an uncommon phenotype among P. aeruginosa clinical isolates. (C) 2016 Sociedade Brasileira de Infectologia. Published by Elsevier Editora Ltda.
- ItemSomente MetadadadosDetection of bla(VIM-7) in an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST1284 in Brazil(Elsevier Science Inc, 2017) de Paula, Suelen Balero; Cayo, Rodrigo [UNIFESP]; Streling, Ana Paula [UNIFESP]; Nodari, Carolina Silva [UNIFESP]; Matos, Adriana Pereira [UNIFESP]; Eches Perugini, Marcia Regina; Gales, Ana Cristina [UNIFESP]; Carrara-Marroni, Floristher Elaine; Yamada-Ogatta, Sueli F.We described for the first time an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST1284 carrying a plasmid-mediated bla(VIM-7) in Brazil. The bla(VIM-7) was harbored by an integron that also carried aacA4 and bla(OXA-46). Multiple virulence factors were also detected. (C) 2017 Elsevier Inc. All rights reserved.
- ItemAcesso aberto (Open Access)Emergence of Klebsiella pneumoniae-producing KPC-2 carbapenemase in Paraíba, Northeastern Brazil(Brazilian Society of Infectious Diseases, 2012-12-01) Fehlberg, Lorena Cristina Corrêa [UNIFESP]; Carvalho, Albalucia M.c.; Campana, Eloiza Helena [UNIFESP]; Gontijo-Filho, Paulo P.; Gales, Ana Cristina [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal da Paraíba Hospital Universitário Lauro Wanderley; Universidade Federal de Uberlândia Biological Sciences InstituteThe emergence of KPC-2 producing K. pneumoniae in hospitalized patients at the intensive care unit (ICU) of a teaching hospital located in the city of João Pessoa, Paraíba, Brazil, is reported. Seven carbapenem-resistant K. pneumoniae recovered from different body sites of infection were analyzed. Most isolates showed a multidrug-resistance phenotype. Genotypic analysis demonstrated the presence of two genotypes, with the predominance of genotype A, which belongs to ST 437. These isolates also carry the encoding genes of five other betalactamases.
- ItemAcesso aberto (Open Access)Metalo-beta-lactamases(Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia, 2006-04-01) Mendes, Rodrigo Elisandro [UNIFESP]; Castanheira, Mariana [UNIFESP]; Pignatari, Antonio Carlos Campos [UNIFESP]; Gales, Ana Cristina [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Laboratório ALERTA Laboratório Especial de Microbiologia ClínicaIncrease isolation of Gram-negative bacilli resistant to broad-spectrum cephalosporin has been observed during the last few years, thus determining the use of more potent beta-lactams, such as carbapenems. The use of these antimicrobial agents may lead to the emergence of carbapenem resistant Gram-negative bacilli in the nosocomial environment. Carbapenem resistance may be due to the production of Ambler class D beta-lactamase or Ambler class B beta-lactamase, also called metallo-beta-lactamase (MbetaL). Apart from the monobactam aztreonam, this class of enzyme virtually hydrolyze all the commercially available beta-lactams. Since 90s, several clinical important nosocomial microorganisms, including members of Enterobacteriaceae family, Pseudomonas spp. and Acinetobacter spp., have been found to produce MbetaLs enzymes. When these carbapenem non-susceptible strains are found, they may be submitted to phenotypic MbetaL detection test in the microbiology laboratory in order to help infection control practioners and prevent the MbetaL gene dissemination, once these genes are embedded in mobile genetic elements, which can spread rapidly to other Gram-negative species.
- ItemSomente MetadadadosMicrobiological and epidemiological characterization of imipenem-resistant Pseudomonas aeruginosa strains from a Brazilian tertiary hospital: Report from the SENTRY Antimicrobial Surveillance Program(Esift Srl, 2006-10-01) Ribeiro, Julival; Mendes, Rodrigo Elisandro [UNIFESP]; Domingos, R.; Franca, E.; Silbert, Suzane [UNIFESP]; Jones, Ronald N.; Sader, Helio Silva [UNIFESP]; Hosp Base Distrito Fed; Universidade Federal de São Paulo (UNIFESP); JMI LabsOBJECTIVES: To evaluate the antimicrobial susceptibility profile, the genetic similarity, and the mechanisms of carbapenem resistance among imipenem-resistant Pseudomonas aeruginosa isolates collected from a Brazilian tertiary teaching hospital.METHODS: Seventy-eight consecutive samples of P. aeruginosa were evaluated during 2000 and 2001. The antimicrobial susceptibility was evaluated by reference broth microdilution methods and the imipenem-resistant isolates were screened for metallo-p-lactamase (MPL) production throughout disc approximation test and MOL Etest (R) strips and isolates with positive screen test result were submitted to PCR assays using primers bla(IMP-1), bla(VIM-1), bla(VIM-2) e bla(SPM-1). The genetic similarity of MOL-producing strains was evaluated by automated ribotyping for epidemiological typing purpose.RESULTS: Resistance rates were high to the majority of antimicrobial agents tested except polymyxin B, which inhibited all samples at the Clinical and Laboratory Standards Institute breakpoint (<= 2 mu g/ml). Twenty-nine (37.2%) isolates were resistant to imipenem and these isolates showed great genomic variability. MPL production was demonstrated in two imipenem-resistant isolates, which were detected using bla(SPM-1) and bla(IMP-2)-specific primers. Sequence analysis revealed the presence of bla(SPM-1) and a novel biamp-type gene, bla(IMP)-typeCONCLUSION: The results of this study showed high resistance rates to the majority of antimicrobial agents among P. aeruginosa samples. High imipenem resistance rates were probably due to continuous selection of resistant mutants. The production of MOL did not represent a frequent mechanism of carbapenem resistance in this medical center; but a novel MPL was identified. Continued antimicrobial surveillance and infection control measures should be emphasized to minimize the emergence and dissemination of antimicrobial resistance.
- ItemSomente MetadadadosSerratia marcescens harboring SME-4 in Brazil: A silent threat(Elsevier Science Inc, 2017) Cayo, Rodrigo [UNIFESP]; Paes Leme, Rodrigo Cuiabano; Streling, Ana Paula [UNIFESP]; Matos, Adriana Pereira [UNIFESP]; Nodari, Carolina Silva [UNIFESP]; Esteves Chaves, Jessica Reis; Ferreira Brandao, Jorge Luiz; de Almeida, Maira Fernandes; Carrareto, Valerio; de Castro Pereira, Marco Aurelio; Aquino de Almeida, Jean Pierre; Ferreira, Demian Candido; Gales, Ana Cristina [UNIFESP]The intrinsic polymyxin resistance displayed by Serratia marcescens makes the acquisition of carbapenemase encoding genes a worrisome event. This study report a SME-4-producing S. marcescens isolate causing septic shock in Brazil. The insertion of novel resistance determinants and their consequent spread in our territory is noteworthy. (C) 2017 Elsevier Inc. All rights reserved.