Navegando por Palavras-chave "Cadmium"

Agora exibindo 1 - 20 de 20
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Ação Do Suco De Uva E Suco De Maçã Em Rins De Ratos Expostos Ao Cloreto De Cádmio
    (Universidade Federal de São Paulo (UNIFESP), 2018-03-12) Handan, Bianca Andrade [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Cadmium Is A Non-Essential Metal That Can Cause Several Diseases, Including Cancer. In This Study, Nutraceutical Potential Of Grape Juice (G8000") And Apple Juice Were Evaluated Against Cadmium-Induced Renal Toxicity. A Total Of 20 Male-Wistar Rats Were Distributed Into Four Groups (N=5): Control Group: Animals Received An Intraperitoneal (Ip) Injection Of 0.9% Saline Solution And After 15 Days, 1 Ml Of Water Was Administered For 15 Days, Via Gavage; Cadmium Group: Animals Received An Ip Injection Of Cadmium Chloride (1.2 Mg/Kg) And After 15 Days, 1 Ml Of Water Was Administered For 15 Days Via Gavage; Cadmium Group + Grape Juice: Animals Received An Ip Injection Of Cadmium Chloride (1.2 Mg/Kg), And After 15 Days, 0.8 Ml Of Grape Juice G8000" Was Administered For 15 Days, Via Gavage; Cadmium Group + Apple Juice: Animals Received An Ip Injection Of Cadmium Chloride (1.2 Mg/Kg) And After 15 Days, 1.0 Ml Of Apple Juice Was Administered For 15 Days, Via Gavage. A Severe Tubular Lesion With Areas Of Necrosis Was Ob
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Antimutagenic and antigenotoxic potential of grape juice concentrate in blood and liver of rats exposed to cadmium
    (Springer, 2014-11-01) Moura, Carolina Foot Gomes de [UNIFESP]; Ribeiro, Flavia Andressa Pidone [UNIFESP]; Jesus, Gustavo Protasio Pacheco de [UNIFESP]; Silva, Victor Hugo Pereira da [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Gollucke, Andrea Pittelli Boiago [UNIFESP]; Aguiar, Odair [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The aim of this study was to evaluate the antimutagenic and antigenotoxic potential of grape juice concentrate in rodent organs exposed to cadmium chloride intoxication. A total of 15 Wistar rats were distributed into three groups (n=5), as follows: control group (CTRL; nontreated group), cadmium group (Cd), and cadmium-grape juice group (Cd+GJ). Exposed animals received intraperitoneal injection of cadmium chloride (1.2 mg/kg body weight) diluted in water and, after 15 days, Cd+GJ group received grape juice concentrate for 15 days, by gavage (0.8 mL, 1.18 mg of polyphenols kg(-1) day(-1)). Grape juice concentrate was able to decrease genotoxic effects induced by cadmium in peripheral blood and liver cells as depicted by single cell gel (comet) and micronucleus assays. A decrease for anti-8-hydroxy-20-deoxyguanosine (8OHdG) expression in hepatocytes of animals exposed to cadmium and treated with grape juice concentrate was also detected. Higher CuZn-SOD activity was observed in liver cells of the Cd+GJ group. No remarkable differences were seen regarding Mn-SOD activity among groups. Taken together, our results demonstrate that grape juice concentrate was able to exert antimutagenic and antigenotoxic activities in blood and liver cells of rats exposed to cadmium.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Are food compounds able to modulate noxious activities induced by cadmium exposure?
    (Emerald Group Publishing Ltd, 2016) Moura, Carolina Foot Gomes de [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]
    Cadmium is one of the most toxic environmental and industrial pollutants and is able to induce severe injury because it is poorly excreted, accumulating in various organs. This common pollutant is responsible for serious damage in lung, brain, testis, kidney, liver, circulatory system, and bone. Food compounds, such as flavonoids, represent the most abundant polyphenols in human diet and comprise thousands of substances, which are freely available as high-dose dietary supplements. The mechanism of action of these ones consists in free radical scavenging and metal sequestration. The interaction of metal ions with flavonoids leads to chelation and the use of these natural compounds is better than the synthetic ones due to their lower toxic effects. The aim of this review is to describe the role of some food compounds, focusing on flavonoids for modulating noxious biological activities induced by cadmium exposure.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Association between heavy metal exposure and poor working memory and possible mediation effect of antioxidant defenses during aging
    (Elsevier Science Bv, 2017) Souza-Talarico, Juliana Nery; Marcourakis, Tania; Barbosa, Fernando, Jr.; Moraes Barros, Silvia Berlanga; Rivelli, Diogo Pineda; Pompeia, Sabine [UNIFESP]; Caramelli, Paulo; Plusquellec, Pierrich; Lupien, Sonia J.; Catucci, Raphael Fernandes; Alves, Andrea Regiani; Suchecki, Deborah [UNIFESP]
    Background: Inverse associations have been observed between memory performance and blood concentrations of cadmium (Cd) and lead (Pb). Low antioxidant cell activity has also been linked to decline in memory due to aging. However, it has not yet been established whether the heavy metal-memory relationship is mediated by differences in antioxidant activity. Methods: We examined Cd and Pb levels, as well as oxidative stress parameters, in blood samples from 125 older adults (age range 50-82 years). The Counting Span Test (CST) was used to evaluate working memory capacity (WMC). The Monte Carlo Method for Assessing Mediation (MCMAM) was used to analyze the mediation role of antioxidant activity in the heavy metals-memory association. Results: High blood Cd (BCd) concentration alone, and in combination with elevated blood Pb (BPb) concentration, was associated with poor WMC (p = 0.001) and low enzymatic antioxidant defenses (p = 0.006). The variance in WMC accounted for by BCd or by BCd combine with BPb was 20.6% and 18.6%, respectively. The MCMAM revealed that the influence of BCd and BPb concentrations on WMC was mediated by low antioxidant capacity (confidence interval -CI: 0.072 to -0.064 for BCd
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Avaliação do potencial nutracêutico de extrato de cenoura roxa (daucus carota lSspSativus varAtrorubens alef) em múltiplos tecidos (sangue periférico, medula óssea e fígado) de ratos expostos ao cloreto de cádmio
    (Universidade Federal de São Paulo (UNIFESP), 2016-03-31) Claudio, Saumel Rangel [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The aim of this study was to investigate if purple carrot extract is able to protect against the noxious activities induced by cadmium exposure in multiple organs of rats. For this purpose, histopathological analysis, genotoxicity and oxidative status were investigated in this setting. A total of 20 Wistar rats weighing 250g on the average, and 8 weeks age were distributed into four groups (n=5), as follows: Control group (non-treated group, CTRL); Cadmium group (Cd) and Purple carrot extract groups at 400 mg/L or 800 mg/L. Histopathological analysis revealed that liver from animals treated with purple carrot extract improved tissue degeneration induced by cadmium intoxication. Genetic damage was reduced in blood and hepatocytes as depicted by comet and micronucleus assays in animals treated with purple carrot extract. SOD-CuZn and cytocrome C gene expression increased in groups treated with purple carrot extract. Purple carrot extract also reduced the 8OHdG levels and catalase expression in liver cells when compared to cadmium group. Taken together, our results demonstrate that purple carrot extract is able to protect against cadmium intoxication by means of reducing tissue regeneration, genotoxicity and oxidative stress in multiple organs of Wistar rats.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Cadmium-induced testicular injury
    (Elsevier B.V., 2009-08-01) Siu, Erica Rosanna; Mruk, Dolores D.; Porto, Catarina Segreti [UNIFESP]; Cheng, C. Yan; Populat Council; Universidade Federal de São Paulo (UNIFESP)
    Cadmium (Cd) is an environmental toxicant and an endocrine disrupter in humans and rodents. Several organs (e.g., kidney, liver) are affected by Cd and recent studies have illustrated that the testis is exceedingly sensitive to Cd toxicity. More important, Cd and other toxicants, such as heavy metals (e.g., lead, mercury) and estrogenic-based compounds (e.g., bisphenols) may account for the recent declining fertility in men among developed countries by reducing sperm count and testis function. in this review, we critically discuss recent data in the field that have demonstrated the Cd-induced toxicity to the testis is probably the result of interactions of a complex network of causes. This is likely to involve the disruption of the blood-testis barrier (BTB) via specific signal transduction pathways and signaling molecules, such as p38 mitogen-activated protein kinase (MAPK). We also summarize current studies on factors that confer and/or regulate the testis sensitivity to Cd, such as Cd transporters and metallothioneins, the impact of Cd on the testis as an endocrine disruptor and oxidative stress inducer, and how it may disrupt the Zn(2+) and/or Ca(2+) mediated cellular events. While much work is needed before a unified mechanistic pathway of Cd-induced testicular toxicity emerges, recent studies have helped to identify some of the likely mechanisms and/or events that take place during Cd-induced testis injury. Furthermore, some of the recent studies have shed lights on potential therapeutic or preventive approaches that can be developed in future studies by blocking or minimizing the destructive effects of Cd to testicular function in men. (C) 2009 Elsevier Inc. All rights reserved
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Determinação de cádmio, crômio e mercúrio por espectrometria de fluorescência de raios X em cooperativas de reciclagem da Região Metropolitana de São Paulo
    (Universidade Federal de São Paulo, 2015-05-15) Vieira, Willian de Oliveira Novais [UNIFESP]; Assunção, Nilson Antonio de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Throughout history humans have been exposed to various elements present in nature and substances derived from their activities, with the technological advances and the possibility of electronic purchase this exhibition became pronounced, especially in recycling cooperatives where the amount of such equipment It is high. For lack of information handling them inappropriately collectors materials and also do not use the proper protective equipment which enhances exposure to toxic waste. The amount, the route and the exposure period are decisive factors in determining the risk that each element is to the health of workers and can cause acute or chronic poisoning. We evaluated the concentration of metals: cadmium, chromium and mercury in 18 recycling cooperatives in the metropolitan region of São Paulo. These elements were chosen because of their impact on the environment; particularly industrialized regions like the city of Diadema and present a major risk to human health. Analyses were carried out in partnership with the GEA Institute - Ethics and Environment, and the elements were determined by fluorescence spectrometry X-rays, using portable instrument. The metals were determined in soils, workspaces and public contact sites in cooperatives, and their concentrations ranged from 7 ± 1-5706 ± 811 (ppm). In some analyzes it was observed standard deviations above average, suggesting occasional contamination. In general, the concentrations of metals in the samples are above the levels permitted or recommended by national and international standards, and in some cases, there are differences between the regulatory agencies as the maximum tolerable limits, but there is agreement on the potential risk exposure the metals poses to human health and the environment.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Determination of Cd, Cr and Pb in phosphate fertilizers by laser-induced breakdown spectroscopy
    (Elsevier B.V., 2014-07-01) Nunes, Lidiane Cristina; Arantes de Carvalho, Gabriel Gustinelli; Santos Junior, Dario [UNIFESP]; Krug, Francisco Jose; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    A validated method for quantitative determination of Cd, Cr, and Pb in phosphate fertilizers by laser-induced breakdown spectroscopy (LIBS) is presented. Laboratory samples were comminuted and homogenized by cryogenic or planetary ball milling, pressed into pellets and analyzed by LIBS. the experimental setup was designed by using a Q-switched Nd:YAG at 1064 nm with 10 Hz repetition rate, and the intensity signals from Cd II 214.441 nm, Cr II 267.716 nm and Pb II 220.353 nm emission lines were measured by using a spectrometer furnished with an intensified charge-coupled device. LIBS parameters (laser fluence, lens-to-sample distance, delay time, integration time gate, number of sites and number of laser pulses per site) were chosen after univariate experiments with a pellet of NIST SRM 695 (Trace Elements in Multi-Nutrient Fertilizer). Calibration and validation were carried out with 30 fertilizer samples from single superphosphate, triple superphosphate, monoammonium phosphate, and NPK mixtures. Good results were obtained by using 30 pulses of 50 J cm(-2) (750 mu m spot size), 2.0 mu s delay time and 5.0 mu s integration time gate. No significant differences between Cd, Cr, and Pb mass fractions determined by the proposed LIBS method and by ICP OES after microwave-assisted acid digestion (AOAC 2006.03 Official Method) were found at 95% confidence level. the limits of detection of 1 mg kg(-1) Cd, 2 mg kg(-1) Cr and 15 mg kg(-1) Pb and the precision (coefficients of variation of results ranging from 2% to 15%) indicate that the proposed LIES method can be recommended for the determination of these analytes in phosphate fertilizers. (C) 2014 Elsevier B.V. All rights reserved.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Efeitos de cádmio na barreira hematotesticualr e do estrogeno em espermatozóide de rato.
    (Universidade Federal de São Paulo (UNIFESP), 2009) Siu, Erica Rosanna [UNIFESP]; Porto, Catarina Segreti [UNIFESP]
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Efeitos do consumo da polpa de juçara (euterpe edulis mart) liofilizada sobre os danos induzidos pelo cádmio em parâmetros reprodutivos de ratos wistar
    (Universidade Federal de São Paulo (UNIFESP), 2017-09-15) Araujo, Bruna Medeiros de [UNIFESP]; Aguiar, Odair [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Aims : Analyze the effects of consumption of juçara pulp on reproductive parameters of rats exposed to single intraperitoneal injection of cadmium chloride by. Methods: Treatment (56 days) using 32 adult Wistar rats distributed in: group CTRL (saline 0.9% i.p., control diet); Cd (1.2 mg / kg CdCl2 i.p., Control diet); CdJU (1.2 mg / kg CdCl2 i.p., Diet supplemented with 0.5% lyophilized juçara pulp) and JU (0.9% i.p. saline, Diet supplemented with 0.5% lyophilized juçara pulp). The animals were submitted to a single intraperitoneal injection on the 1st day of treatment (90 days of age), and euthanized on the 56th day of treatment (146 days of age). The parameters analyzed were : body mass gain, mass of reproductive organs and accessory glands, daily sperm production, sperm morphology, histopathological and testicular histomorphometric characteristics, liver histopathology and testicular activities of antioxidant enzymes (CAT and SOD). Results: There were no significant diferences related to gain of body mass in either group. The groups Cd and CdJU presented a reduction of the relative mass of the epididymis (p = 0.001), testes (p = 0.001),ventral prostate (p = 0.001) and seminal vesicle (p = 0.003), daily sperm production (p = 0.001), percentage of normal spermatozoa (p = 0.001), reduction in tubular diameter and area (p = 0.001), and a decrease in plasma testosterone levels (p = 0.004). The testes also presented several signs of tissue degeneration (p <0.05). In accordance with the activity of the total and mitochondrial SOD enzyme, there was no significant significant difference between the groups, however, an increase of catalase activity (p = 0.0192) was observed in the animals exposed to cadmium. In all analyzes, the JU group presented no significant difference when compared to the control group. Conclusion: Cadmium was efficient in altering most of the analyzed parameters. The lyophilized juçara pulp, howeever was not able to reverse or attenuate the altered parameters, because its antioxidant content may not have been enough to combat the damage caused by the metal.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Os efeitos do extrato de castanha do Brasil (bertholletia excelsa) sobre os danos induzidos pelo cádmio nos parâmetros reprodutivos de ratos machos wistar
    (Universidade Federal de São Paulo (UNIFESP), 2015-12-21) Juca, Andrea Marcelino de Oliveira [UNIFESP]; Aguiar, Odair [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Cadmium is a heavy metal which is a major environmental toxic agent found in batteries, coating against corrosion and paint pigments. Many studies have shown that testis are sensitive to cadmium toxicity, leading to a decrease of male fertility. Researchers have observed an improved quality of semen with the use of dietary substances with antioxidant activity. Among them, the selenium possesses antioxidant activity and it is present in Brazil nuts. Aim: To analyze the effects of the extract from Brazil nuts on male rats reproductive parameters intoxicated by cadmium. Methods: We used 40 male Wistar rats, distributed into four experimental groups: CRTL (intraperitoneal injection of saline solution 0.9% and daily gavage with water 1.5mL), CAD (intraperitoneal injection of 1.2mg/kg of weight CdCl2 and daily gavage with water, 1.5mL), CAD+ECB (intraperitoneal injection of 1.2mg/kg of weight CdCl2 and daily gavage with Brazil nuts, 1.5mL) and ECB (intraperitoneal injection of saline solution 0.9% and daily gavage with Brazil nuts, 1.5mL). The treatment was made for 56 days. The following parameters were assessed: body weight gain, reproductive organs relative weights and glandules accessories, morphology and daily sperm production, histopathology for testis, morphometric analysis (tubular diameter and tubular area percentage), serum levels of testosterone and enzymatic activity of glutathione, catalase and superoxido dismutase. Results: After 56 days we observed increase in body weight for the groups which received Brazil nuts extract (p=0,001). The groups exposed to cadmium and treated with cadmium and Brazil nuts showed a decrease in gonadosomatic axis (p=0,001), relative weight dorsolateral prostate (p=0,001), relative weight of epididymis (p=0,001), daily sperm production (p=0,001), numbers of spermatozoa from epididymis tail and head/body segment (p=0,001) and sperm transit time through the epididymis (p=0,004) when compared to control group. It was observed an increase in catalase activity (p= 0,183), percentage of abnormal sperm in groups intoxicated with or treated with Brazil nut extract (p=0,001). It was noticed tissue degeneration when histopathological and morphometric analyses were performed for all groups exposed to cadmium. Brazil nut treatment was not able to reverse the morphologic alterations induced by cadmium. No significant statistically differences were detected to glutathione peroxidase activity (p=0,897) among groups. However, superoxide dismutase showed a decrease activity for all groups treated with Brazil nut (p=0,019). The level of testosterone serum increased in groups treated with Brazil nuts extract compared to the non-treated groups (p=0,009). Conclusion: The extract Brazil nuts were not able to attenuate or reverse cadmium injury in male rats reproductive parameters in a medium-term essay.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Evaluation of laser induced breakdown spectroscopy for cadmium determination in soils
    (Elsevier B.V., 2009-10-01) Santos, Dario [UNIFESP]; Nunes, Lidiane C.; Trevizan, Lilian C.; Godoi, Quienly; Leme, Flavio O.; Braga, Jez Willian B.; Krug, Francisco Jose; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP); Universidade Federal de São Carlos (UFSCar); Universidade de Brasília (UnB)
    Cadmium is known to be a toxic agent that accumulates in the living organisms and present high toxicity potential over lifetime. Efforts towards the development of methods for microanalysis of environmental samples, including the determination of this element by graphite furnace atomic absorption spectrometry (GFAAS). inductively coupled plasma optical emission spectrometry (ICP OES), and inductively coupled plasma-mass spectrometry (ICP-MS) techniques, have been increasing. Laser induced breakdown spectroscopy (UBS) is an emerging technique dedicated to microanalysis and there is a lack of information dealing with the determination of cadmium. the aim of this work is to demonstrate the feasibility of LIBS for cadmium detection in soils. the experimental setup was designed using a laser Q-switched (Nd:YAG, 10 Hz, lambda = 1064 nm) and the emission signals were collimated by lenses into an optical fiber Coupled to a high-resolution intensified charge-coupled device (ICCD)-echelle spectrometer. Samples were cryogenically ground and thereafter pelletized before LIBS analysis. Best results were achieved by exploring a test portion (i.e. sampling spots) with larger surface area, which contributes to diminish the uncertainty due to element specific microheterogeneity. Calibration curves for cadmium determination were achieved using certified reference materials. the metrological figures of merit indicate that LIBS can be recommended for screening of cadmium contamination in soils. (C) 2009 Elsevier B.V. All rights reserved.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Grape Juice Concentrate Protects Rat Liver Against Cadmium Intoxication: Histopathology, Cytochrome C and Metalloproteinases Expression
    (Georg Thieme Verlag Kg, 2016) Moura, Carolina Foot Gomes de [UNIFESP]; Ribeiro, Flavia Andressa Pidone [UNIFESP]; Handan, Bianca Andrade [UNIFESP]; Aguiar, Odair [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]
    The aim of this study was to investigate if grape juice concentrate is able to protect rat liver against cadmium toxicity. For this purpose, histopathological analysis, cytochrome C expression and immunoexpresssion of metalloproteinases (MMP) 2 and 9 were investigated. A total of 15 Wistar rats weighing 250 g on the average, and 8 weeks age were distributed into 3 groups (n = 5), as follows: Control group (non-treated group, CTRL)
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Grape juice concentrate protects reproductive parameters of male rats against cadmium-induced damage: a chronic assay
    (Cambridge Univ Press, 2013-12-14) Pires, Vanessa Cardoso [UNIFESP]; Boiago Golluecke, Andrea Pittelli; Ribeiro, Daniel Araki [UNIFESP]; Lungato, Lisandro [UNIFESP]; D'Almeida, Vania [UNIFESP]; Aguiar, Odair [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ Catolica Santos
    The aim of the present study was to investigate the effects of long-term grape juice concentrate (GJC) consumption, in two dosages, on the reproductive parameters of cadmium-exposed male rats. the effects of the concentrate on body mass gain, plasma testosterone levels, reproductive organ weights, daily sperm production, sperm morphology, testis histopathological and histomorphometrical parameters, and testicular antioxidant markers were investigated. Wistar rats (n 54) were distributed into six groups: CdCl2; cadmium and grape juice I (1.18 g/kg per d); cadmium and grape juice II (2.36 g/kg per d); grape juice I (1.18 g/kg per d); grape juice II (2.36 g/kg per d); control. A single dose of CdCl2 (1.2 mg/kg body weight (BW)) was injected intraperitoneally and the grape juice was administered orally for 56 d. the results indicated that cadmium changed all reproductive and antioxidant parameters. At dosage I (1.18 g/kg BW), GJC consumption did not show the effects against cadmium-induced damages. in contrast, at dosage II (2.36 g/kg BW), the GJC improved the gonadosomatic index (P=0.003), serum testosterone levels (P=0.001), the relative weight of epididymis (P=0.013) and ventral prostate (P=0.052), the percentage of normal sperm (P=0.001), and histopathological and histomorphometrical parameters. in addition, at this dosage, normalisation of the enzymatic activity of superoxide dismutase (P=0.001) and of testicular levels of glutathione (P=0.03) were observed. the parameters of the non-exposed rats did not depict significant alterations. in conclusion, the product was able to act as a protector of reproductive function against cadmium-induced damage. Such a property was expressed in a dose-dependent manner as the more effective dose was dosage II. the GJC acted possibly by antioxidant mechanisms.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Grape skin extract mitigates tissue degeneration, genotoxicity, and oxidative status in multiple organs of rats exposed to cadmium
    (Lippincott Williams & Wilkins, 2018) Gollucke, Andrea Pittelli Boiago [UNIFESP]; Claudio, Samuel Rangel [UNIFESP]; Yamamura, Hirochi [UNIFESP]; Morais, Damila Rodrigues; Bataglion, Giovana Anceski; Eberlin, Marcos Nogueira; Aguiar, Odair [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]
    The aim of this study was to investigate whether grape skin extract can mitigate the noxious activities induced by cadmium exposure in multiple organs of rats. For this purpose, histopathological analysis for the liver, genotoxicity, and oxidative status in the blood and liver were investigated in this setting. A total of 20 Wistar rats weighing 250 g, on average, and 8 weeks of age were distributed into four groups (n=5) as follows: control group (nontreated group); cadmium group (Cd); and grape skin extract groups (Cd+GS) at 175 or 350 mg/l. Histopathological analysis in liver showed that animals treated with grape skin extract showed improved tissue degeneration induced by cadmium intoxication. Genetic damage was reduced in blood and hepatocytes as indicated by comet and micronucleus assays in animals treated with grape skin extract. Copper–zinc superoxide dismutase and cytochrome c gene expression increased in groups treated with grape skin extract in liver cells. Grape skin extract also reduced the 8-hydroxy-2′-deoxyguanosine levels in liver cells compared with the cadmium group. Taken together, our results indicate that grape skin extract can mitigate tissue degeneration, genotoxicity, and oxidative stress induced by cadmium exposure in multiple organs of Wistar rats
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Investigação do potencial preventivo do extrato de maçã contra os danos induzidos pelo cádmio em parâmetros reprodutivos de ratos wistar
    (Universidade Federal de São Paulo (UNIFESP), 2016-11-28) Schonhaus, Juliana Parusia [UNIFESP]; Aguiar, Odair [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Aims: Check the effects of previous consumption of apple extract on reproductive parameters of cadmium-exposed male rats. Methods: A total of 40 male adult Wistar rats (90 days) were distributed in the following groups: Control (CTRL) (without treatment); Cadmium (CD); Apple extract (M) (1ml/day); Cadmium and apple extract (CDM) (1ml/day). The administration of cadmium chloride (1,2 mg/kg) occurred only once, via intraperitoneal injection on the 21st day of design, and the apple extract daily by gavage for 28 days (21 days before and 7 after intoxication). The parameters analyzed were: body mass gain, weight of reproductive organs and accessory glands, daily sperm production, sperm motility, testicular histopathological and histomorphometric characteristics, testicular antioxidant markers (CAT and SOD) and formation of 8-hydroxy-2'deoxyguanosine (8-OHdG). Results: None of the groups presented differences in relation to weight gain. The groups intoxicated with cadmium, independent of the previous administration of apple extract, presented reduction in the relative mass of the epididymis (p=0,001), ventral prostate (p=0,041), dorso-lateral prostate (p=0,001), seminal vesicle (p=0,002) and testis (p=0,001), daily sperm production (p=0,001), serum testosterone levels (p=0,001), diameter and tubular área (p=0,001). Sperm motility was characterized by a significant reduction in spermatozoa classified as Type A (mobile, with progressive trajectory) in groups also poisoned by metal (p=0,006) and consequent increase in spermatozoa classified as Type C (immobile) (p=0,003). Histopathological changes were observed in all groups exposed to the metal, also showing no influence of the apple extract in these parameters (p=0.001 for CD and p=0.005 for CDM). There was no significant difference in any group regarding the enzymatic activity of CAT and total SOD. However, it was evidenced a reduction in the enzymatic activity of the mitochondrial fraction of SOD in the CD group (p=0.001) and a larger reduction in the CDM group in relation to the CD group (p=0.001). The 8-OHdG immunoexpression was increased in the Cd intoxicated groups (p=0.001 for CD and p=0.007 for CDM). Conclusions: The previously offered dose of apple extract (1/mL/day) was ineffective in protecting the male reproductive system against damage induced by single dose intraperitoneal cadmium chloride (1,2 mg/kg).
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Investigação do potencial terapêutico da vitamina E contra os danos causados pelo cádmio em parâmetros reprodutivos masculinos em ratos Wistar
    (Universidade Federal de São Paulo, 2022-01-28) Campelo, Lilian Piccolo [UNIFESP]; Aguiar, Odair [UNIFESP]; Nascimento, Leonardo Parreira Silva [UNIFESP]; http://lattes.cnpq.br/7726936511719417; http://lattes.cnpq.br/0398348332863521; http://lattes.cnpq.br/0825512823720535; Universidade Federal de São Paulo (UNIFESP)
    A infertilidade masculina tem se mostrado cada vez mais frequente em casais inférteis, sendo as causas tanto endógenas quanto exógenas. Muitos agentes tóxicos são capazes de atacar o sistema reprodutor masculino, dentre essas substâncias destaca- se o cádmio. Este metal está presente em concentrações importantes nas culturas agrícolas, no cigarro, em solos, na água e em resíduos industriais. O cádmio atua nos testículos causando ação devastadora, mesmo que em pequenas quantidades, sendo capaz de alterar a espermatogênese, e outros parâmetros reprodutivos. Este metal incita alterações importantes no epitélio seminífero e no compartimento intersticial, que levam a desordem na espermatogênese e na produção hormonal, levando à infertilidade. Há pesquisas que comprovam os malefícios gerados pela intoxicação do cádmio que diretamente desencadeia processos oxidativos, levando muitas vezes a morte celular. Inúmeros trabalhos na literatura mostram uma busca incessante por substâncias antioxidantes que possam combater ou proteger contra esses efeitos destrutivos do cádmio. Os danos oxidativos podem ser tratados com agentes antioxidantes, como os ácidos graxos poli-insaturados e as vitaminas. A vitamina E, já é conhecida por ser um poderoso antioxidante, sendo assim um forte protetor celular. Nossos objetivos implicaram em caracterizar o tratamento com a vitamina E, aferindo parâmetros reprodutivos e o status de marcadores antioxidantes testiculares, em animais expostos ao cloreto de cádmio. No presente trabalho, a vitamina E (tocoferol) foi testada em 20 ratos Wistar machos (10 ratos no grupo controle, e em 10 animais no grupo onde administramos aos 90 dias de idade uma única injeção intraperitoneal de cloreto de cádmio). Após 7 dias, foram tratados com vitamina E diluída em óleo de milho, através de gavagem oral diária com dosagem de 100 mg/kg. Após 28 dias, os animais foram eutanasiados. O objetivo deste trabalho foi caracterizar o tratamento com vitamina E, com avaliação dos parâmetros reprodutivos e marcadores da condição testicular, em animais previamente expostos ao cádmio. O cádmio promoveu danos testiculares e no sistema reprodutivo como um todo, e não houve efeito positivo do tratamento de vitamina E na concentração e na forma utilizada neste estudo.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Mimosa (Mimosa caesalpiniifolia) prevents oxidative DNA damage induced by cadmium exposure in Wistar rats
    (Informa Healthcare, 2014-12-01) Silva, Marcelo Jose Dias; Vilegas, Wagner; Silva, Marcelo Aparecido da; Moura, Carolina Foot Gomes de [UNIFESP]; Ribeiro, Flavia Andressa Pidone [UNIFESP]; Silva, Victor Hugo Pereira da [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; São Paulo State Univ; Univ Fed Alfenas; Universidade Federal de São Paulo (UNIFESP)
    The Mimosa (Mimosa caesalpiniifolia) is a plant native from South America; it is used in the traditional medicine systems for treating bacterial, fungal, parasitic and inflammatory conditions. the aim of this study was to evaluate the antigenotoxic and antioxidant activities induced by mimosa (M. caesalpiniifolia) in multiple rodent organs subjected to intoxication with cadmium chloride. A total of 40 Wistar rats (8 weeks old, 250 g) were distributed into eight groups (n = 5), as follows: Control group (non-treated group, CTRL); Cadmium exposed group (Cd); cadmium exposure and treated with extract at 62.5 mg/kg/day; cadmium exposure and treated with extract at 125 mg/kg/day; cadmium exposure and treated with extract at 250 mg/kg/day; cadmium exposure and treated with ethyl acetate fraction at 62.5 mg/kg/day. for evaluating the toxicogenetic potential of mimosa, two groups were included in the study being treated with extract at 250 mg/kg/day and acetate fraction of mimosa at 62 mg/kg/day, only. Extract of mimosa at concentrations of 62.5 and 125mg decreased DNA damage in animals intoxicated with cadmium when compared to cadmium group. in a similar manner, treatment with ethyl acetate fraction of mimosa at 62.5mg concentration in animals previously exposed to cadmium reduced genetic damage in peripheral blood cells. in a similar manner, the treatment with ethyl acetate fraction reduced DNA damage in liver cells. Oxidative DNA damage was reduced to animals exposed to cadmium and treated with 125mg of extract as well as those intoxicated to cadmium and treated with 62.5 of acetate fraction of mimosa. Taken together, our results indicate that mimosa prevents genotoxicity induced by cadmium exposure in liver and peripheral blood cells of rats as a result of antioxidant activity.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Potencial terapêutico da Mimosa caesalpiniifolia bentham em fígado de ratos expostos ao cloreto de cádmio
    (Universidade Federal de São Paulo (UNIFESP), 2019-03-18) Lima, Eliene Cezario De [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Cadmium is an environmental pollutant of great relevance to public health since it is a non-essential metal toxic to the human body. Exposure to cadmium occurs primarily through smoking and intake of contaminated food. Mimosa caesalpiniifolia Bentham (Mimosa) is a plant rich in polyphenols with antioxidant properties. This study evaluated the action of Mimosa on the liver of rats exposed to cadmium chloride, under morphological, oxidative and mutagenic parameters. A total of 40 Wistar male rats (90 days, ~ 250 g) were distributed into eight groups (n = 5), as follows: i) control: submitted to 0.9% saline injection (0.5 mL) and treated with tap water ; (ii) cadmium: cadmium chloride injection at 1.2 mg/kg and tap water; iii) Mimosa extract: submitted to 0.9% saline solution (0.5 mL) injection and treatment with Mimosa extract at 250 mg/kg; iv) Mimosa fraction: submitted to 0.9% saline injection and treatment with Mimosa acetate fraction at 62.5 mg/kg; v) cadmium and Mimosa extract 62.5: submitted to cadmium chloride at 1.2 mg/kg injection and treatment with Mimosa extract at 62.5 mg/kg; vi) cadmium and Mimosa extract 125: subjected to cadmium chloride at 1.2 mg/kg injection and treatment with Mimosa extract at 125 mg/kg; (vii) cadmium and Mimosa 250 extract: submitted to cadmium chloride 1.2 mg/kg injection and treatment with Mimosa extract at 250 mg/kg; (viii) cadmium treated with fraction of Mimosa acetate: submitted to cadmium chloride 1.2 mg/kg injection and treatment with acetate fraction of Mimosa extract at 62.5 mg /kg. Saline solution and cadmium were given as a single intraperitoneal dose on day zero and, after 15 days, the rats received the treatments described above every day via gavage for 15 days. The animals intoxicated with cadmium and treated with the fraction [62,5] had increased expression of SOD-Mn, the frequency of binucleated hepatocytes, caryolysis and karyorrhexis. The extract [62,5] was cytoprotective, antimutagenic and reduced karyolysis. The extract [125] was cytoprotective, antioxidant, antifibrotic and decrease the frequency of binucleated hepatocytes, and the extract [250] was cytotoxic and mutagenic. Our results confirmed the cytoprotective, antimutagenic, antioxidant and antifibrotic potential of Mimosa in liver of rats exposed to cadmium chloride.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Purple carrot extract protects against cadmium intoxication in multiple organs of rats: Genotoxicity, oxidative stress and tissue morphology analyses
    (Elsevier Gmbh, Urban & Fischer Verlag, 2016) Claudio, Samuel Range| [UNIFESP]; Yamamura, Hirochi [UNIFESP]; Morais, Damila Rodrigues; Bataglion, Giovana Anceski; Eberlin, Marcos Nogueira; Peres, Rogerio Correa [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Gollucke, Andrea Pittelli Boiago [UNIFESP]
    The aim of this study was to investigate if purple carrot extract is able to protect against the noxious activities induced by cadmium exposure in multiple organs of rats. For this purpose, histopathological analysis, genotoxicity and oxidative status were investigated in this setting. A total of twenty Wistar rats weighing 250 g on the average, and 8 weeks age were distributed into four groups (n=5), as follows: Control group (non-treated group, CTRL) Cadmium group (Cd) and Purple carrot extract groups at 400 mg/L or 800 mg/L. Histopathological analysis revealed that liver from animals treated with purple carrot extract improved tissue degeneration induced by cadmium intoxication. Genetic damage was reduced in blood and hepatocytes as depicted by comet and micronucleus assays in animals treated with purple carrot extract. SOD-CuZn and cytocrome C gene expression increased in groups treated with purple carrot extract. Purple carrot extract also reduced the 80HdG levels in liver cells when compared to cadmium group. Taken together, our results demonstrate that purple carrot extract is able to protect against cadmium intoxication by means of reducing tissue regeneration, genotoxicity and oxidative stress in multiple organs of Wistar rats.