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- ItemAcesso aberto (Open Access)Biomonitoramento citogenético em indivíduos expostos a derivados do petróleo: aplicação do teste do micronúcleo em células da mucosa bucal(Universidade Federal de São Paulo (UNIFESP), 2009-12-15) Martins, Renato de Almeida [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; http://lattes.cnpq.br/9969803499258672; http://lattes.cnpq.br/8345973190145546; Universidade Federal de São Paulo (UNIFESP)Devido à influência da geno-e citotoxicidade sobre a carcinogênese química, estudos têm demonstrado que derivados de petróleo são capazes de induzir danos genéticos e morte celular com resultados conflitantes até agora. O objetivo do presente estudo foi avaliar comparativamente danos ao DNA (micronúcleo) e morte celular (picnose , cariorréxis e cariólise) em células esfoliadas da mucosa oral de atendentes de postos de combustível usando dois diferentes sítios anatômicos da mucosa bucal: mucosa jugal e borda lateral da língua. Um total de 23 voluntários atendentes de postos de combustível e 23 voluntários saudáveis como controles (indivíduos não expostos) foram incluídos nesta definição. Os indivíduos tinham células do epiteliais da mucosa jugal e borda lateral da língua esfoliadas mecanicamente, colocadas em solução fixadora e, posteriormente, em lâminas limpas para a verificação ao microscópio dos fenótipos nucleares acima. Os resultados apontaram diferenças estatisticamente significantes (p <0,05) de células micronucleadas da mucosa oral de atendentes de postos de combustível para ambos os sítios avaliados. Da mesma forma, a exposição aos derivados de petróleo foi capaz de aumentar as outras alterações nucleares estreitamente relacionadas, tais como a citotoxicidade picnose, cariorréxis e cariólise, sendo o mais pronunciado efeitos aqueles encontrados para borda lateral da língua. Não houve interação entre tabagismo e exposição ao derivado de petróleo. Em resumo, estes dados indicam que os atendentes de postos de combustível compreendem um grupo de alto risco para danos ao DNA e morte celular. Parece que a borda lateral da língua é um sítio mais sensível ao geno-e citotóxica induzida por derivados petróleo.
- ItemSomente MetadadadosBiomonitoring of oral epithelial cells in petrol station attendants: Comparison between buccal mucosa and lateral border of the tongue(Elsevier B.V., 2009-10-01) Martins, Renato de Almeida [UNIFESP]; Gomes, Guilherme Augusto da Silva [UNIFESP]; Aguiar, Odair [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Owing to the influence of geno- and cytotoxicity on chemical carcinogenesis, studies have demonstrated that petroleum derivatives are able to induce genetic damage and cellular death with conflicting results so far. the aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from gas petrol attendants using two different anatomic buccal sites: cheek mucosa and lateral border of the tongue. A total of 23 gas petrol attendants and 23 health controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek and lateral border of the tongue mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the above nuclear phenotypes. the results pointed out significant statistical differences (p<0.05) of micronucleated oral mucosa cells from gas petrol attendants for both oral sites evaluated. in the same way, petroleum derivate exposure was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis, being the most pronunciated effects as those found in the lateral border of the tongue. No interaction was observed between smoking and petroleum exposure. in summary, these data indicate that gas petrol attendants comprise a high risk group for DNA damage and cellular death. It seems that the lateral border of the tongue is a more sensitive site to geno- and cytotoxic insult induced by petroleum derivates. (C) 2009 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosBiomonitoring of oral epithelial cells in smokers and non-smokers submitted to panoramic X-ray: comparison between buccal mucosa and lateral border of the tongue(Springer, 2010-12-01) Angelieri, Fernanda; Goncalves Moleirinho, Tatiana de Cassia; Carlin, Viviane [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); São Paulo Metodista UnivThe aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated oral mucosa cells from smokers and non-smokers submitted to dental X-ray using two anatomic sites: buccal mucosa and lateral border of the tongue. A total of 15 heavy smokers and 17 non-smokers were submitted to panoramic dental radiography for orthodontic reasons. Individuals had epithelial cells from cheek and lateral border of the tongue mechanically exfoliated, placed in fixative, and dropped in clean slides which were checked for the above nuclear phenotypes. the results pointed out no significant statistically differences (p > 0.05) of micronucleated oral mucosa cells before versus after X-ray exposure for both oral sites evaluated either to smokers or to non-smokers. X-ray exposure was able to increase other nuclear alterations closely related to cytotoxicity such as karrhyorexis, pyknosis, and karyolysis for two groups evaluated. Nevertheless, the most pronunciated effects were found to lateral border of the tongue of smokers. in summary, these data indicate that panoramic X-ray is able to induce cellular death in oral mucosa cells. It seems that lateral border of the tongue is more sensitive site to cytotoxic insult induced by ionizing radiation combined with continuous cigarette smoke exposure.
- ItemSomente MetadadadosCellular death but not genetic damage in oral mucosa cells after exposure to digital lateral radiography(Springer, 2011-06-01) Ribeiro, Daniel Araki [UNIFESP]; Sannomiya, Eduardo Kazuo; Pozzi, Renan [UNIFESP]; Miranda, Sandra Regina [UNIFESP]; Angelieri, Fernanda; Universidade Federal de São Paulo (UNIFESP); São Paulo Methodist UnivThe aim of the present study was to evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated buccal mucosa cells from individuals following digital lateral radiography. A total of 30 healthy patients (15 men and 15 women) indicated to the orthodontic therapy were submitted to digital lateral X-ray. Exfoliated oral mucosa cells were collected immediately before the X-ray exposure and after 10 days. the results pointed out no significant statistically differences (p > 0.05) of micronucleated oral mucosa cells. On the other hand, X-ray was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis, and karyolysis. in summary, these data indicate that exposure to digital lateral radiography may not be a factor that induced chromosomal damage, but it is able to promote cytotoxicity.
- ItemSomente MetadadadosChromosome breakage and cellular death are induced in oral epithelial cells of hairdressers: a preliminary study(Informa Healthcare, 2013-02-01) Carlin, Viviane [UNIFESP]; Fracalossi, Ana Carolina Cuzzuol [UNIFESP]; Miranda, Sandra Regina [UNIFESP]; Noguti, Juliana [UNIFESP]; Silva, Victor Hugo Pereira da [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The aim of the present study was to comparatively evaluate genomic damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from hairdressers using two different anatomic buccal sites: cheek mucosa and lateral border of the tongue. A total of 28 hairdressers and 30 health controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from the cheek and lateral border of the tongue mechanically exfoliated, placed in fixative and dropped in clean slides that were checked for the previously mentioned nuclear phenotypes. the results pointed out statistically significant differences ( p < 0.05) of micronucleated oral mucosa cells from hairdressers in the lateral border of the tongue. Exposure to hair dyes caused an increase of other nuclear alterations closely related to cytotoxicity, such as karrhyorexis, pyknosis and karyolysis in both the oral sites evaluated. in summary, these data indicate that hairdressers are occupationally exposed to agents that are genotoxic and cytotoxic. It seems that the lateral border of the tongue is a more sensitive site to the genotoxic and cytotoxic effects of hair dyes.
- ItemSomente MetadadadosChromosome damage and cytotoxicity in oral mucosa cells after 2 months of exposure to anabolic steroids (decadurabolin and winstrol) in weight lifting(Elsevier B.V., 2010-12-01) Martins, Renato de Almeida [UNIFESP]; Gomes, Guilherme Augusto da Silva [UNIFESP]; Aguiar, Odair [UNIFESP]; Medalha, Carla Christina [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The aim of the present study was to evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated buccal mucosa cells from anabolic steroid users after 2 months of exposure. Two experimental groups consisting of 15 adult males who practise weight lifting and are anabolic steroid users or 15 adult males who practise weight lifting, but are non-anabolic steroid users, were recruited. in addition, 20 sedentary males, who do not practise any physical activity regularly, were matched by age with experimental groups. No significant statistical differences (p > 0.05) were noticed in individuals who practise physical activity only. On the other hand, an increase of micronucleated cells (MNCs) in anabolic steroid (decadurabulin and Winstrol) users was observed. Regarding cytotoxic parameters, the same observation has occurred, that is, significant statistical differences (p < 0.05) were noticed in the group exposed to anabolic steroids when compared with other controls, as depicted by high frequencies of pyknosis, karyolysis and karyorrhexis. Taken together, our results suggest that genomic instability and cytotoxicity are induced by anabolic steroid administration in oral mucosa cells as assessed by the micronucleus test. (C) 2010 Elsevier Inc. All rights reserved.
- ItemAcesso aberto (Open Access)Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning(E H Angle Education Research Foundation, Inc, 2012-07-01) Lorenzoni, Diego Coelho; Fracalossi, Ana Carolina Cuzzuol [UNIFESP]; Carlin, Viviane [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Sant' Anna, Eduardo Franzotti; Universidade Federal do Rio de Janeiro (UFRJ); Universidade Federal de São Paulo (UNIFESP)Objectives: To evaluate the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated buccal mucosa cells from children undergoing orthodontic radiographs.Materials and Methods: A total of 25 healthy children undergoing orthodontic therapy partook in a complete set of orthodontic radiographs (lateral cephalographic, posteroanterior cephalographic, panoramic, full periapical exam, and bitewing). the micronucleus test in the buccal exfoliated cells was applied. the paired-samples t-test and the Wilcoxon test were used to compare the frequencies of alterations before and after X-ray exposure.Results: We found no statistically significant differences (P > .05) between micronucleated buccal mucosa cells before and after exposure to radiation. However, radiation did cause other nuclear alterations closely related to cytotoxicity (P = .007).Conclusion: According to the micronucleus test, the complete set of radiographs requested in the orthodontic planning may not be a factor that induces chromosomal damage, but it is able to promote cytotoxicity. (Angle Orthod. 2012;82:585-590.)
- ItemSomente MetadadadosCytogenetic biomonitoring in patients exposed to dental X-rays: comparison between adults and children(British Inst Radiology, 2008-10-01) Ribeiro, Daniel Araki [UNIFESP]; Oliveira, Gabriela Rodrigues de; Castro, Glaucia Monteiro de [UNIFESP]; Angelieri, Fernanda; Universidade Federal de São Paulo (UNIFESP); Univ Metodista São PauloObjectives: the aim of the present study was to comparatively evaluate the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) of exfoliated buccal mucosa cells from children and adults following dental X-ray exposure.Methods: A total of 17 adults and 17 children submitted to panoramic dental radiography were included in this study.Results: the results indicated no statistically significant differences (P > 0.05) in children's micronucleated oral mucosa cells before and after dental X-ray exposure. in the same way, no mutagenic effects were observed in adults following X-ray exposure. On the other hand, X-rays increased other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis in both groups. the comparative analysis between children and adults demonstrated no statistically significant differences in micronucleus frequency or (P > 0.05).Conclusions: in summary, these data indicate that dental paroramic radiography may not be a factor that includes chromosomal damage, but it is able to promote cytotoxicity. It seems that children are not more susceptible to the noxious activities induced by X-rays when compared with adults.
- ItemSomente MetadadadosCytogenetic biomonitoring of oral mucosa cells from adults exposed to dental X-rays(Springer, 2008-07-01) Ribeiro, Daniel Araki [UNIFESP]; Angelieri, Fernanda; Universidade Federal de São Paulo (UNIFESP); Univ Metodista São PauloPurpose. Although it has been clearly demonstrated that X-rays play a key role in diagnosing medical and dental problems, this type of ionizing radiation is also able to induce noxious activities, such as genetic damage. the aim of the present study was to evaluate DNA damage (micronucleus) and cellular death in exfoliated buccal mucosa cells from healthy individuals (smokers and nonsmokers) following dental X-ray exposure.Material and methods. A total of 39 healthy people who had submitted to panoramic dental radiography were included in the study: 9 smokers and 30 nonsmokers.Results. the results indicated no significant statistically differences (P > 0.05) in micronucleated oral mucosa cells before and after dental X-ray exposure. On the other hand, X-ray exposure did increase other nuclear alterations closely related to cytotoxicity, such as karyorrhexis, pyknosis, and karyolysis. It seems that cigarette smoke did not affect X-ray outcomes induced in buccal cells.Conclusion. These data indicate that dental panoramic radiography may not induce chromosomal damage, but it is able to promote cytotoxicity. Because cellular death is considered a prime mechanism in nongenotoxic mechanisms of carcinogenesis, dental X-ray should be used only when necessary.
- ItemSomente MetadadadosCytogenetic biomonitoring of peripheral blood and oral mucosa cells from car painters(Informa Healthcare, 2012-09-01) Silva, Victor Hugo Pereira da [UNIFESP]; Moura, Carolina Foot Gomes de [UNIFESP]; Spadari-Bratfisch, Regina Celia [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The aim of the present study was to comparatively evaluate genomic damage and cellular death in exfoliated oral mucosa cells and peripheral blood from car painters. A total of 24 car painters and 19 healthy controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek mucosa (left and right side) mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the specific nuclear phenotypes. A total of 5 mu L from peripheral blood was collected for the single cell gel (comet) assay. the results pointed out statistically significant differences (p < 0.05) of micronucleated oral mucosa cells from car painters. in addition, DNA damage was detected in peripheral blood cells by single cell gel (comet) assay. Nevertheless, exposure to car paints did not cause increases other nuclear alterations closely related to cytotoxicity such as karrhyorexis, pyknosis and karyolysis in buccal mucosa cells. in summary, the results of the present study suggest that car painters comprise a high risk group since paints can induce genotoxic and mutagenic effects in peripheral blood and oral mucosa cells, respectively.
- ItemSomente MetadadadosCytogenetic damage induced by mouthrinses formulations in vivo and in vitro(Springer, 2012-06-01) Carlin, Viviane [UNIFESP]; Matsumoto, Mariza A.; Saraiva, Patricia P.; Artioli, Andre; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); USCThe aim of the present study was to comparatively evaluate DNA damage and cellular death in cells exposed to various commercially available mouthrinses: ListerineA (R) CepacolA (R), Plax alcohol freeA (R), PeriogardA (R), and Plax WhiteningA (R). A total of 75 volunteers were included in the search distributed into five groups containing 15 people each for in vivo study. Exfoliated buccal mucosa cells were collected immediately before mouthrinse exposure and after 2 weeks. Furthermore, blood samples were obtained from three healthy donors for in vitro study. the micronucleus test was used to evaluate mutagenicity and cytotoxicity in vivo. the single-cell gel (comet) assay was used to determine DNA damage in vitro. After 2 weeks exposure, PeriogardA (R) showed 1.8% of micronucleated cells with significant statistical differences ( < 0.05) compared to before exposure (0.27%). Plax WhiteningA (R) presented high tail moment value (4.5) when compared to negative control (0.6). the addition of all mouthrinses to cells incubated with methyl methanesulfonate did not alter the number of strand breaks in the genetic material. ListerineA (R) was able to reduce genetic damage induced by hydrogen peroxide because a decrease of tail moment was noticed. the results of the present study suggest that PeriogardA (R) and Plax WhiteningA (R) can induce genetic damage, whereas ListerineA (R) is an antioxidant agent. Since DNA damage is considered to be prime mechanism during chemical carcinogenesis, these data may be relevant in risk assessment for protecting human health and preventing carcinogenesis.
- ItemSomente MetadadadosCytogenetical damage in exfoliated oral mucosa cells in elderly people suffering denture stomatitis(Wiley-Blackwell, 2010-09-01) Matsumoto, Mariza Akemi; Castanho, Juliane; Kawakami, Roberto Yoshio; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)Objectives:The aim of this study was to evaluate comparatively the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from chronic denture stomatitis patients and healthy controls.Background:Over the course of ageing, individuals may develop many diseases such as denture stomatitis.Material and methods:A total of 23 chronic denture stomatitis patients and 23 controls presenting good oral conditions were included in this study. Individuals had epithelial cells mechanically exfoliated, placed in fixative and placed on clean slides, which were checked for nuclear phenotypes.Results:The results indicated no statistically significant differences (p > 0.05) of micronucleated oral mucosa cells from chronic denture stomatitis patients when compared to healthy controls. Nevertheless, chronic denture stomatitis was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis as depicted by significant differences (p < 0.05) between groups. No interaction was observed between smoking and chronic denture stomatitis.Conclusion:In summary, these data indicated that chronic denture stomatitis was able to induce cytotoxic effects as assessed by a micronucleus test.
- ItemSomente MetadadadosDNA damage and cellular death in oral mucosa cells of children who have undergone panoramic dental radiography(Springer, 2007-06-01) Angelieri, Fernanda; Oliveira, Gabriela Rodrigues de; Sannomiya, Eduardo Kazuo; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)Background Despite wide use as a diagnostic tool in medical and dental practice, radiography can induce cytotoxic effects and genetic damage.Objective To evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated buccal mucosa cells taken from healthy children following exposure to radiation during dental radiography.Materials and methods A total of 17 children who had undergone panoramic dental radiography were included.Results We found no statistically significant differences (P > 0.05) between micronucleated oral mucosa cells in children before and after exposure to radiation. On the other hand, radiation did cause other nuclear alterations closely related to cytotoxicity including karyorrhexis, pyknosis and karyolysis.Conclusion Taken together, these results indicate that panoramic dental radiography might not induce chromosomal damage, but may be cytotoxic. Overall, the results reinforce the importance of evaluating the health side effects of radiography and contribute to the micronucleus database, which will improve our understanding and practice of this methodology in children.
- ItemSomente MetadadadosDNA damage in lymphocytes and buccal mucosa cells of children with malignant tumours undergoing chemotherapy(Springer, 2008-06-01) Minicucci, Eliana Maria; Ribeiro, Daniel Araki [UNIFESP]; Camargo, Beatriz de; Costa, Maria C.; Ribeiro, Lucia Regina; Salvadori, Daisy Maria Favero; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); AC Camargo HospThe aim of the present study was to evaluate DNA damage (micronucleus) in cytokinesis-blocked lymphocytes and exfoliated buccal mucosa cells from children with malignant tumours and under chemotherapy. Micronucleated cells (MNCs) were assessed from children before and during chemotherapy. A total of 21 healthy children (controls), matched for gender and age, were used as control. the results pointed out higher frequencies of micronucleated lymphocytes in children with malignant tumour before any therapy when compared to healthy probands. Furthermore an increase of micronucleated lymphocytes during chemotherapy was detected when compared to the data obtained before chemotherapy. No statistically significant increases of MNCs were noticed in buccal mucosa cells at any of the timepoints evaluated. Taken together, these data indicate that the presence of malignant tumours may increase the frequency of DNA damage in circulating lymphocytes, these cells being more sensitive for detecting chromosome aberrations caused by anti-cancer drugs.
- ItemAcesso aberto (Open Access)Mutagenicity and cytotoxicity in patients submitted to ionizing radiation A comparison between cone beam computed tomography and radiographs for orthodontic treatment(E H Angle Education Research Foundation, Inc, 2013-01-01) Lorenzoni, Diego Coelho; Fracalossi, Ana Carolina Cuzzuol [UNIFESP]; Carlin, Viviane [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Sant'Anna, Eduardo Franzotti; Universidade Federal do Rio de Janeiro (UFRJ); Universidade Federal de São Paulo (UNIFESP)Objectives: To evaluate and compare mutagenicity (micronucleus) and cytotoxicity (karyorrhexis, pyknosis, and karyolysis) in exfoliated buccal mucosa cells of children following cone beam computed tomography (CBCT) or conventional radiograph exposure necessary for orthodontic planning.Materials and Methods: A total of 49 healthy children were submitted to CBCT or a conventional orthodontic radiographic protocol; they were divided into two groups based on exam: CBCT (n = 24) and Radiographic Set (n = 25) groups. the micronucleus test in the exfoliated buccal mucosa cells was applied.Results: There was not a statistically significant difference (P > .05) found between the number of micronucleated buccal mucosa cells (MNC) before and after exposure to radiation in either group, showing that neither group experienced a mutagenic effect. However, radiation did cause other nuclear alterations closely related to cytotoxicity, including karyorrhexis, pyknosis, and karyolysis, in both groups (P < .05). the CBCT group presented a greater increase in cell death than was noted in the Radiographic Set group (P < .044).Conclusion: According to the micronucleus test, mutagenicity was not induced by the CBCT or the conventional radiographs, but cytotoxicity was verified after these exams, especially after CBCT. That might have happened once the CBCT group received a greater radiation dose than the Radiographic Set group as a result of the protocols used in orthodontic planning for this study. (Angle Orthod. 2013;83:104-109.)