Navegando por Palavras-chave "BrdU"

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    Influence of chronic cocaine treatment and sleep deprivation on sexual behavior and neurogenesis of the male rat
    (Elsevier B.V., 2007-08-15) Andersen, Monica Levy [UNIFESP]; Perry, Juliana Cini [UNIFESP]; Bignotto, Magda [UNIFESP]; Perez-Mendes, Patricia [UNIFESP]; Cinini, Simone Maria [UNIFESP]; Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]; Tufik, Sergio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The present study investigated the influence of chronic cocaine treatment on genital reflexes associated with paradoxical sleep deprivation (PSD), and possible alterations in hippocampus neurogenesis of the male rat. At 21 days of age, the rats were distributed into two groups and injected with saline or cocaine (7 mg/kg, three times a week for 12 weeks). At age 90 days, they were submitted to a four-day period of PSD (PSD groups) or maintained in home-cages (control groups), challenged with saline or cocaine administration, and placed in observation cages to assess genital reflexes. Two additional groups were used to quantify neurogenesis. PSD rats treated chronically with cocaine and challenged with saline did not differ from their respective control groups. the association of PSD with cocaine potentiated penile erection (PE) when compared to PSD-saline (saline challenged) rats, and these effects were similar to those observed in long-term cocaine treated rats. the bromodeoxyuridine (BrdU) assay indicated a reduction in BrdU-positive cells in the adult hippocampus after chronic cocaine treatment. These findings show that long-term cocaine treatment from brain development through adulthood had a marked effect on sexual responses and neuronal proliferation. (C) 2007 Elsevier Inc. All rights reserved.
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    Neonatal inflammatory pain increases hippocampal neurogenesis in rat pups
    (Elsevier B.V., 2011-08-26) Leslie, Ana Teresa Figueiredo Stochero [UNIFESP]; Akers, Katherine G.; Martinez-Canabal, Alonso; Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]; Covolan, Luciene [UNIFESP]; Guinsburg, Ruth [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Hosp Sick Children; Univ Toronto
    Preterm infants undergo several painful procedures during their stay in neonatal intensive care units. Previous studies suggest that early painful experiences may have an impact on brain development. Here, we used an animal model to investigate the effect of neonatal pain on the generation of new neurons in the dentate gyrus region of the hippocampus. Rat pups received intraplantar injections of complete Freund's adjuvant (CFA), a painful inflammatory agent, on either PI or P8 and were sacrificed on P22. We found that rat pups injected with CFA on P8 had more BrdU-labeled cells and a higher density of cells expressing doublecortin (DCX) in the subgranular zone of the dentate gyrus. No change in BrdU-labeling or DCX expression was observed in pups injected with CFA on P1. These findings indicate that neonatal pain can increase hippocampal neurogenesis, suggesting that early painful experiences may shape brain development and thereby influence behavioral outcome. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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    Neurogenesis induced by seizures in the dentate gyrus is not related to mossy fiber sprouting but is age dependent in developing rats
    (Academia Brasileira de Neurologia - ABNEURO, 2008-12-01) Sanabria, Yaima Del Carmen Garrido [UNIFESP]; Argañaraz, Gustavo Adolfo [UNIFESP]; Lima, Eliângela de [UNIFESP]; Priel, Margareth Rose [UNIFESP]; Trindade, Edvaldo da Silva [UNIFESP]; Loeb, Luana Mazzacoratti [UNIFESP]; Scorza, Fulvio Alexandre [UNIFESP]; Cavalheiro, Esper Abrão [UNIFESP]; Amado, Débora [UNIFESP]; Naffah-Mazzacoratti, Maria da Graca [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Neurogenesis in the dentate gyrus (DG) has attracted attention since abnormal supragranular mossy fiber sprouting occurs in the same region, in temporal lobe epilepsy. Thus, we submitted developing rats to pilocarpine-induced status epilepticus (SE) to study the relationship between neurogenesis and mossy fiber sprouting. Groups were submitted to SE at: I-P9, II-P7, P8 and P9, III-P17 e IV-P21. Neurogenesis was quantified using BrdU protocol and confirmed through double staining, using neuronal pentraxin. Other animals were monitored by video system until P120 and their brain was studied (Timm and Nissl staining). The neurogenesis at P17 (p=0.007) and P21 (p=0.006) were increased. However, only P21 group showed recurrent seizures and the mossy fiber sprouting in the same region, during adult life, while P17 did not. Thus, our results suggest that neurogenesis is not related to mossy fiber sprouting neither to recurrent spontaneous seizures in pilocarpine model.