Navegando por Palavras-chave "Bone Marrow"
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- ItemRestritoAvaliação da Expressão da Vimentina em Leucemia Linfocítica Crônica e sua importância como fator de prognóstico(Universidade Federal de São Paulo (UNIFESP), 2010-02-24) Siufi, Grazziella Curado [UNIFESP]; Yamamoto, Mihoko [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Purpose: In this study our purpose was to evaluate the prognostic significance of the vimentin expression on CLL cells, from peripheral blood (PB) and bone marrow (BM) biopsy samples, correlating with already well known prognostic markers in CLL such as mutational status of IgVH genes, expression of CD38, Zap-70 protein, and VEGFR, molecular cytogenetics (FISH) results and with Binet’s clinical stage and progression free survival (PFS) Methods: Sixty six patients with CLL from Hospital São Paulo-UNIFESP were studied. The CLL diagnosis was established by leukemic cells immunophenotyping using score system (Moreau et al). PB cells were collected at admission and evaluated by flow cytometry for CD38 (through positive cells percentage and mean fluorescence intensity –MFI- methods) and Zap-70, VEGFR and Vimentin expressions. For the last ones (Vimentin and VEGFR) mononuclear cells separated by Ficoll Hypaque gradient were used. The Vimentin expression was evaluated in fresh and/or cryopreserved blood samples and also in BM biopsy specimens obtained at the time of diagnosis, by immunohistochemistry.The BM infiltration pattern was also evaluated. The IgVH genes mutational status was evaluated by PCR techniques followed by sequencing. Results: Vimentin expression in both circulating and marrow cells was associated with VEGFR positive expression, unmutated IgVH genes, diffuse bone marrow infiltration and unfavourable cytogenetics abnormalities. Association of CD38 expression was observed only in BM. Advanced clinical stage, age>68 years, positive expression for CD38 and VEGFR, unmutated IgVH genes, unfavourable cytogenetics abnormalities, vimentin expression in PB and BM cells and diffuse marrow infiltration pattern were associated with short PFS. The impact of these prognostic markers was also observed in Binet A patients. In multivariate analysis, the Binet’s stages B+C (p=0,008), strong unimodal and bimodal IMF pattern of CD38 (p=0,03), diffuse marrow infiltration (p=0,02) and vimentin expression on BM cells by immunohistochemistry (p=0,002) were identified as independent risk factor for reduced PFS. Comparison of Vimentin expression in PB cells by flow cytometry and in BM cells by immunohistochemistry from the same patients, showed concordant results. When vimentin expression in fresh and thawed samples from the same patients was compared, concordant results were also observed. Conclusions: Vimentin expression in CLL cells from both PB and BM showed high impact as prognostic marker, predicting poor PFS, even in Binet A patients. Both flow cytometry and immunohistochemistry are relatively easy techniques and feasible in most flow cytometric or pathological laboratories, requiring the usual routine equipments. Our results were comparable to IgVH mutational status suggesting the usefulness of Vimentin as prognostic marker in CLL patients. In addition its evaluation might be useful for the better understanding of pathofisiology of CLL and also, for the therapeutic interventions, such as the use of EGCG (epigallocatechin galate – green tea).
- ItemSomente MetadadadosChallenges facing the use of endothelial progenitor cells in stem cell therapies(Assoc Arquivos Neuro- Psiquiatria, 2016) Franca, Carolina N. [UNIFESP]; Amaral, Jonatas B. [UNIFESP]; Tuleta, Izabela D.; Siviero, Fabio; Ferreira, Carlos E. S. [UNIFESP]; Izar, Maria C. O. [UNIFESP]; Fonseca, Francisco A. H. [UNIFESP]The paper summarizes the difficulties to study the rare population of endothelial progenitor cells in clinical trials, based on the experience of our group in many publications in this area.
- ItemAcesso aberto (Open Access)Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2009-10-01) Eça, Lilian Piñero Marcolin; Ramalho, Renata Belmonte; Oliveira, Isis Sousa [UNIFESP]; Gomes, Paulo Oliveira [UNIFESP]; Pontes, Paulo [UNIFESP]; Ferreira, Alice Teixeira [UNIFESP]; Mazzetti, Marcelo Paulo Vaccari [UNIFESP]; Stem Cell Research Institute; Sacred Heart University; Universidade Federal de São Paulo (UNIFESP); Lusiada University of Santos Department Plastic SurgeryPURPOSE: To assess the technique for the collection of rabbit bone marrow stem cells from different regions to be used as an experimental model in regenerative medicine. METHODS: Thirty rabbits were allocated into 2 groups: GROUP A, n=8, animals that underwent bone marrow blood (BMB) harvesting from the iliac crest; and GROUP B: including 22 rabbits that underwent BMB harvesting from the femur epiphysis. After harvesting, mononuclear cells were isolated by density gradient centrifugation (Ficoll - Histopaque). The number of mononuclear cells per ml was counted in a Neubauer chamber and cell viability was checked through Tripan Blue method. RESULTS: Harvesting from the iliac crest yielded an average of 1 ml of BMB and 3,6.10(6) cells/ml over 1 hour of surgery, whereas an average of 3ml of BMB and 11,79.10(6) cells./ml were obtained in 30 min from the femur epiphysis with a reduced animal death rate. CONCLUSION: The analysis for the obtention of a larger number of mononuclear cells/ml from rabbit bone marrow blood was more satisfactory in the femur epiphysis than in the iliac crest.
- ItemSomente MetadadadosTransplante Autólogo De Células Tronco Hematopoiéticas Periféricas Com A Presença De Neuroblastos Citodiferenciados Na Medula Óssea, Ao Final Da Terapia De Indução, Em Crianças Com Neuroblastoma Estádio 4(Universidade Federal de São Paulo (UNIFESP), 2018-04-26) Jacob, Marcella Baggiani Antonelli [UNIFESP]; Caran, Eliana Maria Monteiro [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Background: The Major Metastatic Site Of Neuroblastoma Is The Bone Marrow, In These Cases De Long-Term Survival Is Less Than 50%. Therapeutic Consolidation Is Performed With Myeloablative Therapy Followed By Autologous Transplantation And Improves The Outcome. Classically, The Opportunity To Perform The Consolidation Treatment Is Restricted To Cases In Which The Bone Marrow Does Not Show Metastatic Cells, Including Differentiating Neuroblast, At The End Of The Induction. However, The Presence Of Differentiated Neuroblasts In The Bone Marrow Would Be An Impediment To The Collection Of Peripheral Stem Cells In Patients With Neuroblastoma? Purpose: Characterize The Epidemiological Profile And Survival Of Patients With High-Risk Neuroblastoma Who Had Bone Marrow Involvement At Diagnostic, With Emphasis On Patients With Differentiating Neuroblast In The Bone Marrow That Precedes Transplantation. Methods: Retrospective Analysis Of Patients With High-Risk Neuroblastoma Who Had Bone Marrow Involvement At Diagnostic A