Navegando por Palavras-chave "Biosurfactant"
Agora exibindo 1 - 8 de 8
Resultados por página
Opções de Ordenação
- ItemSomente MetadadadosAvaliação do potencial biossurfactante de isolados do gênero Bacillus, obtidos de processos de compostagem(Universidade Federal de São Paulo (UNIFESP), 2020-07-07) Barros, Caroline De Almeida [UNIFESP]; Vasconcellos, Suzan Pantaroto de [UNIFESP]; Universidade Federal de São PauloBiosurfactants are produced by microorganisms and due to their physicalchemical properties, such as the ability to emulsify and stabilize non-miscible fluids, they have several applications in the industry, including the food, pharmaceutical, cosmetic, and environmental contexts, such as in bioremediation from hydrocarbon contaminated sites. In this sense, the search for new species of microorganisms that produce hydrocarbon-degrading biosurfactants is growing, covering the most diverse areas and specialties, making it a promising alternative, since such biocomposites have low production costs and are toxic to the environment. For this purpose, in the present study, the emulsification potential and surface activity of 24 bacterial isolates obtained from the composting process, adopted at the Fundação Parque Zoológico de São Paulo (FPZSP), were evaluated. It was possible to select 4 (four) isolates (84, 1609, 1539 and 1594), regarding the emulsifying potential, based on calculations of the emulsification index (E24) on diesel oil. The same isolates also stood out in terms of the surface activity assessed by tensiometric measurements, via the drop collapse method and Ring Method. Values close to 31 mN / m were observed for such bacterial isolates, proving their ability to break the tension between fluid interfaces. In terms of reaction yield, the same microorganisms showed an average production of 5 g of tensoactive compounds with biosurfactant activity, for each 1 g of cultured cells. Characterization analyzes of the isolates, based on DNA extraction, amplification and sequencing of the 16S rDNA, allowed their affiliation with the genus Bacillus sp. Such a bacterial genus is widely described in the literature as a producer of tensoactives with biosurfactant activities, such as surfactin, due to the presence of the Srf family genes. In this sense, the present study aims for the next period the spectroscopic characterization of biosurfactant and bio-emulsifying compounds produced by Bacillus sp. selected, as well as detecting, by sequence homology, the presence of genes related to surfactin production. In the present study, we verified an amplification of the 675 bp and 201 bp bands, which represent an agreement with the literature of the sfp and srfAA genes, respectively.
- ItemAcesso aberto (Open Access)Bioconversion of low-cost brewery waste to biosurfactant: an improvement of surfactin production by culture medium optimization(Colin Webb, 2021-05-11) Corrêa Nazareth, Talita; Planas Zanutto, Conrado; Maass, Danielle [UNIFESP]; Ulson de Souza, Antônio Augusto; Guelli Ulson de Souza, Selene Maria de Arruda; http://lattes.cnpq.br/3431482044015696Trub is the second-largest residue from brewing industry (250 thousand tons/year) and, due to the presence of high amounts of carbon and nitrogen, it is a potential source of reducing sugars and nitrogen for biological processes. Therefore, this work aimed to enhance surfactin production by B. subtilis using Trub as a carbon source. The effect of nitrogen sources on biosurfactant production was investigated by a Full Factorial Design (2²). Yeast extract and its interaction with peptone presented a statistically significant effect on the response variable of surface tension (ST). ST was described by a linear regression model (R² = 0.93). The effect of metals supplementation on the culture medium was also investigated, where the volumetric productivity increased from 2.98 to 7.43 mg.L-1.h-1 for the medium without (M-I) and with metals (M-II), respectively. After 28h, 210.11 mg.L-1 was obtained in the batch culture with MII medium, being higher than the maximum surfactin concentration achieved after 52 h (121.80 mg.L-1) for M-I medium. The biosurfactant production in bioreactor was successfully performed since a concentrated extract was obtained in the foam collected, reaching the maximum of 1118.20 mg.L-1 of surfactin.
- ItemAcesso aberto (Open Access)Estudo do potencial biotecnológico de fungos endofíticos de manguezais do estado de São Paulo(Universidade Federal de São Paulo, 2016-02-09) Martinho, Vivian [UNIFESP]; Vasconcellos, Suzan Pantaroto de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Discovery of new enzymes acting and assiting the degradation of natural polymers can bring great benefits to the development and/ or growth of second generation ethanol production, as well as for the development of bioremediation processes. It is known that ecological functions of the most microorganisms in nature and their potential applications in biotechnology are still unknown, especially regarding to microorganisms from extreme environments, like mangrove. Among the biotechnological potential of mangroves, it is noteworthy about its community of endophytic fungi, which are considered as effective sources of bioactive compounds, still poorly explored about their versatility. In this sense, the goal of this study was to analyze the enzymatic activity of isolated endophytes from São Paulo State mangroves, checking about their potential for production enzymes for interest in cellulosic ethanol obtention. Their application in bioremediation of fatty waste were also investigated. Thirty two strains of endophytic fungi, morphologically distinct, were evaluated about the presence of ligninase activities, as well as oxygenases, cellulases and xylanases. The production of metabolites with emulsifier and/ or surfactant activity was also investigated. Therefore, among the analyzed isolates, twenty (20) were selected about their ligninolytic activity, twenty-two (22) about oxygenases, eleven (11) about cellulase and fourteen (14) about xylanase. Strains 9(3)2 ? F. sambucinum was selected as higher LiP (72.3 U.L-1) and MnP (29.2 U.L-1) producer. The strain 63.1 ? Fusarium sp. presented proeminent Lac (128.5 U.L-1) activity, while 24(4) ? P. minioluteum showed high oxygenase activity. About cellulolytic and xylanolytic activities, strain 47(4) ? H. lixii showed the highest values: 48.8 and 28.4 U.mL-1, respectively. In addition, fifteen (15) fungi was selected about its bioemulsifier activity, and the strains 94(4) ? Diaporthe sp. and 9(4) ? A. awamori (62.8 %) showed the most elevated performance when automotive motor oil was used as hydrophobic compound
- ItemAcesso aberto (Open Access)Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate(Elsevier, 2021-03-15) Zanutto, Talita Corrêa Nazareth; Zanutto, Conrado Planas; Maass, Danielle [UNIFESP]; Souza, Antônio Augusto Ulson de; Souza, Selene Maria de Arruda Guelli Ulson de; http://lattes.cnpq.br/3431482044015696This work reports surfactin production by B. subtilis ATCC 6051 in a stirred tank bioreactor, where trub was used as a carbon source. Trub is a residue from the brewing industry mainly composed of high-molar-mass proteins and reducing sugars, being a sustainable and low-cost substrate for bioprocess. Despite this, Trub has been little explored as a raw material in bioprocesses. Aiming to evaluate important factors in scale-up process, the impact of oxygen transfer coefficient (KLa) concerning volumetric productivity, and the effect of agitation (150–300 rpm) and aeration (0.5–1.5 vvm) on biosurfactant production by a Full Factorial design were evaluated. Only agitation presented a significant effect on surfactin concentration (SF), where SF was described by a linear regression model (R2 = 0.99). The biosurfactant showed high stability in relation to variations in temperature and pH. In addition, the foam collecting was an efficient method in recovering and enrichment of the product.
- ItemSomente MetadadadosInhibitory effect of a lipopeptide biosurfactant produced by Bacillus subtilis on planktonic and sessile cells of Trichosporon spp.(Taylor & Francis Ltd, 2018) Cordeiro, Rossana de Aguiar; Caracas Cedro, Ewerton Weslley; Colares Andrade, Ana Raquel; Serpa, Rosana; de Jesus Evangelista, Antonio Jose; de Oliveira, Jonathas Sales; Pereira, Vandbergue Santos; Alencar, Lucas Pereira; Leite Mendes, Patricia Bruna; Soares Farias, Barbara Cibelle; Maciel Melo, Vania Maria; de Camargo, Zoilo Pires [UNIFESP]; Collares Maia Castelo-Branco, Debora de Souza; Nogueira Brilhante, Raimunda Samia; Costa Sidrim, Jose Julio; Gadelha Rocha, Marcos FabioThe present study aimed to investigate the inhibitory effect of a bacterial biosurfactant (TIM96) on clinical strains of Trichosporon. Additionally, the effect of TIM96 on the ergosterol content, cell membrane integrity, and the hydrophobicity of planktonic cells was assessed. The inhibitory activity of TIM96 against Trichosporon biofilms was evaluated by analyzing metabolic activity, biomass and morphology. MIC values ranged from 78.125 to 312.5g ml(-1) for TIM96
- ItemAcesso aberto (Open Access)Potencial biorremediador de bactérias isoladas de sedimentos do estuário da Baixada Santista(Universidade Federal de São Paulo, 2017-04-27) Ferrari, Vitor Baptista [UNIFESP]; Vasconcellos, Suzan Pantaroto de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Brazil is considered one of leader countries in the use of sustainable techniques. Among its national territory, it is necessary some special attention to the Brazilian Coastal Zone, mainly because of the intense traffic of ships along it, which can cause oil spills, as well other related environmental accidents. Microorganisms are known about their metabolic characteristics like fast growth, quick acclimation in different environments and high genetic variability. In this context, the present study aimed to investigate the aerobic bacterial diversity of sediments of the Santos estuary (SP, Brazil) regarding to the metabolic activities allowing hydrocarbon biotransformation. From a total of 42 bacterial isolates, twenty-one (21) were selected as potential biodegraders of hexadecane, using a solution of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl- 2 H-tetrazolium bromide 1% (MTT, Merck) as indicator of microbial viability. Analyzes of Gas Chromatography Coupled to Mass Spectrometry (GC-MS) were performed to verify the biodegradation indexes of hexadecane by the selected isolates. Six (6) isolates were detected as able to biodegrade hexadecane, showing indexes above 97%. They were able to degrade 80 % of the hydrocarbon after 48 h of incubation. In addition, Emulsification Indexes (E24) assays allowed the selection of three bacterial strains producing metabolites with bioemulsifying potential, showing E24 values around 50 %. The isolates were characterized by molecular analyzes based on 16S rDNA. They were affiliated to the genus Bacillus sp., Staphylococcus sp., Micrococcus sp., Nitratireductor sp. and Klebsiella sp.
- ItemAcesso aberto (Open Access)Potencial biossurfactante de bactérias isoladas de solos com histórico de contaminação por óleo diesel(Universidade Federal de São Paulo, 2021-08-13) Ferreira, Thais Siqueira [UNIFESP]; Vasconcellos, Suzan Pantaroto de [UNIFESP]; http://lattes.cnpq.br/4254240006926714; http://lattes.cnpq.br/0666150693900757Atualmente, a exploração industrial do petróleo bem como o uso de seus derivados vem crescendo cada vez mais devido à sua importância econômica para a sociedade. Assim, a crescente contaminação dos solos e corpos hídricos por derivados de petróleo tem sido destaque nas últimas décadas. Os biossurfactantes têm chamado a atenção nos últimos anos, devido ao seu potencial à aplicação em processos de biorremediação, são compostos tensoativos produzidos por microrganismos capazes de reduzir a tensão superficial, emulsionar líquidos imiscíveis, degradar hidrocarbonetos e aumentar a mobilidade de contaminantes. Nesse contexto, o presente estudo objetiva a obtenção Substâncias Poliméricas Extracelulares (EPS) de atividade biossurfactante a partir de bactérias isoladas de solos contaminados com óleo diesel, visando aplicação futura em biorremediação. Para isso, as amostras de solo contaminado com diesel foram diluídas e inoculadas, com os isolados obtidos foram realizados os testes dispersão da gota, E24 e tensão superficial para avaliação do potencial biossurfactante. Obteve-se 32 isolados bacterianos, dos quais quatro foram selecionados como produtores de EPS com potencial bio-emulsificante, sendo eles o TTC 04, TTC 12, TTC 20 e TTC 32. Para esses isolados a tensão superficial variou de 33 mN/m (TTC 32) a 43 mN/m (TTC 12). Dessa forma, foi possível selecionar os isolados TTC 32 e TTC 20 com potencial biossurfactante para remediação de sítios contaminados com hidrocarbonetos, sendo que o TTC 32 apresentou 74,24% no índice E24 utilizando óleo de motor usado como agente apolar e reduziu a tensão superficial para 33 mN/m, enquanto o TCC 20 emulsionou o óleo de soja em 62,59% e reduziu a tensão superficial para 35 mN/m Portanto, os microrganismos selecionados produziram compostos biossurfactantes com potencial a aplicações futuras na biorremediação de áreas contaminadas com petróleo e derivados
- ItemSomente MetadadadosThe potential for hydrocarbon biodegradation and production of extracellular polymeric substances by aerobic bacteria isolated from a Brazilian petroleum reservoir(Springer, 2011-06-01) Vasconcellos, S. P. [UNIFESP]; Dellagnezze, B. M.; Wieland, A.; Klock, J. -H.; Santos Neto, E. V.; Marsaioli, A. J.; Oliveira, V. M.; Michaelis, W.; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Campinas (UNICAMP); Univ Hamburg; Petrobras R&D CtrExtracellular polymeric substances (EPS) can contribute to the cellular degradation of hydrocarbons and have a huge potential for application in biotechnological processes, such as bioremediation and microbial enhanced oil recovery (MEOR). Four bacterial strains from a Brazilian petroleum reservoir were investigated for EPS production, emulsification ability and biodegradation activity when hydrocarbons were supplied as substrates for microbial growth. Two strains of Bacillus species had the highest EPS production when phenanthrene and n-octadecane were offered as carbon sources, either individually or in a mixture. While Pseudomonas sp. and Dietzia sp., the other two evaluated strains, had the highest hydrocarbon biodegradation indices, EPS production was not detected. Low EPS production may not necessarily be indicative of an absence of emulsifier activity, as indicated by the results of a surface tension reduction assay and emulsification indices for the strain of Dietzia sp. the combined results gathered in this work suggest that a microbial consortium consisting of bacteria with interdependent metabolisms could thrive in petroleum reservoirs, thus overcoming the limitations imposed on each individual species by the harsh conditions found in such environments.