Navegando por Palavras-chave "Anti-Infective Agents"
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- ItemAcesso aberto (Open Access)Caracterização fenotípica e molecular de amostras de Staphylococcus coagulase negativo isoladas de infecções da corrente sanguínea de pacientes de dois hospitais gerais da cidade de São Paulo(Universidade Federal de São Paulo (UNIFESP), 2009-04-29) Souza, Alinne Guimarães de [UNIFESP]; Pignatari, Antonio Carlos Campos [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Coagulase negative staphylococci (CoNS) are important etiologic agents responsible for healthcare related infection (HCRI) in bloodstream infections. In a retrospective study we evaluate CoNS isolated from patients with HCRI in bloodstream infections admitted to two general hospitals at São Paulo city, from August 2005 to August 2007. The isolates were characterized at species level and antimicrobial susceptibility tested by the Vitek® system and ViteK® 2. Oxacillin resistance was evaluated by oxacillin and cefoxitin discs. The presence of mecA gene and the SCCmec type were determined by multiplex PCR. Staphylococcus epidermidis was the predominant specie followed by S. hominis, S. haemolyticus, S. simulans, S. warneri, S. capitis and S. auricularis. Oxacillin resistance was observed in 88.2% of the isolates with 100% concordance between oxacilin and cefoxitin discs confirmed by mecA gene. All isolates were susceptible to vancomycin and four isolates revealed intermediate resistance to teicoplanin by Etest®. One S. epidermidis showed resistance to linezolide. The predominant SCC type was the type III followed by the types IV, I, II and V. In 26.9% positive mecA isolates we did not characterize the SCCmec type by the molecular protocol utilized.
- ItemAcesso aberto (Open Access)Evaluation of antimicrobial and antitumoral activity of Garcinia mangostana L. (mangosteen) grown in Southeast Brazil(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-01-01) Cunha, Bruna Lais Almeida; França, Jerônimo Pereira de [UNIFESP]; Moraes, Andrea Aparecida Fátima Souza [UNIFESP]; Chaves, Alba Lucilvânia Fonseca; Gaiba, Silvana; Fontana, Renato; Sacramento, Celio Kersul do; Ferreira, Lydia Masako [UNIFESP]; França, Lucimar Pereira de; Universidade Estadual de Santa Cruz Department of Biological Sciences; Universidade Estadual de Santa Cruz Departament of Agricultural and Environmental Sciences; Universidade Federal de São Paulo (UNIFESP)PURPOSE:To characterize the anatomy of the fruit and leaf and the presence of phytocompounds. To evaluate the antitumor and antimicrobial activity of ethanolic extract of Garcinia mangostana L. (mangosteen) cultivated in southeastern Brazil.METHODS:Anatomical characterization and histochemical reactions were performed for structural identification and the presence of phytocompounds. Preparation of ethanolic extract of the fruit, leaf and resin of mangosteen. Culture B16-F10 melanoma cells for treatment with mangosteen ethanolic extract to determine cell viability by MTT and genotoxic effect by comet assay. Evaluation by antimicrobial activity against Staphylococcus aureus and Escherichia coli by agar diffusion test and by determination of Minimum Inhibitory Concentration (MIC).RESULTS:Our results showed many secretory canals in resin fruit and leaf; identifying lipids, starch, lignin and phenolic compounds. The leaf extract induced genotoxicity and apoptosis in B16-F10 cells, since the fragmentation of DNA in the comet assay. The ethanolic extract of mangosteen obtained in the resin, leaf and fruit showed antimicrobial activity against Staphylococcus aureus and Escherichia coli with a MIC at 0.1 mg/mL.CONCLUSION: In conclusion, we have demonstrated both antimicrobial and antitumor activity of ethanol extract of mangosteen emphasizing its therapeutic potential in infectious diseases and in cancer, such as melanoma.
- ItemRestritoEvaluation of antitumoral and antimicrobial activity of Morinda lcitrifolia L. grown in Southeast Brazil(Acta Cirurgica Brasileira, 2014-01-01) Candida, Thamyris; Franca, Jeronimo Pereira de; Fonseca Chaves, Alba Lucilvania; Rodrigues Lopes, Fernanda Andrade; Gaiba, Silvana; Sacramento, Celio Kersul do; Ferreira, Lydia Masako [UNIFESP]; Franca, Lucimar Pereira de; Universidade Estadual Santa Cruz; Universidade Estadual Santa Cruz; Universidade Federal de São Paulo (UNIFESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico; Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorPURPOSE: To evaluate the antitumor and antimicrobial activity of ethanolic extract of Morinda citrifolia L. fruit cultivated in southeastern Brazil.METHODS: Preparation ethanolic extract of the fruit of Morinda citrifolia L. Culture of melanoma cells B16-F10 for treatment with ethanolic extract of Morinda citrifolia L. fruit to determine cell viability by MTT and determination temporal effect of ethanolic extract fruit on the cell growth B16-F10 for 8 days. Evaluation of antimicrobial activity of ethanolic extract fruit against Staphylococcus aureus and Escherichia coli by determination of Minimum Inhibitory Concentration (MIC).RESULTS: the ethanolic extract of Morinda citrifolia L. fruit (10mg/mL) decreased cellular activity and inhibited 45% the rate of cell proliferation of B16-F10 melanoma treated during period studied. the ethanolic extract of Morinda citrifolia L. fruit demonstrated antimicrobial activity inhibiting the growth of both microorganisms studied. Staphylococcus aureus was less resistant to ethanolic extract of Morinda citrifolia L. fruit than Escherichia coli, 1 mg/mL and 10 mg/mL, respectively.CONCLUSION: What these results indicate that the ethanolic extract of the fruit of Morinda citrifolia L. showed antitumor activity with inhibition of viability and growth of B16-F10 cells and also showed antibacterial activity as induced inhibition of growth of Staphylococcus aureus and Escherichia coli.