Navegando por Palavras-chave "Adult Stem Cells"
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- ItemAcesso aberto (Open Access)Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-01-01) Suartz, Caio Vinicius; Gaiba, Silvana; França, Jerônimo Pereira de; Aloise, Antonio Carlos [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Santa Cruz Department of SurgeryPURPOSE:To evaluate the viability of random pattern dorsal skin flaps in rats after injection of adipose-derived stem cells (ADSC).METHODS: Thirty five adult male Wistar EPM rats (weight 250-300 g) were distributed, at random, in two groups. I- Control (flap elevation with injection of saline solution) with fifteen animals and II- Experimental (flap elevation with injection of ADSC ) with fifteen animal. The ADSC were isolated from others five adult male rats. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed in both groups and the injection (cells or saline solution) were perfomed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS:The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation to verify their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSIONS:The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random random pattern dorsal skin flaps in rats.
- ItemAcesso aberto (Open Access)Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-01-01) Suartz, Caio Vinicius [UNIFESP]; Gaiba, Silvana; França, Jerônimo Pereira de; Aloise, Antonio Carlos [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Santa Cruz Department of Biological SciencesPURPOSE:To evaluate the effects of the adipose-derived stem cells (ADSC) in the viability of random skin flap in rats.METHODS:Thirty five adult male Wistar rats (weight 250-300 g) were used. ADSC were isolated from adult male rats (n=5). ADSC were separated, cultured and then analyzed. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surgical procedure, the animals were randomized into two groups (n=15 each group), group control and group ADSC. In all groups the procedures were performed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS:The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSION: The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random skin flaps in rats.
- ItemAcesso aberto (Open Access)Aldefluor protocol to sort keratinocytes stem cells from skin(Acta Cirurgica Brasileira, 2017) Ribeiro Noronha, Samuel Marcos [UNIFESP]; Gragnani, Alfredo [UNIFESP]; Calado Pereira, Thiago Antonio [UNIFESP]; Alves Correa, Silvana Aparecida [UNIFESP]; Bonucci, Jessica [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]Purpose: To investigate the use Aldefluor r and N, N-Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. Methods: Activated Aldefluor r aliquots were prepared and maintained at temperature between 2 to 8 degrees C, or stored at -20 degrees C. Next, the cells were collected following the standard protocol of sample preparation. Results: Best results were obtained with Aldefluor r 1.5 mu l and DEAB 15 mu l for 1 x 10(6) cells, incubated at 37 degrees C for 15 minutes. Flow cytometer range for keratinocyte stem cells separation was evaluated. There were 14.8% of stem cells separated in one sample of keratinocyte culture used to pattern the protocol. After being defined the ideal concentration, the same test pattern was performed in other keratinocyte samples. We observed a final mean of 10.8%. Conclusion: Aldefluor r has been shown as a favorable marking of epidermal keratinocyte stem cells for subsequent separation on a flow cytometer, with detection of 10.8% of epidermal keratinocyte stem cells, in this protocol.
- ItemAcesso aberto (Open Access)Characterization of human adipose-derived stem cells(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2012-07-01) Gaiba, Silvana; França, Lucimar Pereira De; França, Jerônimo Pereira De; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: There is a growing scientific interest in the plasticity and therapeutic potential of adipose-derived stem cells (ASCs), which are multipotent and abundant in adipose tissue and can differentiate in vitro into multiple lineages, including adipocytes, chondrocytes, osteoblasts, neural cells, endothelial cells and cardiomyocytes. The aim of this study was to isolate, cultivate and identify ASCs. METHODS: Human adipose precursor cells were obtained from subcutaneous abdominal tissue. Recently dispersed cells were separated by density centrifugation gradient, cultured and then analyzed. RESULTS: Human ASCs were able to replicate in our culture conditions. The cells maintained their phenotypes throughout the studied period on different passages confirming they suitability for in vitro cultivation. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro. Flow cytometry results showed that these cells expressed CD73, CD90 and CD105, (mesenchymal stem-cells markers), contrasting with the lack of expression of CD16, CD34 and CD45 (hematopoietic cells markers). CONCLUSION: It was possible to isolate human adipose-derived stem cells by in vitro cultivation without adipogenic induction, maintaining their functional integrity and high proliferation levels. The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro.
- ItemAcesso aberto (Open Access)Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2009-10-01) Eça, Lilian Piñero Marcolin; Ramalho, Renata Belmonte; Oliveira, Isis Sousa [UNIFESP]; Gomes, Paulo Oliveira [UNIFESP]; Pontes, Paulo [UNIFESP]; Ferreira, Alice Teixeira [UNIFESP]; Mazzetti, Marcelo Paulo Vaccari [UNIFESP]; Stem Cell Research Institute; Sacred Heart University; Universidade Federal de São Paulo (UNIFESP); Lusiada University of Santos Department Plastic SurgeryPURPOSE: To assess the technique for the collection of rabbit bone marrow stem cells from different regions to be used as an experimental model in regenerative medicine. METHODS: Thirty rabbits were allocated into 2 groups: GROUP A, n=8, animals that underwent bone marrow blood (BMB) harvesting from the iliac crest; and GROUP B: including 22 rabbits that underwent BMB harvesting from the femur epiphysis. After harvesting, mononuclear cells were isolated by density gradient centrifugation (Ficoll - Histopaque). The number of mononuclear cells per ml was counted in a Neubauer chamber and cell viability was checked through Tripan Blue method. RESULTS: Harvesting from the iliac crest yielded an average of 1 ml of BMB and 3,6.10(6) cells/ml over 1 hour of surgery, whereas an average of 3ml of BMB and 11,79.10(6) cells./ml were obtained in 30 min from the femur epiphysis with a reduced animal death rate. CONCLUSION: The analysis for the obtention of a larger number of mononuclear cells/ml from rabbit bone marrow blood was more satisfactory in the femur epiphysis than in the iliac crest.