Navegando por Palavras-chave "ANA"

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    From autoantibody research to standardized diagnostic assays in the management of human diseases - report of the 12th Dresden Symposium on Autoantibodies
    (Sage Publications Ltd, 2016) Conrad, K.; Andrade, L. E. C. [UNIFESP]; Chan, E. K. L.; Mahler, M.; Meroni, P. L.; Pruijn, G. J. M.; Steiner, G.; Shoenfeld, Y.
    Testing for autoantibodies (AABs) is becoming more and more relevant, not only for diagnosing autoimmune diseases (AIDs) but also for the differentiation of defined AID subtypes with different clinical manifestations, course and prognosis as well as the very early diagnosis for adequate management in the context of personalized medicine. A major challenge to improve diagnostic accuracy is to harmonize or even standardize AAB analyses. This review presents the results of the 12th Dresden Symposium on Autoantibodies that focused on several aspects of improving autoimmune diagnostics. Topics that are addressed include the International Consensus on ANA Patterns (ICAP) and the International Autoantibody Standardization (IAS) initiatives, the optimization of diagnostic algorithms, the description and evaluation of novel disease-specific AABs as well as the development and introduction of novel assays into routine diagnostics. This review also highlights important developments of recent years, most notably the improvement in diagnosing and predicting the course of rheumatoid arthritis, systemic sclerosis, idiopathic inflammatory myopathies, and of autoimmune neurological, gastrointestinal and liver diseases
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    I Consenso Nacional para Padronização dos Laudos de FAN HEp-2
    (Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia, 2002-07-01) Dellavance, Alessandra [UNIFESP]; Gabriel Júnior, Alexandre [UNIFESP]; Cintra, Alice Friedenberg de Ulhôa; Ximenes, Antônio Carlos; Nuccitelli, Barbara; Mühlen, Carlos Alberto Von; Bichara, Carlos David Araújo; Yano, Cristiane; Carvalho, Darlene Gonçalves; Bonfá, Eloisa Silva Dutra de Oliveira; Guimarães, Fabiana N.c.; Mundim, Hugo M.; Pfrimer, Irmtraut Araci Hoffmann; Rego, Jozelia; Andrade, Luiz Eduardo Coelho [UNIFESP]; Mesquita, Mauro Meira de; Santiago, Mittermayer Barreto; Silva, Nilzio Antonio; Miranda, Paulo J.; Leser, Paulo; Francescantonio, Paulo Luiz Carvalho; Jarach, Renata; Levy, Roger Abramino; Neves, Suzane Pretti Figueiredo; Cruvinel, Wilson de Melo [UNIFESP]; Santos, Wilton Silva dos [UNIFESP]; Laboratório Fleury; Centro Imuno Reumatológico de São Paulo; Biorad Diagnósticos; Universidade Federal de Goiás; Laboratório Clínico Padrão; Pontifícia Universidade Católica; Laboratório Amaral Costa; Universidade Católica de Goiás; Laboratório Pardini; Universidade de São Paulo (USP); Laboratório Exame; Universidade Federal de São Paulo (UNIFESP); EBMSP; Universidade Federal de Pernambuco; UERJ; Laboratório Labs; UFMG; Hospital de Base
    The technique of immunofluorescence using HEp-2 cells as substrate is the screening method of choice for the presence of autoantibodies in many clinical laboratories. The lack of a specific terminology for reporting results brings problems in quality control, clinical utility of the test, and standardization attempts. The first Brazilian Consensus for Standardization of ANA in HEp-2 Cells took place in Goiânia in August 2000. Several laboratory specialists with experience in the methodology showed up. They established guidelines for the description of ANA patterns in the Portuguese language, encompassing distinct descriptions for nuclear, nucleolar, cytoplasmic and mitotic apparatus patterns of fluorescence. Recommendations were also established regarding screening titers, final dilution titer, and on morphological criteria for reading the slides.
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    II Consenso Brasileiro de Fator Antinuclear em Células HEp-2: Definitions for standardization of autoantibody testing against the nucleus (ANA HEp-2), nucleolus, cytoplasm and mitotic apparatus, as wel as its clinical associations
    (Sociedade Brasileira de Reumatologia, 2003-06-01) Dellavance, Alessandra [UNIFESP]; Gabriel Júnior, Alexandre [UNIFESP]; Cintra, Alice Friedenberg de Ulhôa; Ximenes, Antônio Carlos; Nuccitelli, Barbara; Taliberti, Ben Hur; Moreira, Caio; Von Mühlen, Carlos Alberto; Bichara, Carlos David Araújo; Santos, Cláudio Henrique Ramos dos; Yano, Cristiane Martinez; Mangueira, Cristóvão Luis Pitangueiras; Carvalho, Darlene Gonçalves; Bonfá, Eloisa Silva Dutra de Oliveira; Doi, Elvira M.; Guimarães, Fabiana Nunes de Carvalho; Araújo, Flávia Ikeda e; Mundim, Hugo Mendonça; Rego, Jozelia; Vieira, Lisiane Ericonio dos Anjos; Poli, Luciana; Andrade, Luiz Eduardo Coelho [UNIFESP]; Callado, Maria Roseli; Mesquita, Mauro Meira; Sugiyama, Mitiko; Slhessarenko, Natasha; Silva, Nilzio Antônio da; Carballo, Orlando Gabriel; Leser, Paulo Guilherme [UNIFESP]; Francescantonio, Paulo Luiz Carvalho; Jarach, Renata; Xavier, Ricardo Machado; Levy, Roger Abramino; Neves, Suzane Pretti Figueiredo; Cruvinel, Wilson de Melo [UNIFESP]; Santos, Wilton Silva dos [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Bio-Rad Laboratório Brasil; Hospital Geral de Goiânia; Biomédica; Universidade Federal de Uberlândia; UFMG HC; PUCRS HC; New Life Produtos Hospitalares; Universidade Católica de Goiás; Patologista Clínica; Frischmann Aisengart Unidad Inmunología; Universidade Católica de Goiás Laboratório de Auto-Imunidade; Exame Medicina Laboratorial; FMUFG HC Laboratório de Imuno-Reumatologia e HLA; Lab. Santa Luzia; Medivax/Bion; HSPE/SP; Universidade Católica de Goiás Laboratório da Área de Saúde; Farmacêutica-Bioquímica; Univ. Fed. Mato Grosso; FMUFG Serviço de Reumatologia; Hospital Durand Unidad Inmunología; Laboratório Clínico; UFRGS HCPA Serviço de Reumatologia; UERJ FCM; UFMG FM; Hospital Universitário de Brasília Laboratório de Reumatologia
    OBJECTIVE: The Second Brazilian Consensus on Antinuclear Antibodies (ANA) in HEp-2 Cells approved and extended the decision trees developed during the First Brazilian Consensus in order to also offer information about mixed patterns of fluorescence. METHODS: Since this test elicits reactions not only to nuclear autoimmune antigens but also to different cell compartments, new denominations for the test were approved. Results and CONCLUSIONS: These new denominations encompass variations on the autoantibody testing against the nucleus (ANA HEp-2), nucleolus, cytoplasm, and mitotic apparatus issue. Furthermore, major clinical associations were described for each immunofluorescent pattern, facilitating the interpretation of laboratory results in the clinical practice.