Navegando por Palavras-chave "murine model"

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    Alternatives to amphotericin B for Candida rugosa infection
    (Oxford Univ Press, 2004-08-01) Hernandez, S.; Gonzalez, G. M.; McCarthy, D. I.; Colombo, Arnaldo Lopes [UNIFESP]; Najvar, L. K.; Bocanegera, R.; Graybill, JR; Univ Texas; Univ Autonoma Nuevo Leon; Universidade Federal de São Paulo (UNIFESP)
    Objective: Amphotericin B failure is frequently seen in patients with candidaemia caused by Candida rugosa. We evaluated amphotericin 13, fluconazole, posaconazole and voriconazole as alternative treatments against infection in mice with two isolates of C. rugosa.Methods: Neutropenic mice were inoculated intravenously with C. rugosa. Amphotericin 13, fluconazole, posaconazole and voriconazole were administered for 7 days after infection. Efficacy of the antifungal treatment was assessed by survival and tissue burden of C. rugosa.Results: All of the four drugs significantly prolonged survival over controls. With both isolates, kidney counts were reduced significantly below controls for amphotericin B, fluconazole and posaconazole. However, voriconazole was less effective than the other antifungals.Conclusion: Despite poor clinical response to amphotericin B, in vivo data indicate that amphotericin B increases organ clearance and survival over untreated controls. However, although voriconazole improved survival over controls, increased tissue clearance was not seen. This discrepancy may be caused by rapid clearance of voriconazole in mice. These studies suggest fluconazole, posaconazole or voriconazole may be useful alternatives to amphotericin B in therapy of C. rugosa infection.
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    Cutaneous murine model of infection caused by Neoscytalidium dimidiatum: a preliminary study of an emerging human pathogen
    (Oxford Univ Press, 2016) da Silva, Roberta Teixeira; Guimaraes, Davson A.; Camargo, Zoilo Pires de [UNIFESP]; Rodrigues, Anderson Messias [UNIFESP]; Maceira, Juan P.; Bernardes-Engemann, Andrea R.; Orofino-Costa, Rosane
    Neoscytalidium dimidiatum is an emerging fungus that causes a skin infection similar to dermatophytosis
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    Study of acute chagasic mice under immunosuppressive therapy by cyclosporin A: modulation and confocal analysis of inflammatory reaction
    (Elsevier B.V., 2000-04-01) Calabrese, K. S.; Paradela, ASRC; Valle, T. Z. do; Tedesco, R. C.; Silva, S.; Mortara, R. A.; Costa, SCG da; Ist Oswaldo Cruz; Universidade Federal de São Paulo (UNIFESP)
    The in vivo effects of cyclosporin A (CsA) on Trypanosoma cruzi infection were examined using different schedules of the drug in mice infected with the Y strain. Parasitaemia at day 8 after infection among CsA-treated animals was usually higher than control infected non-treated mice. On the other hand, mortality analysis showed that animals CsA-treated either with 200 mg/kg 2 days before infection or with therapeutic doses (10 mg/kg every other day) showed almost the same mean time of death (35.8 and 38.2 days, respectively). in these groups mice died 50% less than control infected non-treated ones. the mean time of death in the animals treated with 200 mg/kg 5 days after infection and in infected non-treated control mice were respectively 29.0 and 22.6 days. the kinetics analysis of the leukocyte population of animals treated with a single dose of 200 mg/kg of CsA before or after infection did not show the alternate pattern of leukopenia/leukocytosis observed in control groups of infected mice but differential cell counts indicated a modulatory action upon circulating leukocytes of therapeutic doses of CsA. the animals treated with any of the CsA schedules showed a moderate to intense diffuse inflammatory reaction exhibiting mainly mononuclear cells in the heart. Immunofluorescence analysis by confocal microscopy revealed that macrophages are a major component of the inflammatory infiltrate in all groups of CsA-treated mice and also in the control group. (C) 2000 Elsevier Science B.V. All rights reserved.