Navegando por Palavras-chave "lúpus eritematoso sistémico"

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    Neutropenia autoimune secundária em portadores de lupus eritematoso sistêmico
    (Universidade Federal de São Paulo (UNIFESP), 2013-01-30) Cruz, Lafayette Cavalcanti Bezerra Dias [UNIFESP]; Bordin, Jose Orlando Bordin [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Systemic Lupus Erythematosus (SLE) is a systemic inflammatory disease with multiple organ involvement. Hematological changes in the criteria reviewed include hemolytic anemia, leukopenia, lymphopenia and thrombocytopenia, and no report of neutropenia. It is understood that neutropenia in SLE may occur as a result of various etiologies, like medications, bone marrow dysfunction and autoimmune reaction. Objectives: 1) Identify, in SLE patients with neutropenia, the presence of neutrophilic autoantibodies. 2) Determine the population profile in this sample of patients. Patients and Methods: we determined the presence of neutrophilic autoantibodies in serum samples of 20 patients with SLE, selected by neutropenia <1600 cells/mm3, from the rheumatology clinic in HSP / UNIFESP. We used the immunofluorescence granulocyte (GIFT) technique by flow cytometry and fluorescence microscopy after isolation of mononuclear cells and granulocytes from blood cells by sedimentation to 5% Dextran, followed by centrifugation on Ficoll-Paque (d = 1.077, Pharmacia), with viability greater than 80% by reaction with Trypan Blue. Incubated with antiserum (anti-HNA1a,-1b, -1b, -2a, -3a e -4a, obtained from the American Red Cross, North Central Blood Services, St. Paul, MN). In a later step, we used granulocyte from male-blood group-AB volunteer donors with serum of patients in research, with the same prior technique, identifying autoantibodies. All patients also underwent collection of bone marrow, serum protein electrophoresis, immunoglobulin levels and serology for hepatitis B, hepatitis C, HIV and cytomegalovirus. Results: All cases were female, with normal myelogram, negative serology, without hypogammaglobulinemia. Neutrophilic autoantibodies were present in 60% of samples, with 40% remaining showing viability below the cut-off limit for the study. Conclusions: This study reveals the presence of neutrophilic autoantibodies as the cause of neutropenia in patients with Systemic Lupus Erythematosus. In spite of the lack of other similar researches, we found in this work autoantibodies HNA1a and HNA2a, granulocyte antigens of greater frequency in the population.