Navegando por Palavras-chave "Wistar"

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    Comparative mRNA and MicroRNA Profiling during Acute Myocardial Infarction Induced by Coronary Occlusion and Ablation Radio-Frequency Currents
    (Frontiers Media Sa, 2016) Santana, Eduardo T.; Feliciano, Regiane dos Santos; Serra, Andrey J.; Brigidio, Eduardo; Antonio, Ednei Luiz [UNIFESP]; Tucci, Paulo José Ferreira [UNIFESP]; Nathanson, Lubov; Morris, Mariana; Silva, Jose A., Jr.
    The ligation of the left anterior descending coronary artery is the most commonly used experimental model to induce myocardial infarction (MI) in rodents. A high mortality in the acute phase and the heterogeneity of the size of the MI obtained are drawbacks recognized in this model. In an attempt to solve the problem, our group recently developed a new MI experimental model which is based on application of myocardial ablation radio-frequency currents (AB-RF) that yielded MI with homogeneous sizes and significantly reduce acute mortality. In addition, cardiac structural, and functional changes aroused by AB-RF were similar to those seen in animals with MI induced by coronary artery ligation. Herein, we compared mRNA expression of genes that govern post-MI milieu in occlusion and ablation models. We analyzed 48 mRNAs expressions of nine different signal transduction pathways (cell survival and metabolism signs, matrix extracellular, cell cycle, oxidative stress, apoptosis, calcium signaling, hypertrophy markers, angiogenesis, and inflammation) in rat left ventricle 1 week after MI generated by both coronary occlusion and AB-RF. Furthermore, high-throughput miRNA analysis was also assessed in both MI procedures. Interestingly, mRNA expression levels and miRNA expressions showed strong similarities between both models after MI, with few specificities in each model, activating similar signal transduction pathways. To our knowledge, this is the first comparison of genomic alterations of mRNA and miRNA contents after two different MI procedures and identifies key signaling regulators modulating the pathophysiology of these two models that might culminate in heart failure. Furthermore, these analyses may contribute with the current knowledge concerning transcriptional and post-transcriptional changes of AB-RF protocol, arising as an alternative and effective MI method that reproduces most changes seem in coronary occlusion.
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    Comparative study of the effects of low-intensity pulsed ultra-sound and low level laser therapy on injured muscle repair
    (Spie-int Soc Optical Engineering, 2011-01-01) Renno, Ana Claudia Muniz [UNIFESP]; Toma, Renata Luri [UNIFESP]; Feitosa, Suellen Maurin [UNIFESP]; Fernandes, Kelly Rossetti [UNIFESP]; Oliveira, Poliani de [UNIFESP]; Parizotto, Nivaldo Antonio [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Hamblin, Michael R.; Waynant, R. W.; Anders, J.; Universidade Federal de São Paulo (UNIFESP)
    Muscle tissue is one of the most frequently affected by injury, whether during sports activities, or work activities. in this context, biochemical and biophysical resources have been studied to minimize the time of muscle regeneration. Among these, low intensity pulsed ultrasound (US) and low level laser therapy (LLLT) may be highlighted. Despite a series of evidences about the positive effects of these resources in the process of tissue regeneration, the cellular and morphological changes triggered by LLLT and U. S. are still largely unknown. Thus, the aim of this study was to investigate the effects of US and LLLT on muscle repair after cryolesion by means of histopathological analysis and immunohistochemistry for COX-2. A total of thirty five male Wistar rats were randomly distributed into 4 groups: intact control group; injured control group: muscle injured animals without any treatment; laser treated group: muscle injured animals treated with 830 nm laser and ultra-sound treated group: muscle injured animals treated with US. the treatments started 24 hours post-surgery and were performed during 6 sessions. the animals exposed to lasertherapy pointed out minor degenerative changes of muscle tissue. in the same way, exposure to ultrasound was able to reduce tissue injuries induced by cryolesion, but less intense than laser therapy. Strong COX-2 positive cells were found in rats submitted to cryolesion only, whereas COX-2 immunoexpression was lower in laser treated or ultrasound treated groups. in summary, this study reveals that both lasertherapy and ultrasound have positive effects on muscle repair in rats.
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    Efeito da leucina na hipertrofia cardíaca e na concentração sérica de creatina quinase em ratos Wistar em hipertireoidismo experimental
    (Univ Federal Uberlandia, 2013-03-01) Fidale, Thiago Montes; Goncalves, Alexandre; Paranhos Lopes, Leandro Teixeira; Alves, Poliana Rodrigues; Souza, Fernanda Rodrigues de; Dias Nunes, Joao Elias; Mundim, Antonio Vicente; Resende, Elmiro Santos [UNIFESP]; Universidade Federal de Uberlândia (UFU); Universidade de Brasília (UnB); Universidade Federal de São Paulo (UNIFESP)
    Leucine is a regulator of protein metabolism in vivo, and there is little information regarding its action on cardiac hypertrophy induced by experimental hyperthyroidism and its relationship to serum creatine kinase. The study aimed to verify the effect of leucine in cardiac hypertrophy and serum creatine kinase in rats with hyperthyroidism. We used 20 animals were divided into four groups and these control, hormone, hormone + leucine and leucine. Hyperthyroidism was induced by administration of daily 20 mu g/100 grams of levothyroxine sodium in aqueous suspension by gavage. Leucine was supplemented by adding 5% of the amino conventional diet. Blood was collected by cardiac puncture and analyzes made in kit CK-NAC, CK-MB and TSH. At the end of the trial period of seven days the hearts were removed and weighed. Subsequently, the left ventricle was separated together with the interventricular septum and weighed. In statistical comparison was used analysis of variance (ANOVA) and two-way post-Tukey test, considering p values <0.05. Serum concentrations of serum CK-MB were lower in leucine (p <0.05) and hormone + leucine (p <0.05) compared to control. The hormone group, showed greater relative heart weight than the control group (p <0.05) and absolute heart weight greater than hormone + leucine (p <0.05). In conclusion, leucine appears to reduce cardiac hypertrophy and serum concentration of creatine kinase by unknown mechanisms.
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    Effects of different fluences of low-level laser therapy in an experimental model of spinal cord injury in rats
    (Springer London Ltd, 2017) Veronez, Suellen de Oliveira [UNIFESP]; Assis, Livia [UNIFESP]; Del Campo, Paula [UNIFESP]; De Oliveira, Flavia [UNIFESP]; Castro, Glaucia Monteiro de [UNIFESP]; Renno, Ana Claudia Muniz [UNIFESP]; Medalha, Carla Christina [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The aim of this study was to evaluate the in vivo response of different fluences of low-level laser therapy (LLLT) on the area of the injury, inflammatory markers, and functional recovery using an experimental model of traumatic spinal cord injury (SCI). Thirty two rats were randomly divided into four experimental groups: control group (CG), laser-treated group 500 J/cm(2) (L-500), laser-treated group 750 J/cm(2) (L-750), and laser-treated group 1000 J/cm(2) (L-1000). SCI was performed by an impactor equipment (between the ninth and tenth thoracic vertebrae), with a pressure of 150 kdyn. Afterwards, the injured region was irradiated daily for seven consecutive sessions, using an 808-nm laser, at the respective fluence of each experimental groups. Motor function and tactile sensitivity were performed on days 1 and 7 post-surgery. Animals were euthanized on the eighth day after injury, and the samples were retrieved for histological and immunohistochemistry analyses. Functional evaluation and tactile sensitivity were improved after LLLT, at the higher fluence. Additionally, LLLT, at 750 and 1000 J/cm(2), reduces the lesion volume and modulates the inflammatory process with decrease of CD-68 protein expression. These results suggest that LLLT at higher doses was effective in promoting functional recovery and modulating inflammatory process in the spinal cord of rats after SCI.
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    Potencial inflamatório induzido pelo crack em fígado de ratos wistar
    (Universidade Federal de São Paulo, 2024-06-25) Caires, Jéssica Carine da Costa [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Souza, Daniel Vitor de [UNIFESP]; http://lattes.cnpq.br/7955161915979529; http://lattes.cnpq.br/9969803499258672; http://lattes.cnpq.br/6713833111906386; Universidade Federal de São Paulo (UNIFESP)
    O crack é um subproduto originário da adição de bicarbonato de sódio à pasta base de cocaína, cuja apresentação é sob a forma de cristais irregulares (“pedras"), de coloração marrom e/ou bege amarelado. A utilização e a disseminação desenfreada do crack pelo país vem sendo encarados como um grande problema social e de saúde pública. Pesquisas recentes apontaram o Brasil como o maior mercado consumidor de crack no mundo. Entretanto, os efeitos tóxicos em tecidos ou órgãos induzidos pelo crack sob a forma inalatória ainda não estão totalmente elucidados. Dessa forma, o objetivo da presente pesquisa foi avaliar o potencial inflamatório e citotóxico induzido pelo crack em tecido hepático em modelo experimental in vivo. Para tal, a pesquisa simulou uma exposição de crack por via inalatória em ratos Wistar machos, no qual os animais foram distribuídos, em 4 grupos (n=6): 0, 25, 50 e 100 mg/kg de crack por 5 dias consecutivos, uma vez ao dia. Após esse período, o tecido hepático foi investigado por meio de avaliação histopatológica. O estudo concluiu que, ao final do período, todas as doses foram capazes de gerar danos no tecido hepático, entretanto, apenas a exposição a maior dose apresentou diferença estatística (p < 0,05). Contudo, não foi encontrada a presença de infiltrado inflamatório.
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    Repercussões da interposição ileal isolada em ratos com dismetabolismo glicídico induzido por dieta
    (Universidade Federal de São Paulo (UNIFESP), 2017-07-31) Leal, Lucas Pedroso Fernandes Ferreira [UNIFESP]; Azevedo, João Luiz Moreira Coutinho de [UNIFESP]; Lapchik, Milton Soibelmann [UNIFESP]; Gagliardi, Antonio Ricardo de Toledo; Milton Soibelmann Lapchik: http://lattes.cnpq.br/7871854683321407; Antonio Ricardo de Toledo Gagliardi: http://lattes.cnpq.br/3795666743699103; http://lattes.cnpq.br/2507285909783854; http://lattes.cnpq.br/4910203611295452; Universidade Federal de São Paulo (UNIFESP)
    INTRODUCTION: The clinical management of type 2 diabetes mellitus (T2DM) is complex and permanent. In this moment, it is necessary a surgical procedure considered effective for the treatment of this disease among individuals with body mass index above morbid obesity. The isolated ileal transposition (III) could become an alternative therapy in selected non-obese diabetic patients. There are no reports of III performed in wistar rats in an experimental model of diet-induced disglycemia without genetic or drug influences, nor its pancreas morphology evaluation. OBJECTIVE: This study aims to contribute to the treatment of T2DM, evaluating the physiological effects of III on glucose metabolism and investigating histological changes of the pancreas in an experimental model of diet-induced disglycemia. METHODS: Seventy two 12-week-old male Wistar rats were distributed into four groups - ileal transposition, sham-operated, diabetic controls and non-diabetic controls (NG). The NG group received usual rat feeding while the other groups received hypercaloric-hyperlipidic diets. III was carried out in obese disglycemic rats at the 30-week. All groups maintained their diets untill the 48-week. Glucose tolerance, insulin resistance, food-intake, body weight, adiposity, serum substances and pancreas morphology were evaluated. RESULTS: After a postoperative period of 18 weeks, the III improved glucose tolerance and insulin sensitivity in diet-induced disglycemic rats. III did not induced weight loss or food intake decrease, and did not affected fat mass. It did not promoted morphological pancreatic alterations. CONCLUSIONS: In rats with diet-induced disglycemia, III promotes beneficial changes in glucose metabolism and does not modify the morphology of the endocrine pancreas.