Navegando por Palavras-chave "Trub"
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- ItemAcesso aberto (Open Access)Bioconversion of low-cost brewery waste to biosurfactant: an improvement of surfactin production by culture medium optimization(Colin Webb, 2021-05-11) Corrêa Nazareth, Talita; Planas Zanutto, Conrado; Maass, Danielle [UNIFESP]; Ulson de Souza, Antônio Augusto; Guelli Ulson de Souza, Selene Maria de Arruda; http://lattes.cnpq.br/3431482044015696Trub is the second-largest residue from brewing industry (250 thousand tons/year) and, due to the presence of high amounts of carbon and nitrogen, it is a potential source of reducing sugars and nitrogen for biological processes. Therefore, this work aimed to enhance surfactin production by B. subtilis using Trub as a carbon source. The effect of nitrogen sources on biosurfactant production was investigated by a Full Factorial Design (2²). Yeast extract and its interaction with peptone presented a statistically significant effect on the response variable of surface tension (ST). ST was described by a linear regression model (R² = 0.93). The effect of metals supplementation on the culture medium was also investigated, where the volumetric productivity increased from 2.98 to 7.43 mg.L-1.h-1 for the medium without (M-I) and with metals (M-II), respectively. After 28h, 210.11 mg.L-1 was obtained in the batch culture with MII medium, being higher than the maximum surfactin concentration achieved after 52 h (121.80 mg.L-1) for M-I medium. The biosurfactant production in bioreactor was successfully performed since a concentrated extract was obtained in the foam collected, reaching the maximum of 1118.20 mg.L-1 of surfactin.