Navegando por Palavras-chave "Transforming Growth Factor Beta"

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    Expressão proteica de BMP-7, MMP-9 e TGF-β e suas correlações com as interleucinas 6 e 10 nas rinossinusites crônicas
    (Universidade Federal de São Paulo (UNIFESP), 2020-08-27) Lucas, Bernardo Relvas [UNIFESP]; Pezato, Rogerio [UNIFESP]; Universidade Federal de São Paulo
    Introduction: Chronic Rhinosinusitis has a worldwide prevalence in the general population of 5% to 12% and is divided phenotypically into two main groups: Chronic Rhinosinusitis without Nasal Polyposis and Chronic Rhinosinusitis with Nasal Polyposis, presenting processes distinct remodeling. Involved in the remodeling process, the BMP-7, MMP-9 and TGF-β proteins appear to exhibit different behaviors in the two phenotypes and different relationships with the interleukins IL-6 and IL-10, which will be investigated in this study. Objective: to evaluate the expression of BMP-7, MMP-9, TGF-β protein in Rhinosinusitis with and without Nasal Polyposis and its relationship with interleukins IL-6 and IL-10. Method: the sample consisted of 86 participants, divided into three groups: one of patients with Chronic Rhinosinusitis With Nasal Polyposis (n = 34), one of patients with Chronic Rhinosinusitis without Nasal Polyposis (n= 26), both groups according to EPOS 2012 criteria, and a control group without inflammatory nasal mucosa disease (n = 26). BMP-7, MMP-9, TGF-β, IL-6 and IL-10 were dosed into the tissue by Elisa method. The presence of eosinophils was evaluated by immunohistochemical Hematoxylin/ Eosin. Results: the high-power field (HPF) eosinophil count in the Nasal Polyp sample was above 5 eosinophils per high-power field in 91% of the cases and 73% presented more than 10 eosinophils by High-Power Field. TGF-β level were significantly lower in the polypoid tissue group when compared to control group and Chronic Rhinosinusitis without Nasal Polyposis group. Dosage of IL-6 was significantly higher in the Nasal Polyps group when compared to the other groups. Dosage of IL-10 was significantly higher in control group when compared to the other groups. There were significant negative correlations between IL-6 and IL-10 in all group, and between MMP-9 and IL-6 in control group and Nasal Polyp group. There was a positive correlation between MMP-9 and IL-10 in Nasal Polyp group. There was a positive correlation between BMP-7 and IL-10 in all groups. Finally, the correlation between TGF-β and IL-6 was positive in control group and negative in Chronic Rhinosinusitis without Nasal Polyposis group. Conclusion: In Chronic Rhinosinusitis without Nasal Polyposis there is a low concentration of IL-10 and a high concentration of TGF-β. In Nasal Polyposis there is a low concentration of TGF-β and IL-10 and an increase in IL-6. IL-6 and IL-10 have a negative correlation with each other and distinct interactions with BMP-7 and MMP-9 in the groups analyzed. We concluded, therefore, that there is a close relationship between interleukins 6 and 10 and the main proteins involved in the remodeling process in Chronic Rhinosinusitis.