Navegando por Palavras-chave "Organ Preservation Solutions"

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    Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-03-01) Perez, Rogerio Renato; Goldenberg, Alberto [UNIFESP]; Netto, Alcides Augusto Salzedas; Gonzalez, Adriano Miziara [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    PURPOSE:To compare the efficacy of different types of solutions (Belzer or Euro-Collins) for the preservation of rat pancreas during cold ischemia. METHODS:Thirty Wistar rats were divided into three groups according to the perfusion or storage solution: Group E (perfusion and storage in Euro-Collins solution); Group B (perfusion and storage in Belzer solution) and Group BE (Perfusion in Belzer solution and storage in Euro-Collins solution). After perfusion, the pancreas was excised and stored at 4˚C for 18 hours. Amylase was measured at 6, 12 and 18h, and histological analysis of the pancreas was performed after 18h of cold storage.RESULTS:Amylase was elevated and comparable in Groups E and BE after 12 and 18 hours of ischemia (p<0.05). In the exocrine pancreas, histological differences in the amount of necrosis (p=0.049), lymphocytic infiltrate (p<0.001) and neutrophilic infiltrate (p=0.004) were observed, with more favorable features present in Group B. In the endocrine pancreas, Group B showed less edema (p<0.001), but other parameters were similar among all groups. CONCLUSION:The Euro-Collins solution is inferior to the Belzer solution for the preservation of rat pancreas during cold ischemia.
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    Effects of ischemic preconditioning associated to different preservation solutions in protecting the intestinal graft
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2011-10-01) Neves, José de Souza [UNIFESP]; Abrahão, Marcos de Souza [UNIFESP]; Salzedas-Netto, Alcides Augusto [UNIFESP]; Montero, Edna Frasson de Souza [UNIFESP]; Gonzalez, Adriano Miziara [UNIFESP]; University of Mato Grosso Department of Surgery; Universidade Federal de São Paulo (UNIFESP)
    PURPOSE: To evaluate the effects of ischemic preconditioning (IPC) associate with different preservation solutions, in the protecting of gut. METHODS: Four groups of 14 rats underwent laparotomy and collecting 20 cm of ileum, for preservation, at 4ºC, in Belzer (Belz), Ringer (RL), Celsior (Cs) and Custodiol (Cust) solutions, for 24 hours. Prior to collection, half of the animals in each group were subjected to IPC. During preservation, in the periods of zero, 12, 18 and 24 hours, were conducted evaluating the degree of mucosal injury and dosage of malondialdehyde acid (MDA). RESULTS: In all periods the RL group, with and without IPC, presented MDA values higher than the Belz and Cs. The degree of mucosal injury in the non-ipc RLgroup with 12h preservation was higher than the others; with 18 and 24h, the RL and Cust had higher degrees of damage than Cs and Belz. With IPC, in all periods, the group Cs and Belz had lower degrees of injury. CONCLUSION: The Celsior and Belzer solutions had better protective effects on the gut and these effects were enhanced by IPC.