Navegando por Palavras-chave "Neonatal Period"

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    Avaliação dos efeitos da inflamação sistêmica no período neonatal sobre o cerebelo na vida adulta
    (Universidade Federal de São Paulo (UNIFESP), 2019-08-29) Pires, Jaime Moreira [UNIFESP]; Mello, Luiz Eugenio Araujo De Moraes [UNIFESP]; Foresti, Maira Licia [UNIFESP]; http://lattes.cnpq.br/5451196671533628; http://lattes.cnpq.br/4462750801249231; http://lattes.cnpq.br/2487629230355637; Universidade Federal de São Paulo (UNIFESP)
    Objectives: To evaluate the chronic effects of a systemic inflammatory process in the cerebellum during the neonatal period. Methodology: Wistar norvegicus (male) rat pups were distributed in the NAIVE, SHAM and LPS groups. Treated animals received either sodium chloride 0.9% or lipopolysaccharide solution intraperitoneally on the first, third, fifth and seventh postnatal days. On the postnatal day 84, the animals were submitted to the open field test to evaluate the locomotor activity. On postnatal day 89, some animals were perfused with fixative, and the cerebellum was processed for Purkinje and microglia cells immunofluorescence, and for ethidine histochemistry for the detection of superoxide anion and evaluation of oxidative stress. Other animals were euthanized by decapitation, to evaluate expression of the inflammatory cytokine TNF-α in the cerebellum and hippocampus. Results: Animals exposed to neonatal systemic inflammation (LPS group) had higher density of microglia cells and greater superoxide anion production in the adult phase when compared to the control groups (NAIVE and SHAM groups). No significant differences were found regarding locomotor activity, Purkinje cell density and TNF-α expression. Conclusion: Exposure to systemic inflammatory processes in the neonatal phase may have chronic effects on the cerebellum. Being an important structure for motor, executive, as well as cognitive and emotional functions, further studies are necessary to understand the mechanisms by which injuries in the neonatal period can result in changes last through life.