Navegando por Palavras-chave "Muscle physiology"

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    Histological changes underlying bupivacaine's effect on extra ocular muscle
    (Academic Press Ltd- Elsevier Science Ltd, 2018) Hopker, Luisa Moreira [UNIFESP]; Neves, Juliana de Carvalho; Nascimento, Daiane Jaqueline; Campos, Eliene Dutra; Mendonca, Tomas Scalamandre [UNIFESP]; Zanoteli, Edmar; Allemann, Norma [UNIFESP]
    To determine the changes in the cross-sectional area (CSA) of myofibers and their subtype distribution based on the myosin isoform expression after bupivacaine (BUP) injection in the EOM of rabbits and help the understanding of strabismus correction after BUP injection in the clinical practice. A total of 32 rabbits received 0.3 mL of 1.5% BUP in the superior rectus muscle (SR) of the right eye (OD) and were sacrificed at days 7, 28, 60, and 92. Additional eight untouched rabbits were included as controls. Hematoxylin and eosin staining was performed, and ImageJ software was used to measure CSA. Immunohistochemical analysis was performed to analyze the proportion of myofibers positive for myosin types 1 (slow), 2 (fast) and embryonic. Myofiber area measurement decreased 7 days after BUP injection [SR, 1271 +/- 412 mu m(2) (control) to 909 +/- 255 mu m(2) (day 7)] after BUP injection, followed by an increasing trend after 28 days and normalization after 92 days [SR