Navegando por Palavras-chave "Muscle atrophy"

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    Adaptações musculares induzidas pela privação de sono
    (Universidade Federal de São Paulo (UNIFESP), 2018-06-28) Mônico-Neto, Marcos [UNIFESP]; Antunes, Hanna Karen Moreira [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Aims: To evaluate the effects of sleep deprivation and recovery on the muscle regeneration process and muscle IGF-1 concentrations in rats submitted to cryolesion. The present study also evaluated the histopathological changes, oxidative damage and the modulatory effects of corticosterone on different types muscle fibers in sleep deprived rats. Methods: Male Wistar rats, 3-month-old, were submitted to cryolesion of the anterior tibial muscle and after 4 groups were established: control group (CTL, n=8), sleep deprivation group for 96h (SD96, n=8), group CTL + sleep recovery period (CTL+R, n=8) and SD96 + sleep recovery period for 96h (PS96+R, n=8). The SD96 and SD96+R groups were submitted to sleep deprivation for 96h and in the end; the PS96+R group remained for another 96h with sleep ad libitum. Control groups remained in the housing box for the same period of sleep deprivation and sleep recovery. The muscle IGF-1, hormone profile (testosterone and corticosterone), PCNA protein expression and histopathological changes of the tibialis anterior muscle were analyzed. A second experiment distributed animals of the same lineage and age into three groups, the CTL group treated with vehicle (CTL, n = 10), SD treated with metyrapone (SD+MET, n=10) and SD treated with vehicle (SD+VEI, n=10). The metyrapone drug is corticosterone synthesis inhibitor and propyleneglycol was used as vehicle. The soleus muscle (oxidative fibers) and plantaris muscle (glycolytic fibers) were analyzed for histopathological pattern, oxidative damage, mitochondrial and lysosomal activity. Results: Sleep deprivation reduced muscle IGF-1, minimized its increase in the injured muscle, and sleep recovery was effective in restoring growth factor concentrations. A delay in the muscle regeneration process was observed in the animals of the SD96+R group when compared to the CTL+R group. When comparing the different types muscle fibers, pathological processes were observed in sleep deprived animals, in both analyzed muscles (soleus and plantaris), being more intense in the soleus, with interstitial edema and tissue degeneration. Oxidative damage was observed in both muscles, being more intense in the soleus muscle. Oxidative damage and lysosomal activity increased in the SD+VEI group only in the soleus muscle and, lysosomal activity increased in the SD+MET group only in the plantaris muscle. Conclusions: Sleep deprivation impairs the muscle regeneration process in rats and reduces muscle IGF-1 concentrations. Sleep recovery restored the hormonal pattern, but it was not enough to normalize the process of muscle regeneration. Histopathological changes induced by sleep deprivation in the skeletal muscle occur according to the type of muscle fiber, and type I fibers undergo greater oxidative damage. In addition, the data suggest that corticosterone potentiates oxidative damage in the soleus muscle and muscle fiber type seems to be determinant for the outcome of corticosterone effects during sleep deprivation.