Navegando por Palavras-chave "Matrigel"

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    Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration
    (Medknow Publications & Media Pvt Ltd, 2018) Fernandes, Marcela [UNIFESP]; Valente, Sandra Gomes [UNIFESP]; Sabongi, Rodrigo Guerra [UNIFESP]; Gomes dos Santos, Joao Baptista [UNIFESP]; Leite, Vilnei Mattioli [UNIFESP]; Ulrich, Henning; Nery, Arthur Andrade; da Silva Fernandes, Maria Jose [UNIFESP]
    Studies have confirmed that bone marrow-derived mesenchymal stem cells (MSCs) can be used for treatment of several nervous system diseases. However, isolation of bone marrow-derived MSCs (BMSCs) is an invasive and painful process and the yield is very low. Therefore, there is a need to search for other alterative stem cell sources. Adipose-derived MSCs (ADSCs) have phenotypic and gene expression profiles similar to those of BMSCs. The production of ADSCs is greater than that of BMSCs, and ADSCs proliferate faster than BMSCs. To compare the effects of venous grafts containing BMSCs or ADSCs on sciatic nerve injury, in this study, rats were randomly divided into four groups: sham (only sciatic nerve exposed), Matrigel (MG
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    Inhibitory effect of the sugarcane cystatin CaneCPI-4 on cathepsins B and L and human breast cancer cell invasion
    (Walter de Gruyter & Co, 2008-04-01) Gianotti, Andreia; Sommer, Csar A.; Carmona, Adriana K. [UNIFESP]; Henrique-Silva, Flavio; Universidade Federal de São Carlos (UFSCar); Universidade Federal de São Paulo (UNIFESP)
    The lysosomal cysteine proteases cathepsin B and L play important roles in tumor cell invasion. An imbalance between these cathepsins and their endogenous inhibitors, the cystatins, has been associated with development of the metastatic phenotype. Accordingly, many studies have indicated potential use of cystatins in therapeutic approaches. We report a novel cystatin from sugarcane (Saccharum officinarum), CaneCPI-4, with strong inhibitory activity against cathepsins B (K-i=0.83 nm) and L (K-i=0.021 nm). the invasive ability of MDA-MB-231 human breast cancer cells expressing CaneCPI-4 was only slightly decreased. in contrast, addition of low, non-toxic concentrations of recombinant His-tagged CaneCPI-4 significantly reduced invasion through a Matrigel matrix. Immunoblot analyses failed to detect the recombinant protein inside cells, indicating that the cystatin was not internalized by endocytosis, but exerted its anti-invasive effect mainly through inhibition of extracellular cathepsins. Our findings open the possibility of considering phytocystatins for anti-cancer strategies.