Navegando por Palavras-chave "Brewery waste"

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    Bioconversion of low-cost brewery waste to biosurfactant: an improvement of surfactin production by culture medium optimization
    (Colin Webb, 2021-05-11) Corrêa Nazareth, Talita; Planas Zanutto, Conrado; Maass, Danielle [UNIFESP]; Ulson de Souza, Antônio Augusto; Guelli Ulson de Souza, Selene Maria de Arruda; http://lattes.cnpq.br/3431482044015696
    Trub is the second-largest residue from brewing industry (250 thousand tons/year) and, due to the presence of high amounts of carbon and nitrogen, it is a potential source of reducing sugars and nitrogen for biological processes. Therefore, this work aimed to enhance surfactin production by B. subtilis using Trub as a carbon source. The effect of nitrogen sources on biosurfactant production was investigated by a Full Factorial Design (2²). Yeast extract and its interaction with peptone presented a statistically significant effect on the response variable of surface tension (ST). ST was described by a linear regression model (R² = 0.93). The effect of metals supplementation on the culture medium was also investigated, where the volumetric productivity increased from 2.98 to 7.43 mg.L-1.h-1 for the medium without (M-I) and with metals (M-II), respectively. After 28h, 210.11 mg.L-1 was obtained in the batch culture with MII medium, being higher than the maximum surfactin concentration achieved after 52 h (121.80 mg.L-1) for M-I medium. The biosurfactant production in bioreactor was successfully performed since a concentrated extract was obtained in the foam collected, reaching the maximum of 1118.20 mg.L-1 of surfactin.
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    Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate
    (Elsevier, 2021-03-15) Zanutto, Talita Corrêa Nazareth; Zanutto, Conrado Planas; Maass, Danielle [UNIFESP]; Souza, Antônio Augusto Ulson de; Souza, Selene Maria de Arruda Guelli Ulson de; http://lattes.cnpq.br/3431482044015696
    This work reports surfactin production by B. subtilis ATCC 6051 in a stirred tank bioreactor, where trub was used as a carbon source. Trub is a residue from the brewing industry mainly composed of high-molar-mass proteins and reducing sugars, being a sustainable and low-cost substrate for bioprocess. Despite this, Trub has been little explored as a raw material in bioprocesses. Aiming to evaluate important factors in scale-up process, the impact of oxygen transfer coefficient (KLa) concerning volumetric productivity, and the effect of agitation (150–300 rpm) and aeration (0.5–1.5 vvm) on biosurfactant production by a Full Factorial design were evaluated. Only agitation presented a significant effect on surfactin concentration (SF), where SF was described by a linear regression model (R2 = 0.99). The biosurfactant showed high stability in relation to variations in temperature and pH. In addition, the foam collecting was an efficient method in recovering and enrichment of the product.
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    A low‑cost brewery waste as a carbon source in bio‑surfactant production
    (Dirk Weuster-Botz, 2021-06-22) Corrêa Nazareth, Talita; Planas Zanutto, Conrado; Maass, Danielle [UNIFESP]; Ulson de Souza, Antônio Augusto; Guelli Ulson de Souza, Selene Maria de Arruda; http://lattes.cnpq.br/3431482044015696
    This work aims to produce bio-surfactant using a brewery waste (trub) as a strategy to reduce production costs related to the substrate, as well as to provide an eco-friendly destination for this residue. Trub is obtained during the boiling of the wort, being mainly composed of proteins and reducing sugars. To evaluate important process parameters on bio-surfactant production, a full factorial design (2^4) was elaborated, having agitation rate and concentrations of trub, yeast extract, and peptone as independent variables. The highest bio-surfactant concentration achieved was 100.76 mg L−1, where FTIR and Maldi-ToF-MS confirmed functional groups characteristic of peptides and isomers of surfactin in the biosurfactant extract. Trub, agitation and yeast extract showed statistically significant effects on the response variable (surface tension), where an increase in the agitation rate and in the concentration of yeast extract demonstrated a positive impact on the production of bio-surfactant.
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    The use of low-cost brewery waste product for the production of surfactin as a natural microbial biocide
    (Elsevier, 2020-10-07) Nazareth, Talita Corrêa; Zanutto, Conrado Planas; Tripathi, Lakshmi; Juma, Abdulaziz; Maass, Danielle [UNIFESP]; Souza, Antônio Augusto Ulson de; Souza, Selene Maria de Arruda Guelli Ulson de; http://lattes.cnpq.br/3431482044015696
    Surfactin has potential as next generation antibiofilm agent to combat antimicrobial resistance against emerging pathogens. However, the widespread industrial applications of surfactin is hampered by its high production cost. In this work, surfactin was produced from Bacillus subtilis using a low-cost brewery waste as a carbon source. The strain produced 210.11 mg L-1 after 28 h. The antimicrobial activity was observed against all tested strains, achieving complete inhibition for Pseudomonas aeruginosa, at 500 mg mL-1. A growth log reduction of 3.91 was achieved for P. aeruginosa while, Staphylococcus aureus and Staphylococcus epidermidis showed between 1 and 2 log reductions. In the anti-biofilm assays against P. aeruginosa, the co-incubation, anti-adhesive and disruption showed inhibition, where the greatest inhibition was observed in the co-incubation assay (79.80%). This study provides evidence that surfactin produced from a low-cost substrate can be a promising biocide due to its antimicrobial and anti-biofilm abilities against pathogens.