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Title: Alprostadil attenuates inflammatory aspects and leucocytes adhesion on renal ischemia and reperfusion injury in rats
Authors: Soares, Bruno Leonardo de Freitas [UNIFESP]
Freitas, Maria Andréia Lopes de
Montero, Edna Frasson de Souza [UNIFESP]
Pitta, Guilherme Benjamin Brandão
Miranda Júnior, Fausto
Universidade Federal de São Paulo (UNIFESP)
Federal University do Vale do São Francisco Faculty of Nursing
University of São Paulo Faculty of Medicine Department of Surgery
University of Health Sciences of Alagoas Faculty of Medicine
Keywords: ischemic preconditioning
Oxidative stress
Issue Date: 1-Jan-2014
Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Citation: Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 55-60, 2014.
Abstract: PURPOSE:To evaluate the effects of alprostadil in an experimental model of ischemia and reperfusion injury (IRI) in rat renal tissue.METHODS: Adult male Wistar rats were randomized into three groups Vehicle-treated group(Veh), Alprostadil-treated(Al), and sham(Sh) group. Veh and Al groups had suprarenal aorta occluded for 30 minutes and reperfused for 60 minutes. Saline or 20 µg/kg of Alprostadil was intravenously infused immediately before declamping. Sh group animals underwent similar procedure without aortic occlusion. Left nephrectomy and blood sampling were performed after 60 minutes of reperfusion. Renal ICAM-1 expression and histological analysis were performed to estimate inflammatory response and tissue disarrangement. Serum biochemical markers for IRI were also measured. Kruskal-Wallis test was used to assess differences between the groups.RESULTS: There was lower expression of ICAM-1 in groups Veh and Sh. On histologically evaluation, inflammation and necrosis in the Veh group was significantly higher (grades III/IV) than Al group (Veh>Al=Sh; p = 0.025), as well as CPK levels (Veh>Al=Sh; p = 0.03).CONCLUSION: Alprostadil attenuates the immunohistochemical and histological repercussions in the renal tissue of rats submitted to a post-ischemic reperfusion with supra-renal aortic clamping.
ISSN: 0102-8650
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