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Title: A manual method to obtain platelet rich plasma
Authors: Marques, Fabiana Paulino
Ingham, Sheila Jean McNeill [UNIFESP]
Forgas, Andrea
Franciozi, Carlos Eduardo da Silveira [UNIFESP]
Sasaki, Pedro Henrique
Abdalla, Rene Jorge [UNIFESP]
Hospital do Coração Knee Institute
Universidade Federal de São Paulo (UNIFESP)
Keywords: Platelet-rich plasma
Blood platelets
Issue Date: 1-Jan-2014
Publisher: Sociedade Brasileira de Ortopedia e Traumatologia
Citation: Acta Ortopédica Brasileira. Sociedade Brasileira de Ortopedia e Traumatologia, v. 22, n. 2, p. 75-77, 2014.
Abstract: OBJECTIVE:This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS:For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS:A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION:The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor.Level of Evidence IV, Controlled Laboratory Study.
ISSN: 1413-7852
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Appears in Collections:Artigo

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